| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,846,339 | Δ1 bp | 12.0% | coding (588/1377 nt) | pfeS‑II → | histidine kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,846,339 | 0 | T | . | 12.0% | 140.3 / 15.3 | 50 | coding (588/1377 nt) | pfeS‑II | histidine kinase |
| Reads supporting (aligned to +/- strand): ref base T (25/18); new base . (3/3); total (29/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.82e-01 | |||||||||||
AATCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTGCGCGACCTGTCCCACGAAATGCG > NC_002947/1846190‑1846488 | aaTCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGAt < 2:1213449/150‑1 (MQ=255) tCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCa > 2:1289403/1‑150 (MQ=255) gCTTGCTGTGGCGCATTGTCACCAACCGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGA‑CAGCTGATCGGCCCGcc < 2:1143475/150‑1 (MQ=255) gcATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCa < 1:1421117/150‑1 (MQ=255) aTTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCAcc > 1:529896/1‑150 (MQ=255) tCACCAACGGCATCATCCCGGGGCTGTAAACCTTGCTGCTGTGCGTAGCCCTGTACCGCATGCCGACCGTGCCGCTGAACCAACTTCGCGAACAGGCCAATGCCTGGCCGCCCCATCCACGC‑CCAGCCCCCCTGGACTCCCGCACCACCg > 2:294799/1‑150 (MQ=255) aCCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGcc > 2:297912/1‑150 (MQ=255) atcCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGaa > 2:1010622/1‑150 (MQ=255) cccGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAAtt < 2:164476/150‑1 (MQ=255) ccGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCAGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTg > 2:443509/1‑150 (MQ=255) tGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGA‑CAGCTGATCGGc > 2:1011579/1‑103 (MQ=255) tGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGA‑CAGCTGATCGGc < 1:1011579/103‑1 (MQ=255) cTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGccc > 2:180341/1‑122 (MQ=255) cTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGccc < 1:180341/122‑1 (MQ=255) gttgtGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCtt > 2:408469/1‑150 (MQ=255) gttgtGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGCCATGACGAATTGGGCGAACTGGCGCGTGCCtt < 2:524137/150‑1 (MQ=255) ttgtGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCttt > 2:1200263/1‑150 (MQ=255) gtctgtCGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGCGGAGTCCAATGGCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCTGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGa < 1:1156136/147‑1 (MQ=255) gtAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAg > 2:879585/1‑150 (MQ=255) tAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGa < 2:900503/150‑1 (MQ=255) tAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGa < 1:408469/150‑1 (MQ=255) aGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGAt < 1:1289403/150‑1 (MQ=255) gCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCTCACCATCGc > 1:1087209/1‑103 (MQ=255) gCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGc < 2:1087209/103‑1 (MQ=255) gCCTGTACCGCATGCTGATCGTGACGCTGAACCAACTGCGCGAGCTGTCCAATGCCTAGGAGGCCGATCAGGTG‑TCGGCCCGCCTTGACTCGGGCACCATCGCTCCGCATGTCGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATgg > 2:125197/1‑150 (MQ=255) gCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGc < 1:1077180/150‑1 (MQ=255) tGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTggg < 1:328955/106‑1 (MQ=255) tGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTggg > 2:328955/1‑106 (MQ=255) gTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACg > 1:391870/1‑150 (MQ=255) tGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGa < 1:106342/121‑1 (MQ=255) tGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGa > 2:106342/1‑121 (MQ=255) gCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGAGCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCTCGGGAGGACGGATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACggg > 1:242860/1‑149 (MQ=255) cGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTgg < 1:1200263/150‑1 (MQ=255) cGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATAGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTgg > 1:217941/1‑150 (MQ=255) gCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGc > 2:1255073/1‑150 (MQ=255) ccAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGt < 1:229448/103‑1 (MQ=255) ccAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGt > 2:229448/1‑103 (MQ=255) aCTTCGCGAGCAGGCCAATGCCTGGAGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATgcagca < 1:653664/150‑1 (MQ=255) ttCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATgcagcagc > 1:1098988/1‑150 (MQ=255) atcgCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCAGCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGtt > 2:426932/4‑150 (MQ=255) gAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTtg > 1:1294224/1‑150 (MQ=255) aGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTtgctgc > 1:747009/1‑150 (MQ=255) aGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTtgctgc < 1:1010622/150‑1 (MQ=255) gCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTgcgc < 2:529896/150‑1 (MQ=255) cAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTGCGCGa > 2:308662/1‑150 (MQ=255) cAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATCGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTGCGCGa > 2:452585/1‑150 (MQ=255) gagtccaggCGGGCCGA‑CAGCTGATCGGCCCGCCAGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTGCGCGAc > 2:629503/8‑150 (MQ=38) gCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTg < 2:1050956/115‑1 (MQ=255) gCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTg > 1:1050956/1‑115 (MQ=255) gggCCGA‑CAGCTGATCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTGCGCGACCTGTCCCAc > 2:518802/1‑149 (MQ=255) gggCCGA‑CAGCTGATCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTGCGCGACCTGTCCCAc < 1:518802/149‑1 (MQ=255) tCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTGCGCGACCTGTCCCACGAAATgcg > 2:671336/1‑150 (MQ=255) tCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTCCCTTTGACCAGATGACCGAGCGGCCGCAAGGGACGGTGGCTATGCAGCAGCAGCTGATGCGCGACCTGTCCCACGaaagggg > 2:254198/1‑146 (MQ=255) | AATCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTGCGCGACCTGTCCCACGAAATGCG > NC_002947/1846190‑1846488 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |