Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,796,178 C→T 7.7% R593H (CGC→CAC)  gltB ← L‑glutamate synthase subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,796,1780CT 7.7% 145.9 / 2.6 52R593H (CGC→CAC) gltBL‑glutamate synthase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (21/27);  new base T (2/2);  total (23/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

GCCCTGTTCGGTCAGGCGGTGGTGCACTGCACCAACGGCCAGCGAAGCGTGCACCGGCAGCTTGCCCGGGGCGATATAACGGTCGCTCAGCACCAGCTGGGTCTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATGGCCGCTTCCAGGCCGACGCTCTGCTCATAGTTGAGGTCGATCAGCTGGCGGTCGAAGCCTTCGCGCTCCAGGTTCATCAGCGAACGCCACTTGGCGGGCGAGATGACCGGCGAGCTGAGGATGACCCGCGAAGCGTGCTCCGG  >  NC_002947/5796033‑5796326
                                                                                                                                                 |                                                                                                                                                    
gCCCTGTTCGGTCAGGCGGTGGTGCACTGCACCAACGGCCAGCGAAGCGTGCACCGGCAGCTTGCCCGGGGCGATATAACGGTCGCTCAGCACCAGCTGGGTCTTGCCCGTTTTCACAGCTTCTTCGGCCTGGTCGGCAATGTTGCGGAt                                                                                                                                                  >  1:336382/1‑150 (MQ=255)
 cccTGTTCGGTCAGGCGGTGGTGCACTGCACCAACGGCCAGCGAAGCGTGCACCGGCAGCTTGCCCGGGGCGATATAACGGTCGCTCAGCACCAGCTGGGTCTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATg                                                                                                                                                 <  2:251943/150‑1 (MQ=255)
  ccTGTTCGGTTAGGCGGTGGTGCACTGCACCAACGGCCAGCGAAGCGTACACCAGAAGTGTGCTCTTGTGTTTATAACGGTCGCTCAGCACCAGCTGGGTCTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATgg                                                                                                                                                <  1:976595/150‑1 (MQ=255)
  ccTGTTCGGTCAGGCGGTGGTGCACTGCACCAACGGCCAGCGAAGCGTGCACCGGCAGCTTGCCCGGGGCGATATAACGGTCGCTCAGCACCAGCTGGGTCTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATgg                                                                                                                                                >  2:289265/1‑150 (MQ=255)
           tCAGGCGGTGGTGCACTGCACCAACGGCCAGCGAAGCGTGCACCGGCAGCTTGCCCGGGGCGATATAACGGTCGCTCAGCACCAGCTGGGTCTTGTCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATGGCCGCTTCCa                                                                                                                                       <  1:1059675/150‑1 (MQ=255)
               gCGGTGGTGCACTGCACCAACGGCCAGCGAAGCGTGCACCGGCAGCTTGCCCGGGGCGATATAACGGTCGCTCAGCACCAGCTGGGTCTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATGGCCGCTTCCAGGcc                                                                                                                                   <  2:109116/150‑1 (MQ=255)
                  gtggtgCACTGCACCAACGGCCAGCGAAGCGTGCACCGGCAGCTTGCCCGGGGCGATATAACGGTCGCTCAGCACCAGCTGGGTCTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATGGCCGCTTCCAGGCCGAc                                                                                                                                >  2:70756/1‑150 (MQ=255)
                         actgcacCAACGGCCAGCGAAGCGTGCACCGGCAGCTTGCCCGGGGCGATATAACGGTCGCTCAGCACCAGCTGGGTCTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATGGCCGCTTCCAGGCCGACGCTCTGc                                                                                                                         <  2:400261/150‑1 (MQ=255)
                         actgcacCAACGGCCAGCGAAGCGTGCACCGGCAGCTTGCCCGGGGCGATATAACGGTCGCTCAGCACCAGCTGGGTCTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATGGCCGCTTCCAGGCCGACGCTCTGc                                                                                                                         >  1:400261/1‑150 (MQ=255)
                              acCAACGGCCAGCGAAGCGTGCACCGGCAGCTTGCCCGGGGCGATATAACGGTCGCTCAGCACCAGCTGGGTCTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATGGCCGCTTCCAGGCCGACGCTCTg                                                                                                                          <  2:931544/144‑1 (MQ=255)
                              acCAACGGCCAGCGAAGCGTGCACCGGCAGCTTGCCCGGGGCGATATAACGGTCGCTCAGCACCAGCTGGGTCTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATGGCCGCTTCCAGGCCGACGCTCTg                                                                                                                          >  1:931544/1‑144 (MQ=255)
                               ccAACGGCCAGCGAAGCGTGCACCGGCAGCTTGCCCGGGGCGATATAACGGTCGCTCAGCACCAGCTGGGTCTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATGGCCGCTTCCAGGCCGACGCTCTGCTCATAg                                                                                                                   <  2:672479/150‑1 (MQ=255)
                                cAACGGCCAGCGAAGCGTGCACCGGCAGCTTGCCCGGGGCGATATAACGGTCGCTCAGCACCAGCTGGGTCTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATGGCCGCTTCCAGGCCGACGCTCTGCTCATAGt                                                                                                                  >  2:772336/1‑150 (MQ=255)
                                cAACGGCCAGCGAAGCGTGCACCGGCAGCTTGCCCGGGGCGATATAACGGTCGCTCAGCACCAGCTGGGTCTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATGGCCGCTTCCAGGCCGACGCTCTGCTCATAGt                                                                                                                  >  1:639115/1‑150 (MQ=255)
                                   cGGCCAGCGAAGCGTGCACCGGCAGCTTGCCCGGGGCGATATAACGGTCGCTCAGCACCAGCTGGGTCTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATGGCCGCTTCCAGGCCGACGCTCTGCTCATAGTTGa                                                                                                               <  1:233303/150‑1 (MQ=255)
                                      ccAGCGAAGCGTGCACCGGCAGCTTGCCCGGGGCGATATAACGGTCGCTCAGCACCAGCTGGGTCTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATGGCCGCTTCCAGGCCGACGCTCTGCTCACAGTTGAgtt                                                                                                            <  2:801086/150‑3 (MQ=255)
                                       cAGCGAAGCGTGCACCGGCAGCTTGCCCGGGGCGATATAACGGTCGCTCAGCACCAGCTGGGTCTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGTGGATGGCCGCTTCCAGGCCGACGCTCTGCTCATAGTTGAGGTc                                                                                                           >  2:788124/1‑150 (MQ=255)
                                       cAGCGAAGCGTGCACCGGCAGCTTGCCCGGGGCGATATAACGGTCGCTCAGCACCAGCTGGGTCTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGTGGATGGCCGCTTCCAGGCCGACGCTCTGCTCATAGTTGAGGTc                                                                                                           >  2:788125/1‑150 (MQ=255)
                                       cAGCGAAGCGTGCACCGGCAGCTTGCCCGGGGCGATATAACGGTCGCTCAGCACCAGCTGGGTCTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGAAAGGGTGCGGATGGCCGCTTCCAGGCCGACGCTCTGCTCATAGTTGAGGTc                                                                                                           >  1:68055/1‑150 (MQ=255)
                                          cGAAGCGTGCACCGGCAGCTTGCCCGGGGCGATATAACGGTCGCTCAGCACCAGCTGGGTCTTGCCCCCACGCACAGCTCCGTCGGCCTGGTCGGCAATGTGGCGGATGGCCGCATCCAGACATACGCTCTGCTCATAGTTGAGGTCGAt                                                                                                        >  1:299223/1‑150 (MQ=255)
                                           gAAGCGTGCACCGGCAGCTTGCCTGGGGCGATATAACGGTCGCTCAGCACCAGCTGGGTCTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATGGCCGCTTCCAGGCCGACGCTCTGCTCATAGTTGAGGTCGATc                                                                                                       >  2:552290/1‑150 (MQ=255)
                                              gCGTGCACCGGCAGCTTGCCCGGGGCGATATAACGGTCGCTCAGCGTTAGCTGGGTCTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATGGCCGCTTCCAGGCCGACGCTCTGCTCATAGTTGAGGTCGATCAGc                                                                                                    <  1:674270/150‑1 (MQ=255)
                                               cGTGCACCGGCAGCTTGCCCGGGGCGATATAACGGTCGCTCAGCACCAGCTGGGTCTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATGGCCGCTTCCAGGCCGACGCTCTGCTCATAGTTGAGGTCGATCAGCt                                                                                                   <  2:379840/150‑1 (MQ=255)
                                                   cACCGGCAGCTTGCCCGGGGCGATATAACGGTCGCTCAGCACCAGCTGGGTCTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATGGCCGCTTCCAGGCCGACGCTCTGCTCATAGTTGAGGTCGATCAGCTGGCg                                                                                               >  1:799579/1‑150 (MQ=255)
                                                   cACCGGCAGCTTGCCCGGGGCGATATAACGGTCGCTCAGCACCAGCTGGGTCTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATGGCCGCTTCCAGGCCGACGCTCTGCTCATAGTTGAGGTCGATCAGCTGGCg                                                                                               >  1:918634/1‑150 (MQ=255)
                                                      cGGCATCGTGCCCGGGGCGATATAACGGTCGCTCAGCACCAGCTGGGTCTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATGGCCGCTTCCAGGCCGACGCTCTGCTCATAGTTGAGGTCGATCAGCTGGCGGTc                                                                                            <  2:279807/150‑1 (MQ=255)
                                                        gCAGCTTGCCCGGGGCGATATAACGGTCGCTCAGCACCAGCTGGGTCTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATGGCCGCTTCCAGGCCGACGCTCTGCTCATAGTTGa                                                                                                               <  1:257756/129‑1 (MQ=255)
                                                        gCAGCTTGCCCGGGGCGATATAACGGTCGCTCAGCACCAGCTGGGTCTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATGGCCGCTTCCAGGCCGACGCTCTGCTCATAGTTGa                                                                                                               >  2:257756/1‑129 (MQ=255)
                                                         cAGCTTGCCCGGGGCGATATAACGGTCGCTCAGCACCAGCTGGGTCTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATGGCCGCTTCCAGGCCGACGCTCTGCTCATAGTTGAGGTCGATCAGCTGGCGGTCGaa                                                                                         <  1:70756/150‑1 (MQ=255)
                                                               gCCCGGGGCGATATAACGGTCGCTCAGCACCAGCTGGGTCTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATGGCCGCTTCCAGGCCGACGCTCTGCTCATAGTTGAGGTCGATCAGCTGGCGGTCGAAGCCTTc                                                                                   <  2:918634/150‑1 (MQ=255)
                                                                cccGGGGCGATATAACGGTCGCTCAGCACCAGCTGGGTCTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATGGCCGCTTCCAGGCCGACGCTCTGCTCATAGTTGAGGTCGATCAGCTGGCGGTCGAAGCCTTcg                                                                                  <  1:471925/150‑1 (MQ=255)
                                                                 ccGGGGCGATATAACGGTCGCTCAGCACCAGCTGGGTCTTGACCCCACGCCCAGCTTCTTCGGCCTGGTCGGCAATGTTTCGGATGGTCTCTTTCAGGCCGACGCTCTGCTCATAGTTGAGGTCGATCAGCTGGCGGTCGAAGCCTTcgc                                                                                 >  2:971521/1‑150 (MQ=255)
                                                                         atatAACGGTCGCTCAGCACCAGCTGGGTCTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATGGCCGCTTCCAGGCCGACGCTCTGCTCATAGTTGAGGTCGATCAGCTGGCGGTCGAAGCCTTCGCGCTCCAgg                                                                         >  2:517795/1‑150 (MQ=255)
                                                                          tataACGGTCGCTCAGCACCAGCTGGGTCTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATGGCCGCTTCCAGGCCGACGCTCTGCTCATAGTTGAGGTCGATCAGCTGGCGGTCGAAGCCTTCGCGCTCCAGGt                                                                        <  2:1106273/150‑1 (MQ=255)
                                                                                    gCTCAGCACCAGCTGGGTCTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATGGCCGCTTCCAGGCCGACGCTCTGCTCATAGTTGAGGTCGATCAGCTGGCGGTCGAAGCCTTCGCGCTCCAGGTTCATCAGCGa                                                              >  2:469707/1‑150 (MQ=255)
                                                                                      tCAGCACCAGCTGGGTCTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATGGCCGCTTCCa                                                                                                                                       >  2:535020/1‑75 (MQ=255)
                                                                                      tCAGCACCAGCTGGGTCTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATGGCCGCTTCCa                                                                                                                                       <  1:535020/75‑1 (MQ=255)
                                                                                        aGCACCAGCTGGGTCTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATGGCCGCTTCCAGGCCGACGCTCTGCTCATAGTTGAGGTCGATCAGCTGGCGGTCGAAGCCTTCGCGCTCCAGGTTCATCAGCGAACGc                                                          <  2:979056/150‑1 (MQ=255)
                                                                                          cACCAGCTGGGTCTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATGGCCGCTTCCAGGCCGAc                                                                                                                                <  2:698964/78‑1 (MQ=255)
                                                                                          cACCAGCTGGGTCTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATGGCCGCTTCCAGGCCGAc                                                                                                                                >  1:698964/1‑78 (MQ=255)
                                                                                                  gggTCTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATGGCCGCTTCCAGGCCGACGCTCTGCTCATAGTTGAGGTCGATCAGCTGGCGGTCGAAGCCTTCGCGCTCCAGGTTCATCAGCGAACGCCACTTggcgg                                                <  2:639115/150‑1 (MQ=255)
                                                                                                      cTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATGGCCGCTTCCAGGCCGACGCTCTGCTCATAGTTGAGGTCGATCAGCTGGCGGTCGAAGCCTTCGCGCTCCAGGTTCATCAGCGAACGCCACTTGGCGGGCga                                            <  1:351296/150‑1 (MQ=255)
                                                                                                        tGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGTGGATGGCCGCTTCCAGGCCGACGCTCTGCTCATAGTTGAGGTCGATCAGCTGGCGGTCGAAGCCTTCGCGCTCCAGGTTCATCAGCGAACGCCACTTGGCGGGCgaga                                          <  1:788125/150‑1 (MQ=255)
                                                                                                        tGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGTGGATGGCCGCTTCCAGGCCGACGCTCTGCTCATAGTTGAGGTCGATCAGCTGGCGGTCGAAGCCTTCGCGCTCCAGGTTCATCAGCGAACGCCACTTGGCGGGCgaga                                          <  1:788124/150‑1 (MQ=255)
                                                                                                                   acaGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATGGCCGCTTCCAGGCCGACGCTCTGCTCATAGTTGAGGTCGATCAGCTGGCGGTCGAAGCCTTCGCGCTCCAGGTTCATCAGCGAACGCCACTTGGCGGGCGAGATGACCGGCGAg                               >  2:962125/1‑150 (MQ=255)
                                                                                                                     aGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATGGCCGCTTCCAGGCCGACGCTCTGCTCATAGTTGAGGTCGATCAGCTGGCGGTCGAAGCCTTCGCGCTGCAGGTTCATCAGCGAACGCCACTTGGCGGGCGAGATGACCGGCGAGCt                             >  1:291763/1‑150 (MQ=255)
                                                                                                                        ttcttcGGCCTGGTGGGCAATGTTGCGGATGGCCGCTTACAGGCCGACTCTCTGCTCATAGTTGAGGTCGATCAGCTGGCGGTCGAAGCCTTCGCGCTCCAGGTTCATCAGCGAACGCCACTTGGCGGGCGAGATGACCGGCGAGCTGAg                          <  2:68055/150‑1 (MQ=255)
                                                                                                                                   ggTCGGCAATGTTGCGGATGGCCGCTTCCAGGCCGACGCTCTGCTCATAGTTGAGGTCGATCAGCTGGCGGTCGAAGCCTTc                                                                                   <  2:253487/82‑1 (MQ=255)
                                                                                                                                   ggTCGGCAATGTTGCGGATGGCCGCTTCCAGGCCGACGCTCTGCTCATAGTTGAGGTCGATCAGCTGGCGGTCGAAGCCTTc                                                                                   >  1:253487/1‑82 (MQ=255)
                                                                                                                                     tCGGCAATGTTGCGGATGGCCGCTTCCAGGCCGACGCTCTGCTCATAGTTGAGGTCGATCAGCTGGCGGTCGAAGCCTTCGCGCTCCAGGTTCATCAGCGAACGCCACTTGGCGGGCGAGATGACCGGCGAGCTGAGGATGACCCGCGaa             <  1:289265/150‑1 (MQ=255)
                                                                                                                                       ggCAATGTTGCGGATGGCCGCTTCCAGGCCGACGCTCTGCTCATAGTTGAGGTCGATCAGCTGGCGGTCGAAGCCTTCGCGCTCCAGGTTCATCAGCGAACGCCACTTGGCGGGCGAGATGACCGGCGAGCTGAGGATGACCCGCGAAGc           <  1:263031/150‑1 (MQ=255)
                                                                                                                                                gCGGATGGCCGCTTCCAGGCCGACGCTCTGCTCATAGTTGAGGTCGATCAGCTGGCGGTCGAAGCCTTCGCGCTCCAGGTTCATCAGCGAACGACACTTGGCGGGCGAGCTGACCGGTGAGCTGATTCTGCCCCGCGAAGCTTGCTCCgg  <  2:799579/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GCCCTGTTCGGTCAGGCGGTGGTGCACTGCACCAACGGCCAGCGAAGCGTGCACCGGCAGCTTGCCCGGGGCGATATAACGGTCGCTCAGCACCAGCTGGGTCTTGCCCCCACGCACAGCTTCTTCGGCCTGGTCGGCAATGTTGCGGATGGCCGCTTCCAGGCCGACGCTCTGCTCATAGTTGAGGTCGATCAGCTGGCGGTCGAAGCCTTCGCGCTCCAGGTTCATCAGCGAACGCCACTTGGCGGGCGAGATGACCGGCGAGCTGAGGATGACCCGCGAAGCGTGCTCCGG  >  NC_002947/5796033‑5796326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑