Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,544,5430GC35.5% 190.2 / 182.0 166intergenic (+60/+54)mdfA/ampGmultidrug MFS transporter/muropeptide permease AmpG
Reads supporting (aligned to +/- strand):  ref base G (46/61);  new base C (27/32);  total (73/93)
Fisher's exact test for biased strand distribution p-value = 7.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.29e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 177 total aligned reads displayed.

TGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCGATCAGGATCAGCGTAGGCA  >  NC_002947/1544395‑1544691
                                                                                                                                                     |                                                                                                                                                           
tGGCGGGGGGGGGGGGGGGGGGGTGGGCGGGGTGGCCGGGGTGGTTTGGGGGGGGGGGGCGGCCTGGGG‑AAGGGGCTGGAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCCAGGgccgcgccgc                                                                                                                                                            <  1:2151650/150‑1 (MQ=37)
 ggggggggggggggCGGGGGGGTGGGGGCTGGGGCCGCGGGGGTTTGGGGTGGGGGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCt                                                                                                                                                           <  1:2176868/144‑1 (MQ=38)
     gcggGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCCTgtgt                                                                                                                                                       <  2:865141/150‑1 (MQ=25)
     gcggGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCCTgtgt                                                                                                                                                       <  2:4540476/150‑1 (MQ=37)
     gcggGGGGGGGGGGGGCGTGGGCGCTGGGGCCGCTGTGGTTTGGGGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGt                                                                                                                                                       <  1:738258/150‑1 (MQ=39)
      gggggggggggggggCGTTGGCGCGGTGGCCGCTGTGGTTTGGGGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCCTGTGTa                                                                                                                                                      <  1:713108/149‑1 (MQ=25)
      gggggggggggggggCGTGGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCCTGTGTa                                                                                                                                                      <  2:4979240/149‑1 (MQ=21)
      gggggggggCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCAATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCCTGTGTa                                                                                                                                                      <  2:4245464/149‑1 (MQ=37)
      ggggggTGGGGGGGGCGTTGGCGCTGTGGCCGGTGTGTTTTGTGGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCCTGTGTa                                                                                                                                                      <  2:197036/149‑1 (MQ=25)
      ggggggTGGGGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTa                                                                                                                                                      <  1:4620937/149‑1 (MQ=255)
      ggggggTGGGGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCCTGTGTa                                                                                                                                                      <  2:1445527/149‑1 (MQ=37)
      ggggggTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTa                                                                                                                                                      <  2:4464353/149‑1 (MQ=255)
      ggggggTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCAATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCCTGTGTa                                                                                                                                                      <  2:1680257/149‑1 (MQ=37)
       ggggggggggggggCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCCTGTGTAg                                                                                                                                                     <  1:3440997/150‑1 (MQ=37)
       ggggggggggggggCGTTGGCGCGGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAg                                                                                                                                                     <  1:1690085/150‑1 (MQ=255)
       ggggggggggggggCGTGGGCGCTGTGGCCGCTGTGGTTTGTGGTGGTGGGGGGGCATGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCCTGTGTAg                                                                                                                                                     <  1:4201201/150‑1 (MQ=21)
       ggggggggCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCCTGTGTAg                                                                                                                                                     <  1:4641567/150‑1 (MQ=37)
       ggggggggCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGGAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAg                                                                                                                                                     <  1:2841949/150‑1 (MQ=255)
          gggggCGGGGGCGTGGGCGCTGTGGCCGCTGTGGTTGGTGGGGGGGGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAg                                                                                                                                                  <  2:2251241/147‑1 (MQ=39)
           gTGGCGGGGGCGTGGGCGCTGGGGCCGCTGTGGTTTGTGGTGGGGGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGc                                                                                                                                                 <  1:4363534/150‑1 (MQ=25)
             ggggggggCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCgg                                                                                                                                               <  1:1343762/147‑1 (MQ=21)
             ggggggggCGTGGGCGCTGTGGCCGCTGTGGTTTGGGGGGGGTGGGCGGCCGGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCgg                                                                                                                                               <  1:4008402/147‑1 (MQ=21)
             ggCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTTTAGCGGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCgg                                                                                                                                               <  1:3956032/150‑1 (MQ=255)
             ggCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCgg                                                                                                                                               <  2:2915764/150‑1 (MQ=37)
             ggCGGGGGCGTTGGCGCTGTGGCCGCTGGGGTTTGGGGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCgg                                                                                                                                               <  1:1831840/150‑1 (MQ=255)
              gCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCggg                                                                                                                                              <  1:1472028/150‑1 (MQ=255)
              gCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCggg                                                                                                                                              <  2:2823274/150‑1 (MQ=37)
              gCGGGGGCGTGGGCGCGGTGGCCGCTGTGTTTTGTGGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCggg                                                                                                                                              <  1:891408/150‑1 (MQ=25)
                gggggCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGtt                                                                                                                                            <  2:1332620/150‑1 (MQ=25)
                      gTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGc                                                                                                                                                 <  1:807405/139‑1 (MQ=255)
                      gTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGc                                                                                                                                                 >  2:807405/1‑139 (MQ=255)
                      gTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCcg                                                                                                                                      <  1:3677117/150‑1 (MQ=255)
                       ttGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCgg                                                                                                                                               >  2:1339466/1‑140 (MQ=255)
                         ggCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTa                                                                                                                                                      <  2:675499/131‑1 (MQ=255)
                          gcgcTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCGCTTCGCGGGTAAAACCCCGCCAACACACGGCCCCGCCCCGGGGTAGGAGCGGGGTTAacccccca                                                                                                                                  >  2:3052348/1‑145 (MQ=255)
                           cgcgGTGGCCGCGGGGGTTTGTGGGGGGGGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGCGAag                                                                                                                                 <  2:3441741/150‑1 (MQ=18)
                             cTGTGGCCGCTGTGTTTTGTGGGGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGCGAagag                                                                                                                               <  2:2032956/150‑1 (MQ=21)
                             cTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAAACCGCTACAACAAAGGGCCGCCCCGCTTTGTAGGAGCGGGTTTACCCCCCAagag                                                                                                                               >  2:3791377/1‑150 (MQ=255)
                                 ggcggcTGTGGTTGGTGGTGGGGGGGCGGCGTGGGG‑ATGGGGCTTTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACTGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCa                                                                                                                           <  2:1514975/150‑1 (MQ=37)
                                     gCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCCCGGGTGAAAACCCACCAAAACACGGCCGCGCCGCGGTGTTGGAGCGGGTTTTACCGCCACTAGGccccccc                                                                                                                       >  2:1045133/1‑145 (MQ=255)
                                            tttGTGGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAg                                                                                                                                                     <  1:3675824/113‑1 (MQ=255)
                                              gggggggggggggCGGCGTGGGT‑ACGGGGCTGTCGCGCGATACGGGGTTTGGCGGGGGCGGGCCTCTTCGGGGGTAACCCGGCTCCAACCCCGGGCCGCGCGGCTTTGTCGGCGGGGGTTAACCCGCGAAGAGGCCAGCACAGGCAATAc                                                                                                              <  2:31108/144‑1 (MQ=255)
                                                  gggggggggCCGCCCGGGGCATGGGGCTGTCGCCCTATAATGGGTATGGCGGGGGCCGCCCTCTTGGCGGGAAACCCGGCGCCTACCCCGGGCCGGGCGGCTTTGTTGGAGGGGTTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑a‑‑a‑‑‑a                                                                                                    <  2:2119580/148‑1 (MQ=255)
                                                     ggtgggCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAAACCCCGCCTAAACAGGGCCGCGCCCCGTTGGAGGTTCGGGGTTACCCCCGAAGAGGCCAGCACAGGCAAAAA‑‑A‑‑A‑‑‑AGcgt                                                                                                >  2:1569320/1‑148 (MQ=255)
                                                                        ggggCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAAACCGCGCCAAAACCGGGCCGCGCCGCTTTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGCAAAGGCCAAAA‑‑A‑‑A‑‑‑AGCTTAAGGGCCGCTCAGCcccc                                                                              >  2:2915065/1‑150 (MQ=37)
                                                                                    cGATATCCGGTAGTGCGGGGGCCGGCCTCTTCGCGGGTAACCCCGCACCTACACCGGGCCGCGCCGCTTTGACGGCGCGGTTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTcc                                                                  <  2:1260865/150‑1 (MQ=37)
                                                                                           cgttttggCCGGGGCCGGCCTCTTCGCGGTTAAACCCGCCCCTACACAGGGCCGCGCCGCTTTGTCGGCGGGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCg                                                           <  1:1151230/143‑1 (MQ=38)
                                                                                                ttGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGt                                                                                                                                                       <  2:4120920/60‑1 (MQ=14)
                                                                                                 tGCCGGGGCCGGCCTCTTCGGGGGTAAACCCGCTCCTACACTGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTcc                                                     <  2:4862461/150‑1 (MQ=255)
                                                                                                            gccTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCt                                          >  2:3304761/1‑150 (MQ=255)
                                                                                                            gccTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCt                                          <  2:2514735/150‑1 (MQ=255)
                                                                                                            gccTCTGGGGGGGAAACCCCGCTCATACCCGGGGCCGCGCCGCTTTTTCGGCGGGTTTTAACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCt                                          <  1:2400874/150‑1 (MQ=255)
                                                                                                                  tCGCGGGTAACCCCGCGCCTACCCCGGGCCGCGCCGCTTTGTCGGAGCGGGTTTCCCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGa                                    <  2:23048/150‑1 (MQ=39)
                                                                                                                  tCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGa                                    <  2:1150702/150‑1 (MQ=255)
                                                                                                                  gcgcgGGTAACCCCGCTCCTACACAGGGCCGCGCGGCTTTGTCGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGa                                    <  2:2568369/149‑1 (MQ=255)
                                                                                                                   cgcgGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGAt                                   <  2:1016002/150‑1 (MQ=255)
                                                                                                                    gcgGTTAAACCCGCACCTACACAGGGCCGCGCCGCTTTTTAGGAGCGGTTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATg                                  <  1:1198656/150‑1 (MQ=255)
                                                                                                                         tAAACCCGCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCCGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACt                             >  1:404790/1‑150 (MQ=21)
                                                                                                                          aaccccGCTCCTACCCCGGGCCGCGCCGCTTTGTCGGCGGGGGTTAACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTg                            <  1:3155483/147‑1 (MQ=255)
                                                                                                                           accccGCGCCTACACCGGGCCGCGCCGCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGc                           <  2:1865352/148‑1 (MQ=255)
                                                                                                                            aCCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCa                          >  2:4361013/1‑150 (MQ=255)
                                                                                                                             cccGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAg                         >  1:4919775/1‑150 (MQ=255)
                                                                                                                             cccGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAg                         >  1:4179093/1‑150 (MQ=255)
                                                                                                                             cccGCCCCTCCACCGGCCGGCGCGGATTTTTCGGCGGGGTTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAg                         <  1:3041939/150‑1 (MQ=255)
                                                                                                                              ccGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGc                           <  2:2687101/147‑1 (MQ=255)
                                                                                                                              ccGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGc                           >  1:2686908/1‑147 (MQ=255)
                                                                                                                              ccGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGc                           <  2:2686908/147‑1 (MQ=255)
                                                                                                                              ccGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAgg                        >  2:4346708/1‑150 (MQ=255)
                                                                                                                              ccGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAgg                        >  1:3817544/1‑150 (MQ=255)
                                                                                                                               cGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAggg                       <  1:2852809/150‑1 (MQ=255)
                                                                                                                                gcaccTACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGc                      >  1:316906/4‑150 (MQ=37)
                                                                                                                                gCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCCGGCAATAC‑‑C‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGc                      >  1:2466017/1‑150 (MQ=21)
                                                                                                                                gCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAggg                       <  1:2605988/149‑1 (MQ=37)
                                                                                                                                gCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGc                      >  1:3539009/1‑150 (MQ=37)
                                                                                                                                gCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGc                      >  1:3223388/1‑150 (MQ=37)
                                                                                                                                gCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGc                      >  1:573383/1‑150 (MQ=37)
                                                                                                                                gCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGc                      >  2:2058687/1‑150 (MQ=37)
                                                                                                                                gCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGc                      >  1:1391519/1‑150 (MQ=37)
                                                                                                                                 cTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCCGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCg                     >  1:4317531/1‑150 (MQ=21)
                                                                                                                                  tCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCga                    >  1:667889/1‑150 (MQ=37)
                                                                                                                                  tCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCga                    >  2:2675792/1‑150 (MQ=37)
                                                                                                                                   ccTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgat                   >  2:3113840/1‑150 (MQ=255)
                                                                                                                                    cTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAg                         <  1:3104612/143‑1 (MQ=255)
                                                                                                                                    cTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatc                  >  1:1338082/1‑150 (MQ=255)
                                                                                                                                    cTACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatc                  >  2:2775950/1‑150 (MQ=37)
                                                                                                                                    cTACAAAGCGGCGCGGCCCTGGGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCCGGGCgcgc                  >  2:1094063/1‑147 (MQ=37)
                                                                                                                                     tACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatca                 >  2:5084068/1‑150 (MQ=255)
                                                                                                                                     tACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatca                 >  1:2162921/1‑150 (MQ=255)
                                                                                                                                     tACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCCGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatca                 >  1:4443811/1‑150 (MQ=25)
                                                                                                                                      acacTGGGCCGCGCGGCTTTGTCGGTGGGGTTTCACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATCGCCCTGCAGTGGcatcag                <  1:1425455/150‑1 (MQ=255)
                                                                                                                                      acacAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatcag                >  2:5013818/1‑150 (MQ=255)
                                                                                                                                      acacAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatcag                >  2:3386669/1‑150 (MQ=255)
                                                                                                                                        acaGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatcagga              >  2:636045/1‑150 (MQ=255)
                                                                                                                                        acaGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatcagga              >  2:636245/1‑150 (MQ=255)
                                                                                                                                         aagcggcgcggcccTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCCGGCAATACCGATTATCGAGCTACAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCga                    >  1:1727055/7‑150 (MQ=9)
                                                                                                                                          aGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatcaggatc            >  2:3952752/1‑150 (MQ=255)
                                                                                                                                           gggCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatcaggatca           <  1:4960887/150‑1 (MQ=255)
                                                                                                                                           gggCCGCGCCGCTTTGCAGGCGCGGGATTACCCGCGCAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCACAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACGGCAGGGCgatcaggatca           <  2:5029306/150‑1 (MQ=255)
                                                                                                                                                gcgccgcTTTGTCGGCGCGGGTTTCCCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCGATCAGGATCAGCGTa      <  2:247803/150‑1 (MQ=255)
                                                                                                                                                    cgcTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCGATCAGGATCAGCGTAGGCa  >  2:367353/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                           
TGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGG‑AAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATAC‑‑A‑‑A‑‑‑AGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCGATCAGGATCAGCGTAGGCA  >  NC_002947/1544395‑1544691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑