Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 484,691 C→T 9.8% intergenic (‑250/+33) yeaG ← / ← glpE protein kinase/thiosulfate sulfurtransferase GlpE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947484,6910CT 9.8% 104.7 / 3.4 41intergenic (‑250/+33)yeaG/glpEprotein kinase/thiosulfate sulfurtransferase GlpE
Reads supporting (aligned to +/- strand):  ref base C (17/20);  new base T (2/2);  total (19/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.52e-01

TAACGCTACCGATGACCCGCACGCCGGTGTACCGGCTCTCCCCTTTTTGGATGGCCTGAGCTTAAGCGTAGTTGGTTATCTGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGAAGCCTCCGTCCACGCTGTACACGTCGGAGAAACCCTGGCCTACCAGGTAGGCGGCAGCACT  >  NC_002947/484544‑484840
                                                                                                                                                   |                                                                                                                                                     
tAACGCTACCGATGACCCGCACGCCGGTGTACCGGCTCTCCCCTTTTTGGATGGCCTGAGCTTAAGCGTAGTTGGTTATCTGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCcgcgc                                                                                                                                                     >  2:1118574/1‑150 (MQ=255)
             taCCCGCACGCCGGTGTACCGGCTCTCCCCTTTTTGGATGGCCTGAGCTTAAGCGTAGTTGGTTATCTGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGTGCGCCGCGCGGGCtt                                                                                                                                        <  1:661688/149‑2 (MQ=255)
              aCCCGCACGCCGGTGTACCGGCTCTCCCCTTTTTGGATGGCCTGAGCTTAAGCGTAGTTGGTTATCTGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGc                                                                                                                                       <  1:7607/150‑1 (MQ=255)
                       ccGGTGTACCGGCTCTCCCCTTTTTGGATGGCCTGAGCTTAAGCGTAGTTGGTTATCTGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGaaaa                                                                                                                              <  1:249855/150‑1 (MQ=255)
                       ccGGTGTACCGGCTCTCCCCTTTTTGGATGGCCTGAGCTTAAGCGTAGTTGGTTATCTGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGaaaa                                                                                                                              <  2:792064/150‑1 (MQ=255)
                                    tctcCCCTTTTTGGATGGCCTGAGCTTAAGCGTAGTTGGTTATCTGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCCAGCCCGCGCGTCGCACGGGCTGCAGGGGAAAATAAAAAATTATTc                                                                                                                 <  1:213941/150‑1 (MQ=255)
                                        cccTTTTTGGATGGCCTGAGCTTAAGCGTAGTTGGTTATCTGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCg                                                                                                             >  1:487803/1‑150 (MQ=255)
                                                       cTGAGCTTAAGCGTAGTTGGTTATCTGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTAAGATAAGCCGCAAGCCCGCGCGGCTCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGtc                                                                                              >  2:678574/1‑150 (MQ=255)
                                                             ttAAGCGTAGTTGGTTATCTGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGcc                                                                                        >  1:916626/1‑150 (MQ=255)
                                                             ttAAGCGTAGTTGGTTATCTGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGcc                                                                                        >  1:553092/1‑150 (MQ=255)
                                                                 gCGTAGTTGGTTATCTGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTgg                                                                                                    >  2:398073/1‑134 (MQ=255)
                                                                 gCGTAGTTGGTTATCTGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTgg                                                                                                    <  1:398073/134‑1 (MQ=255)
                                                                 gCGTAGTTGGTTATCTGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGt                                                                                    <  2:916626/150‑1 (MQ=255)
                                                                    tAGTTGGTTATCTGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAgg                                                                                 <  1:1118574/150‑1 (MQ=255)
                                                                       ttGGTTATCTGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCt                                                                                                      >  1:731456/1‑126 (MQ=255)
                                                                       ttGGTTATCTGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCt                                                                                                      <  2:731456/126‑1 (MQ=255)
                                                                          gTTATCTGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTcgcgc                                                                           <  1:295219/150‑1 (MQ=255)
                                                                                tGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCcaca                                                                     >  2:176385/1‑150 (MQ=255)
                                                                                tGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCcaca                                                                     <  2:139770/150‑1 (MQ=255)
                                                                                tGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCACGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGGAGGTCGCGCGCcaca                                                                     >  2:70558/1‑150 (MQ=255)
                                                                                     ggTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCg                                                                                                 <  1:613980/117‑1 (MQ=255)
                                                                                     ggTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCg                                                                                                 >  2:613980/1‑117 (MQ=255)
                                                                                       tAANGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGaa                                                              >  2:699886/1‑150 (MQ=255)
                                                                                       tAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGaa                                                              <  1:766706/150‑1 (MQ=255)
                                                                                       tAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGaa                                                              <  2:213450/150‑1 (MQ=255)
                                                                                        aaaGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCa                                                                                                                >  2:949562/1‑99 (MQ=255)
                                                                                        aaaGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCa                                                                                                                <  1:949562/99‑1 (MQ=255)
                                                                                        aaaGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGAAg                                                             <  1:414202/150‑1 (MQ=255)
                                                                                                   aaaGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGAAGCCTCCGTCCAc                                                  <  2:487803/150‑1 (MQ=255)
                                                                                                     aGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCACTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGAAGCCTCCGTCCACGc                                                >  2:1052235/1‑150 (MQ=255)
                                                                                                              ttACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGAAGTTTCCGTTCACGCCGTACACGt                                       >  2:526417/1‑150 (MQ=255)
                                                                                                               tACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGAAGCCTCCGTCCACGCTGTACACGTc                                      <  2:967846/150‑1 (MQ=255)
                                                                                                                        ccGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGAAGCCTCCGTCCACGCTGTACACGTCGGAGAAAcc                             >  2:376987/1‑150 (MQ=255)
                                                                                                                        ccGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGAAGCCTCCGTCCACGCTGTACACGTCGGAGAAAcc                             >  1:82202/1‑150 (MQ=255)
                                                                                                                          gTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAgg                                                                                 <  2:1124655/96‑1 (MQ=255)
                                                                                                                          gTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAgg                                                                                 >  1:1124655/1‑96 (MQ=255)
                                                                                                                                          gCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGAAGCCTCCGTCCACGCTGTACACGTCGGAGAAACCCTGGCCTACCAGGTAggc           >  1:848802/1‑150 (MQ=255)
                                                                                                                                              gCCCGTGCGCCGCGCGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGAAGCCTCCGTCCACGCTGTACACGTCGGAGAAACCCTGGCCTACCAGGTa              <  1:91231/143‑1 (MQ=255)
                                                                                                                                              gCCCGTGCGCCGCGCGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGAAGCCTCCGTCCACGCTGTACACGTCGGAGAAACCCTGGCCTACCAGGTa              >  2:91231/1‑143 (MQ=255)
                                                                                                                                              gCCCGTGCGCCGCGCGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGAAGCCTCCGTCCACGCTGTACACGTCGGAGAAACCCTGGCCTACCAGGTAGGCGgca       >  2:479846/1‑150 (MQ=255)
                                                                                                                                              gCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGAAGCCTCCGTCCACGCTGTACACGTCGGAGAAACCCTGGCCTACCAGGTAGGCGgca       <  2:82202/150‑1 (MQ=255)
                                                                                                                                              gCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGAAGCCTCCGTCCACGCTGTACACGTCGGAGAAACCCTGGCCTACCAGGTAGGCGgca       <  1:37485/150‑1 (MQ=255)
                                                                                                                                                   cgcgGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGAAGCCTCCGTCCACGCTGTACACGTCGGAGAAACCCTGGCCTACCAGGTAGGCGGCAGCACt  >  2:887536/1‑150 (MQ=255)
                                                                                                                                                   cgcgGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGAAGCCTCCGTCCACGCTGTACACGTCGGAGAAACCCTGGCCTACCAGGTAGGCGGCAGCACt  <  1:699886/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TAACGCTACCGATGACCCGCACGCCGGTGTACCGGCTCTCCCCTTTTTGGATGGCCTGAGCTTAAGCGTAGTTGGTTATCTGCAAGGTAAAGGGGTGAGAAAGGCAGAAGTTACGAACGACCGTTCGTCAGATAAGCCGCAAGCCCGCGCGGCGCACGGGCTGCAGGGGAAAATAAAAAATTATTCAGCGTTGCCCTGGGCGGTCTCGGCCGGGTAGGTCGCGCGCCACAGTTCGAAGCCTCCGTCCACGCTGTACACGTCGGAGAAACCCTGGCCTACCAGGTAGGCGGCAGCACT  >  NC_002947/484544‑484840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑