| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 578,044 | C→A | 8.7% | Q4K (CAA→AAA) | fdoH → | formate dehydrogenase‑O subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 578,044 | 0 | C | A | 8.7% | 138.3 / 2.1 | 46 | Q4K (CAA→AAA) | fdoH | formate dehydrogenase‑O subunit beta |
| Reads supporting (aligned to +/- strand): ref base C (21/21); new base A (2/2); total (23/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01 | |||||||||||
ATCGGCATTCCGCTGCACTGGGGCTTCACCGGCGCTACCCGGCATGGCTACCTGACCAATACCTTGGTGCCGTTCCTCGGTGACGGCAACACGCAGACGCCAGAGTCCAAGTCGTTCCTCGTGAAAGTGGAGAAACTCTGATGGCCAGCCAAGACATCATCGCCCGCTCGGCCACCACTACCGTGCCGCCCTCGGTGCGTCAGCAGGAGGAGGTCGCCAAGCTGATCGACACTACCAAGTGCATCGGCTGCAAGGCCTGCCAGGTGGCGTGCTCGGAGTGGAATGAACTGCGTGACG > NC_002947/577895‑578191 | aTCGGCATTCCGCTGCACTGGGGCTTCACCGGCGCTACCCGGCATGGCTACCTGACCAATACCTTGGTGCCGTTCCTCGGTGACGGCAACACGCAGACGCCAGAGTCCAAGTCGTTCCCCGTGAAAGTGGAGAAACTCTAATGgccagcc > 1:292605/1‑150 (MQ=255) gCATTCCGCTGCACTGGGGCTTCACCGGCGCTACCCGGCATGGCTACCTGACCAATACCTTGGTGCCGTTCCTCGGTGACGGCAACACGCAGACGCCAGAGTCCAAGTCGTTCCTCGTGAAAGTGGAGAAACTCTGATGGCCAGCCAAGa > 2:8755/1‑150 (MQ=255) cATTCCGCTGCACTGGGGCTTCACCGGCGCTACCCGGCATGGCTACCTGACCAATACCTTGGTGCCGTTCCTCGGTGACGGCAACACGCAGACGCCAGAGTCCAAGTCGTTCCTCGTGAAAGTGGAGAAACTCTGATGGCCAGCCAAGAc < 1:858554/150‑1 (MQ=255) cGCTGCACTGGGGCTTCACCGGCGCTACCCGGCATGGCTACCTGACCAATACCTTGGTGCCGTTCCTCGGTGACGGCAACACGCAGACGCCAGAGTCCAAGTCGTTCCTCGTGAAAGTGGAGAAACTCTGATGGCCAGCCAAGAcatcat > 1:491945/1‑150 (MQ=255) gCTGCACTGGGGCTTCACCGGCGCTACCCGGCATGGCTACCTGACCAATACCTTGGTGCCGTTCCTCGGTGACGGCAACACGCAGACGCCAGAGTCCAAGTCGTTCCTCGTGAAAGTGGAGAAACTCTGATGGCCAGCCAAGACatcatc < 1:863241/150‑1 (MQ=255) tGCACTGGGGCTTCACCGGCGCTACCCGGCATGGCTACCTGACCAATACCTTGGTGCCGTTCCTCGGTGACGGCAACACGCAGACGCCAGAGTCCAAGTCGTTCCTCGTGAAAGTGGAGAAACTCTGATGGCCAGCCAAGACATCATCGc < 1:1084164/150‑1 (MQ=255) cggggCCTCACCGGCGCTACCCGGCATGGCTAACTGACCAATACCTTGGTGCCGTTCCTCGGTGACGGCAACACGCACACACCAGAGTCCAAGTCGTTCCTCGTGAAAGTGGAGAAACTCTGATGGCCAGCCAAGACATCATCGCCCGCt > 2:443722/2‑150 (MQ=255) cATGGCTACCTGACCAATACCTTGGTGCCGTTCCTCGGTGACGGCAACACGCAGACGCCAGAGTCCAAGTCGTTCCTCGTGAAAGTGGAGAAACTCTGATGGCCAGCCAAGACATCATCGCCCGCTCGGCCACCACTACCGTGCCGCCCt < 1:635008/150‑1 (MQ=255) tGGCTACCGGACCAATACCTTGGTGCCGTTCCTCGGTGACAGCAACACGCAGACGCCAGAGTCCAAGTCGTTCCTCGTGAAAGTGGAGAAACTCTGATGGCCAGCCAAGACATCATCGCCCGCTCGGCCACCACTACCGTGCCGCCCTCg < 2:841296/150‑1 (MQ=255) aCCAATACCTTGGTGCCGTTCCTCGGTGACGGCAACACGCAGACGCCAGAGTCCAAGTCGTTCCTCGTGAAAGTGGAGAAACTCTGATGGCCAGCCAAGACATCATCGCCCGCTCGGCCACCACTACCGt < 1:39212/130‑1 (MQ=255) aCCAATACCTTGGTGCCGTTCCTCGGTGACGGCAACACGCAGACGCCAGAGTCCAAGTCGTTCCTCGTGAAAGTGGAGAAACTCTGATGGCCAGCCAAGACATCATCGCCCGCTCGGCCACCACTACCGt > 2:39212/1‑130 (MQ=255) aTACCTTGGTGCCGTTCCTCGGTGACGGCAACACGCAGACGCCAGAGTCCAAGTCGTTCCTCGTGAAAGTGGAGAAACTCTGATGGCCAGCCAAGACATCATCGCCCGCTCGGCCACCACTACCGTGCCGCCCTCGGTGCGTCAGCagga < 1:587311/150‑1 (MQ=255) ttGGTGCCGTTCCTCGGTGACGGCAACACGCAGACGCCAGAGTCCAAGTCGTTCCTCGTGAAAGTGGAGAAACTCTGATGGCCAGCCAAGACATCATCGCCCGCTCGGCCACCACTACCGTGCCGCCCTCGGTGCGTCCGCaggaggagg > 1:27563/1‑150 (MQ=255) ggTGCCGTTCCTCGGTGACGGCAACACGCAGACGCCAGAGTCCAAGTCGTTCCTCGTGAAAGTGGAGAAACTCTGATGGCCAGCCAAGACATCATCGCCCGCTCGGCCACCACTACCGTGCCGCCCTCGGTGCGTCAGCAGGAGGAGGTc < 2:424467/150‑1 (MQ=255) ggTGCCGTTCCGCGGTGACGGCAACACGCAGACGCCAGAGTCCAAGTCGTTCCTCGTGAAAGTGGAGAAACTCTGATGGCCAGCCAAGACATCATCGCCCGCTCGGCCACCACTACCGTGACGCCCTCGGTGCGTCAGCAGGAGGAGGTc < 2:468666/150‑1 (MQ=255) ttCCTCGGTGACGGCAACACGCAGACGCCAGAGTCCAAGTCGTTCCTCGTGAAAGTGGAGAAACTCTGATGGCCAGCCAAGACATCATCGCCCGCTCGGCCACCACTACCGTGCCGCCCTCGGTGCGTCAGCAGGAGGAGGTCGCCAAGc < 1:146577/150‑1 (MQ=255) tCCTCGGTGACGGCAACACGCAGACGCCAGAGTCCAAGTCGTTCCTCGTGAAAGTGGAGAAACTCTGATGGCCAGCCAAGACATCATCGCCCGCTCGGCCACCACTACCGTGCCGCCCTCGGTGCGTCAGCAGGAGGAGGTCGCCAAGCt < 2:179584/150‑1 (MQ=255) ggCACCACGCAGACGCCAGAGTCCAAGTCGTTCCTCGTGAAAGTGGAGAAACTCTGATGGCCAGCCAAGACATCATCGCCCGCTCGGCCACCACTACCGTGCCGCCCTCGGTGCGTCAGCAGGAGGAGGTCGCCAAGCTGATCGACACTa < 2:139845/150‑1 (MQ=255) ggCAACACGCAGACGCCAGAGTCCAAGTCGTTCCTCGTGAAAGTGGAGCAACTCTGATGGCCAGCCAAGACATCATCGCCCGCTCGGCCACCACTACCGTGCCGCCCTCGGTGCGTCAGCAGGAGGAGGTCGCCAAGCTGATCGACACTa > 1:202431/1‑150 (MQ=255) acacGCAGACGCCAGAGTCCAAGTCGTTCCTCGTGAAAGTGGAGAAACTCTGATGGCCAGCCAAGACATCATCGCCCGCTCGGCCACCACTACCg < 2:957344/95‑1 (MQ=255) acacGCAGACGCCAGAGTCCAAGTCGTTCCTCGTGAAAGTGGAGAAACTCTGATGGCCAGCCAAGACATCATCGCCCGCTCGGCCACCACTACCg > 1:957344/1‑95 (MQ=255) acacGCAGACGCCAGAGTCCAAGTCGTTCCTCGTGAAAGTGGAGAAACTCTGATGGCCAGCCAAGACATCATCGCCCGCTCGGCCACCACTACCGTGCCGCCCTCGGTGCGTCAGCAGGAGGAGGTCGCCAAGCTGATCGACACTACCaa < 1:8755/150‑1 (MQ=255) cAGACGCCAGAGTCCAAGTCGTTCCTCGTGAAAGTGGAGAAACTCTGATGGCCAGCCAAGACATCATCGCCCGCTCGGCCACCACTACCGTGCCGCCCTCGGTGCGTCAGCAGGAGGAGGTCGCCAAGCTGATCGACACTACCAAGTGCa > 1:572061/1‑150 (MQ=255) aGACGCCAGAGTCCAAGTCGTTCCTCGTGAAAGTGGAGAAACTCTGATGGCCAGCCAAGACATCATCGCCCGCTCGGCCACCACTACCGTGCCGCCCTCGGTGCGTCAGCAGGAGGAGGTCGCCAAGCTGATCGACACTACCAAGTGCAt > 2:1085206/1‑150 (MQ=255) gCCAGAGTCCAAGTCGTTCCTCGTGAAAGTGGAGAAACTCTGATGGCCAGCCAAGACATCATCGCCCGCTCGGCCACCACTACCGTGCCGCCCTCGGTGCGTCAGCAGGAGGGGGACGGCAAGCTGATCGACACTACCAAGTGCATCGGc > 2:640565/1‑150 (MQ=255) gCCAGAGTCCAAGTCGTTCCTCGTGAAAGTGGAGAAACTCTGATGGCCAGCCAAGACATCATCGCCCGCTCGGCCACCACTACCGTGCCGCCCTCGGTGCGTCAGCAGGAGGAGGTc > 1:1064816/1‑117 (MQ=255) gCCAGAGTCCAAGTCGTTCCTCGTGAAAGTGGAGAAACTCTGATGGCCAGCCAAGACATCATCGCCCGCTCGGCCACCACTACCGTGCCGCCCTCGGTGCGTCAGCAGGAGGAGGTc < 2:1064816/117‑1 (MQ=255) gCCAGAGTCCAAGTCGTTCCTCGTGAAAGTGGAGAAACTCTGATGGCCAGCCAAGACATCATCGCCCGCTCGGCCACCACTACCGTGCCGCCCTCGGTGCGTCAGCAGGAGGAGGTCGCCAAGCTGATCGACACTACCAAGTGCATCGGc < 2:491945/150‑1 (MQ=255) cAGAGTCCAAGTCGTTCCTCGTGAAAGTGGAGAAACTCTGATGGCCAGCCAAGACATCATCGCCCGCTCGGCCACCACTACCGTGCCGCCCTCGGTGCGTCAGCAGGAGGAGGTCGCCAAGCTGATCGACACTACCAAGTGCATCGGCTg > 1:414381/1‑150 (MQ=255) cAGAGTCCAAGTCGTTCCTCGTGAAAGTGGAGAAACTCTGATGGCCAGCCAAGACATCATCGCCCGCTCGGCCACCACTACCGTGCCGCCCTCGGTGCGTCAGCAGGAGGAGGTCGCCAAGCTGATCGACACTACCAAGTGCATCGGCTg > 1:128778/1‑150 (MQ=255) aaGTCGTTCCTCGTGAAAGTGGAGAAACTCTGATGGCCAGCCAAGACATCATCGCCCGCTCGGCCACCACTACCGTGCCGCCCTCGGTGCGTCAGCAGGAGGAGGTCGCCAAGCTGATCGACACTACCAAGTGCATCGGCTGCAAGGCCt > 2:552070/1‑150 (MQ=255) tCGTTCCTCGTGAAAGTGGAGAAACTCTGATGGCCAGCCAAGACATCATCGCCCGCTCGGCCACCACTACCGTGCCGCCCTCGGTGCGTCAGCAGGAGGAGGTCGCCAAGCTGATCGACACTACCAAGTGCATCGGCTGCAAGGCCTGcc > 2:199717/1‑150 (MQ=255) ttCCTCGTGAAAGTGGAGAAACTCTGATGGCCAGCCAAGACATCATCGCCCGCTCGGCCACCACTACCGTGCCGCCCTCGGTGCGTCAGCAGGAGGAGGTCGCCAAGc > 1:916510/1‑108 (MQ=255) ttCCTCGTGAAAGTGGAGAAACTCTGATGGCCAGCCAAGACATCATCGCCCGCTCGGCCACCACTACCGTGCCGCCCTCGGTGCGTCAGCAGGAGGAGGTCGCCAAGc < 2:916510/108‑1 (MQ=255) tCGTGAAAGTGGAGAAACTCTGATGGCCAGCCAAGACATCATCGCCCGCTCGGCCACCACTACCGTGCCGCCCTCGGTGCGTCAGCAGGAGGAGGTCTCCAAGCTGATCGACACTACCAAGTGCATCGGCTGCAAGGCCTGCCAGGTGGc < 2:1122229/150‑1 (MQ=255) tCGTGAAAGTGGAGAAACTCTGATGGCCAGCCAAGACATCATCGCCCGCTCGGCCACCACTACCGTGCCGCCCTCGGTGCGTCAGCAGGAGGAGGTCGCCAAGCTGATCGACACTACCAAGTGCATCGGCTGCAAGGCCTGCCAGGTGGc > 1:1122229/1‑150 (MQ=255) gTGAAAGTGGAGAAACTCTGATGGCCAGCCAAGACATCATCGCCCGCTCGGCCACCACTACCGTGCCGCCCTCGGTGCGTCAGCAGGAGGAGGTCGCCAAGCTGATCGACACTACCAAGTGCATCGGCTGCAAGGCCTGCCAGGTGGCgg > 1:549435/1‑149 (MQ=255) aaaGTGGAGAAACTCTGATGGCCAGCCAAGACATCATCGCCCGCTCGGCCACCACTACCGTGCCGCCCTCGGTGCGTCAGCAGGAGGAGGTCGCCAAGCTGATCGACACTACCAAGTGCATCGGCTGCAAGGCCTGCCAGGTGGCGTGCt > 1:100843/1‑150 (MQ=255) tGGAGAAACTCTGATGGCCAGCCAAGACATCATCGCCCGCTCGGCCACCACTACCGTGCCGCCCTCGGTGCGTCAGCAGGAGGAGGTCGCCAAGCTGATCGACACTACCAAGTGCATCGGCTGCAAGGCCTGCCAGGTGGCGTGCTCGGa < 2:100843/150‑1 (MQ=255) aaaCTCTGATGGCCAGCCAAGACATCATCGCCCGCTCGGCCACCACTACCGTGCCGCCCTCGGTGCGTCAGCAGGAGGAGGTCGCCAAGCTGATCGACACTACCAAGTGCATCGGCTGCAAGGCCTGCCAGGTGGCGTGCTCGGAGTGGa > 1:173677/1‑150 (MQ=255) aaaCTCTGATGGCCAGCCAAGACATCATCGCCCGCTCGGCCACCACTACCGTGCCGCCCTCGGTGCGTCAGCAGGAGGAGGTCGCCAAGCTGATCGACACTACCAAGTGCATCGGCTGCAAGGCCTGCCAGGTGGCGTGCTCGGAGTGGa > 2:9022/1‑150 (MQ=255) aCTCTGATGGCCAGCCAAGACATCATCGCCCGCTCGGCCACCACTACCGTGCCGCCCTCGGTGCGTCAGCAGGAGGAGGTCGCCAAGCTGATCGACACTACCAAGTGCATCGGCTGCAAGGCCTGCCAGGTGGCGTGCTCGGAGTGGAAt < 2:173677/150‑1 (MQ=255) ctGATGGCCAGCCAAGACATCATCGCCCGCTCGGCCACCACTACCGTGCCGCCCTCGGTGCGTCAGCAGGAGGAGGTCGCCAAGCTGATCGACACTACCAAGTGCATCGGCTGCAAGGCCTGCCAGGTGGCGTGCTCGGAGTGTAATGaa < 1:1095158/150‑1 (MQ=255) tGATGGCCAGCAAAGACATCATCGCCCGCTCGGCCACCACTACCGTGCCGCCCTCGGTGCGTCAGCAGGAGGAGGTCGCCAAGCTGATCGACACTACCAAGTGCATCGGCTGCAAGGCCTGCCAGGTGGCGTGCTCg < 2:958823/137‑1 (MQ=255) tGATGGCCAGCAAAGACATCATCGCCCGCTCGGCCACCACTACCGTGCCGCCCTCGGTGCGTCAGCAGGAGGAGGTCGCCAAGCTGATCGACACTACCAAGTGCATCGGCTGCAAGGCCTGCCAGGTGGCGTGCTCg < 2:961975/137‑1 (MQ=255) tGATGGCCAGCAAAGACATCATCGCCCGCTCGGCCACCACTACCGTGCCGCCCTCGGTGCGTCAGCAGGAGGAGGTCGCCAAGCTGATCGACACTACCAAGTGCATCGGCTGCAAGGCCTGCCAGGTGGCGTGCTCg > 1:961975/1‑137 (MQ=255) tGATGGCCAGCAAAGACATCATCGCCCGCTCGGCCACCACTACCGTGCCGCCCTCGGTGCGTCAGCAGGAGGAGGTCGCCAAGCTGATCGACACTACCAAGTGCATCGGCTGCAAGGCCTGCCAGGTGGCGTGCTCg > 1:958823/1‑137 (MQ=255) cagccaAGACATCATCGCCCGCTCGGCAACCACTACCGTGCCGCCCTCGGTGCGTCAGCAGGAGGAGGTCGCCAAGCTGATCGACACTACCAAGTGCATCGGCTGCAAGGCCTGCCAGGTGGCGTGCTCGGAGTGGAATGAACTGCGTGa > 2:404229/1‑150 (MQ=255) gccaAGACATCATCGCCCGCTCGGCCACCACTACCGTGCCGCCCTCGGTGCGTCAGCAGGAGGAGGTCGCCAAGCTGATCGACACTACCAAGTGCATCGGCTGCAAGGCCTGCCAGGTGGCGTGCTCGGAGTGGAATGAACTGCGTGACg > 2:264960/1‑150 (MQ=255) | ATCGGCATTCCGCTGCACTGGGGCTTCACCGGCGCTACCCGGCATGGCTACCTGACCAATACCTTGGTGCCGTTCCTCGGTGACGGCAACACGCAGACGCCAGAGTCCAAGTCGTTCCTCGTGAAAGTGGAGAAACTCTGATGGCCAGCCAAGACATCATCGCCCGCTCGGCCACCACTACCGTGCCGCCCTCGGTGCGTCAGCAGGAGGAGGTCGCCAAGCTGATCGACACTACCAAGTGCATCGGCTGCAAGGCCTGCCAGGTGGCGTGCTCGGAGTGGAATGAACTGCGTGACG > NC_002947/577895‑578191 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |