Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,903,221 A→C 13.2% intergenic (‑129/‑139) PP_5175 ← / → PP_5176 HlyD family secretion protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,903,2210AC13.2% 97.3 / 10.3 48intergenic (‑129/‑139)PP_5175/PP_5176HlyD family secretion protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (22/18);  new base C (3/3);  total (27/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.91e-01

CAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCATCCCCGTTCCAACCGCTACCTTTCACAGGGAGGCTCACATTC‑AAGGAAGATCAAAATGCCGTTAGCCGAATGCCACCGA  >  NC_002947/5903072‑5903369
                                                                                                                                                     |                                                                                                                                                     
cAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGa                                                                                                                                                       <  1:711883/150‑1 (MQ=255)
         cATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATAATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGaa                                                                                                                                              <  1:695989/150‑1 (MQ=255)
          aTCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAag                                                                                                                                             >  1:431696/1‑150 (MQ=255)
           tCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAaga                                                                                                                                            >  2:735857/1‑150 (MQ=255)
           tCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAaga                                                                                                                                            <  1:475318/150‑1 (MQ=255)
             aaCGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCTCGCCCGCGAaga                                                                                                                                            <  1:227762/148‑1 (MQ=255)
             aaCGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCTCGCCCGCGAaga                                                                                                                                            >  2:227762/1‑148 (MQ=255)
                 ccATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTc                                                                                                                                      >  1:557020/1‑150 (MQ=255)
                   aTGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCTCGCCCGCGAaga                                                                                                                                            >  1:199959/1‑142 (MQ=255)
                   aTGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCTCGCCCGCGAaga                                                                                                                                            <  2:199959/142‑1 (MQ=255)
                                   tgtgCACAAATGATTTGAGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCTGCGAAGAGGCCTCCACAGCCATGTATCAGTa                                                                                                                    >  2:346926/1‑150 (MQ=255)
                                       cacaAATGATTTGTGGGATGCGACTGTGAGCGGAGTTGAGGGTTCGCTAAGAATCACTGACGGGGGCCATGCTGGCAGTTTTGGTGCCTGCGGGGGGCTCTGCTCGGGGGGGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTg                                                                                                                >  1:631912/1‑150 (MQ=255)
                                        acaAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGc                                                                                                               >  1:442389/1‑150 (MQ=255)
                                         caAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCAACAGCCATGTATCAGTATCTGcc                                                                                                              <  2:557020/150‑1 (MQ=255)
                                             tGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCatat                                                                                                          <  2:470551/150‑1 (MQ=255)
                                                  tgtgGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACCTCTGTTTCGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGTCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGa                                                                                                     <  1:237969/150‑1 (MQ=255)
                                                   gtgGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGaa                                                                                                    >  1:586661/1‑150 (MQ=255)
                                                          gccaCTGTGAGCGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCa                                                                                             <  2:437644/147‑1 (MQ=255)
                                                              cTGTGAGCGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCGCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCa                                                                                         >  1:1091971/1‑150 (MQ=255)
                                                               tgtgAGCGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCAt                                                                                        >  1:685735/1‑150 (MQ=255)
                                                                  gAGCGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCgg                                                                                     <  1:358046/150‑1 (MQ=255)
                                                                   aGCGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGc                                                                                    >  2:274807/1‑150 (MQ=255)
                                                                         gTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCATGCATCGGCCATccc                                                                              >  1:500730/1‑150 (MQ=255)
                                                                          ttCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCGATATCCAGAAAACATCAGGCATCGGCCATcccc                                                                             >  1:731219/1‑150 (MQ=255)
                                                                                     aTANACATCACTGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCATCCCCGTTCCAACCGc                                                                  >  2:697449/1‑150 (MQ=255)
                                                                                      tAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAaga                                                                                                                                            >  1:1088412/1‑75 (MQ=255)
                                                                                      tAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAaga                                                                                                                                            <  2:1088412/75‑1 (MQ=255)
                                                                                               cTGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCATCCCCGTTCCAACCGCTACCTTTcac                                                        >  2:986415/1‑150 (MQ=255)
                                                                                                  aCGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTAt                                                                                                                         >  2:392572/1‑82 (MQ=255)
                                                                                                  aCGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTAt                                                                                                                         <  1:392572/82‑1 (MQ=255)
                                                                                                      gggCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCATCCCCGtt                                                                          >  1:549507/1‑125 (MQ=255)
                                                                                                      gggCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCATCCCCGtt                                                                          <  2:549507/125‑1 (MQ=255)
                                                                                                            tGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCATCCCCGTTCCAACCGCTACCTTTCACAGGGAGGCTcaca                                           <  2:500730/150‑1 (MQ=255)
                                                                                                               tGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCATCCCCGTTCCAACCGCTACCTTTCACAGGGAGGCTCACATTc                                        >  1:198860/1‑150 (MQ=255)
                                                                                                                  cATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCATCCCCGTTCCAACCGCTACCTTTCACAGGGAGGCTCACATTC‑AAg                                    <  2:586661/150‑1 (MQ=255)
                                                                                                                     ttttGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCATCCCCGTTCCAACCGCTACCTTTCACAGGGAgg                                                 <  2:152600/135‑1 (MQ=255)
                                                                                                                     ttttGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCATCCCCGTTCCAACCGCTACCTTTCACAGGGAgg                                                 >  1:152600/1‑135 (MQ=255)
                                                                                                                     ttttGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCATCCCCGTTCCAACCGCTACCTTTCACAGGGAGGCTCACATTC‑AAGGaa                                 <  1:274807/150‑1 (MQ=255)
                                                                                                                                    gggCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGaaa                                                                                                   >  2:641912/1‑70 (MQ=255)
                                                                                                                                    gggCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGaaa                                                                                                   <  1:641912/70‑1 (MQ=255)
                                                                                                                                       ccTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCATCCCCGTTCCAACCGCTACCTTTCACAGGGAGGCTCACATTC‑AAGGAAGATCAAAATGCCGTTAGc               <  1:986415/150‑1 (MQ=255)
                                                                                                                                       ccTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCATCCCCGTTCCAACCGCTACCTTTCACAGGGAGGCTCACATTC‑AAGGAAGATCAAAATGCCGTTAGc               <  1:735857/150‑1 (MQ=255)
                                                                                                                                        ctctTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCATCCCCGTTCCAACCGCTACCTTTCACAGGGAGGCTCACATTC‑AAGGaa                                 >  1:169981/1‑131 (MQ=255)
                                                                                                                                        ctctTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCATCCCCGTTCCAACCGCTACCTTTCACAGGGAGGCTCACATTC‑AAGGaa                                 <  2:169981/131‑1 (MQ=255)
                                                                                                                                           ttCGCGGGCTCGCCCGCGAAGAGGCCCCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCAt                                                                                               <  2:1127662/67‑1 (MQ=25)
                                                                                                                                           ttCGCGGGCTCGCCCGCGAAGAGGCCCCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCAt                                                                                               >  1:1127662/1‑67 (MQ=25)
                                                                                                                                           ttCGCGGGCGGGCCCGCGGAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATAACCAGAAACCATCAGGCATCGGCCATCCCCGTTCCAACCCCTACCTTTAACAAGCAGGCT‑ACGGCCGAAGGAACAGCAAAATTCCGTGAGCCGaa           >  2:966323/1‑150 (MQ=255)
                                                                                                                                                    gAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCATCCCCGTTCCAACCGCTACCTTTCACAGGGAGGCTCACATTC‑AAGGAAGATCAAAATGCCGTTAGCCGAATGCCACCGa  >  1:54829/1‑150 (MQ=255)
                                                                                                                                                    gAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCATCCCCGTTCCAACCGCTACCTTTCACAGGGAGGATCACATTC‑AAGGAAGATCAAAATGCCGTTAGCCGAATGCCACCGa  >  1:212627/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCACTGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCATCCCCGTTCCAACCGCTACCTTTCACAGGGAGGCTCACATTC‑AAGGAAGATCAAAATGCCGTTAGCCGAATGCCACCGA  >  NC_002947/5903072‑5903369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑