Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 557,000 A→G 8.5% T16A (ACG→GCG)  rpsN → 30S ribosomal protein S14

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947557,0000AG 8.5% 129.7 / 2.9 47T16A (ACG→GCG) rpsN30S ribosomal protein S14
Reads supporting (aligned to +/- strand):  ref base A (29/14);  new base G (2/2);  total (31/16)
Fisher's exact test for biased strand distribution p-value = 5.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.63e-01

GTTTGGACATCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTCGCGCCTGCGCAACCGTTGCCGC  >  NC_002947/556853‑557149
                                                                                                                                                   |                                                                                                                                                     
gTTTGGACATCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACg                                                                                                                                                     <  2:722986/150‑1 (MQ=255)
          cACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAgg                                                                                                                                           >  1:925181/1‑149 (MQ=255)
            cccTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTc                                                                                                                                         >  2:266812/1‑150 (MQ=255)
             ccTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGTGAGCTGACGCTTCAGCTCGCGGtttttcat                                                                                                                                            <  1:809289/146‑8 (MQ=38)
             ccTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGTGAGCTGACGCTTCAGCTCGCGGtttttcat                                                                                                                                            >  2:809289/1‑139 (MQ=38)
                         tGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTg                                                                                                                            >  1:905293/1‑150 (MQ=255)
                             cGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTgctga                                                                                                                        <  1:1026526/150‑1 (MQ=255)
                               ttCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGTGAGCTGACGCTTCAGCTCGCGGtttttc                                                                                                                                              <  2:488586/126‑6 (MQ=38)
                               ttCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGTGAGCTGACGCTTCAGCTCGCGGtttttc                                                                                                                                              >  1:488586/1‑121 (MQ=38)
                                  ggACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTgctgagctga                                                                                                                   >  1:221479/1‑150 (MQ=255)
                                   gacgacGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGaa                                                                                                                  >  1:1025710/1‑150 (MQ=255)
                                    acgacgAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGaaa                                                                                                                 <  1:877159/150‑1 (MQ=255)
                                      gacgaAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGc                                                                                                               >  1:48575/1‑150 (MQ=255)
                                               cgcgCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGt                                                                                                      <  1:738030/150‑1 (MQ=255)
                                               cgcgCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTGCGCTAAGAAGCGGGCTGAGCTGAAAGCGACCATCgc                                                                                                      >  2:738030/1‑149 (MQ=255)
                                                        ctgcGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGaa                                                                                             <  2:221479/150‑1 (MQ=255)
                                                                  tCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACg                                                                                                                                                     >  2:844144/1‑84 (MQ=255)
                                                                  tCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACg                                                                                                                                                     <  1:844144/84‑1 (MQ=255)
                                                                                 gCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGt                                                                                                                                           >  1:785372/1‑79 (MQ=255)
                                                                                 gCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGt                                                                                                                                           <  2:785372/79‑1 (MQ=255)
                                                                                  cAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTg                                                                   >  1:147617/1‑150 (MQ=255)
                                                                                                 ggAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCCGc                                                    >  1:580815/1‑150 (MQ=255)
                                                                                                  gAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAAc                                                                                                  <  2:420450/103‑1 (MQ=255)
                                                                                                  gAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAAc                                                                                                  >  1:420450/1‑103 (MQ=255)
                                                                                                    aaaTGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGa                                                 <  2:1025710/150‑1 (MQ=255)
                                                                                                          ccAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAagcagc                                           >  1:322296/1‑150 (MQ=255)
                                                                                                           cAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGa                                                                                                             >  1:766217/1‑83 (MQ=255)
                                                                                                           cAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGa                                                                                                             <  2:766217/83‑1 (MQ=255)
                                                                                                            aagaagAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCa                                         >  2:37430/1‑150 (MQ=255)
                                                                                                                    cATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGc                                 >  1:1000555/1‑150 (MQ=255)
                                                                                                                    cATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGc                                 >  1:522465/1‑150 (MQ=255)
                                                                                                                    cATGAAAAACCGCGAGCTGAAGCGTAAGCTCACGGTAGCAAAGTTCGCAAAGAATCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGc                                 >  2:431581/1‑150 (MQ=255)
                                                                                                                      tGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTa                               >  1:454918/1‑150 (MQ=255)
                                                                                                                        aaaaaCCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAgc                             <  1:266812/150‑1 (MQ=255)
                                                                                                                         aaaaCCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGCGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAgcg                            >  2:448884/1‑150 (MQ=255)
                                                                                                                         aaaaCCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGa                                    >  2:392495/1‑142 (MQ=255)
                                                                                                                         aaaaCCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGa                                    <  1:392495/142‑1 (MQ=255)
                                                                                                                              cgcgAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTcg                       >  2:133499/1‑150 (MQ=255)
                                                                                                                                cgAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTcgcg                     >  2:742945/1‑150 (MQ=255)
                                                                                                                                   gCTGAAGCGTCAGCTCACGGTAGCAAAGTTCTCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCCGCAGAAGCAGCCACGCGACGCTAGCGCCTCGCGCCt                  <  2:580815/150‑1 (MQ=255)
                                                                                                                                     tGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTCGCGCCTgc                >  1:726770/1‑150 (MQ=255)
                                                                                                                                     tGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGCTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTcgc                      >  1:708330/1‑144 (MQ=255)
                                                                                                                                     tGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGCTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTcgc                      <  2:708330/144‑1 (MQ=255)
                                                                                                                                           gTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGACGTTGTCGCACTGACAATGAATCATCGCCACGCTAGCGCCGCGCGCCTGCGCAAcc          >  2:966727/1‑150 (MQ=255)
                                                                                                                                                cTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTCGCGCCTGCGCAACCGTTgc     >  1:505785/1‑150 (MQ=255)
                                                                                                                                                 tCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGTCACGCGACGCTAGCGCCTCGCGCCTGCGCAACCGTTgcc    >  1:602561/1‑150 (MQ=255)
                                                                                                                                                 tCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTCGCGCCTGCGCAACCGTTgcc    >  1:795690/1‑150 (MQ=255)
                                                                                                                                                   aCGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTCGCGCCTGCGCAACCGTTgccgc  >  2:480081/1‑150 (MQ=255)
                                                                                                                                                   aCGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTCGCGCCTGCGCAACCGTTgccgc  <  1:133499/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GTTTGGACATCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTCGCGCCTGCGCAACCGTTGCCGC  >  NC_002947/556853‑557149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑