Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,704,960 G→A 9.1% P157S (CCA→TCA)  PP_5007 ← polyhydroxyalkanoate granule‑associated protein GA2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,704,9600GA 9.1% 114.6 / 3.3 44P157S (CCA→TCA) PP_5007polyhydroxyalkanoate granule‑associated protein GA2
Reads supporting (aligned to +/- strand):  ref base G (18/22);  new base A (2/2);  total (20/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.10e-01

CAGGTTTGGCCGCTGCAGGCTTGGCTGCTGGCTTAGCTGCAGGTTTTTCCGCTGCGGTTTTGGCTGCGGGTTTGGCCGCTGCGGTTTTGCCAGCAGGTTTG‑‑‑GCCGCCGCCGTTTTCGCCGCAGGCTTAGCTGCTGCCTTGGCCAGTGGCTTGGCCGCAGCCTTGCTTGCAGCCGGCTTGGTTGCTGCAGCGCGCGACGAAATCGGCGTAACCGAAGCACCGGTCAGCTTCTCGATCTGCTTGGTCAGGCTGTCCACCTGCTGGTGCAGTGCCTTGATCTCGTTGCGG  >  NC_002947/5704813‑5705099
                                                                                                                                                      |                                                                                                                                           
cAGGTTTGGCCGCTGCAGGCTTGGCTGCTGGCTTAGCTGCAGGTTTTTCCGCTGCGGTTTTGGCTGCGGGTTTGGCCGCTGCGGTTTTGCCAGCAGGTTTG‑‑‑GCCGCCGCCGTTTTCGCCGCAGGCTTAGCTGCTGCCTTGGCCAGTGGCt                                                                                                                                           >  1:1034335/1‑150 (MQ=255)
 aGGTTTGGCCGCTGCAGGCTTGGCTGCTGGCTTAGCTGCGGGTTTTTCCGCTGCGGTTTTGGCTGCGGGTTTGGCCGCTGCGGTTTTGCCAGCAGGTTTG‑‑‑GCCGCCGCCGTTTTCGCCGCAGGCTTAGCTGCTGCCTTGGCCAGTGGCtt                                                                                                                                          <  1:672380/150‑1 (MQ=255)
        gCCGCTGCAGGCTTGGCTGCTGGCTTAGCTGCAGGTTTTTCCGCTGCGGTTTTGGCTGCGGGTTTGGCCGCTGCGGTTTTGCCAGCAGGTTTG‑‑‑GCCGCCGCCGTTTTCGCCGCAGGCTTAGCTGCTGCCTTGGCCAGTGGCTTGGCCGCa                                                                                                                                   >  1:382246/1‑150 (MQ=255)
               cAGGCTTGGCTGCTGGCTTAGCTGCAGGTTTTTCCGCTGCGGTTTTGGCTGCGGGTTTGGCCGCTGCGGTTTTGCCAGCAGGTTTG‑‑‑GCCGCCGCCGTTTTCGCCGCAGGCTTAGCTGCTGCCTTGGCCAGTGGCTTGGCCGCAGCcttgc                                                                                                                            <  1:709201/150‑1 (MQ=255)
               cAGGCTTGGCTGCTGGCTTAGCTGCAGGTTTTTCCGCTGCGGTTTTGGCTGCGGGTTTGGCCGCTGCGGTTTTGCCAGCAGGTTTG‑‑‑GCCGCCGCCGTTTTCGCCGCAGGCTTAGCTGCTGCCTTGGCCAGTGACTTGGCCGCAGCcttgc                                                                                                                            >  1:493626/1‑150 (MQ=255)
               cAGGCTTGGCTGCTGGCTTAGCTGCAGGTTTTTCCGCTGCGGTTTTGGCTGCGGGTTTGGCCGCTGCGGTTTTGCCAGCAGGTTTG‑‑‑GCCGCCGCCGTTTTCGCCGCAGGCTTAGCTGCTGCCTTGGCCAGTGACTTGGCCGCAGCcttgc                                                                                                                            >  1:493624/1‑150 (MQ=255)
                                     tGCAGGTTTTTCCGCTGCGGTTTTGGCTGCGGGTTTGGCCGCTGCGGTTTTGCCAGCAGGTTTG‑‑‑GCCGCCGCCGTTTTCGCCGCAGGCTTAGCTGCTGCCTTGGCCAGTGGCtt                                                                                                                                          >  1:312300/1‑114 (MQ=255)
                                     tGCAGGTTTTTCCGCTGCGGTTTTGGCTGCGGGTTTGGCCGCTGCGGTTTTGCCAGCAGGTTTG‑‑‑GCCGCCGCCGTTTTCGCCGCAGGCTTAGCTGCTGCCTTGGCCAGTGGCtt                                                                                                                                          <  2:312300/114‑1 (MQ=255)
                                      gcgggTTTTTCCGCTGCGGTTTGGGCTGCGGGCTTGGCCGCTGCGGTTTTGCTAGCAGGTTTGGCCGCCGCCGCCGTTTTCGCCGCAGGCTTAGCTGCTGCCTTGGCCAGTGGCTTGGCCGCAGCCTTGCTTGCAGCCGGCTTGGTtgct                                                                                                        <  2:30609/147‑1 (MQ=255)
                                      gCAGGTTTTTCCGCTGCGGTTTTGGCTGCGGGTTTGGCCGCTGCGGTTTTGCCAGCAGGTTTG‑‑‑GCCGCCGCCGTTTTCGCCGCAGGCTTAGCTGCTGCCTTGGCCAGTGGCTTGGCCGCAGCCTTGCTTGCAGCCGGCTTGGTTGGNGCa                                                                                                     >  1:817823/1‑150 (MQ=255)
                                       aaGGTTTTTCCGCTGCGTGTTTGGCTGCGGGTTTGGCCGCTGCGGTTTTGCCAGCAGGTTTG‑‑‑GCCGCCGCCGTTTTCGCCGCAGGCTTAGCTGCTGCCTTGGCCAGTGGCTTGGCCGCAGCCTTGCTTGCAGCCGGCTTGGTTGCTGCAg                                                                                                    >  2:244169/2‑150 (MQ=255)
                                          gTTTTTCCGCTGCGGTTTTGGCTGCGGGTTTGGCCGCTGCGGTTTTGCCAGCAGGTTTG‑‑‑GCCGCCGCCGTTTTCGCCGCAGGCTTAGCTGCTGCCTTGGCCAGTGGCTTGGCCGCAGCCTTGCTTGCAGCCGGCTTGGTTGCTGCAgcgc                                                                                                 >  2:300915/1‑150 (MQ=255)
                                                        gTTTTGGCTGCGGTTTTGCCCGCTGCGGTTTTGCGTGCAGGCTTG‑‑‑GGCTCTTCCGATCTCACCGCTATCTGTGTTGCTGCCTTGGCCAGTGGCTTGGCCGCAGCCTTGCTTGCAGTCGACTTGGTTTCTGCAGCGCGCGACGAAATCGGc                                                                                   <  1:406926/150‑1 (MQ=255)
                                                        gTTTTGGCTGCGGGTTTGGCCGCTGCGGTTTTGCCAGCAGGTTTG‑‑‑GCCGCCGCCGTTTTCGCCGCAGGCTTAGCTGCTGCCTTGGCCAGTGGCTTGGCCGCAGCCTTGCTTGCAGCCGGCTTGGTTGCTGCAGCGCGCGACGAAATCGGc                                                                                   <  2:603005/150‑1 (MQ=255)
                                                        gTTTTGGCTGCGGGTTTGGCCGCTGCGGTTTTGCCAGCAGGTTTG‑‑‑GCCGCCGCCGTTTTCGCCGCAGGCTTAGCTGCTGCCTTGGCCAGTGGCTTGGCCGCAGCCTTGCTTGCAGCCGGCTTGGTTGCTGCAGCGCGCGACGAAATCGGc                                                                                   <  2:96790/150‑1 (MQ=255)
                                                        gTTTTGGCTGCGGGTTTGGCCGCTGCGGTTTTGCCAGCAGGTTTG‑‑‑GCCGCCGCCGTTTTCGCCGCAGGCTTAGCTGCTGCCTTGGCCAGTGGCTTGGCCGCAGCCTTGCTTGCAGCCGGCTTGGTTGCTGCAGCGCGCGACGAAATCGGc                                                                                   <  1:810874/150‑1 (MQ=255)
                                                             ggCTGCGGGTTTGGCCGCTGCGGTTTTGCCAGCAGGTTTG‑‑‑GCCGCCGCCGTTTTCGCCGCAGGCTTAGCTGCTGCCTTGGCCAGTGGCTTGGCCGCAGCctt                                                                                                                              <  2:123358/102‑1 (MQ=255)
                                                             ggCTGCGGGTTTGGCCGCTGCGGTTTTGCCAGCAGGTTTG‑‑‑GCCGCCGCCGTTTTCGCCGCAGGCTTAGCTGCTGCCTTGGCCAGTGGCTTGGCCGCAGCctt                                                                                                                              >  1:123358/1‑102 (MQ=255)
                                                                     gTTTGGCCGCTGCGGTTTTGCCAGCAGGTTTG‑‑‑GCCGCCGCCGTTTTCGCCGCAGGCTTAGCTGCTGCCTTGGCCAGTGGCTTGGCCGCAGCCTTGCTTGCAGCCGGCTTGGTTGCTGCAGCGCGCGACGAAATCGGCGTAACCGAAGCAc                                                                      >  2:260569/1‑150 (MQ=255)
                                                                       ttGGCCGCTGCGGTTTTGCCAGCAGGTTTG‑‑‑GCCGCCGCCGTTTTCGCCGCAGGCTTAGCTGCTGCCTTGGCCAGTGGCTTGGCCGCAGCCTTGCTTGCAGCCGGCTTGGTTGCTGCAGCGCGCGACGAAATCGGCGTAACCGAAGCACCg                                                                    >  1:854879/1‑150 (MQ=255)
                                                                          gCCGCTGCGGTTTTGCCAGCAGGTTTG‑‑‑GCCGCCACCGTTTTCGCCGCAGGCTTAGCTGCTGCCTTGGCCAGTGGCTTGGCCGCAGCCTTGCTTGCAGCCGGCTTGGTTGCTGCAGCGCGCGACGAAATCg                                                                                     >  1:1045556/1‑130 (MQ=255)
                                                                          gCCGCTGCGGTTTTGCCAGCAGGTTTG‑‑‑GCCGCCACCGTTTTCGCCGCAGGCTTAGCTGCTGCCTTGGCCAGTGGCTTGGCCGCAGCCTTGCTTGCAGCCGGCTTGGTTGCTGCAGCGCGCGACGAAATCg                                                                                     <  2:1045556/130‑1 (MQ=255)
                                                                               tGCGGTTTTGCCAGCAGGTTTG‑‑‑GCCGCCGCCGTTTTCGCCGCAGGCTTAGCTGCTGCCTTGGCCAGTGGCTTGGCCGCAGCCTTGCTTGCAGCCGGCTTGGTTGCTGCAGCGCGCGACGAAATCGGCGTAACCGAAGCACCGGTCAGCtt                                                            >  2:21643/1‑150 (MQ=255)
                                                                                          cagcagGTTTG‑‑‑GCCGCCGCCGTTTTCGCCGCAGGCTTAGCTGCTGCCTTGGCCAGTGGCTTGGCCGCAGCCTTGCTTGCAGCCGGCTTGGTTGCTGCAGCGCGCGACGAAATCGGCGTAACCGACGCACCGGTCAGCTTCTCGCTATGCt                                                 <  2:1034335/150‑1 (MQ=255)
                                                                                          cagcagGTTTG‑‑‑GCCGCCGCCGTTTTCGCCGCAGGCTTAGCTGCTGCCTTGGCCAGTGGCTTGGCCGCAGCCTTGCTTGCAGCCGGCTTGGTTGCTGCAGCGCGCGACGAAATCGGCGTAACCGAAGCACCGGTCAGCTTCTCGATCTGCt                                                 >  1:111649/1‑150 (MQ=255)
                                                                                           agcagGTTTG‑‑‑GCCGCCGCCGTTTTCGCCGCAGGCTTAGCTGCTGCCTTGGCCAGTGGCTTGGCCGCAGCCTTGCTTGCAGCCGGCTTGGTTGCTGCAGCGCGCGACGAAATCGGCGTAACCGAAGCACCGGTCAGCTTCTCGATCTGCtt                                                >  1:122623/1‑150 (MQ=255)
                                                                                           agcagGTTTG‑‑‑GCCGCCGCCGTTTTCGCCGCAGGCTTAGCTGCTGCCTTGGCCAGTGGCTTGGCCGCAGCCTTGCTTGCAGCCGGCTTGGTTGCTGCAGCGCGCGACGAAATCGGCGTAACCGAAGCACCGGTCAGCTTCTCGATCTGCtt                                                <  2:260928/150‑1 (MQ=255)
                                                                                            gcagGTTTG‑‑‑GCCGCCGCCGTTTTCGCCGCAGGCTTAGCTGCTGCCTTGGCCAGTGGCTTGGCCGCAGCCTTGCTTGCAGCCGGCTTGGTTGCTGCAGCGCGCGACGAAATCGGCGTAACCGAAGCACCGGTCAGCTTCTCGATCTGCTTg                                               >  1:315716/1‑150 (MQ=255)
                                                                                                 tttG‑‑‑GCCGCCGCCGTTTTCGCCGCAGGCTTAGCTGCTGCCTTGGCCAGTGGCTTGGCCGCAGCCTTGCTTGCAGCCGGCTTGGTTGCTGCAGCGCGCGACGAAATCGGCGTAACCGAAGCACCGGTCAGCTTCTCGATCTGCTTGGTCAg                                          <  2:122623/150‑1 (MQ=255)
                                                                                                  ttG‑‑‑GCCGCCGCCGTTTTCGCCGCAGGCTTAGCTGCTGCCTTGGCCAGTGACTTGGCCGCAGCCTTGCTTGCAGCCGGCTTGGTTGCTGCAGCGCGCGACGAAATCGGCGTAACCGAAGCACCGGTCAGCTTCTCGATCTGCTTGGTCAgg                                         <  2:493626/150‑1 (MQ=255)
                                                                                                  ttG‑‑‑GCCGCCGCCGTTTTCGCCGCAGGCTTAGCTGCTGCCTTGGCCAGTGACTTGGCCGCAGCCTTGCTTGCAGCCGGCTTGGTTGCTGCAGCGCGCGACGAAATCGGCGTAACCGAAGCACCGGTCAGCTTCTCGATCTGCTTGGTCAgg                                         <  2:493624/150‑1 (MQ=255)
                                                                                                             cgccgTTTTCGCCGCAGGCTTAGCTGCTGCCTTGGCCAGTGGCTTGGCCGCAGCCTTGCTTGCAGCCGGCTTGGTTGCTGCAGCGCGCGACGAAATCGGCGTAACCGAAGCACCGGTCAGCTTCTCGATCTGCTTGGTCAGGCTGTCCAc                                 >  1:1092626/1‑150 (MQ=255)
                                                                                                              gccgTTTTCGCCGCAGGCTTAGCTGCTGCCTTGGCCAGTGGCTTGGCCGCAGCCTTGCTTGCAGCCGGCTTGGTTGCTGCAGCGCGCGAAGAAACCATACCTGCCTAATCTCCGGTCAGCTTCTCGATCTGCTTGGTCAGGCTGTCCAcc                                <  1:300915/150‑1 (MQ=255)
                                                                                                                 gTTTTCGCCGCAGGCTTAGCTGCTGCCTTGGCCAGTGGCTTGGCCGCAGCCTTGCTTGCAGCCGGCTTGGTTGCTGCAGCGCGCGACGAAATCGGCGTAACCGAAGCACCGGTCAGCTTCTCGATCTGCTTGGTCAGGCTGTCCACctgc                             <  1:203727/150‑1 (MQ=255)
                                                                                                                        ccgcAGGCTTAGCTGCTGCCTTGGCCAGTGGCTTGGCCGCAGCCTTGCTTGCAGCCGGCTTGGTTGCTGCAGCGCGCGACGAAATCGGCGTAACCGAAGCACCGGTCAGCTTCTCGATCTGCTTGGTCAGGCTGTCCACCTGCTGGTGCa                      <  2:163717/150‑1 (MQ=255)
                                                                                                                        ccgcAGGCTTAGCTGCTGCCTTGGCCAGTGGCTTGGCCGCAGCCTTGCTTGCAGCCGGCTTGGTTGCTGCAGCGCGCGACGAAATCGACGTAACCGAAGCACCGGTCAGCTTCTCGATCTGCTTGGTCAGGCTGTCCACCTGCTGGTGCa                      <  2:765216/150‑1 (MQ=255)
                                                                                                                            aGGCTTAGCTGCTGCCTTGGCCAGTGGCTTGGCCGCAGCCTTGCTTGCAGCCGGCTTGGTTGCTGCAGCGCGCGACGAAATCGGCGTAACCGAAGCACCGGTCAGCTTCTCGATCTGCTTGGTCAGGCTGTCCACCTGCTGGTGCAGTGc                  <  2:80996/150‑1 (MQ=255)
                                                                                                                             ggCTTAGCTGCTGCCTTGGCCAGTGGCTTGGCCGCAGCCTTGCTTGCAGCCGGCTTGGTTGCTGCAGTTCGCGACGAAATCGGCGTAACCGAAGCACCGGTCAGCTTCTCGATCTGCTTGGTCAGGCTGTCCACCTGCTGGTGCAGTGcc                 <  1:244169/150‑1 (MQ=255)
                                                                                                                              gCTTAGCTGCTGCCTTGGCCAGTGGCTTGGCCGCAGCCTTGCTTGCAGCCGGCTTGGTTGCTGCAGCGCGCGACGAAATCGGCGTAACCGAAGCACCGGTCAGCTTCTCGATCTGCTTGGTCAGGCTGTCCACCTGCTGGTGCAGTGCCt                >  2:802314/1‑150 (MQ=255)
                                                                                                                                      gctgcCTTGGCCAGTGGCTTGGCCGCAGCCTTGCTTGCAGCCGGCTTGGTTGCTGCAGCGCGCGACGAAATCGGAGTAAACGACGCCCCGGTCAAGAACTCGATCTGCTAGTTCAGGCTGTCCACCTGCTGGTGCAGTGCCTTGATCTCg        <  1:21643/150‑1 (MQ=255)
                                                                                                                                          ccTTGGCCAGTGGCTTGGCCGCAGCCTTGCTTGCAGCCGGCTTGGTTGCTGCAGCGCGCGACGAAATCGGCGTAACCGAAGCACCGGTCAGCTTCTCGATCTGCTTGGTCAGGCTGTCCACCTGCTGGTGCAGTGCCtt               >  2:1091753/1‑139 (MQ=255)
                                                                                                                                          ccTTGGCCAGTGGCTTGGCCGCAGCCTTGCTTGCAGCCGGCTTGGTTGCTGCAGCGCGCGACGAAATCGGCGTAACCGAAGCACCGGTCAGCTTCTCGATCTGCTTGGTCAGGCTGTCCACCTGCTGGTGCAGTGCCtt               <  1:1091753/139‑1 (MQ=255)
                                                                                                                                          ccTTGGCCAGTGGCTTGGCCGCAGCCTTGCTTGCAGCCGGCTTGGTTGCTGCAGCGCGCGACGAAATCGGCGTAACCGAAGCACCGGTCAGCTTCTCGATCTGCTTGGTCAGGCTGTCCACCTGCTGGTGCAGTGCCTTGATCTCGTTGc    >  2:14200/1‑150 (MQ=255)
                                                                                                                                            ttGGCCAGTGGCTTGGCCGCAGCCTTGCTTGCAGCCGGCTTGGTTGCTGCAGCGCGCGACGAAATCGGCGTAACCGAAGCACCGGTCAGCTTCTCGATCTGCTTGGTCAGGCTGTCCACCTGCTGGTGCAGTGCCTTGATCTCGTTGCgg  <  2:382246/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                           
CAGGTTTGGCCGCTGCAGGCTTGGCTGCTGGCTTAGCTGCAGGTTTTTCCGCTGCGGTTTTGGCTGCGGGTTTGGCCGCTGCGGTTTTGCCAGCAGGTTTG‑‑‑GCCGCCGCCGTTTTCGCCGCAGGCTTAGCTGCTGCCTTGGCCAGTGGCTTGGCCGCAGCCTTGCTTGCAGCCGGCTTGGTTGCTGCAGCGCGCGACGAAATCGGCGTAACCGAAGCACCGGTCAGCTTCTCGATCTGCTTGGTCAGGCTGTCCACCTGCTGGTGCAGTGCCTTGATCTCGTTGCGG  >  NC_002947/5704813‑5705099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑