Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	191,912	Δ1 bp	7.2%	coding (1606/2157 nt)	paxB ←	toxin secretion ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	191,912	0	C	.	7.2%	214.9 / 11.5	69	coding (1606/2157 nt)	paxB	toxin secretion ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (27/37);  new base . (3/2);  total (30/39)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.68e-01

GTAGCGGGCGCCGCTGACCAGGTTGTCACGCAAGGTGCCGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTCGGATAAGTGAAGTCGACC  >  NC_002947/191763‑192054                                                                                                                                                      |                                                                                                                                                gTAGCGGGCGCCGCTGACCAGGTTGTCACGCAAGGTGCCGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTc                                                                                                                                                 <  2:1418190/150‑1 (MQ=255)     cGGGCGCCGCTGACCAGGTTGTCACGCACGGTGCCGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAc                                                                                                                                             >  2:713277/1‑150 (MQ=255)        gcgcCGCTGACCAGGTTGTCACGCAAGGTGCCGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑Agg                                                                                                                                         <  1:97726/150‑1 (MQ=255)          gccgcTGACCAGGTTGTCACGCAAGGTGCCGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGcc                                                                                                                                       <  1:852010/150‑1 (MQ=255)               tGACCAGGTTGTCACGCAAGGTGCCGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCcgacga                                                                                                                                  <  1:888531/150‑1 (MQ=255)               tGACCAGGTTGTCACGCAAGGTGCCGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCcgacga                                                                                                                                  >  2:761738/1‑150 (MQ=255)               tGACCAGGTTGTCACGCAAGGTGCCGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCcgacga                                                                                                                                  <  1:926427/150‑1 (MQ=255)                gACCAGGTTGTCACGCAAGGTGCCGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGAt                                                                                                                                 <  1:801281/150‑1 (MQ=255)                    aGGTTGTCACGCAAGGTGCCGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGc                                                                                                                             >  2:118917/1‑150 (MQ=255)                          tCACGCAAGGTGCCGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGcc                                                                                                                       >  2:1164459/1‑150 (MQ=255)                                aaGGTGCCGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCg                                                                                                                                                >  1:928976/1‑120 (MQ=255)                                aaGGTGCCGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCg                                                                                                                                                <  2:928976/120‑1 (MQ=255)                                     gCCGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCtt                                                                                                            <  1:905632/150‑1 (MQ=255)                                      ccGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCATCAGCCAGCCACCATCGGCCGCTTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTg                                                                                                           <  1:175288/150‑1 (MQ=255)                                       cGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCACCGGCCT‑GTACGAGGCcgatgg                                                                                                                                  >  1:377449/1‑123 (MQ=255)                                       cGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCACCGGCCT‑GTACGAGGCcgatgg                                                                                                                                  <  2:377449/126‑4 (MQ=255)                                         gCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCGCCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCg                                                                                                        >  1:1008592/1‑150 (MQ=255)                                           cagcagCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGa                                                                                                      <  1:594327/150‑1 (MQ=255)                                                gcTGGATCTCCTGGGGCACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGc                                                                                                 >  2:1128998/1‑150 (MQ=255)                                                   ggATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGc                                                                                              <  2:953895/150‑1 (MQ=255)                                                   ggATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGc                                                                                              >  1:148495/1‑150 (MQ=255)                                                      tatCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGc                                                                                           <  2:1026200/150‑1 (MQ=255)                                                        tCCTGGGGGACGTAGCCGATGTTGTGGCGCTGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCAGAGCCGCTGCGGCCg                                                                                         >  1:1328450/1‑150 (MQ=255)                                                        tCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGCAC‑AGCCCGAGCATGAGTTTGTCCAGCGTGCCGGTTCCGGAGCCGCTGAGGACg                                                                                         >  2:727570/1‑150 (MQ=255)                                                        tCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATAGCTCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCg                                                                                         >  2:1267674/1‑150 (MQ=255)                                                          cTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCgat                                                                                       >  1:765916/1‑150 (MQ=255)                                                           tGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCgatg                                                                                      >  2:1319032/1‑150 (MQ=255)                                                             ggggACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCgatgat                                                                                    >  2:1393564/1‑150 (MQ=255)                                                                   gTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCga                                                                                        >  1:6097/1‑140 (MQ=255)                                                                   gTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCga                                                                                        <  2:6097/140‑1 (MQ=255)                                                                      gCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCtt                                                                           >  2:445929/1‑150 (MQ=255)                                                                              tgtgGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTtctc                                                                        >  2:694114/1‑145 (MQ=255)                                                                              tgtgGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTtctc                                                                        <  1:694114/145‑1 (MQ=255)                                                                              tgtgGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAg                                                                   <  2:289163/150‑1 (MQ=255)                                                                               gtgGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGGTCAATCTGCTGCGGCCGATGATGCCGACCTTCTCGCCAgg                                                                  >  1:784985/1‑150 (MQ=255)                                                                                tgGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAggg                                                                 <  2:616322/150‑1 (MQ=255)                                                                                    gcAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGa                                                             <  1:762311/150‑1 (MQ=255)                                                                                    gcAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGa                                                             <  1:761738/150‑1 (MQ=255)                                                                                             tGACATCGATCTGGCGACTGTCGACGACACAACCCCGCAGCGAGCCACCCTCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGt                                                    <  2:784985/150‑1 (MQ=255)                                                                                              gACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCGCCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGtt                                                   <  2:1008592/150‑1 (MQ=255)                                                                                              gACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGtt                                                   <  1:1036231/150‑1 (MQ=255)                                                                                              gACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGtt                                                   <  1:255553/150‑1 (MQ=255)                                                                                                 aTCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCa                                                                    >  2:1221744/1‑130 (MQ=255)                                                                                                 aTCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCa                                                                    <  1:1221744/130‑1 (MQ=255)                                                                                                  tCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGata                                               <  1:1319032/150‑1 (MQ=255)                                                                                                       cTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATAttctt                                          <  1:1055937/150‑1 (MQ=255)                                                                                                        tGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATAttcttc                                         <  2:814395/150‑1 (MQ=255)                                                                                                            gAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAggg                                     >  1:685796/1‑150 (MQ=255)                                                                                                                   gACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGt                                                    <  1:276971/128‑1 (MQ=255)                                                                                                                   gACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGt                                                    >  2:276971/1‑128 (MQ=255)                                                                                                                    aCGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTc                             <  1:1164459/150‑1 (MQ=255)                                                                                                                       ccATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTctgc                          <  1:445929/150‑1 (MQ=255)                                                                                                                        cATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTctgct                         >  1:1268339/1‑150 (MQ=255)                                                                                                                         aTCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTctgctg                        >  1:137084/1‑150 (MQ=255)                                                                                                                          tCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTctgc                          <  2:1044655/147‑1 (MQ=255)                                                                                                                          tCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTctgc                          >  1:1044655/1‑147 (MQ=255)                                                                                                                             aCCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTc                    <  2:148495/150‑1 (MQ=255)                                                                                                                             aCCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCACTGCGGCCGATGATGCCGACCTTCTCGGCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTc                    <  1:713277/150‑1 (MQ=255)                                                                                                                              ccAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCAGATGATGCCGACCTTCTCGCCAGGGCGGCTGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTCg                   <  1:1393564/150‑1 (MQ=255)                                                                                                                                agcagcGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTCGGa                 <  2:685796/150‑1 (MQ=255)                                                                                                                                agcagcGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTCGGa                 >  1:64424/1‑150 (MQ=255)                                                                                                                                agcagcGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTATGCTGGttgtga                 <  2:765916/150‑5 (MQ=255)                                                                                                                                        gCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGAAATACGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTCGGATAAGTGaa         <  1:727570/150‑1 (MQ=255)                                                                                                                                           atcgtCGGCCT‑GTACGAGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTCGGATAAGTGAAGTc      >  1:570800/5‑150 (MQ=255)                                                                                                                                              aTCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTCGGATAAGTGAAGTCGAc   >  1:718580/1‑150 (MQ=255)                                                                                                                                               tCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGTTGGGGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTCGGATAAGTGAAGTCGAcc  <  1:155189/150‑1 (MQ=255)                                                                                                                                               tCGGCCT‑GTACGAGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTCGGATAAGTGAAGTCGa    >  2:237712/1‑148 (MQ=255)                                                                                                                                               tCGGCCT‑GTACGAGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTCGGATAAGTGAAGTCGa    <  1:237712/148‑1 (MQ=255)                                                                                                                                                     tCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTCGGATaa              >  1:713236/1‑132 (MQ=255)                                                                                                                                                     tCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTCGGATaa              <  2:713236/132‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                GTAGCGGGCGCCGCTGACCAGGTTGTCACGCAAGGTGCCGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTCGGATAAGTGAAGTCGACC  >  NC_002947/191763‑192054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑