Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 5,337,301 G→A G235R (GGG→AGG)  PP_4696 → nitrogen regulation protein NR(I)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,337,3010GA99.2% 854.0 / NA 242G235R (GGG→AGG) PP_4696nitrogen regulation protein NR(I)
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base A (118/121);  minor base C (2/0);  total (121/121)
Fisher's exact test for biased strand distribution p-value = 2.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 244 total aligned reads displayed.

AAAGAGCTGGTGGCCCGCGCCCTGCACAACCTGTCTCGCCGGGCCAAGGCACCGATGATCTCGGTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCGGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAG  >  NC_002947/5337154‑5337447
                                                                                                                                                   |                                                                                                                                                  
 aaGAGCTGGTGGCCCGCGCCCTGCACAACCTGTCTCGCCGGGCCAAGGCACCGATGATCTCGGTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGgc                                                                                                                                                 <  1:3221245/150‑1 (MQ=255)
 aaGAGCTGGTGGCCCGCGCCCTGCACAACCTGTCTCGCCGGGCCAAGGCACCGATGATCTCGGTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGgc                                                                                                                                                 >  2:2769815/1‑150 (MQ=255)
      cTGGTGGCCCGCGCCCTGCACAACCTGTCTCGCCGGGCCAAGGCACCGATGATCTCGGTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGcc                                                                                                                                            <  1:4984436/150‑1 (MQ=255)
      cTGGTGGCCCGCGCCCTGCACAACCTGTCTCGCCGGGCCAAGGCACCGATGATCTCGGTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGcc                                                                                                                                            >  2:4168057/1‑150 (MQ=255)
      cTGGTGGCCCGCGCCCTGCACAACCTGTCTCGCCGGGCCAAGGCACCGATGATCTCGGTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGcc                                                                                                                                            >  2:3102155/1‑150 (MQ=255)
            gCCCGCGCCCTGCACAACCTGTCTCGCCGGGCCAAGGCACCGATGATCTCGGTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTtg                                                                                                                                      >  2:4886103/1‑150 (MQ=255)
            gCCCGCGCCCTGCACAACCTGTCTCGCCGGGCCAAGGCACCGATGATCTCGGTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTtg                                                                                                                                      >  1:2670231/1‑150 (MQ=255)
              ccGCGCCCTGCACAACCTGTCTCGCCGGGCCAAGGCACCGATGATCTCGGTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTtggt                                                                                                                                    >  2:4843879/1‑150 (MQ=255)
              ccGCGCCCTGCACAACCTGTCTCGCCGGGCCAAGGCACCGATGATCTCGGTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCACGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTtggt                                                                                                                                    >  2:4286485/1‑150 (MQ=255)
                           aaCCTGTCTCGCCGGGCCAAGGCACCGATGATCTCGGTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGAt                                                                                                                       >  2:3604490/1‑150 (MQ=255)
                             ccTGTCTCGCCGGGCCAAGGCACCGATGATCTCGGTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATgg                                                                                                                     <  1:3013199/150‑1 (MQ=255)
                               tGTCTCGCCGGGCCAAGGCACCGATGATCTCGGTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCg                                                                                                                   <  1:3385456/150‑1 (MQ=255)
                               tGTCTCGCCGGGCCAAGGCACCGATGATCTCGGTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCg                                                                                                                   <  2:4364615/150‑1 (MQ=255)
                                   tcGCCGGGCCAAGGCACCGATGATCTCGGTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCGGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGtcc                                                                                                               >  2:2605330/1‑148 (MQ=255)
                                   tcGCCGGGCCAAGGCACCGATGATCTCGGTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTAc                                                                                                               >  1:4356846/1‑150 (MQ=255)
                                    cGCCGGGCCAAGGCACCGATGATCTCGGTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCGTGGTGGAGGCCGCCGATGGCGGTACg                                                                                                              >  2:1532257/1‑150 (MQ=255)
                                       cGGGCCAAGGCACCGATGATCTCGGTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTg                                                                                                           <  1:1952047/150‑1 (MQ=255)
                                        gggCCAAGGCACCGATGATCTCGGTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGt                                                                                                          >  1:2044863/1‑150 (MQ=255)
                                           ccAAGGCACCGATGATCTCGGTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTcc                                                                                                       >  2:3175804/1‑150 (MQ=255)
                                            cAAGGCACCGATGATCTCGGTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCt                                                                                                      >  2:1175382/1‑150 (MQ=255)
                                             aaGGCACCGATGATCTCGGTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTc                                                                                                     <  2:4688354/150‑1 (MQ=255)
                                             aaGGCACCGATGATCTCGGTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTGGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTc                                                                                                     >  2:4587802/1‑150 (MQ=255)
                                              aGGCACCGATGATCTCGGTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGtagcctt                                                                                                    <  2:570139/150‑7 (MQ=255)
                                               ggCACCGATGATCTCGGTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGa                                                                                                   <  1:2010861/150‑1 (MQ=255)
                                                gCACCGATGATCTCGGTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGGTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGAt                                                                                                  <  2:1129934/150‑1 (MQ=255)
                                                  aCCGATGATCTCGGTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTCCGCTGTTCCTCGAGGa                                                                                                >  2:3258909/1‑150 (MQ=255)
                                                    cGATGATCTCGGTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCGACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCCGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGaaa                                                                                              >  2:1058921/1‑150 (MQ=255)
                                                    cGATGATCTCGGTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGaaa                                                                                              >  2:258853/1‑150 (MQ=255)
                                                    cGATGATCTCGGTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGaaa                                                                                              >  1:4800672/1‑150 (MQ=255)
                                                      atgatCTCGGTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAAtt                                                                                            >  2:242057/1‑150 (MQ=255)
                                                       tgatCTCGGTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTg                                                                                           <  2:4119683/150‑1 (MQ=255)
                                                        gatCTCGGTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTgg                                                                                          <  2:1750054/150‑1 (MQ=255)
                                                         atCTCGGTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGc                                                                                         <  1:47780/150‑1 (MQ=255)
                                                          tctcGGTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCg                                                                                        >  1:2794331/1‑150 (MQ=255)
                                                          tctcGGTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCg                                                                                        <  2:1136835/150‑1 (MQ=255)
                                                           ctcGGTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGa                                                                                       >  1:1491594/1‑150 (MQ=255)
                                                            tcGGTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGaa                                                                                      <  1:2376049/150‑1 (MQ=255)
                                                              ggTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACt                                                                                    <  1:988702/150‑1 (MQ=255)
                                                              ggTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACt                                                                                    >  2:2222697/1‑150 (MQ=255)
                                                              ggTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACt                                                                                    >  2:1580525/1‑150 (MQ=255)
                                                               gTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGAGGAAATTGGCGAACTg                                                                                   >  1:4101545/1‑150 (MQ=255)
                                                                tGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGc                                                                                  >  2:2937008/1‑150 (MQ=255)
                                                                    cTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCt                                                                              >  2:4034647/1‑150 (MQ=255)
                                                                     tGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAATGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTc                                                                             >  2:2341359/1‑150 (MQ=255)
                                                                     tGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTc                                                                             <  1:3108698/150‑1 (MQ=255)
                                                                       cgcgGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGa                                                                           <  2:1905771/150‑1 (MQ=255)
                                                                        gcgGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGaa                                                                          <  2:497427/150‑1 (MQ=255)
                                                                          ggCCCTCCCCGAAACGCTGATCGAATCCGACCGGTTCGGCCACGACAAAGGCGCGTGCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGCGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAACTCGGCGAACTGCCGCTCGAAGc                                                                        <  2:2089089/150‑1 (MQ=255)
                                                                            ccATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGccc                                                                      <  1:4617717/150‑1 (MQ=255)
                                                                             cATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCa                                                                     <  1:924442/150‑1 (MQ=255)
                                                                             cATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCa                                                                     <  1:2937008/150‑1 (MQ=255)
                                                                              aTCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAg                                                                    >  1:3593348/1‑150 (MQ=255)
                                                                               tCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAgg                                                                   <  1:4006843/150‑1 (MQ=255)
                                                                                      aaCGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCct                                                            <  1:4580600/150‑1 (MQ=255)
                                                                                        cGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCctgc                                                          <  2:2232485/150‑1 (MQ=255)
                                                                                        cGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCctgc                                                          <  2:3030749/150‑1 (MQ=255)
                                                                                         gCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCctgct                                                         >  2:1822782/1‑150 (MQ=255)
                                                                                          cTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCctgctg                                                        <  2:4704679/150‑1 (MQ=255)
                                                                                           tGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCtgctgc                                                       <  1:1168209/150‑1 (MQ=255)
                                                                                           tGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCtgctgc                                                       <  1:2619294/150‑1 (MQ=255)
                                                                                           tGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCtgctgc                                                       >  1:3494607/1‑150 (MQ=255)
                                                                                             aTCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCgt                                                     <  1:1633442/150‑1 (MQ=255)
                                                                                             aTCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCgt                                                     <  1:2831632/150‑1 (MQ=255)
                                                                                              tCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCgtg                                                    >  2:1963884/1‑150 (MQ=255)
                                                                                               cGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCgtgt                                                   <  1:505161/150‑1 (MQ=255)
                                                                                                gAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGtgtg                                                  >  1:569518/1‑150 (MQ=255)
                                                                                                gAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGtgtg                                                  >  2:2157271/1‑150 (MQ=255)
                                                                                                   tCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGtgctg                                               >  1:2721026/1‑150 (MQ=255)
                                                                                                     cGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCa                                             <  1:3294532/150‑1 (MQ=255)
                                                                                                     cGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCa                                             <  1:3630835/150‑1 (MQ=255)
                                                                                                      gAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAg                                            >  2:725370/1‑150 (MQ=255)
                                                                                                      gAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAg                                            >  2:3511454/1‑150 (MQ=255)
                                                                                                           gTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAgg                                       <  2:61083/150‑1 (MQ=255)
                                                                                                              cGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCga                                    <  1:4152939/150‑1 (MQ=255)
                                                                                                                gCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTGCGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCgaga                                  >  2:103441/1‑150 (MQ=255)
                                                                                                                   aCGAAAAAGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATAc                               >  1:290062/1‑150 (MQ=255)
                                                                                                                      aaaaaGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGcc                            <  1:3837207/150‑1 (MQ=255)
                                                                                                                        aaaGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCgg                          <  1:1098373/150‑1 (MQ=255)
                                                                                                                        aaaGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCgg                          <  1:2583553/150‑1 (MQ=255)
                                                                                                                        aaaGGCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCgg                          <  2:3983943/150‑1 (MQ=255)
                                                                                                                           ggCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTa                       >  1:1207183/1‑150 (MQ=255)
                                                                                                                           ggCGCGTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTa                       >  2:3691184/1‑150 (MQ=255)
                                                                                                                            gcacgTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAg                      <  1:4286485/147‑1 (MQ=255)
                                                                                                                                gTTCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTc                  <  1:4955743/150‑1 (MQ=255)
                                                                                                                                  tCACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCgg                <  1:4843889/150‑1 (MQ=255)
                                                                                                                                   cACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGt               <  2:3881796/150‑1 (MQ=255)
                                                                                                                                   cACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGt               <  2:4214014/150‑1 (MQ=255)
                                                                                                                                   cACCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGt               <  2:3323833/150‑1 (MQ=255)
                                                                                                                                    aCCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTg              <  1:72855/150‑1 (MQ=255)
                                                                                                                                    aCCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTg              <  1:4886103/150‑1 (MQ=255)
                                                                                                                                    aCCGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTg              >  1:1799138/1‑150 (MQ=255)
                                                                                                                                    aCCGGCGCCAGCGCCAGGCGCGCCGGCTGGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCGCGACGCCCAGGCTCGCCTGCTGCGGGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTg              >  2:5093669/1‑150 (MQ=255)
                                                                                                                                      cggcgccggcgcCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCa            <  2:3828528/150‑1 (MQ=255)
                                                                                                                                      cGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCa            >  2:1955347/1‑150 (MQ=255)
                                                                                                                                      cGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCa            >  2:4896019/1‑150 (MQ=255)
                                                                                                                                      cGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCa            <  1:1800966/150‑1 (MQ=255)
                                                                                                                                      cGGCGCCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCa            >  2:4112449/1‑150 (MQ=255)
                                                                                                                                          gcCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCg        <  1:2789238/150‑1 (MQ=255)
                                                                                                                                          gcCAGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCg        <  1:4168057/150‑1 (MQ=255)
                                                                                                                                             aGCGCCAGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCaa     >  2:873399/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AAAGAGCTGGTGGCCCGCGCCCTGCACAACCTGTCTCGCCGGGCCAAGGCACCGATGATCTCGGTGAACTGCGCGGCCATCCCCGAAACGCTGATCGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGTTCACCGGCGCCAGCGCCGGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAG  >  NC_002947/5337154‑5337447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑