Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,834,371 A→G 10.8% N198S (AAC→AGC)  PP_5113 → Zn‑dependent peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,834,3710AG10.8% 90.3 / 3.1 38N198S (AAC→AGC) PP_5113Zn‑dependent peptidase
Reads supporting (aligned to +/- strand):  ref base A (18/15);  new base G (2/2);  total (20/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.77e-01

CGCATCGCTGCGCAGCCTCAGTGCCAAGGACAAGCGCGAACCGGCCCTCAAACTGTTTACAGAGGTGGCGGGCAAGCCGACATTCCCCGAGGATGCCCTCAAGCGCATCAAGAACCAGATGCTGGCTGGCTTCGAGTATGAGAAGCAAAACCCCGGCAAGATCGCCGGCAAGGCCCTTTTCGGCAAGCTCTATGGCGACCACCCCTACGCCCACCCAAGCGACGGCACCGCCGAGAGCATCACGGGCATCAGCCTTGCGCAGTTGCGCGCGTTCCACGCAAAGGCCTATACCGGCGGC  >  NC_002947/5834222‑5834519
                                                                                                                                                     |                                                                                                                                                    
cGCATCGCTGCGCAGCCTCAGTGCCAAGGACAAGCGCGAACCGGCCCTCAAACTGTTTACAGAGGTGGCGGGCAAGCCGACATTCCCCGAGGATGCCCTCAAGCGCATCAAGAACCAGATGCTGGCTGGCTTCGAGTATGAGAAGCaaaa                                                                                                                                                      <  1:573301/150‑1 (MQ=255)
   aTCGCTGCGCAGCCTCAGTGCCAAGGACAAGCGCGAACCGGCCCTCAAACTGTTTACAGAGGTGGCGGGCAAGCCGACATTCCCCGAGGATGCCCTCAAGCGCATCAAGAACCAGATGCTGGCTGGCTTCGAGTATGAGAAGCAAAAccc                                                                                                                                                   >  2:1057057/1‑150 (MQ=255)
     cGCTGCGCAGCGTCAGTGACAAGGACAAGCGGGAACCGGCCCTCAAACTGTTTACAGAGGTGGCGGGCAAGCCGACATTCCCCGAGGATGCCCTCAAGCGCATCAAGAACCAGATGCTGGCTGGCTTCGAGTATGAGAAGCAAAACCCCg                                                                                                                                                 <  2:55846/150‑1 (MQ=255)
         gcgcAGCCTCAGTGCCAAGGCCGAGCGCGCCGCGGCCCGCGAACTGTTTACAGAGGTGGAAGAAATCGCACTGCTGCCCGAGGATGCCCTCAAGCGCATCAAGAACCAGATGCTGGCTGGCTTCGAGTATGAGAAGCAAAACCCCGGCaa                                                                                                                                             <  1:748706/150‑1 (MQ=255)
              gCCTCAGTGCCAAGGACAAGCGCGAACCGGCCCTCAAACTGTTTACAGAGGTGGCGGGCAAGCCGACATTCCCCGAGGATGCCCTCAAGCGCATCAAGAACCAGATGCTGGCTGGCTTCGAGTATGAGAAGCAAAACCCCGGCAAGATCg                                                                                                                                        >  1:614622/1‑150 (MQ=255)
               ccTCAGTGCCAAGGACAAGCGCGAACCGGCCATCAAACTGTTTACAGAGGTGGCGGGCAAGCCGACATTCCCCGAGGATGCCCTCAAGCGCATCAAGAACCAGATGCTGGCTGGCTTCGAGTATGAGAAGCAAAACCCCGGCAAGATCGc                                                                                                                                       >  1:635371/1‑150 (MQ=255)
                    gTGCCAAGGACAAGCGCGAACCGGCCCTCAAACTGTTTACAGAGGTGGCGGGCAAGCCGACATTCCCCGAGGATGCCCTCAAGCGCATCAAGAACCAGATGCTGGCTGGCTTCGAGTATGAGAAGCAAAACCCCGGCAAGATCGCCGGCa                                                                                                                                  <  2:18180/150‑1 (MQ=255)
                     tGCAAAGGACAGACGCGAACCGGCCCTCAAGCTGTTTACAGAGGTGGCGGGCAAGCCGACATTCCCCGAGGATGCCCTCAAGCGCATCAAGAACCAGATTCTGGCTGGCTTCGAGTATGAGAAGCAAAACCCCGGCAAGATCGCCGGCaa                                                                                                                                 <  2:614622/150‑1 (MQ=255)
                       cgaaGGACAAGCCCAAACCGGCCCTAAAACTGTTTACAGAGGTCGCCGTCAAGCCGATATTCCCCGAGGATGCCCTCAACCGCAGCAACCAACATAAAATAGGTAGCGACTAGTATGACAATCAAAACCCCGGCAAGATCGCCGGCAAgg                                                                                                                               <  1:467488/148‑1 (MQ=255)
                               aaGCGCGAACCGGCCCTCAAACTGTTTACAGAGGGGGCGGGCAAGCCGACATTCCCCGAGGATGCCCTCAAGCGCATCAAGAACCAGATGCTGGCTGGCTTCGAGTATGAGAAGCAAAACCCCGGCAAGATCGCCGGCAAGGCCCTTTTc                                                                                                                       <  2:330792/150‑1 (MQ=255)
                                        ccGGCCCTCAAACTGTTTACAGAGGTGGCGGGCAAGCCGACATTCCCCGAGGATGCCCTCAAGCGCATCAAGAACCAGATGCTGGCTGGCTTCGAGTATGAGAAGCAAAACCCCGGCAAGATCGCCGGCAAGGCCCTTTTCGGCAAGctc                                                                                                              >  1:473388/1‑150 (MQ=255)
                                               tCAAACTGTTTACAGAGGTGGCGGGCAAGCCGACATTCCCCGAGGATGCCCTCAAGCGCATCAAGAACCAGATGCTGGCTGGCTTCGAGTATGAGAAGCAAAACCCCGGCAAGATCGCCGGCAAGGCCCTTTTCGGCAAGCTCTATGGCg                                                                                                       >  1:540889/1‑150 (MQ=255)
                                                             gagGTGGCGGGCAAGCCGACATTCCCCGAGGATGCCCTCAAGCGCATCAAGAACCAGATGCTGGCTGGCTTCGAGTATGAGAAGCAAACCCCCGGCAAGATCGCCGGCAAGGCCCTTTTCGGCAAGCTCTATGGCGACCACCCCTACGcc                                                                                         <  2:941850/150‑1 (MQ=255)
                                                              agGTGGCGGGCAAGCCGACATTCCCGGAGGATGAGCTCAAGCGCATCAAGAACCAGATGCTGGCTGGCTTCGAGTATGAGAAGCAAAACCCCGGCAAGATCGCCGGCAAGGCCCTTTTCGGCAAGCGCTATGGCGACCACCCCTACGccc                                                                                        >  1:533197/1‑150 (MQ=255)
                                                                 tGGCGGGCAAGCCGACATTCCCCGAGGATGCCCTCAAGCGCATCAAGAACCAGATGCTGGCTGGCTTCGAGTATGAGAAGCAAAACCCCGGCAAGATCGCCGGCAAGGCCCTTTTCGGCAAGCTCTATGGCGACCACCCCTACGcccacc                                                                                     >  2:349556/1‑150 (MQ=255)
                                                                         aaGCCGACATTCCCCGAGGATGCCCTCAAGCGCATCAAGAACCAGATGCTGGCTGGCTTCGAGTATGAGAAGCAAAACCCCGGCAAGATCGCCGGCAAGGCCCTTTTCGGCAAGCTCTATGGCGACCACCCCTACGCCCACCCAAGCGAc                                                                             <  2:76800/150‑1 (MQ=255)
                                                                                     cccGAGGATGCCCTCAAGCGCATCAAGAACCAGATGCTGGCTGGCTTCGAGTATGAGAAGCAAAACCCCGGCAAGATCGCCGGCAAgg                                                                                                                               >  1:36083/1‑88 (MQ=255)
                                                                                     cccGAGGATGCCCTCAAGCGCATCAAGAACCAGATGCTGGCTGGCTTCGAGTATGAGAAGCAAAACCCCGGCAAGATCGCCGGCAAgg                                                                                                                               <  2:36083/88‑1 (MQ=255)
                                                                                      ccGAGGATGCCCTCAAGCGCATCAAGAACCAGATGCTGGCTGGCATCGAGGATGAGAAGCAAAACCCCGGCAAGATCGCCGGCAAGGCCCTTTTCGGCAAGCTCTATGGCGACCACCCCTACGCCCACCCAAGCGACGGCACCGCCgaga                                                                >  2:231919/1‑150 (MQ=255)
                                                                                      ccGAGGATGCCCTCAAGCGCATCAAGAACCAGATGCCTGTTGGCTTCGAGTATGAGAAGCAAAACCCCGGCAAGATCGCCGGCAAGGCCCTTTTCGGCAAGCTCTATGGCGACCACCCCTACGCCCACCCAAGCGACGGCACCGCCgaga                                                                <  2:533197/150‑1 (MQ=255)
                                                                                                  tCAAGCGCATCAAGAACCAGATGCTGGCTGGCTTCGAGTATGAGAAGCAAAACCCCGGCAAGATCGCCGGCAAGGCCCTTTTCGGCAAGCTCTATGGCGac                                                                                                     <  2:665609/101‑1 (MQ=255)
                                                                                                  tCAAGCGCATCAAGAACCAGATGCTGGCTGGCTTCGAGTATGAGAAGCAAAACCCCGGCAAGATCGCCGGCAAGGCCCTTTTCGGCAAGCTCTATGGCGac                                                                                                     >  1:665609/1‑101 (MQ=255)
                                                                                                          accAAGAACCAGATGCTGGCTGGCTTCGAGTATTAGAAGCAAAACCCCTGCAAGATCGCCGGCAAGGCCCTTTTCGGCAAGCTTTATGGCGACCCCCCCTACGCCCACCCAAGCGAAGGCACCGCCGAGAGCATCACGGGCATCAGCCtt                                            <  1:383561/148‑1 (MQ=255)
                                                                                                           tCAAGAACCAGATGCTGGCTGGCTTCGAGTATGAGAAGCAAAACCCCGGCAAGATCGCCGGCAAGGCCCTTTTCGGCAAGCTCTATGGCGACCACCCCTACGCCCACCCAAGCGACGGCACCGCCGAGAGCATCACGGGCATCAGCCTTg                                           >  1:635564/1‑150 (MQ=255)
                                                                                                                    aGATGCTGGCTGGCTTCGAGTATGAGAAGCAAAACCCCGGCAAGATCGCCGGCAAGGCCCTTTTCGGCAAGCTCTATGGCGACCACCCCTACGCCCACCCAAGCGACGGCACCGCCGAGAGCATCACGGGCATCAGCCTTGCGCAGtttc                                  >  1:165569/1‑148 (MQ=255)
                                                                                                                    aGATGCTGGCTGGCTTCGAGTATGAGAAGCAAAACCCCGGCAAGATCGCCGGCAAGGCCCTTTTCGGCAAGCTCTATGGCGACCACCCCTACGCCCACCCAAGCGACGGCACCGCCGAGAGCATCACGGGCATCAGCCTTGCGCAGTTgc                                  <  2:473388/150‑1 (MQ=255)
                                                                                                                    aGATGCTGGCTGGCTTCGAGTATGAGAAGCAAAACCCCGGCAAGATCGCCGGCAAGGCCCTTTTCGGCAAGCTCTATGGCGACCACCCCTACGCCCACCCAAGCGACGGCACCGCCGAGAGCATCACGGGCATCAGCCTTGCGCAGTTgc                                  >  1:458524/1‑150 (MQ=255)
                                                                                                                     gATGCTGGCTGGCTTCGAGTATGAGAAGCAAAACCCCGGCAAGATCGCCGGCAAGGCCCTTTTCGGCAAGCTCTATGGCGACCACCCCTACGCCCACCCAAGCGACGGCACCGCCGAGAGCATCACGGGCATCAGCCTTGCGCAGTTgcg                                 >  2:198026/1‑150 (MQ=255)
                                                                                                                          tggctggcTTCGAGTATGAGAAGCAAAACCCCGGCAAGATCGCCGGCAAGGCCCTTTTCGGCAAGCTCTATGGCGACGACCCCTACGCCCACCCAAGCGACGGCACCGCCGAGAGCATCACGGCCATCAGCCTTGCGCAGTTGCGCGCGt                            >  2:288361/1‑150 (MQ=255)
                                                                                                                              tggctTCGAGTATGAGAAGCAAAACCCCGGCAAGATCGCCGGCAAGGCCCTTTTCGGCAAGCTCTATGGCGACCAcccc                                                                                               <  2:481603/79‑1 (MQ=255)
                                                                                                                              tggctTCGAGTATGAGAAGCAAAACCCCGGCAAGATCGCCGGCAAGGCCCTTTTCGGCAAGCTCTATGGCGACCAcccc                                                                                               >  1:481603/1‑79 (MQ=255)
                                                                                                                              tggctTCGAGTATGAGAAGCAAAACCCCGGCAAGATCGCCGGCAAGGCCCTTTTCGGCAAGCTCTATGGCGACCACCCCTACGCCCACCCAAGCGACGGCACCGCCGAGAGCATCACGGGCATCAGCCTTGCGCAGTTGCGCGCGTTCCa                        >  1:430013/1‑150 (MQ=255)
                                                                                                                               ggctTCGAGTATGAGAAGCAAAACCCCGGCAAGATCGCCGGCAAGGCCCTTTTCGGCAAGCTCTATGGCGACCACCCCTACGCCCACCCAAGCGACGGCACCGCCGAGAGCATCACGGGCATCAGCCTTGCGCAGTTGCGCGCGTTCCAc                       <  1:198026/150‑1 (MQ=255)
                                                                                                                                          tGAGAAGCAAAACCCCGGCAAGATCGCCGGCAAGGCCCTTTTCGGCAAGCTCTATGGCGACCACCCCTACGCCCACCCAAGCGACGGCAc                                                                        <  2:546718/90‑1 (MQ=255)
                                                                                                                                          tGAGAAGCAAAACCCCGGCAAGATCGCCGGCAAGGCCCTTTTCGGCAAGCTCTATGGCGACCACCCCTACGCCCACCCAAGCGACGGCAc                                                                        >  1:546718/1‑90 (MQ=255)
                                                                                                                                             gaAGCAAAGCCCCGGCAAGATCGCCGGCAAGGCCCTTTTCGGCAAGCTCTATGGCGACCACCCCTACGCCCACCCAAGCGACGGCACCGCCGAGAGCATCACGGGCAt                                                   <  2:294146/108‑1 (MQ=255)
                                                                                                                                             gaAGCAAAGCCCCGGCAAGATCGCCGGCAAGGCCCTTTTCGGCAAGCTCTATGGCGACCACCCCTACGCCCACCCAAGCGACGGCACCGCCGAGAGCATCACGGGCAt                                                   <  2:294961/108‑1 (MQ=255)
                                                                                                                                             gaAGCAAAGCCCCGGCAAGATCGCCGGCAAGGCCCTTTTCGGCAAGCTCTATGGCGACCACCCCTACGCCCACCCAAGCGACGGCACCGCCGAGAGCATCACGGGCAt                                                   >  1:294961/1‑108 (MQ=255)
                                                                                                                                             gaAGCAAAGCCCCGGCAAGATCGCCGGCAAGGCCCTTTTCGGCAAGCTCTATGGCGACCACCCCTACGCCCACCCAAGCGACGGCACCGCCGAGAGCATCACGGGCAt                                                   >  1:294146/1‑108 (MQ=255)
                                                                                                                                                    aaCCCCGGCAAGATCGCCGGCAAGGGCCTTTTCGGCAAGCTCTATGGCGACCACCCCTACGCCCACCCAAGCGCCGGCACCGCCGAGAGCATCACGGGCATCAGCCTTCCGCAGATGCGCACGTTCCACGCCAAGACCTATCCCggcggc  >  2:77097/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGCATCGCTGCGCAGCCTCAGTGCCAAGGACAAGCGCGAACCGGCCCTCAAACTGTTTACAGAGGTGGCGGGCAAGCCGACATTCCCCGAGGATGCCCTCAAGCGCATCAAGAACCAGATGCTGGCTGGCTTCGAGTATGAGAAGCAAAACCCCGGCAAGATCGCCGGCAAGGCCCTTTTCGGCAAGCTCTATGGCGACCACCCCTACGCCCACCCAAGCGACGGCACCGCCGAGAGCATCACGGGCATCAGCCTTGCGCAGTTGCGCGCGTTCCACGCAAAGGCCTATACCGGCGGC  >  NC_002947/5834222‑5834519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑