| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,570,230:1 | +A | 7.9% | coding (70/861 nt) | nnrD ← | ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,570,230 | 1 | . | A | 7.9% | 235.3 / 15.3 | 63 | coding (70/861 nt) | nnrD | ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase |
| Reads supporting (aligned to +/- strand): ref base . (28/30); new base A (3/2); total (31/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.30e-01 | |||||||||||
TCGGGCCGGGTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCCCATCCATGTCCG > NC_002947/5570085‑5570371 | tCGGGCCGGGTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGc > 2:129247/1‑150 (MQ=255) aCGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTACTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTc > 1:373425/1‑150 (MQ=255) gACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACgct > 2:329667/1‑150 (MQ=255) gACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACgct > 2:1082849/1‑150 (MQ=255) ccGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGc > 2:922438/1‑150 (MQ=255) cgccgcAACGCAGGGCGGCTTCGGCACTGAGCCACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTATATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAAtt < 1:23673/150‑1 (MQ=255) cgccgcAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAAtt < 1:922438/150‑1 (MQ=255) cgcAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATtgtg < 2:1414265/150‑1 (MQ=255) cgcAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATtgtg < 1:1189031/150‑1 (MQ=255) cAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTggg < 1:1216898/150‑1 (MQ=255) aCTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGt > 1:817908/1‑150 (MQ=255) cTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTc > 2:1255802/1‑150 (MQ=255) tGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTgg > 2:330205/1‑127 (MQ=255) tGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTgg < 1:330205/127‑1 (MQ=255) cAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCG‑GCACGGCAGGc > 2:1178691/1‑92 (MQ=255) cAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCG‑GCACGGCAGGc < 1:1178691/92‑1 (MQ=255) acacAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTgg > 1:1362805/1‑132 (MQ=255) acacAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTgg < 2:1362805/132‑1 (MQ=255) cacaGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCAt < 1:129247/150‑1 (MQ=255) acaGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTg > 1:102595/1‑138 (MQ=255) acaGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTg < 2:102595/138‑1 (MQ=255) aGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATtgtg > 2:876125/1‑116 (MQ=255) aGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATtgtg < 1:876125/116‑1 (MQ=255) aGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATggg < 1:1082849/150‑1 (MQ=255) aGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATggg < 2:139016/150‑1 (MQ=255) gcCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTc < 2:817908/150‑1 (MQ=255) gcCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCG‑GCACGGCAGGCg < 2:145970/84‑1 (MQ=255) gcCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCG‑GCACGGCAGGCg > 1:145970/1‑84 (MQ=255) ccTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCa > 2:71973/1‑150 (MQ=255) tCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCaaa < 1:11180/150‑1 (MQ=255) cGGTACCCAGGTCGCCCCCGACCACCAGTACATGACCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAgg > 2:140123/1‑150 (MQ=255) ccAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGa > 1:164718/1‑93 (MQ=255) ccAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGa < 2:164718/93‑1 (MQ=255) ccAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCaa < 1:264705/150‑1 (MQ=255) ccAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCaa < 2:1291119/150‑1 (MQ=255) ccAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCaa > 1:634356/1‑150 (MQ=255) ccAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCACGGGGTCAAAGGCTc > 1:626809/1‑147 (MQ=255) ccAGGTCGCCCCCGACCACCAGCACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTc < 2:626809/147‑1 (MQ=255) cAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAg < 2:762246/93‑1 (MQ=255) cAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAg > 1:762246/1‑93 (MQ=255) cccccGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATggg < 2:765912/128‑1 (MQ=255) cccccGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATggg > 1:765912/1‑128 (MQ=255) cccccGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGc > 2:1323349/1‑150 (MQ=255) caccaGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATtat < 1:1255802/150‑1 (MQ=255) accaGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATtata < 1:1323349/150‑1 (MQ=255) ccaGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATAc > 2:783374/1‑150 (MQ=255) ccaGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATAc > 1:33859/1‑150 (MQ=255) ccAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTgg < 1:1223227/74‑1 (MQ=255) ccAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTgg > 2:1223227/1‑74 (MQ=255) tggCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAGTTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATCAGCACCTGAGCCCGtttt > 2:241323/3‑150 (MQ=255) cTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAgg < 2:469560/105‑1 (MQ=255) cTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAgg > 1:469560/1‑105 (MQ=255) cTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCAc < 2:1005876/133‑1 (MQ=255) cTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCAc > 1:1005876/1‑133 (MQ=255) cTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTAttgcct < 1:329667/150‑1 (MQ=255) tttGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTAttgcctt < 1:71973/150‑1 (MQ=255) gCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCccatc < 2:33859/150‑1 (MQ=255) gcgcCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCa < 2:60667/89‑1 (MQ=255) gcgcCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCa > 1:60667/1‑89 (MQ=255) ccgcCTGCCG‑GCACGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCCCATCCATg > 2:178994/2‑150 (MQ=255) ccTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCt < 2:397070/120‑1 (MQ=255) ccTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCt > 1:397070/1‑120 (MQ=255) cTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCCCATCCATGTCCg > 1:1376416/1‑150 (MQ=255) | TCGGGCCGGGTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGC‑CGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCCCATCCATGTCCG > NC_002947/5570085‑5570371 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |