Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 493,102 G→A 8.7% Q183Q (CAG→CAA)  PP_0405 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947493,1020GA 8.7% 125.6 / 3.4 46Q183Q (CAG→CAA) PP_0405hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (22/20);  new base A (2/2);  total (24/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01

TTTGCCAATGGACGCGCCGCTGCCCAGCTACGACGACGCCCTGCTGCGCCGTGAAGTGGAACTGTTCCCCGAATGGTACGTGGGGCGTGAGCTGGGCCTGACCTTCACCGATGCACAGAAGGCCACCTGGCAGCGCGTCAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGACATTACCTGCCT  >  NC_002947/492958‑493251
                                                                                                                                                |                                                                                                                                                     
tttGCCAATGGACGCGCCGCTGCCCAGCTACGACGACGCCCTGCTGCGCCGTGAAGTGGAACTGTTCCCCGAATGGTACGTTGGGCGCGAGCTGGGCCTGACCTTCACCGATGCACAGAAGGCCACCTGGCAGCGCGTCAGCCAGTTGCt                                                                                                                                                  >  2:1563/1‑150 (MQ=255)
       aTGGACGCGCCGCTGCCCAGCTACGACGACGCCCTGCTGCGCCGTGAAGTGGAACTGTTCCCCGAATGGTACGTGGGGCGTGAGCTGGGCCTGACCTTCACCGATGCACAGAAGGCCACCTGGCAGCGCGTCAGCCAGTTGCTGATCGAc                                                                                                                                           <  2:330651/150‑1 (MQ=255)
           aCGCGCCGCTGCCCAGCTACGACGACGCCCTGCTGCGCCGTGAAGTGGAACTGTTCCCCGAATGGTACGTGGGGCGTGAGCTGGGCCTGACCTTCACCGATGCACAGAAGGCCACCTGGCAGCGCGTCAGCCAGTTGCTGATCGACAgcg                                                                                                                                       >  1:194121/1‑150 (MQ=255)
                 cgcTGCCCAGCTACGACGACGCCCTGCTGCGCCGTGAAGTGGAACTGTTCCCCGAATGGTACGTGGGGCGTGAGCTGGGCCTGACCTTCACCGATGCACAGAAGGCCACCTGGCAGCGCGTCAGCCAGTTGCTGATCGACAGCGCCCTgg                                                                                                                                 >  1:877579/1‑150 (MQ=255)
                       ccAGCTACGACGACGCCCTGCTGCGCCGTGAAGTGGAACTGTTCCCCGAATGGTACGTGGGGCGTGAGCTGGGCCTGACCTTCACCGATGCACAGAAGGCCACCTGGCAGCGCGTCAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGc                                                                                                                           >  1:170725/1‑150 (MQ=255)
                       ccAGCTACGACGACGCCCTGCTGCGCCGTGAAGTGGAACTGTTCCCCGAATGGTACGTGGGGCGTGAGCTGGGCCTGACCTTCACCGATGCACAGAAGGCCACCTGGCAGCGCGTCAGCCAATTGCTGATCGACAGCGCCCTGGCGCAGc                                                                                                                           >  2:1033788/1‑150 (MQ=255)
                       ccAGCTACGACGACGCCCTGCTGCGCCGTGAAGTGGAACTGTTCCCCGAATGGTACGTGGGGCGTGAGCTGGGCCTGACCTTCACCGATGCACAGAAGGCCACCTGGCAGCGCGTCAGCCAATTGCTGATCGACAGCGCCCTGGCGCAGc                                                                                                                           >  2:1033957/1‑150 (MQ=255)
                       ccAGCTACGACGACGCCCTGCTGCGACGGGAAGTGGAACTGTTCCCCGAATGGTACGTGGGGCGTGAGCTGGGCCTGACCTTCACCGATGCACAGAAGGCCACCTGGCAGCGCGTCAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGc                                                                                                                           >  1:263117/1‑150 (MQ=255)
                        cAGCTACGACGACGCCCTGCTGCGCCGTGAAGTGGAACTGTTCCCCGAATGGTACGTGGGGCGTGAGCTGGGCCTGACCTTCACCGATGCACAGAAGGCCACCTGGCAGCGCGTCAGCCAGTTGCTGATCGACAGCGCCCTGgc                                                                                                                                >  1:128644/1‑144 (MQ=255)
                        cAGCTACGACGACGCCCTGCTGCGCCGTGAAGTGGAACTGTTCCCCGAATGGTACGTGGGGCGTGAGCTGGGCCTGACCTTCACCGATGCACAGAAGGCCACCTGGCAGCGCGTCAGCCAGTTGCTGATCGACAGCGCCCTGgc                                                                                                                                <  2:128644/144‑1 (MQ=255)
                            tACGACGACGCCCTGCTGCGCCGTGAAGTGGAACTGTTCCCCGAATGGTACGTGGGGCGTGAGCTGGGCCTGACCTTCACCGATGCACAGAAGGCCACCTGGCAGCGCGTCAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAg                                                                                                                      >  1:543081/1‑150 (MQ=255)
                                acgacgCCCTGCTGCGCCGTGAAGTGGAACTGTTCCCCGAATGGTACGTGGGGCGTGAGCTGGGCCTGACCTTCACCGATGCACAGAAGGCCACCTGGCAGCACGTCAGTCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGc                                                                                                                  <  1:83622/150‑1 (MQ=255)
                                  gacgCCCTGCTGCGCCGTGAAGTGGAACTGTTCCCCGAATGGTACGTGGGGCGTGAGCTGGGCCTGACCTTCACCGATGCACAGAAGGCCACCTGGCAGCGCGTCAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTg                                                                                                                <  2:1026509/150‑1 (MQ=255)
                                     gCCCTGCTGCGCCGTGAAGTGGAACTGTTCCCCGAATGGTACGTGGGGCGTGAGCTGGGCCTGACCTTCACCGATGCACAGAAGGCCACCTGGCAGCGCGTCAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTa                                                                                                             >  1:755496/1‑150 (MQ=255)
                                       ccTGCTGCGCCGTGAAGTGGAACTGTTCCCCGAATGGTACGTGGGGCGTGAGCTGGGCCTGACCTTCACCGATGCACAGAAGGCCACCTGGCAGCGCGTCAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTaca                                                                                                           >  2:211448/1‑150 (MQ=255)
                                                        tGGAACTGTTCCCCGAATGGTACGTGGGGCGTGAGCTGGGCCTGACCTTCACCGATGCACAGAAGGCCACCTGGCAGCGCGTCAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGCcgc                                                                                          <  2:543081/150‑1 (MQ=255)
                                                               gTTCCCCGAATGGTACGTGGGGCGTGAGCTGGGCCTGACCTTCACCGATGCACAGAAGGCCACCTGGCAGCGCGTCAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGCCGCGCAACCt                                                                                   <  2:755496/150‑1 (MQ=255)
                                                                  ccccGAATGGTACGTGGGGCGTGAGCTGGGCCTGACCTTCACCGATGCACAGAAGGCCACCTGGCAGCGCGTCAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGCCGCGCAACCTGAt                                                                                >  2:1006826/1‑150 (MQ=255)
                                                                   cccGAATGGTACGTGGGGCGTGAGCTGGGCCTGACCTTCACCGATGCACAGAAGGCCACCTGGCAGCGCGTCAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGCCGCGCAACCTGATg                                                                               >  1:995383/1‑150 (MQ=255)
                                                                   cccGAATGGTACGTGGGGCGTGAGCTGGGCCTGACCTTCACCGATGCACAGAAGGCCACCTGGCAGCGCGTCAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGCCGCGCAACCTGATg                                                                               >  2:845627/1‑150 (MQ=255)
                                                                                 ggggCGTGAGCTGGGCCTGACCTTCACCGATGCACAGAAGGCCACCTGGCAGCGCGTCAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCaa                                                                 <  2:877579/150‑1 (MQ=255)
                                                                                    gCGTGAGCTGGGCCTGACCTTCACCGCTGCACAGAAGGCCACCTGGCAGCGCGTCAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAAccc                                                              >  1:315788/1‑150 (MQ=255)
                                                                                      gTGAGCTGGGCCTGACCTTCACCGATGCACAGAAGGCCACCTGGCAGCGCGTCAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTg                                                            <  2:995383/150‑1 (MQ=255)
                                                                                         aGCTGGGCCTGACCTTCACCGATGCACAGAAGGCCACCTGGCAGCGCGTCAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTTGTACACCGTGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGgtg                                                         <  2:429664/150‑1 (MQ=255)
                                                                                            tGGGCCTGACCTTCACCGATGCACAGAAGGCCACCTGGCAGCGCGTCAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGc                                                      >  2:394652/1‑150 (MQ=255)
                                                                                              ggCCTGACCTTCACCGATGCACAGAAGGCCACCTGGCAGCGCGTCAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTg                                                    <  1:534355/150‑1 (MQ=255)
                                                                                              ggCCTGACCTTCACCGATGCACAGAAGGCCACCTGGCAGCGCGTCAGCCAATTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTg                                                    <  1:1033957/150‑1 (MQ=255)
                                                                                              ggCCTGACCTTCACCGATGCACAGAAGGCCACCTGGCAGCGCGTCAGCCAATTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTg                                                    <  1:1033788/150‑1 (MQ=255)
                                                                                                ccTGACCTTCACCGATGCACAGAAGGCCACCTGGCAGCGCGTCAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGcc                                                                                            <  2:99059/108‑1 (MQ=255)
                                                                                                ccTGACCTTCACCGATGCACAGAAGGCCACCTGGCAGCGCGTCAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGcc                                                                                            >  1:99059/1‑108 (MQ=255)
                                                                                                ccTGACCTTCACCGATGCACAGAAGGCCACCTGGCAGCGCGTCAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGCCGCGCAACCTGATGCaga                                                                           >  1:141642/1‑125 (MQ=255)
                                                                                                ccTGACCTTCACCGATGCACAGAAGGCCACCTGGCAGCGCGTCAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGCCGCGCAACCTGATGCaga                                                                           <  2:141642/125‑1 (MQ=255)
                                                                                                     ccTTCACCGATGCACAGAAGGCCACCTGGCAGCGCGTCAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTc                                             <  1:622708/150‑1 (MQ=255)
                                                                                                       ttCACCGATGCACAGAAGGCCACCTGGCAGCGCGTCAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAg                                           <  1:635902/150‑1 (MQ=255)
                                                                                                           ccGATGCACAGAAGGCCACCTGGCAGCGCGTCAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGCCGCGCAACCTGATGGTTAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATg                                       >  1:608222/1‑150 (MQ=255)
                                                                                                              aTGCACAGAAGGCCACCTGGCAGCGCGTCAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCgg                                    <  1:871896/150‑1 (MQ=255)
                                                                                                                    aGAAGGCCACCTGGCAGCGCGTCAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTacac                                                                                                          <  1:552815/74‑1 (MQ=255)
                                                                                                                    aGAAGGCCACCTGGCAGCGCGTCAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTacac                                                                                                          >  2:552815/1‑74 (MQ=255)
                                                                                                                        ggCCACCTGGCAGCGCGTCAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGccc                          >  1:926701/1‑150 (MQ=255)
                                                                                                                              cTGGCAGCGCGTCAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCAc                    >  1:693482/1‑150 (MQ=255)
                                                                                                                                   aGCGCGTCAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACg               >  1:1037714/1‑150 (MQ=255)
                                                                                                                                    gcgcGTCAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGa              <  2:194121/150‑1 (MQ=255)
                                                                                                                                     cgcgTCAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGAc             <  2:315788/150‑1 (MQ=255)
                                                                                                                                      gcgTCAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGACa            <  2:926701/150‑1 (MQ=255)
                                                                                                                                        gTCAGCCAGTTGCTGATCGACAGCGCCCNGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGACAtt          >  1:704181/1‑150 (MQ=255)
                                                                                                                                          cAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGACATTAc        <  1:211448/150‑1 (MQ=255)
                                                                                                                                                gTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGACATTAcctgcct  >  1:1064756/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
TTTGCCAATGGACGCGCCGCTGCCCAGCTACGACGACGCCCTGCTGCGCCGTGAAGTGGAACTGTTCCCCGAATGGTACGTGGGGCGTGAGCTGGGCCTGACCTTCACCGATGCACAGAAGGCCACCTGGCAGCGCGTCAGCCAGTTGCTGATCGACAGCGCCCTGGCGCAGCCCAAGGTGCTGGTACACCGTGACTACATGCCGCGCAACCTGATGCAGAGCACCCCCAACCCTGGTGTGCTGGACTTTCAGGATGCGGTATACGGCCCGGTCACCTACGACATTACCTGCCT  >  NC_002947/492958‑493251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑