| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,143,613 | Δ1 bp | 8.3% | coding (494/1539 nt) | rbsA‑II → | ribose ABC transporter ‑ ATP‑binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,143,613 | 0 | T | . | 8.3% | 147.8 / 7.6 | 48 | coding (494/1539 nt) | rbsA‑II | ribose ABC transporter ‑ ATP‑binding subunit |
| Reads supporting (aligned to +/- strand): ref base T (18/26); new base . (2/2); total (20/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.85e-01 | |||||||||||
CTCTCAGCAGCGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGCGTGGCCCTGGTGCAGCGTGAGGTGGAGCGTTTGCTGCGGATCGTCCAGCGCCTGCGTGACGATGGCCTGGCGATCGTCTACATCTCCCATTACCTGCAGGAAATCGAGGCCCTGTGCGACCGCGTGACGG > NC_002947/3143467‑3143759 | ctctCAGCAGCGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGGGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCCGGTGtt < 2:1203999/150‑1 (MQ=255) tcAGCAGCGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTcga < 2:111935/150‑1 (MQ=255) gcagcagCGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGAAACAGATGGTGCAGATAGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGG‑GTTcgtc > 2:975553/2‑148 (MQ=255) cgAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGAAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGG‑GTTCGTCGAAcaccag < 1:975553/150‑6 (MQ=255) gAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGc > 1:291677/1‑150 (MQ=255) cgTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGCGTGGCCCTg < 1:971743/150‑1 (MQ=255) gTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGCGTGGCCCTgg < 1:161933/150‑1 (MQ=255) tCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGCGTGGCCCTGGt < 1:1364511/150‑1 (MQ=255) gctgGACGACTACTTCGGCCTGCACCTGCCGGCTAACGCTGGGAGCTTGGTTCGGAGAAGTGCCGAGCAACAGATGGTAGACTTTTGCTGCCCGCTGTTTATCAAACCGCGTGTGCTGGTGTTCGACGAACCCGGCGTGGCCCGGGTGCa > 1:1391575/1‑150 (MQ=255) tgGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGCGTGGCCCTGGTGCAGc > 1:210483/1‑150 (MQ=255) gacgacTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGCGTGGCCCTGGTGCAGCGt > 1:1307371/1‑150 (MQ=255) gacTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGG‑GTTCGTCGAAcac < 2:1237117/125‑3 (MQ=255) gacTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGG‑GTTCGTCGAAcac > 1:1237117/1‑123 (MQ=255) ctTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGCGt > 2:1125322/1‑126 (MQ=255) ctTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGCGt < 1:1125322/126‑1 (MQ=255) ttCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGCGTGGCCCTGGTGCAGCGTGAGGTGGAg > 2:1049658/1‑150 (MQ=255) tCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGCGTGGCCCTGGTGCAGCGTGAGGTGGAGc < 2:389421/150‑1 (MQ=255) gCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGCGTGGCCCTGGTGCAGCGTGAGGTGGAGCGtt < 1:971607/150‑1 (MQ=255) cTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGCGTGGCCCTGGTGCAGCGTGAGGTGGAGCGTTtg > 2:1387208/1‑150 (MQ=255) cGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCACGTGTGCTGGTGTTCGACGAACCCAGCGTGGCCCTGGTGCAGCGTGAGGTGGAGCGTTtgctg < 1:298376/150‑1 (MQ=255) gACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGCGTGGCCCTGGTGCAGCGTGAGGTGGAGCGTTtgctgc > 2:834918/1‑150 (MQ=255) aCTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGCGTGGCCCTGGTGCAGCGTGAGGTGGAGCGTTTGCTGCg < 2:672773/150‑1 (MQ=255) gCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGCGTGGCCCTGGTGCAGCGTGAGGTGGAGCGTTTGCTGCGGATCGTc < 1:84018/150‑1 (MQ=255) aTGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGCGTGGCCCTGGTGCAGCGTGAGGTGGAGCGTTTGCTGCGGATCGTCCAgc < 2:1307371/150‑1 (MQ=255) tGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGCGTGGCCCTGGTGCAGCGTGAGGTGGAGCGTTTGCTGCGGATCGTCCAGCGCCTGc < 1:1375506/150‑1 (MQ=255) gATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAAc < 1:652676/89‑1 (MQ=255) gATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAAc > 2:652676/1‑89 (MQ=255) aTCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGCGTGGCCCTGGTGCAGCGTGAGGTGGAGCGTTTGCTGCGGa < 1:697806/133‑1 (MQ=255) aTCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGCGTGGCCCTGGTGCAGCGTGAGGTGGAGCGTTTGCTGCGGa > 2:697806/1‑133 (MQ=255) tCGTTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGCGTGGCCCTGGTGCAGCGTGAGGTGGAGCGTTtgctgc < 2:516509/129‑1 (MQ=255) tCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGCGTGGCCCTGGTGCAGCGTGAGGTGGAGCGTTtgctgc > 1:516509/1‑129 (MQ=255) gTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGCGTGGCCCTGGTGCAGCGTGAGGTg > 1:632420/1‑113 (MQ=255) gTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACAAACCCAGCGTGGCCCTGGTGCAGCGTGAGGTg < 2:632420/113‑1 (MQ=255) aaCTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGCGTGGCACTGGTGCAGCGTGAGGTGGAGCGTTTGCTGCGGATCGTCCAGCGCCTGCGTGACGATg < 1:480739/150‑1 (MQ=255) aCTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGCGTGGCCCTg < 1:142859/94‑1 (MQ=255) aCTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGCGTGGCCCTg > 2:142859/1‑94 (MQ=255) cTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGCGTGGCCCTGGTGCAGCGTGAGGTGGAGCGTTTGCTGCGGATCGTCCAGCGCCTGCGTGACGATGGc < 1:647946/150‑1 (MQ=255) gAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGCGTGGCCCTGGTGCAGCGTGAGGTGGAGCGTTTGCTGCGGATCGTCCAGCGCCTGCGTGACGATGGCCTGGCGATCGTc > 2:880499/1‑150 (MQ=255) aTGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGCGTGGCCCTGGTGCAGCGTGAGGTGGAGCGTTTGCTGCGGATCGTCCAGCGCCTGCGTGACGATGGCCTGGCGATCGTCTACATCTcc < 1:913471/150‑1 (MQ=255) gTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGCGTGGCCCTGGTGCAGCGTGAGGTGGAGCGTTTGCTGCGGATCGTCCAGCGCCTGCGTGACGATGGCCTGGCGATCGTCTACATCTCCCATTACCTGCAg < 2:1220601/150‑1 (MQ=255) gcgcgcTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGCGTGGCCCTGGTGCAGCGTGAGGTGGAGCGTTTGCTGCGGATCGTCCAGCGCCTGCGTGACGATGGCCTGGCGATCGTCTACATCTCCCATTACCTGCAGGaaa < 1:701369/150‑1 (MQ=255) gctgctGATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGCGTGGCCCTGGTGCAGCGTGAGGTGGAGCGTTTGCTGCGGATCGTCCAGCGCCTGCGTGACGATGGCCTGGCGATCGTCTACATCTCCCATTACCTGCAGGAAATCGa < 2:291677/150‑1 (MQ=255) ctgctgATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGCGTGGCCCTGGTGCAGCGTGAGGTGGAGCGTTTGCTGCGGATCGTCCAGCGCCTGCGTGACCATGGCCTGGCGTTCGTCTACATCTCCCATTACCTGCAGGAAATCGAg < 1:983508/150‑1 (MQ=255) gcggATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGCGTGGCCCTGGTGCAGCGTGAGGTGGAGCGTTTGCTGCGGATCGTCCAGCGCCTGCGTGACGATGGCCTGGCGATCGTCTACATCTCCCATTACCTGCAGGAAATCGAGGc > 1:1425874/4‑150 (MQ=255) aaaCCGCGTGTGCTGGTGTTCGACGAACCCAGCGTGGCCCTGGTGCAGCGTGAGGTGGAGCGTTTGCTGCGGATCGTCCAGCGCCTGCGTGACGATGGCCTGGCGATCGTCTa > 2:382023/1‑113 (MQ=255) aaaCCGCGTGTGCTGGTGTTCGACGAACCCAGCGTGGCCCTGGTGCAGCGTGAGGTGGAGCGTTTGCTGCGGATCGTCCAGCGCCTGCGTGACGATGGCCTGGCGATCGTCTa < 1:382023/113‑1 (MQ=255) tgCTGGTGTTCGACGAACCCAGCGTGGCCCTGGTGCAGCGTGAGGTGGAGCGTTTGCTGCGGATCGTCCAGCGCCTGCGTGACGATGGCCTGGCGATCGTCTACATCTCCCATTACCTGCAGGAAATCGAGGCCCTGTGCGACCGCGTGa > 1:336460/1‑150 (MQ=255) tGGTGTTCGACGAACCCAGCGTGGCCCTGGTGCAGCGTGAGGTGGAGCGTTTGCTGCGGATCGTCCAGCGCCTGCGTGACGATGGCCTGGCGATCGTCTACATCTCCCATTACCTGCAGGAAATCGAGGCCCTGTGCGACCGCGTGACgg > 1:1193081/1‑150 (MQ=255) | CTCTCAGCAGCGCGAGGCCGCGCGTCTGCTGGACGACTACTTCGGCCTGCGACTGCCGGCGAATGCGCTGATCGGTGAACTGAGCAGCGCCGAGCAACAGATGGTGCAGATCGTCCGCGCGCTGCTGATCAAACCGCGTGTGCTGGTGTTCGACGAACCCAGCGTGGCCCTGGTGCAGCGTGAGGTGGAGCGTTTGCTGCGGATCGTCCAGCGCCTGCGTGACGATGGCCTGGCGATCGTCTACATCTCCCATTACCTGCAGGAAATCGAGGCCCTGTGCGACCGCGTGACGG > NC_002947/3143467‑3143759 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |