| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 497,706 | Δ1 bp | 9.7% | coding (1575/2388 nt) | PP_0409 → | two‑component system sensor histidine kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 497,706 | 0 | C | . | 9.7% | 183.5 / 15.3 | 62 | coding (1575/2388 nt) | PP_0409 | two‑component system sensor histidine kinase |
| Reads supporting (aligned to +/- strand): ref base C (30/25); new base . (2/4); total (33/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01 | |||||||||||
TCCCCTATATCAGTGAGGGTAGCGAGGCTTTGGTGGGTTATGCACCCAGCGAGATCCAGCACCCGCAGATGGGCCTGCGCAACCTGGTGCACCCCGAGGACCGTGCTGATTATCACAGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCATCCTCACCCGGCAGGGCGAACAGCGCTGGGCCGATATCAAGGCCAGCGCCCGGCATCTGGGCAACGGCCAGGTAGTGTGGGACGGTGTAGTCTGGGACATCACACAGGGC > NC_002947/497564‑497844 | tCCCCTATATCAGTGAGGGTAGCGAGGCTTTGGTGGGTTATGCACCCAGCGAGATCCAGCACCCGCAGATGGGCCTGCGCAACCTGGTGCACCCCGAGGACCGTGCTGATTATCACAGGGTGCAGGACCTGGCCCCTGCCAG‑GACCAgtc < 1:568948/150‑3 (MQ=255) tatCAGTGAGGGTAGCGAGGCTTTGGTGGGTTATGCACCCAGCGAGATCCAGCACCCGCAGATGGGCCTGCGCAACCTGGTGCACCCCGAGGACCGTGCTGATTATCACAGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTc < 2:281720/150‑1 (MQ=255) tatCAGTGAGGGTAGCGAGGCTTTGGTGGGTTATGCACCCAGCGAGATCCAGCACCCGCAGATGGGCCTGCGCAACCTGGTGCACCCCGAGGACCGTGCTGATTATCACAGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTc > 2:136912/1‑150 (MQ=255) cAGTGAGGGTAGCGAGGCTTTGGTGGGTTATGCACCCAGCGAGATCCAGCACCCGCAGATGGGCCTGCGCAACCTGGTGCACCCCGAGGACCGTGCTGATTATCACAGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACCAGTC‑CTg < 1:914997/150‑1 (MQ=255) aGTGAGGGTAGCGAGGCTTTGGTGGGTTATGCACCCAGCGAGATCCAGCACCCGCAGATGGGCCTGCGCAACCTGGTGCACCCCGAGGACCGTGCTGATTATCACAGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTC‑CTgg < 2:1412694/150‑1 (MQ=255) aGTGAGGGTAGCGAGGCTTTGGTGGGTTATGCACCCAGCGAGATCCAGCACCCGCAGATGGGCCTGCGCAACCTGGTGCACCCCGAGGACCGTGCTGATTATCACAGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTC‑CTgg > 1:832720/1‑150 (MQ=255) gAGGGTAGCGAGGCTTTGGTGGGTTATGCACCCAGCGAGATCCAGCACCCGCAGATGGGCCTGCGCAACCTGGTGCACCCCGAGGACCGTGCTGATTATCACAGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTC‑CTGGCAg < 1:280465/150‑1 (MQ=255) gAGGGTAGCGAGGCTTTGGTGGGTTATGCACCCAGCGAGATCCAGCACCCGCAGATGGGCCTGCGCAACCTGGTGCACAACAAGACGGGCACTTAGTAGCACAGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTC‑CTGGCAg > 1:168879/1‑150 (MQ=255) tgccctgtgtgatgtcccagactacaccgtcccacactacctggccgttgcccagatgccgggcgctggccttgatatcggcccagcgctgttcgccctgccGGGTG‑AGGATGCGCCCCTGGCCAGCGACCAGGACTGGTC‑CTGGCAggg > 1:1141817‑M1/103‑150 (MQ=255) gggTAGCGAGGCTTTGGTGGGTTATGCACCCAGCGAGATCTAGCACCCGCAGATGGGCCTGCGCAACCTGGTGCACCCCGAGGACCGTGCTGATTATCACAAGGGGCAGGTCCGGGCCCTGGCCAGCGAGGAGGACTGGTA‑TTGGCAggg > 2:945087/1‑150 (MQ=255) ggTAGCGAGGCTTTGGTGGGTTATGCACCCAGCGAGATCCAGCACCCGCAGATGGGCCCGCGCAACCTGGTGCACCCCGAGGACCGTGCTGATTATCACAGGGTGCAGGACCTGGTCCCTGCCAG‑GACCAGTCCTGGTCGCTGGccaggg < 1:1182492/150‑6 (MQ=255) cctgtgtgatgtcccagactacaccgtcccacactacctggccgttgcccagatgccgggcgctggccttgatatcggcccagcgctgttcgccctgccGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTC‑CTGGCAGGGgc < 1:236776‑M1/51‑1 (MQ=255) cGAGGCTTTGGTGGGTTATGCACCCAGCGAGATCCAGCACCCGCAGATGGGCCTGCGCAACCTGGTGCACCCCGAGGACCGTGCTGATTATCACAGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCAt > 1:1254714/1‑150 (MQ=255) cGAGGCTTTGGTGGGTTATGCACCCAGCGAGATCCAGCACCCGCAGATGGGCCTGCGCAACCTGGTGCACCCCGAGGACCGTGCTGATTATCACAGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCAt < 1:262835/150‑1 (MQ=255) tgatgtcccagactacaccgtcccacactacctggccgttgcccagatgccgggcgctggccttgatatcggcccagcgctgttcgccctgccGGGTGCAGGACCTGGTCCTGGCCAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCATcc > 1:844115‑M1/94‑150 (MQ=255) aGGCTTTGGTGGGTTATGCACCCAGCGAGATCCAGCACCCGCAGATGGGCCTGCGCAACCTGGTGCACCCCGAGGACCGTGCTGATTATCACAGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCATcc > 2:602270/1‑150 (MQ=255) tttGGTGGGTTATGCACCCAGCGAGATCCAGCACCCGCAGATGGGCCTGCGCAACCTGGTGCACCCCGAGGACCGTGCTGATTATCACAGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCATCCTCAc > 1:1306653/1‑150 (MQ=255) gtggGTTATGCACCCAGCGAGATCCAGCACCCGCAGATGGGCCTGCGCAACCTGGTGCACCCCGAGGACCGTGCTGATTATCACAGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCATCCTCACCCgg > 1:1023683/1‑150 (MQ=255) ctacaccgtcccacactacctggccgttgcccagatgccgggcgctggccttgatatcggcccagcgctgttcgccctgccGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCATCCTCACCCGGCAgg > 2:236776‑M1/82‑150 (MQ=255) ttATGCACCCAGCGAGATCCAGCACCCGCAGATGGGCCTGCGCAACCTGGTGCACCCCGAGGACCGTGCTGATTATCACAGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCATCCTCACCCGGCAggg < 2:832720/150‑1 (MQ=255) ccAGCGAGATCCAGCACCCGCAGATGGGCCTGCGCAACCTGGTGCACCCCGAGGACCGTGCTGATTATCACAGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTC‑Ct > 1:467509/1‑114 (MQ=255) ccAGCGAGATCCAGCACCCGCAGATGGGCCTGCGCAACCTGGTGCACCCCGAGGACCGTGCTGATTATCACAGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTC‑Ct < 2:467509/114‑1 (MQ=255) gCGAGATCCAGCACCCGCAGATGGGCCTGCGCAACCTGGTGCACCCCGAGGACCGTGCTGATTATCACAGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCATCCTCACCCGGCAGGGCGAActccaga < 2:1163941/150‑7 (MQ=255) agaTCCAGCACCCGCAGATGGGCCTGCGCAACCTGGTGCACCCCGAGGACCGTGCTGATTATCACAGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCATCCTCACCCGGCAGGGCGAACAGCGCTggg > 2:1178698/1‑150 (MQ=255) tCCAGCACCCGCAGATGGGCCTGCGCAACCTGGTGCACCCCGAGGACCGTGCTGATTATCACAGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACt < 1:443540/99‑1 (MQ=255) tCCAGCACCCGCAGATGGGCCTGCGCAACCTGGTGCACCCCGAGGACCGTGCTGATTATCACAGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACt > 2:443540/1‑99 (MQ=255) tCCAGCACCCGCAGATGGGCCTGCGCAACCTGGTGCACCCCGAGGACCGTGCTGATTATCACAGGGTGCAGGACCTGGCCCCTGCCAG‑GACCAGTCCTGGTCGCTGGccagg > 2:706438/1‑108 (MQ=25) tCCAGCACCCGCAGATGGGCCTGCGCAACCTGGTGCACCCCGAGGACCGTGCTGATTATCACAGGGTGCAGGACCTGGCCCCTGCCAG‑GACCAGTCCTGGTCGCTGGccagg < 1:706438/112‑5 (MQ=25) ccAGCACCCGCAGATGGGCCTGCGCAACCTGGTGCACCCCGAGGACCGTGCTGATTATCACAGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCATCCTCACCCGGCAGGGCGAACAGCGCTGGGCCGa < 1:136912/150‑1 (MQ=255) cAGCACCCGCAGATGGGCCTGCGCAACCTGGTGCACCCCGAGGACCGTGCTGATTATCACAGGGTGCAGGACCTGGCCCTAGCCAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCATCCTCACCCGGCAGGGCGAACAGCGCTGGGCCGat > 1:25330/1‑150 (MQ=255) ccgttgcccagatgccgggcgctggccttgatatcggcccagcgctgttcgccctgccGGGTG‑AGGATGCGCCCCTGGCCAGCGACCAGGACTGGTC‑CTGGCAg < 2:1081016‑M1/46‑1 (MQ=255) ccgttgcccagatgccgggcgctggccttgatatcggcccagcgctgttcgccctgccGGGTG‑AGGATGCGCCCCTGGCCAGCGACCAGGACTGGTC‑CTGGCAg > 1:1081016‑M1/59‑104 (MQ=255) ccGCAGATGGGCCTGCGCAACCTGGTGTACCCCGAGGACCGTGCTGATTATCACAGGGTGCAGGACCTGGCCCTGGCCAG‑GACCAGTCCTGGTcgctg > 1:669608/1‑94 (MQ=255) ccGCAGATGGGCCTGCGCAACCTGGTGTACCCCGAGGACCGTGCTGATTATCACAGGGTGCAGGACCTGGCCCTGGCCAG‑GACCAGTCCTGGTcgctg < 2:669608/98‑5 (MQ=255) ccGCAGATGGGCCTGCGCAACCTGGTGCACCCCGAGGACCGTGCTGATTATCACAGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCATCCTCACCCGGCAGGGCGAACAGCGCTGGGCCGATATCAAg < 2:1254714/150‑1 (MQ=255) ccGCAGATGGGCCTGCGCAACCTGGTGCACCCCGAGGACCGTGCTGATTATCACAGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCATCCTCACCCGGCAGGGCGAACAGCGCTGGGCCGATATCAAg < 2:1392164/150‑1 (MQ=255) gCAGATGGGCCTGCGCAACCTGGTGCACCCCGAGGACCGTGCTGATTATCACAGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCATCCTCACCCGGCAGGGCGAACa > 1:1056554/1‑129 (MQ=255) gCAGATGGGCCTGCGCAACCTGGTGCACCCCGAGGACCGTGCTGATTATCACAGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCATCCTCACCCGGCAGGGCGAACa < 2:1056554/129‑1 (MQ=255) aGATGGGCCTGCGCAACCTGGTGCACCCCGAGGACCGTGCTGATTATCACAGGGTGCAGGCCCTGGCCAACGCCAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCATCCTCACCCGGCAGGGCGAACAGCGCTGGGCCGATATCAAGGCCa < 1:602270/150‑1 (MQ=255) gcAACCTGGTGCACCCCGAGGACCGTGCTGATTATCACAGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCATCCTCACCCGGCAGGGCGAACAGCGCTGGGCCGATATCAAGGCCAGCGCCCGGCATc > 2:522007/1‑150 (MQ=255) cTGGTGCACCCCGAGGACCGTGCTGATTATCACAGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCATCCTCACCCGGCAGGGCGAACAGCGCTGGGCCGATATCAAGGCCAGCGCCCGGCATCTGGGc > 1:90599/1‑150 (MQ=255) aCCCCGAGGACCGTGCTGATTATCACAGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCATCCTCACCCGGCAGGGCGAACAGCGCTGGGCCGATATCAAGGCCAGCGCCCGGCATCTGGGCAACGGcc > 2:897386/1‑150 (MQ=255) ccccGAGGACCGTGCTGATTATCACAGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCATCCTCACCCGGCAGGGCGAACAGCGCTGGGCCGATATCAAGGCCAGCGCCCGGCATCTGGGCAACGGCCa > 2:248584/1‑150 (MQ=255) ccccGAGGACCGTGCTGATTATCACAGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCATCCTCACCCGGCAGGGCGAACAGCGCTGGGCCGATATCAAGGCCAGCGCCCGGCATCTGGGCAACGGCCa > 2:1376161/1‑150 (MQ=255) cGTGCTGATTATCACAGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCATCCTCACCCGGCAGGGCGAACAGCGCTGGGCCGATATCAAGGCCAGCGCCCGGCATCTGGGCAACGGCCAGGTAGTGTgg > 1:1117582/1‑150 (MQ=255) tGCTGATTATCACAGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCATCCTCACCCGGCAGGGCGAACAGCGCTGGGCCGat < 1:1396067/103‑1 (MQ=255) tGCTGATTATCACAGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCATCCTCACCCGGCAGGGCGAACAGCGCTGGGCCGat > 2:1396067/1‑103 (MQ=255) tCACAGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCATCCTCACCCGGCAGGGCGAACAGCGCTGGGCCGATATCAAGGCCAGCGCCCGGCATCTg > 2:652481/1‑118 (MQ=255) tCACAGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCATCCTCACCCGGCAGGGCGAACAGCGCTGGGCCGATATCAAGGCCAGCGCCCGGCATCTg < 1:652481/118‑1 (MQ=255) tCACAGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCATCCTCACCCGGCAGGGCGAACAGCGCTGGGCCGATATCAAGGCCAGCGCCCGGCATCTGGGCAACGGCCAGGTAGTGTGGGACGGTGTAGt < 1:522007/150‑1 (MQ=255) caGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCATCCTCACCCGGCAGGGCGAACAGCGCTGGGCCGATATCAAGGCCAGCGCCCGGCATCTGGGCAACGGCCAGGTAGTGTGGGACGGTGTAGTCTg < 1:1178698/150‑1 (MQ=255) ggTGCAGGACCTCGCCCTGCCCAGAGACCATGGCTGCTC‑CTGGCAGCGGCGCCTGATCACCCGCCAGTGCCAACAGCGCTGGGCCGATATCAAGGCCAGCGCCCGGCATCTGCGCCACGGCCAGTTAGTGTGGGGCGGAGACGTCCGGGa > 2:1186560/1‑150 (MQ=255) GGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCATCCTCACCCGGCAGGGCGAACAGCGCTGGGCCGATATCAAGGCCAGCGCCCGGCATCTGGGCAACGGCCAGGTAGTGTGGGACGGTGTAGTCTgg < 1:990585‑M1/148‑1 (MQ=255) GGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCATCCTCACCCGGCAGGGCGAACAGCGCTGGGCCGATATCAAGGCCAGCGCCCGGCATCTGGGCAACGGCCAGGTAGTGTGGGACGGTGTAGTCTGGGa > 1:1376161‑M1/1‑150 (MQ=255) GGTGCAGGACCTGCCCCTGGCCAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCATCCTCACCCGGCAGGGCGAACAGCGCTGGGCCGATATCAAGGCCAGCGCCCGGCATCTGGGCAACGGCCAGGTAGTGTGGGACGGTGTAGTCTgg > 2:990585‑M1/1‑148 (MQ=255) cAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCATCCTCACCCGGCAGGGCGAACAGCGCTGGGCCGATATCAAGGCCAGCGCCCGGCATCTGGGCAACGGCCATGTAGTGTGGGACGGTGTAGTCTGGGACATc < 2:168879/150‑1 (MQ=255) ctggccctggccAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCATCCTCACCCGGCAGGGCGAACAGCGCTGGGCCGATATCAAGGCCAGCGCCCGGCATCTGGGCAACGGCCAGGTAGTGTGGGACGGTGTAGTCTGGGACATCACACAg > 2:200684/1‑150 (MQ=255) ctggccctggccAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCATCCTCACCCGGCAGGGCGAACAGCGCTGGGCCGATATCAAGGCCAGCGCCCGGCATCTGGGCAACGGCCAGGTAGTGTGGGACGGTGTAGTCTGGGACATCACACAg < 1:945087/150‑1 (MQ=255) ggccctggccAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCATCCTCACCCGGCAGGGCGAACAGCGCTGGGCCGATATCAAGGCCAGCGCCCGGCATCTGGGCAACGGCCAGGTAGTGTGGGACGGTGTAGTCTGGGACATCACACAggg < 1:897386/150‑1 (MQ=255) gccctggccAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCATCCTCACCCGGCAGGGCGAACAGCGCTGGGCCGATATCAAGGCCAGCGCCCGGCATCTGGGCAACGGCCa < 2:1335969/110‑1 (MQ=255) gccctggccAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCATCCTCACCCGGCAGGGCGAACAGCGCTGGGCCGATATCAAGGCCAGCGCCCGGCATCTGGGCAACGGCCa > 1:1335969/1‑110 (MQ=255) gccctggccAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCATCCTCACCCGGCAGGGCGAACAGCGCTGGGCCGATATCAAGGCCAGCGCCCGGCATCTGGGCAACGGCCAGGTAGTGTGGGACGGTGTAGTCTGGGACATCACACAggag > 2:299813/1‑148 (MQ=255) | TCCCCTATATCAGTGAGGGTAGCGAGGCTTTGGTGGGTTATGCACCCAGCGAGATCCAGCACCCGCAGATGGGCCTGCGCAACCTGGTGCACCCCGAGGACCGTGCTGATTATCACAGGGTGCAGGACCTGGCCCTGGCCAGCGACCAGGACTGGTC‑CTGGCAGGGGCGCATCCTCACCCGGCAGGGCGAACAGCGCTGGGCCGATATCAAGGCCAGCGCCCGGCATCTGGGCAACGGCCAGGTAGTGTGGGACGGTGTAGTCTGGGACATCACACAGGGC > NC_002947/497564‑497844 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |