Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,472,204 T→A 9.7% K95M (AAG→ATG) ‡ rlmH ← 23S rRNA (pseudouridine(1915)‑N(3))‑methyltransferase RlmH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,472,2040TA 9.7% 144.9 / 7.2 52K95M (AAG→ATG) ‡rlmH23S rRNA (pseudouridine(1915)‑N(3))‑methyltransferase RlmH
Reads supporting (aligned to +/- strand):  ref base T (23/23);  new base A (3/2);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01

GCGACAGCGACCAACGTTGCTCGGCGCGCGCGCAAACCTCAGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGACAGC  >  NC_002947/5472064‑5472347
                                                                                                                                            |                                                                                                                                               
gcgACAGCGACCAACGTTGCTCGGCGCGCGCGCAAACCTCAGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGa                                                                                                                                        <  1:1043701/150‑1 (MQ=255)
gcgACAGCGACCAACGTTGCTCGGCGCGCGCGCAAACCTCAGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCAAGCCATGGa                                                                                                                                        <  2:689775/150‑1 (MQ=255)
   aCAGCGACCAACGTTGCTCGGCGCGCGCGCAAACCTCAGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCt                                                                                                                                     >  1:707765/1‑150 (MQ=255)
     aGCGACCAACGTTGCTCGGCGCGCGCGCAAACCTCAGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCTTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTGCTCCATGGCTTGCCCTAGACCTCg                                                                                                                                   >  1:521114/1‑150 (MQ=255)
      gCGACCAACGTTGCTCGGCGCGCGCGCAAACCTCAGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGa                                                                                                                                  >  1:1036760/1‑150 (MQ=255)
            aaCGTTGCTCGGCGCGCGCGCAAACCTCAGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGa                                                                                                                            <  1:1065386/150‑1 (MQ=255)
               gTTGCTCGGCGCGCGCGCAAACCTCAGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATg                                                                                                                                          >  2:616807/1‑133 (MQ=255)
               gTTGCTCGGCGCGCGCGCAAACCTCAGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATg                                                                                                                                          <  1:616807/133‑1 (MQ=255)
                  gCTCGGCGCGCGCGCAAACCTCAGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGc                                                                                                                      <  2:371929/150‑1 (MQ=255)
                      ggCGCGCGCGCAAACCTCAGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTc                                                                                                                  <  2:117494/150‑1 (MQ=255)
                                     cTCAGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACttt                                                                                                   >  2:1029712/1‑150 (MQ=255)
                                     cTCAGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACttt                                                                                                   >  1:605342/1‑150 (MQ=255)
                                      tCAGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATCGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTg                                                                                                  >  2:687145/1‑150 (MQ=255)
                                          gcgcCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCa                                                                                              <  1:666802/150‑1 (MQ=255)
                                              cAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCAt                                                                                          >  2:511862/1‑150 (MQ=255)
                                               aGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACttt                                                                                                   <  2:254783/140‑1 (MQ=255)
                                               aGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACttt                                                                                                   >  1:254783/1‑140 (MQ=255)
                                               aGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATg                                                                                         >  2:909485/1‑150 (MQ=255)
                                                    ccccGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTc                                                                                    <  2:764895/148‑1 (MQ=255)
                                                     tCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCg                                                                                   >  1:1110994/1‑150 (MQ=255)
                                                      ccGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGc                                                                                  <  2:772196/150‑1 (MQ=255)
                                                              cccACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGa                                                                          >  1:914017/1‑150 (MQ=255)
                                                              cccACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGa                                                                          >  2:388355/1‑150 (MQ=255)
                                                              cccACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCAAGCCATg                                                                                                                                          <  2:470361/86‑1 (MQ=255)
                                                              cccACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCAAGCCATg                                                                                                                                          >  1:470361/1‑86 (MQ=255)
                                                                   caTCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCa                                                                                                              <  1:831798/109‑1 (MQ=255)
                                                                   caTCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCa                                                                                                              >  2:831798/1‑109 (MQ=255)
                                                                   caTCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGAt                                                                     >  2:335170/1‑150 (MQ=255)
                                                                    aTCAAATTCACCGTGCGCGCATCCAGGCGCCAGAGGTCCGTATCTGTCGCGAGCTGCTCGGTACTCCATGTCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATc                                                                    >  1:809554/1‑150 (MQ=255)
                                                                     tCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAAt                                                                                                                        >  2:146102/1‑97 (MQ=255)
                                                                     tCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAAt                                                                                                                        <  1:146102/97‑1 (MQ=255)
                                                                     tCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGCACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCCGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCa                                                                   >  1:237762/1‑150 (MQ=255)
                                                                     gcAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCa                                                                   <  2:1114469/149‑1 (MQ=255)
                                                                          gtcgccGTGCGCGCCTCCAGGCGCAAGCGGTCGAGGTCGGTAGCCAGATGTTCGGTACTCCATGGCTTGCCATGGACCTCGATGGTGACAATGCGTTCCCCAGGCTGCACGATGCTCAGCATGGCTTCGGCCTCCTGGAGGATCAggcag                                                              <  2:521114/146‑3 (MQ=255)
                                                                                    gcgcATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCgg                                                    <  1:511862/150‑1 (MQ=255)
                                                                                           cAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCtt                                             <  1:883154/150‑1 (MQ=255)
                                                                                            aGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTg                                            >  2:192502/1‑150 (MQ=255)
                                                                                                gcCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCAc                                        <  2:707765/150‑1 (MQ=255)
                                                                                                  cAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACgg                                      <  1:1029712/150‑1 (MQ=255)
                                                                                                  cAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACgg                                      <  2:1036760/150‑1 (MQ=255)
                                                                                                            gCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCg                            <  1:909485/150‑1 (MQ=255)
                                                                                                            gCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGCCCACATTCTTGCCACGGGTATTCAGCg                            <  1:151096/150‑1 (MQ=255)
                                                                                                                 gTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGAtt                       >  1:80597/1‑150 (MQ=255)
                                                                                                                  tCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGAttt                      <  2:153273/150‑1 (MQ=255)
                                                                                                                      cAGCTGCCCGGTACTCCATGGCGTGCCATGGACCTCGAGGGTGACAATGAGTCCCCCAGGCTGCACTGTGCTCAGCACGGCTTCGCCCTCCTGACGGTTCAGGCGGGCGACGCCGGCATTCTTGCCACGGGTATTCAGCGGGATTTcccc                  <  2:194090/150‑3 (MQ=255)
                                                                                                                       aGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGt                                                       <  2:822367/112‑1 (MQ=255)
                                                                                                                       aGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGt                                                       >  1:822367/1‑112 (MQ=255)
                                                                                                                        gctgctCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTccacca                <  2:1110994/150‑1 (MQ=255)
                                                                                                                             tCGGTACTCCATGGCTTGCCATGGACCTCNAGGGTGACAATTCGNTCCCNAGGCTGCACTTTGCTCAGCATGGCCGCGCCCTCCTGTCGGATCAGGCGGGCGACGTCGGCATTATTGCCACGGGCATTCAGCGGGATTTCCACCAACTcc           >  1:25221/1‑150 (MQ=255)
                                                                                                                                 aggtCCATGGCAAGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTCCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCg       >  2:791497/4‑150 (MQ=255)
                                                                                                                                  ggtCCATAGCAAGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGa      >  2:590882/3‑150 (MQ=255)
                                                                                                                                      cATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGACAGc  >  2:348881/1‑150 (MQ=255)
                                                                                                                                            |                                                                                                                                               
GCGACAGCGACCAACGTTGCTCGGCGCGCGCGCAAACCTCAGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGACAGC  >  NC_002947/5472064‑5472347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑