| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,052,931 | G→T | 8.6% | L198M (CTG→ATG) ‡ | PP_5305 ← | epimerase/dehydratase‑like protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,052,931 | 0 | G | T | 8.6% | 174.5 / 4.6 | 58 | L198M (CTG→ATG) ‡ | PP_5305 | epimerase/dehydratase‑like protein |
| Reads supporting (aligned to +/- strand): ref base G (28/25); new base T (2/3); total (30/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01 | |||||||||||
TCATCGGACCATTCGGTAACCCCCATGTAGGCCCGCAGCCAGGCCACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGACGCACCTGGCTCAGCAACCACTCGCGCCCTGGGCCATAGATACCGGTCAGGCGGACCACGCTCGCCGGGATGCCGCTG > NC_002947/6052789‑6053079 | tCATCGGACCATTCGGTAACCCCCATGTAGGCCCGCAGCCAGGCCACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGATCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCCTGCTggcg > 1:249152/1‑150 (MQ=255)tCATCGGACCATTCGGTAACCCCCATGTAGGCCCGCAGCCAGGCCACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCCTCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTggcg < 2:48185/150‑1 (MQ=255) cATCGGACCATTCGGTAACCCCCATGTAGGCCCGCAGCCAGGCCACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCCTGCTggcgt < 1:174584/150‑2 (MQ=255) tCGGACCATTCGGTAACCCCCATGTAGGCCCGCAGCCAGGCCACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCCTGCTggcgttt < 2:249152/150‑4 (MQ=255) cGGACCATTCGGTAACCCCCATGTAGGCCCGCAGCCAGGCCACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCCTGCTggcgtttc < 1:811637/150‑5 (MQ=255) aCCATTCGGTAACCCCCATGTAGGCCCGCAGCCAGGCCACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCtt > 2:881896/1‑150 (MQ=255) aaCCCCCATGTAGGCCCGCAGCCAGGCCACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAAt < 1:461412/150‑1 (MQ=255) aaCCCCCATGTAGGCCCGCAGCCAGGCCACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAAt < 1:461764/150‑1 (MQ=255) ccccATGTAGGCCCGCAGCCAGGCCACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCGGCAGAAACGCCAGCAGGCGGGCGGCATCTTCGGCATGAATGCg > 2:969134/1‑150 (MQ=255) ccccATGTAGGCCCGCAGCCAGGCCACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCg > 2:877988/1‑150 (MQ=255) ccccATGTAGGCCCGCAGCCAGGCCACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCg < 2:644549/150‑1 (MQ=255) ccccATGTAGGCCCGCAGCCAGGCCACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCg < 2:903136/150‑1 (MQ=255) ccccATGTAGGCCCGCAGCCAGGCCACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCg > 2:599051/1‑150 (MQ=255) tGTAGGCCCGCAGCCAGGCCACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGc > 2:1047581/1‑150 (MQ=255) tAGGCCCGCAGCCAGGCCACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTGGCAGTCGTCCAGAGCCTTGCCATCAACATCGGCCTACCGCAGAAACGCCATCAGGCTCACGGCATCTTAGGCAAGAATGCGATTGCCa > 2:68231/1‑150 (MQ=255) cccGCAGCCAGGCCACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACa > 1:951802/1‑150 (MQ=255) aGCCAGGCCACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCCCGTGGATGAATGCGGTTGCCATACAgcggc < 1:1051645/150‑1 (MQ=255) gcaaGGCCACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGACAATGTATCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAgcggcg > 1:476159/4‑150 (MQ=255) gCCAGGCCACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAgcggcg > 2:111531/1‑150 (MQ=255) ccAGGCCACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGcggcgg > 2:981320/1‑150 (MQ=255) gCCACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGctcc > 2:399469/1‑150 (MQ=255) ccaccaCATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGctcct < 1:881896/150‑1 (MQ=255) ccacATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCgg > 2:349030/1‑150 (MQ=255) aTCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAAc > 1:184718/1‑150 (MQ=255) tCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTg < 1:672537/96‑1 (MQ=255) tCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTg > 2:672537/1‑96 (MQ=255) ggCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAAcgcg < 2:1063769/150‑1 (MQ=255) gCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCgg < 1:1047581/150‑1 (MQ=255) gCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCgg < 1:399469/150‑1 (MQ=255) ccAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGt < 1:199726/150‑1 (MQ=255) gcggcgCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAAc < 2:708923/150‑1 (MQ=255) gcCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGa < 2:184718/150‑1 (MQ=255) gtcgtcATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAgcggc < 1:552137/118‑1 (MQ=255) gtcgtcATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAgcggc > 2:552137/1‑118 (MQ=255) gtcATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATg > 2:407718/1‑97 (MQ=255) gtcATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATg < 1:407718/97‑1 (MQ=255) tcatcaACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGACGCACCTgg > 2:873672/1‑150 (MQ=255) tcatcaACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGACGCACCTgg > 1:166858/1‑150 (MQ=255) atcaACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGACGCACCTGGCt > 2:949705/1‑150 (MQ=255) atcaACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGCCATATTCGGCATGAATGCGGTTGCCATACAGCGGCGGCGCGTCGGCAACGCGGGGGCCCTGACACACCTGGCt > 1:299122/1‑150 (MQ=255) caACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGACGCACCTGGCTCa < 1:111531/150‑1 (MQ=255) aaCGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCGCCTCGGCAACGCGGTAACCCTGACGCACCTGGCTCAg > 1:173829/1‑150 (MQ=255) gCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGACGCACCTGGCTCAGCaa < 1:981320/150‑1 (MQ=255) tAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGACGCACCTGGCTCAGCAACCACTcg > 2:430488/1‑150 (MQ=255) aGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGACGCACCTGGCTCAGCAACCACTcgc < 1:877988/150‑1 (MQ=255) gCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGACGCACCTGGCTCAGCAACCACTcgcg > 2:825254/1‑150 (MQ=255) agagCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGATGCACCTGGCTCAGCAACCACTCGCGCCCTGGGCCa > 2:711080/1‑150 (MQ=255) agCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGACGCACCTGGCTCAGCAACCACTCGCGCCCTGGGCCATa < 1:881142/150‑1 (MQ=255) ttGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGcggcgg > 1:718025/1‑83 (MQ=255) ttGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGcggcgg < 2:718025/83‑1 (MQ=255) ttGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGACGCACCTGGCTCAGCAACCACTCGCGCCCTGGGCCATAGATa > 1:514371/1‑150 (MQ=255) ccATCAGCATCGGCCTGCAGCAGAAACGCCAGCGGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGACGCACCTGGCTCAGCaa < 2:157076/122‑1 (MQ=255) ccATCAGCATCGGCCTGCAGCAGAAACGCCAGCGGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGACGCACCTGGCTCAGCaa > 1:157076/1‑122 (MQ=255) cAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGACGCACCTGGCTCAGCAACCACTCGCGCCCTGGGCCATAGATACCGGTCa < 2:951802/150‑1 (MQ=255) cATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGACGCACCTGGCTCAGCAACCACTCGCGCCCTGGGCCATAGATACCGGTCAGGc < 1:949705/150‑1 (MQ=255) aTCGGCCTGCAGCAGAAACGCCAGTAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCAGACAGCGGCGGCTCCTCGGCAACGCGGTAACTCTGACGCACCTGGCTCAGCAACCACTCGCGCCCTGGGCCATAGATACCGGTCAGGCg < 2:111797/150‑1 (MQ=255) cTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGACTCACCTGGCTCAGCAACCACTCGCGCCCTGGGCCATAGATACCGGTCAGGCGGACCAc > 2:232269/1‑150 (MQ=255) tgccGCCAGCCTGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGACGCACCTGGCTCAGCAACCACGCTCGCCCTTGGCCATAGATACCGGTCAGGCGGACCACGCTCGCCgg > 2:678320/4‑150 (MQ=255) cagcagGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGACGCACCTGGCTCAGCAACCACTCGCGCCCTGGGCCATAGATACCGGTCAGGCGGACCACGCTCGCCGGGATGcc > 2:999716/1‑150 (MQ=255) agGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGACGCACCTGGCTCAGCAACCACTCGCGCCCTGGGCCATAGATACCGGTCAGGCGGACCACGCTCGCCGGGATGCCGCTg < 1:599051/150‑1 (MQ=255) | TCATCGGACCATTCGGTAACCCCCATGTAGGCCCGCAGCCAGGCCACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGACGCACCTGGCTCAGCAACCACTCGCGCCCTGGGCCATAGATACCGGTCAGGCGGACCACGCTCGCCGGGATGCCGCTG > NC_002947/6052789‑6053079 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |