Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,843,613	T→C	N408S (AAC→AGC)	gacS ←	sensor protein GacS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,843,613	0	T	C	100.0%	165.1 / NA	49	N408S (AAC→AGC)	gacS	sensor protein GacS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/27);  total (22/27)

TGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAATTCCAGCTGCTTGGCGTGGGCGGCCGGAGCGAGGATGGTCAGGGTGTCCTGGATCAGGTCCCGCAGGTT  >  NC_002947/1843468‑1843758                                                                                                                                                  |                                                                                                                                                  tgtgCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGCTGGt                                                                                                                                               >  1:542003/1‑150 (MQ=255)          aTCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGCTGGTGAGGATCTg                                                                                                                                      >  2:302469/1‑150 (MQ=255)                      cAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCaa                                                                                                                          <  2:542003/150‑1 (MQ=255)                       aGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAAc                                                                                                                         <  1:189478/150‑1 (MQ=255)                          ccGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACggg                                                                                                                      <  2:328574/150‑1 (MQ=255)                            gATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTc                                                                                                                    <  2:18562/150‑1 (MQ=255)                                      tCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGc                                                                                                          <  2:1106804/150‑1 (MQ=255)                                              gCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCg                                                                                                         >  1:443799/1‑143 (MQ=255)                                              gCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCg                                                                                                         <  2:443799/143‑1 (MQ=255)                                              gCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATCGCGATAACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGCTTGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAgg                                                                                                  >  1:518472/1‑150 (MQ=255)                                               cTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGgt                                                                                                 <  1:920267/150‑1 (MQ=255)                                                       cAACTGCGCGCAGTCTTCGTGATCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGGGAACTTGAGGGCGTTGCTGACCAGGCTGGTGAGGATCGGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGCTGTGTcgcg                                                                                         <  2:339111/150‑1 (MQ=255)                                                        aaCTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCgg                                                                                        >  2:1000372/1‑150 (MQ=255)                                                        aaCTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCgg                                                                                        >  1:252917/1‑150 (MQ=255)                                                               cgcAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCa                                                                                 <  1:708240/150‑1 (MQ=255)                                                                 cAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAgg                                                                               <  1:441678/150‑1 (MQ=255)                                                                          tGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAAt                                                                      <  1:609076/150‑1 (MQ=255)                                                                            cTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAATTc                                                                    <  1:256294/150‑1 (MQ=255)                                                                             tCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAgg                                                                                                  <  2:1008503/119‑1 (MQ=255)                                                                             tCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAgg                                                                                                  >  1:1008503/1‑119 (MQ=255)                                                                             tCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAATTcc                                                                   >  1:90662/1‑150 (MQ=255)                                                                                             cgcgcACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAATTCCAGCTGCTTGGCGTggg                                                   <  2:208697/150‑1 (MQ=255)                                                                                                 cACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAATTCCAGCTGCTTGGCGTGggcggc                                               <  2:688434/150‑1 (MQ=255)                                                                                                  acgacgATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCa                                                                                 <  1:577415/115‑1 (MQ=255)                                                                                                  acgacgATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCa                                                                                 >  2:577415/1‑115 (MQ=255)                                                                                                    gacgaTGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAATTc                                                                    >  1:699844/1‑126 (MQ=255)                                                                                                    gacgaTGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAATTc                                                                    <  2:699844/126‑1 (MQ=255)                                                                                                     acgaTGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAATTCCAGCTGCTTGGCGTGGGCGGCCGGa                                           >  2:39189/1‑150 (MQ=255)                                                                                                            tGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAATTCCAGCTGCTTGGCGTGGGCGGCCGGAGCGAGGa                                    >  1:1137040/1‑150 (MQ=255)                                                                                                              ccTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAATTCCAGCTGCTTGGCGTGGGCGGCCGGAGCGAGGa                                    >  2:997090/1‑148 (MQ=255)                                                                                                              ccTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAATTCCAGCTGCTTGGCGTGGGCGGCCGGAGCGAGGa                                    <  1:997090/148‑1 (MQ=255)                                                                                                               cTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAATTCCAGCTGCTTGGCGTg                                                     >  2:693826/1‑130 (MQ=255)                                                                                                               cTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAATTCCAGCTGCTTGGCGTg                                                     <  1:693826/130‑1 (MQ=255)                                                                                                               cTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAATTCCAGCTGCTTGGCGTGGGCGGCCGGAGCGAGGATgg                                 <  2:1137040/150‑1 (MQ=255)                                                                                                                ttCGCGGGTGAACTTGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGAt                                                                                   >  1:888091/1‑99 (MQ=255)                                                                                                                ttCGCGGGTGAACTTGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGAt                                                                                   <  2:888091/99‑1 (MQ=255)                                                                                                                ttCGCGGGTGAACTTGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAATTCCAGCTGCTTGGCGTGGGCGGCCGGAGCGAGGATGGt                                >  1:223742/1‑150 (MQ=255)                                                                                                                    cgGGTGAACTTGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAATTCCAGCTGCTTGGCGTGGGCGGCCGGAGCGAGGATGGTCAgg                            <  2:252917/150‑1 (MQ=255)                                                                                                                       gTGAACTTGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAATTCCAgc                                                                >  1:382542/1‑111 (MQ=255)                                                                                                                       gTGAACTTGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAATTCCAgc                                                                <  2:382542/111‑1 (MQ=255)                                                                                                                       gTGAACTTGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAATTCCAGCTGCTTGGCGTGGGCGGCCGGAGCGAGGATGGTCAGGgtg                         >  1:206479/1‑150 (MQ=255)                                                                                                                        tGAACTTGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAATTCCAGCTGCTTGGCGTGGGCGGCCGGAGCGAGGATGGTCAGGgtgt                        >  2:938490/1‑150 (MQ=255)                                                                                                                             ttGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCa                                                                           >  2:828322/1‑94 (MQ=255)                                                                                                                             ttGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCa                                                                           <  1:828322/94‑1 (MQ=255)                                                                                                                             ttGATGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAATTCCAGCTGCTTGGCGTGGGCGGCCGGAGCGAGGATGGTCAGGGTGTCCTgg                   <  2:90662/150‑1 (MQ=255)                                                                                                                                 tGGCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAATTCCAGCTGCTTGGCGTGGGCGGCCGGAGCGAGGATGGTCAGGGTGTCCTGGATCa               <  1:1000372/150‑1 (MQ=255)                                                                                                                                  ggCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAATTCCAGCTGCTTGGCGTGGGCGGCCGGAGCGAGGATGGTCAGGGTGTCCTGGATCAg              <  2:757986/150‑1 (MQ=255)                                                                                                                                   gCGTTGCTGACCAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGCGTGTCGCGGTAGATCAGGCTCAGCAATTCCAGCTGCTTGGCGTGGGCGGCCGGAGCGAGGATGGTCAGGGTGTCCTGGATCAgg             >  1:575015/1‑150 (MQ=255)                                                                                                                                              cAGGCTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAACAGGTGTGTCGCGGTAGATCAGGCTCAGCAATTCCAGCTGCTTGGCGTGGGCGGCCGGAGCGAGGATGGTCAGGGTGTCCTGGATCAGGTCCCGCAGGtt  >  1:131523/1‑150 (MQ=255)                                                                                                                                                  |                                                                                                                                                  TGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAATTCCAGCTGCTTGGCGTGGGCGGCCGGAGCGAGGATGGTCAGGGTGTCCTGGATCAGGTCCCGCAGGTT  >  NC_002947/1843468‑1843758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑