Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 593,436 G→T 7.1% E391* (GAG→TAG) ‡ PP_0503 → MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947593,4360GT 7.1% 220.2 / 4.8 70E391* (GAG→TAG) ‡PP_0503MFS transporter
Reads supporting (aligned to +/- strand):  ref base G (32/33);  new base T (2/3);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.90e-01

TATTCGCTGGGCAGCGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGTGTCGATGTCGACGTACCA  >  NC_002947/593292‑593584
                                                                                                                                                |                                                                                                                                                    
tATTCGCTGGGCAGCGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGgcc                                                                                                                                                 >  2:1038204/1‑150 (MQ=255)
  ttCNCTGGGCAGCGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGgccgc                                                                                                                                               >  2:696556/1‑150 (MQ=255)
  ttCGCTGGGCAGCGTTTTGGGTGCGGGGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGgccgc                                                                                                                                               >  2:856147/1‑150 (MQ=255)
    cGCTGGGCAGCGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCTCGGCCGccc                                                                                                                                             <  2:1027083/150‑1 (MQ=255)
    cGCTGGGCAGCGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGccc                                                                                                                                             <  2:938203/150‑1 (MQ=255)
     gCTGGGCAGCGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCt                                                                                                                                            >  1:39636/1‑150 (MQ=255)
          gCAGCGGTTTGGGGGCGGTGTTTGCGAGCTGGGTGGTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCTGCCGGAGTGTGCTACTGGCTATAGCTGCAACGCGCGGGGGTGGCCGAGGATGTCCTGCAAt                                                                                                                                       <  2:332148/150‑1 (MQ=255)
            aGCGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGt                                                                                                                                     <  1:316213/150‑1 (MQ=255)
              cGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTc                                                                                                                                   >  2:611135/1‑150 (MQ=255)
                gTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTTTAATGTTCAg                                                                                                                                 <  2:515485/150‑1 (MQ=255)
                gTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAg                                                                                                                                 <  2:897723/150‑1 (MQ=255)
                gTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAg                                                                                                                                 <  2:1023172/150‑1 (MQ=255)
                 tttGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAgg                                                                                                                                <  1:746399/150‑1 (MQ=255)
                           gtgtTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTg                                                                                                                      <  1:398563/150‑1 (MQ=255)
                                  cGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGCCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGTGCTAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCaa                                                                                                               <  1:631454/150‑1 (MQ=255)
                                         ggTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGt                                                                                                                     <  2:513525/137‑1 (MQ=255)
                                         ggTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGt                                                                                                                     >  1:513525/1‑137 (MQ=255)
                                           tgtTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCa                                                                                                      >  2:499018/1‑150 (MQ=255)
                                            gtTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCAt                                                                                                     >  1:256613/1‑150 (MQ=255)
                                             tttGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATg                                                                                                    <  2:256613/150‑1 (MQ=255)
                                               tGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGta                                                                                                  <  1:856147/150‑1 (MQ=255)
                                                 cacaCTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAgaccg                                                                                                          <  2:529727/140‑1 (MQ=255)
                                                 cacaCTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAgaccg                                                                                                          >  1:529727/1‑140 (MQ=255)
                                                  acacTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCCTCAGTGCCGCCGCACTGTGCTACTGGCTATGGATGCCACGGGCGAGCGGCGCAGATCCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATAc                                                                                               >  1:523454/1‑150 (MQ=255)
                                                    acTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCATAACTTGTAGCCAAGACCGACCATGTATAccc                                                                                             >  2:1082996/1‑150 (MQ=255)
                                                    acTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATAccc                                                                                             >  1:609860/1‑150 (MQ=255)
                                                           cTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtc                                                                                      >  2:456654/1‑150 (MQ=255)
                                                           cTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtc                                                                                      <  2:548504/150‑1 (MQ=255)
                                                              ggTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtcgac                                                                                   >  2:1123574/1‑150 (MQ=255)
                                                                ttGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtcgacgt                                                                                 >  2:736338/1‑150 (MQ=255)
                                                                ttGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtcgacgt                                                                                 >  1:534742/1‑150 (MQ=255)
                                                                  gCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtcgacgtcg                                                                               >  2:366447/1‑150 (MQ=255)
                                                                    ggTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtcgacgtcgac                                                                             >  1:615041/1‑150 (MQ=255)
                                                                     gtgtgtGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGtat                                                                                                 <  2:926935/129‑1 (MQ=255)
                                                                     gtgtgtGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGtat                                                                                                 >  1:926935/1‑129 (MQ=255)
                                                                       gtgtgGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtcgacgtcgacgtc                                                                          <  1:736338/150‑1 (MQ=255)
                                                                          tgGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtcgacgtcgacgtcgac                                                                       <  1:456654/150‑1 (MQ=255)
                                                                          tgGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtcgacgtcgacgtcgac                                                                       >  1:271376/1‑150 (MQ=255)
                                                                            ggCNTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCt                                                                     >  2:700671/1‑150 (MQ=255)
                                                                                 ggCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTc                                                                <  1:611135/150‑1 (MQ=255)
                                                                                 ggCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGAGCGAGAGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTc                                                                >  2:1122098/1‑150 (MQ=255)
                                                                                    aTGGGCAGCAGTGCCGTCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTg                                                             <  2:730767/150‑1 (MQ=255)
                                                                                     tGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGc                                                            <  1:818190/150‑1 (MQ=255)
                                                                                        gCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCTACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGt                                                         <  2:271376/150‑1 (MQ=255)
                                                                                        gCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGt                                                         <  2:534742/150‑1 (MQ=255)
                                                                                           tCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGc                                                      >  2:989513/1‑150 (MQ=255)
                                                                                           acAGTGCGGCGGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGc                                                      >  1:945291/2‑150 (MQ=255)
                                                                                              gTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCaa                                                   >  2:428979/1‑150 (MQ=255)
                                                                                               tGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAg                                                  >  1:660355/1‑150 (MQ=255)
                                                                                                gccgccGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAgg                                                 <  1:1038204/150‑1 (MQ=255)
                                                                                                   gccgcACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACtt                                                                                                                       <  2:512467/77‑1 (MQ=255)
                                                                                                   gccgcACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACtt                                                                                                                       >  1:512467/1‑77 (MQ=255)
                                                                                                        aCTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCgg                                         <  2:866997/150‑1 (MQ=255)
                                                                                                          tgtgCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCTACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGc                                       >  2:1112084/1‑150 (MQ=255)
                                                                                                          tgtgCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGACGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGc                                       >  2:234243/1‑150 (MQ=255)
                                                                                                                 cTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtcgac                                                                                   >  2:529784/1‑99 (MQ=255)
                                                                                                                 cTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtcgac                                                                                   <  1:529784/99‑1 (MQ=255)
                                                                                                                       aTGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGc                          >  2:819281/1‑150 (MQ=255)
                                                                                                                           cTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTg                      <  2:39636/150‑1 (MQ=255)
                                                                                                                              cAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACgg                                                                                         <  2:208566/80‑1 (MQ=255)
                                                                                                                              cAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACgg                                                                                         >  1:208566/1‑80 (MQ=255)
                                                                                                                               aaCGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGgtgt                  <  1:499018/150‑1 (MQ=255)
                                                                                                                                   cgcgAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATCCCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCACGGGCGGTCGGGCCGTTGACGCTGGCCTTGGtgtcgat              <  1:366447/150‑1 (MQ=255)
                                                                                                                                   cgcgAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGtgtcgat              <  1:1123574/150‑1 (MQ=255)
                                                                                                                                     cgAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGtgtcgatgt            <  1:700671/150‑1 (MQ=255)
                                                                                                                                       aGGGCGGCCTCGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCAc                                                                                           >  2:54761/1‑69 (MQ=39)
                                                                                                                                       aGGGCGGCCTCGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCAc                                                                                           <  1:54761/69‑1 (MQ=39)
                                                                                                                                       aGGGCGGCCTCGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACgg                                                                                         <  1:887729/71‑1 (MQ=39)
                                                                                                                                       aGGGCGGCCTCGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACgg                                                                                         >  2:887729/1‑71 (MQ=255)
                                                                                                                                               cGAGCCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGTGTCGATGTCGACGTACCa  <  2:523454/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
TATTCGCTGGGCAGCGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGTGTCGATGTCGACGTACCA  >  NC_002947/593292‑593584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑