Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,030,462 T→G F173V (TTC→GTC)  PP_0887 → sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,030,4620TG97.7% 141.7 / NA 43F173V (TTC→GTC) PP_0887sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (24/18);  total (24/19)
Fisher's exact test for biased strand distribution p-value = 4.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTGCTGTATTACTCCGGCGGCTCGACCAACCCGTTCGTGTCGTATTACCTGGTGCCGCTGGCGATCGCTGCGGTGACCTTGCCGTGGCTGTATTCGCTGATCCTGTCGGGCATTGCGCTGACGGCCTACAGCTTGCTGCTGGTGCAGTTCTACCCGCTCGAAGGGCTGCCGATGGCGCGGGACAAGATGCAGGTCTACGGCATGTGGCTGAGCATTGCCCTGGCGGCGGCTGTGATCACCTTTTTTGCCGCGCGCATGGCCGAAGAGCTGCGCCGCCAGGAGCAGCTGCGTTCA  >  NC_002947/1030314‑1030608
                                                                                                                                                    |                                                                                                                                                  
cTTGCTGTATTACTCCGGCGGCTCGACCAACCCGTTCGTGTCGTATTACCTGGTGCCGCTGGCGATCGCTGCGGTGACCTTGCCGTGGCTGTATTCGCTGATCCTGTCGGGCATTGCGCTGACGGCCTACAGCTTGCTGCTGGTGCAGtt                                                                                                                                                   >  2:150448/1‑150 (MQ=255)
            cTCCGGCGGCTCGACCAACCCGTTCGTGTCGTATTACCTGCTGCCGCTGGCGATCGCTGCGGTGACCTTGCCGTGGCTGTATTCGCTGATCCTGTCGGGCATTGCGCTGACGGCCTACAGCTTGCTGCTGGTGCAGGTCTACCCGCTCGa                                                                                                                                       >  1:1142858/1‑150 (MQ=255)
             tCCGGCGGCTCGACCAACCCGTTCGTGTCGTATTACCTGGTGCCGCTGGCGATCGCTGCGGTGACCTTGCCGTGGCTGTATTCGCTGATCCTGTCGGGCATTGCGCTGACGGCCTACAGCTTGCTGCTGGTGCAGGTCTACCCGCTCGaa                                                                                                                                      <  2:1142858/150‑1 (MQ=255)
               cggcggCTCGACCAACCCGTTCGTGTCGTATTACCTGGTGCCGCTGGCGATCGCTGCGGTGACCTTGCCGTGGCTGTATTCGCTGATCCTGTCGGGCATTGCGCTGACGGCCTACAGCTTGCTGCTGGTGCAGGTCTAcc                                                                                                                                              >  2:1186728/1‑140 (MQ=255)
               cggcggCTCGACCAACCCGTTCGTGTCGTATTACCTGGTGCCGCTGGCGATCGCTGCGGTGACCTTGCCGTGGCTGTATTCGCTGATCCTGTCGGGCATTGCGCTGACGGCCTACAGCTTGCTGCTGGTGCAGGTCTAcc                                                                                                                                              <  1:1186728/140‑1 (MQ=255)
                     cTCGACCAACCCGTTCGTGTCGTATTACCTGGTGCCGCTGGCGATCGCTGCGGTGACCTTGCCGTGGCTGTATTCGCTGATCCTGTCGGGCATTGCGCTGACGGCCTACAGCTTGCTGCTGGTGCAGGTCTACCCGCTCGAAGGGCTGcc                                                                                                                              <  2:818380/150‑1 (MQ=255)
                           cAACCCGTTCGTGTCGTATTACCTGGTGCCGCTGGCGATCGCTGCGGTGACCTTGCCGTGGCTGTATTCGCTGATCCTGTCGGGCATTGCGCTGACGGCCTACAGCTTGCTGCTGGTGCAGGTc                                                                                                                                                  <  1:247463/124‑1 (MQ=255)
                           cAACCCGTTCGTGTCGTATTACCTGGTGCCGCTGGCGATCGCTGCGGTGACCTTGCCGTGGCTGTATTCGCTGATCCTGTCGGGCATTGCGCTGACGGCCTACAGCTTGCTGCTGGTGCAGGTc                                                                                                                                                  >  2:247463/1‑124 (MQ=255)
                               ccGTTCGTGTCGTATTACCTGGTGCCGCTGGCGATCGCTGCGGTGACCTTGCCGTGGCTGTATTCGCTGATCCTGTCGCGCATTGCGCTGACGGCCTCTAACTTTCTACTCATGCAGTTTTACCCGCTCGAAGGGCTGCCGATGGCGCgg                                                                                                                    <  1:150448/150‑1 (MQ=255)
                                      tgtCGTATTACCTGGTGCCGCTGGCGATCGCTGCGGTGACCTTGCCGTGGCTGTATTCGCTGATCCTGTCGGGCATTGCGCTGACGGCCTACAGCTTGCTGCTGGTGCAGGTCTACCCGCTCGAAGGGCTGCCGATGGCGCGGGACAAGa                                                                                                             >  2:938791/1‑150 (MQ=255)
                                      tgtCGTATTACCTGGTGCCGCTGGCGATCGCTGCGGTGACCTTGCCGTGGCTGTATTCGCTGATCCTGTCGGGCATTGCGCTGACGGCCTACAGCTTGCTGCTGGTGCAGGTCTACCCGCTCGAAGGGCTGCCGATGGCGCGGGACAAGa                                                                                                             >  2:827091/1‑150 (MQ=255)
                                         cGTATTACCTGGTGCCGCTGGCGATCGCTGCGGTGACCTTGCCGTGGCTGTATTCGCTGATCCTGTCGGGCATTGCGCTGACGGCCTACAGCTTGCTGCTGGTGCAGGTCTACCCGCTCGAAGGGCTGCCGATGGCGCGGGACAAGATGc                                                                                                          <  2:783008/150‑1 (MQ=255)
                                           tATTACCTGGTGCCGCTGGCGATCGCTGCGGTGACCTTGCCGTGGCTGTATTCGCTGATCCTGTCGGGCATTGCGCTGACGGCCTACAGCTTGCTGCTGGTGCAGGTCTACCCGCTCGAAGGGCTGCCGATGGCGCGGGACAAGATGCAg                                                                                                        >  1:275848/1‑150 (MQ=255)
                                           tATTACCTGGTGCCGCTGGCGATCGCTGCGGTGACCTTGCCGTGGCTGTATTCGCTGATCCTGTCGGGCATTGCGCTGACGGCCTACAGCTTGCTGCTGGTGCAGGTCTACCCGCTCGAAGGGCTGCCGATGGCGCGGGACAAGATGCAg                                                                                                        <  2:406608/150‑1 (MQ=255)
                                           tATTACCTGGTGCCGCTGGCGATCGCTGCGGTGACCTTGCCGTGGCTGTATTCGCTGATCCTGTCGGGCATTGCGCTGACGGCCTACAGCTTGCTGCTGGTGCAGGTCTACCCGCTCGAAGGGCTGCCGATGGCGCGGGACAAGATGCAg                                                                                                        >  1:451155/1‑150 (MQ=255)
                                           tATTACCTGGTGCCGCTGGCGATCGCTGCGGTGACCTTGCCGTGGCTGTATTCGCTGATCCTGTCGGGCATTGCGCTGACGGCCTACAGCTTGCTGCTGGTGCAGGTCTACCCGCTCGAAGGGCTGCCGATGGCGCGGGACAAGATGCAg                                                                                                        <  2:128863/150‑1 (MQ=255)
                                             ttACCTGGTGCCGCTGGCGATCGCTGCGGTGACCTTGCCGTGGCTGTATTCGCTGATCCTGTCGGGCATTGCGCTGACGGCCTACAGCTTGCTGCTGGTGCAGGTCTACCCGCTCGAAGGGCTGCCGATGGCGCGGGACAAGATGCAGGt                                                                                                      >  2:295653/1‑150 (MQ=255)
                                                 cTGGTGCCGCTGGCGATCGCTGCGGTGACCTTGCCGTGGCTGTATTCGCTGATCCTGTCGGGCATTGCGCTGACGGCCTACAGCTTGCTGCTGGTGCAGGTCTACCCGCTCGAAGGGCTGCCGATGGCGCGGGACAAGATGCAGGTCTAc                                                                                                  >  1:634220/1‑150 (MQ=255)
                                                    gTGCCGCTGGCGATCGCTGCGGTGACCTTGCCGTGGCTGTATTCGCTGATCCTGTCGGGCATTGCGCTGACGGCCTACAGCTTGCTGCTGGTGCAGGTCTACCCGCTCGAAGGGCTGCCGATGGCGCGGGACAAGATGCAGGTCTACGGc                                                                                               >  2:832131/1‑150 (MQ=255)
                                                      gCCGCTGGCGATCGCTGCGGTGACCTTGCCGTGGCTGTATTCGCTGATCCTGTCGGGCATTGCGCTGACGGCCTACAGTTTGCTGCTGGTGCAGGTCTACCCGCTCGAAGGTCTGCCGATGGCGCGGGACAAGATGCAGGTCTACGGCAt                                                                                             <  1:519102/150‑1 (MQ=255)
                                                      gCCGCTGGCGATCGCTGCGGTGACCTTGCCGTGGCTGTATTCGCTGATCCTGTCGGGCATTGCGCTGACGGCCTACAGCTTGCTGCTGGTGCAGGTCTACCCGCTCGAAGGGCTGCCGATGGCGCGGGACAAGATGCAGGTCTACGGCAt                                                                                             <  1:295653/150‑1 (MQ=255)
                                                       ccGCTGGCGATCGCTGCGGTGACCTTGCCGTGGCTGTATTCGCTGATCCTGTCGGGCATTGCGCTGACGGCCTACAGCTTGCTGCTGGTGCAGGTCTACCCGCTCGAAGGGCTGCCGATGGCGCGGGACAAGATg                                                                                                           >  2:712175/1‑135 (MQ=255)
                                                       ccGCTGGCGATCGCTGCGGTGACCTTGCCGTGGCTGTATTCGCTGATCCTGTCGGGCATTGCGCTGACGGCCTACAGCTTGCTGCTGGTGCAGGTCTACCCGCTCGAAGGGCTGCCGATGGCGCGGGACAAGATg                                                                                                           <  1:712175/135‑1 (MQ=255)
                                                                           gACCTTGCCGTGGCTGTATTCGCTGATCCTGTCGGGCATTGCGCTGACGGCCTACAGCTTGCTGCTGGTGCAGGTCTACCCGCTCGAAGGGCTGCCGATGGCGCGGGACAAGATGCAGGTCTACGGCATGTGGCTGAGCATTGCCCTggc                                                                        <  2:451155/150‑1 (MQ=255)
                                                                             ccTTGCCGTGGCTGTATTCGCTGATCCTGTCGGGCATTGCGCTGACGGCCTACAGCTTGCTGCTGGTGCAGGTCTACCCGCTCGAAGGGCTGCCGATGGCGCGGGACAAGATGCAGGTCTACGGCATGTGGCTGAGCATTGCCCTggcgg                                                                      <  1:832131/150‑1 (MQ=255)
                                                                               ttGCCGTGGCTGTATTCGCTGATCCTGTCGGGCATTGCGCTGACGGCCTACAGCTTGCTGCTGGTGCAGGTCTACCCGCTCGAAGGGCTGCCGATGGCGCGGGACAAGATGCAg                                                                                                        >  1:1027276/1‑114 (MQ=255)
                                                                               ttGCCGTGGCTGTATTCGCTGATCCTGTCGGGCATTGCGCTGACGGCCTACAGCTTGCTGCTGGTGCAGGTCTACCCGCTCGAAGGGCTGCCGATGGCGCGGGACAAGATGCAg                                                                                                        <  2:1027276/114‑1 (MQ=255)
                                                                                  ccGTGGCTGTATTCGCTGATCCTGTCGGGCATTGCGCTGACGGCCTACAGCTTGCTGCTGGTGCAGGTCTACCCGCTCGAAGGGCTGCCGATGGCGCGGGACAAGATGCAGGTCTACGGCATGTGGCTGAGCATTGCCCTGGCGGCGGCt                                                                 >  2:661050/1‑150 (MQ=255)
                                                                                       gCTGTATTCGCTGATCCTGTCGGGCATTGCGCTGACGGCCTACAGCTTGCTGCTGGTGCAGGTCTACCCGCTCGAAGGGCTGCCGATGGCGCGGGACAAGATGCAGGTCTACGGCATGTGGCTGAGCATTGCCCTGGCGGCGGCTGTGAt                                                            >  2:1158651/1‑150 (MQ=255)
                                                                                        cTGTATTCGCTGATCCTGTCGGGCATTGCGCTGACGGCCTACAGCTTGCTGCTGGTGCAGGTCTACCCGCTCGAAGGGCTGCCGATGGCGCGGGACAAGATGCAGGTCTACGGCATGTGGCTGAGCATTGCCCTGGCGGCGGCTGTGATc                                                           >  2:786904/1‑150 (MQ=255)
                                                                                          gTATTCGCTGATCCTGTCGGGCATTGCGCTGACGGCCTACAGCTTGCTGCTGGTGCAGGTCTACCCGCTCGAAGGGCTGCCGATGGCGCGGGACAAGATGCAGGTCTACGGCATGTGGCTGAGCATTGCCCTGGCGGCGGCTGTGATCAc                                                         <  1:827091/150‑1 (MQ=255)
                                                                                                gCTGATCCTGTCGGGCATTGCGCTGACGGCCTACAGCTTGCTGCTGGTGCAGGTCTACCCGCTCGAAg                                                                                                                                     >  1:758053/1‑68 (MQ=255)
                                                                                                gCTGATCCTGTCGGGCATTGCGCTGACGGCCTACAGCTTGCTGCTGGTGCAGGTCTACCCGCTCGAAg                                                                                                                                     <  2:758053/68‑1 (MQ=255)
                                                                                                  tGATCCTGTCGGGCATTGCGCTGACGGCCTACAGCTTGCTGCTGGTGCAGGTCTACCCGCTCGAAGGGCTGCCGATGGCGCGGGACAAGATGCAGGTCTACGGCATGTGGCTGAGCATTGCCCTGGCGGCGGCTGTGATCACCTTTTTTg                                                 >  2:677284/1‑150 (MQ=255)
                                                                                                        tGTCGGGCATTGCGCTGACGGCCTACAGCTTGCTGCTGGTGCAGGTCTACCCGCTCGAAGGGCTGCCGATGGCGCGGGACAAGATGCAGGTCTACGGCATGTGGCTGAGCATTGCCCTGGCGGCGGCTGTGATCACCTTTTTTGCcgcgc                                           >  2:1121319/1‑150 (MQ=255)
                                                                                                          tCGGGCATTGCGCTGACGGCCTACAGCTTGCTGCTGGTGCAGGTCTACCCGCTCGAAGGGCTGCCGATGGCGCGGGACAAGATGCAGGTCTACGGCATGTGGCTGAGCATTGCCCTGGCGGCGGCTGTGATCACCTTTTTTGCcgcgggg                                         >  1:167943/1‑147 (MQ=255)
                                                                                                            gggCATTGCGCTGACGGCCTACAGCTTGCTGCTGGTGCAGGTCTACCCGCTCGAAGGGCTGCCGATGGCGCGGGACAAGATGCAGGTCTACGGCATGTGGCTGAGCATTGCCCTGGCGGCGGCTGTGATCACCTTTTTTGCCGCGCGCAt                                       >  2:617476/1‑150 (MQ=255)
                                                                                                                                 cAGCTTGCTGCTGGTGCAGGTCTACCCGCTCGAAGGGCTGCCGATGGCGCGGGACAAGATGCAGGTCTACGGCATGTGGCTGAGCATTGCCCTGGCGGCGGCTGTGATCACCTTTTTTGCCGCGCGCATGGCCGAAGAGCTGCGCCGCCa                  <  2:634220/150‑1 (MQ=255)
                                                                                                                                  aGCTTGCTGCTGGTGCAGGTCTACCCGCTCGAAGGGCTGCCGATGGCGCGGGACAAGATGCAGGTCTACGGCATGTGGCTGAGCATTGCCCTGGCGGCGGCTGTGATCACCTTTTTTGCCGCGCGCATGGCCGAAGAGCTGCGCCGCCAg                 >  1:680390/1‑150 (MQ=255)
                                                                                                                                     ttGCTGCTGGTGCAGGTCTACCCGCTCGAAGGGCTGCCGATGGCGCGGGACAAGATGCAGGTCTACGGCATGTGGCTGAGCATTGCCCTGGCGGCGGCTGTGATCACCTTTTTTGCCGCGCGCATGGCCGAAGAGCTGCGCCGCCAGGag              >  2:184514/1‑150 (MQ=255)
                                                                                                                                       gctgctGGTGCAGGTCTACCCGCTCGAAGGGCTGCCGATGGCGCGGGACAAGATGCAGCTCTACGGCATGTGGCTTAGCATTGCCCTGGCGGCGGCTGTGATCCCCTTTTTTGGCGCGCGCATGTCCGAAGACGTGCTCCGCTAGGagcc            >  2:259272/1‑149 (MQ=255)
                                                                                                                                         tgctgGTGCAGGTCTACCCGCTCGAAGGGCTGCCGATGGCGCGGGACAAGATGCAGGTCTACGGCATGTGGCTGAGCATTGCCCTGGCGGCGGCTGTGATCACCTTTTTTGCCGCGCGCATGGCCGAAGAGCTGCGCCGCCAGGagcagc          <  1:786904/150‑1 (MQ=255)
                                                                                                                                         tgctgGTGCAGGTCTACCCGCTCGAAGGGCTGCCGATGGCGCGGGACAAGATGCAGGTCTACGGCAGGTGGCTGAGCATTGCCCTGGCGGCGGCTGTGATCACCGTTTTTGCCGCGCGCATGGCCGAAGAGCTGCGCCGCCAGGagcagc          >  2:636436/1‑150 (MQ=255)
                                                                                                                                                 cAGGTCTACCCGCTCGAAGGGCTGCCGATGGCGCGGGACAAGATGCAGGTCTACGGCATGTGGCTGAGCATTGCCCTGGCGGCGGCTGTGATCACCTTTTTTGCCGCGCGCATGGCCGAAGAGCTGCGCCGCCAGGAGCAGCTGCGTTCa  <  1:677284/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CTTGCTGTATTACTCCGGCGGCTCGACCAACCCGTTCGTGTCGTATTACCTGGTGCCGCTGGCGATCGCTGCGGTGACCTTGCCGTGGCTGTATTCGCTGATCCTGTCGGGCATTGCGCTGACGGCCTACAGCTTGCTGCTGGTGCAGTTCTACCCGCTCGAAGGGCTGCCGATGGCGCGGGACAAGATGCAGGTCTACGGCATGTGGCTGAGCATTGCCCTGGCGGCGGCTGTGATCACCTTTTTTGCCGCGCGCATGGCCGAAGAGCTGCGCCGCCAGGAGCAGCTGCGTTCA  >  NC_002947/1030314‑1030608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑