Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,997,245:1	+G	24.5%	intergenic (‑158/+42)	PP_5252 ← / ← PP_5253	hypothetical protein/arylesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,997,245	1	.	G	24.5%	127.9 / 52.2	53	intergenic (‑158/+42)	PP_5252/PP_5253	hypothetical protein/arylesterase
Reads supporting (aligned to +/- strand):  ref base . (14/26);  new base G (6/7);  total (20/33)
Fisher's exact test for biased strand distribution p-value = 5.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

CGACTAGGGGCCGGCAGGGGGACTGCGAAGCATGCCGGTTGATGCAAGGCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGCCCCGGCATACACCTTCAGCTCGGCGCCACGAATCAG  >  NC_002947/5997097‑5997394                                                                                                                                                      |                                                                                                                                                      cGACTAGGGGCCGGCAGGGGGACTGCGAAGCATGCCGGTAGATTCAAGGCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACa‑a                                                                                                                                                      <  1:1084678/150‑1 (MQ=255)    cTAGGGGCCGGCAGGGGGACTGCGAAGCATGCCGGTTGATGCAAGGCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGt                                                                                                                                                   <  1:909528/150‑1 (MQ=255)    cTAGGGGCCGGCAGGGGGACTGCGAAGCATGCCGGTTGATGCAAGGCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGt                                                                                                                                                   <  2:1420973/150‑1 (MQ=255)      aGGGGCCGGCAGGGGGACTGCGAAGCATGCCGGTTGATGCAAGGCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCt                                                                                                                                                 <  1:508621/150‑1 (MQ=255)       ggggCCGGCAGGGGGACTGCGAAGCATGCCGGTTGATGCAAGGCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTg                                                                                                                                                <  2:1401067/150‑1 (MQ=255)          gCCGGCAGGGGGACTGGGACGCATGCCGGTTGATGCAAGGCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAg                                                                                                                                             <  2:936612/150‑1 (MQ=255)            cGGCAGGGGGACTGCGAAGCATGCCGGTTGATGCAAGGCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGa                                                                                                                                           <  1:765558/150‑1 (MQ=255)              gCAGGGGGACTGCGAAGCATGCCGGTTGATGCAAGGCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGACGT‑TGTAGGAGc                                                                                                                                         <  2:851854/150‑1 (MQ=255)              gCAGGGGGACTGCGAAGCATGCCGGTTGATGCAAGGCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGACGT‑TGTAGGAGc                                                                                                                                         <  2:1297706/150‑1 (MQ=255)              ccAGGGGGACTGCGAAGCATGCCGGTTGATGCAAGGCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGCCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGc                                                                                                                                         <  1:111164/149‑1 (MQ=255)                      aCTGCGAAGCATGCCGGTTGATGCAAGGCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTAc                                                                                                                                 <  2:912042/150‑1 (MQ=255)                        tGCGAAGCATGCCGGTTGATGCAAGGCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTAccc                                                                                                                               >  1:506120/1‑150 (MQ=255)                                    cGGTTGATGCAAGGCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTcc                                                                                                                   <  1:863913/150‑1 (MQ=255)                                      gTTGATGCAAGGCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGACGT‑TGTAGGAGCGGGTTTACCCGCGaaga                                                                                                                        <  1:1136961/143‑3 (MQ=255)                                      gTTGATGCAAGGCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGACGT‑TGTAGGAGCGGGTTTACCCGCGaaga                                                                                                                        >  2:1136961/1‑141 (MQ=255)                                                ggCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGc                                                                                                       <  2:292923/150‑1 (MQ=255)                                                ggCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGc                                                                                                       <  2:1348488/150‑1 (MQ=255)                                                    tGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTg                                                                                                                                                <  2:393140/105‑1 (MQ=255)                                                    tGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTg                                                                                                                                                >  1:393140/1‑105 (MQ=255)                                                         ttGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAAc                                                                                              >  1:993566/1‑150 (MQ=255)                                                            aaTGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGcc                                                                                           >  2:1054444/1‑150 (MQ=255)                                                                   gtgCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGt                                                                                    <  1:66811/150‑1 (MQ=255)                                                                       ggTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTAc                                                                                                                                 <  1:806269/101‑1 (MQ=255)                                                                       ggTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTAc                                                                                                                                 >  2:806269/1‑101 (MQ=255)                                                                        gTGGGTTGAAGAAGCTTCGACCTGGTCAAGCTTTTAGCTGCGTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCa                                                                               <  1:1161399/150‑1 (MQ=38)                                                                                       ttAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCa                                                                <  1:405136/150‑1 (MQ=255)                                                                                          gCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGa                                                             <  1:1054444/150‑1 (MQ=255)                                                                                                gTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACt                                                       <  2:993566/150‑1 (MQ=255)                                                                                                     gCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCaaa                                                  >  2:1293173/1‑150 (MQ=255)                                                                                                      cTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCaaaa                                                 <  2:506120/150‑1 (MQ=255)                                                                                                                     gccggccTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGCCCCg                                  >  1:332045/1‑150 (MQ=255)                                                                                                                          ccTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGCCCCGGCATa                             >  2:1178510/1‑150 (MQ=255)                                                                                                                                  cgGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATACAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGCCCCGGCATACACCTTCa                     <  1:536025/150‑1 (MQ=255)                                                                                                                                         aCCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGCCCCGGCATACACCTTCAGCTCGgc              <  2:879353/150‑1 (MQ=255)                                                                                                                                         aCCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGCCCCGGCATACACCTTCAGCTCGgc              >  1:291531/1‑150 (MQ=255)                                                                                                                                           ccGCTCCTACAGACGT‑TGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCa                                                                >  1:63716/1‑98 (MQ=37)                                                                                                                                           ccGCTCCTACAGACGT‑TGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCa                                                                <  2:63716/98‑1 (MQ=37)                                                                                                                                           ccGCTCCTACAGACGT‑TGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGCCCCGGCATac                            <  1:647379/134‑1 (MQ=255)                                                                                                                                           ccGCTCCTACAGACGT‑TGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGCCCCGGCATac                            >  2:647379/1‑134 (MQ=255)                                                                                                                                           ccGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTgcg                                          <  2:615381/120‑1 (MQ=255)                                                                                                                                           ccGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTgcg                                          >  1:820132/1‑120 (MQ=255)                                                                                                                                           ccGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTgcg                                          <  2:820132/120‑1 (MQ=255)                                                                                                                                           ccGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTACCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTgcg                                          >  1:615381/1‑120 (MQ=255)                                                                                                                                             gCTCCTACAGACGT‑TGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTAc                                                                                                          >  1:1282600/1‑54 (MQ=14)                                                                                                                                             gCTCCTACAGACGT‑TGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTAc                                                                                                          <  2:1282600/54‑1 (MQ=14)                                                                                                                                             gCTCCTACAGACGT‑TGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTc                                                                                   <  2:527564/77‑1 (MQ=25)                                                                                                                                             gCTCCTACAGACGT‑TGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTc                                                                                   >  1:527564/1‑77 (MQ=25)                                                                                                                                             gCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGCCCCGGCATACACCTTCAGCTCGGCGCCa          >  1:518428/1‑150 (MQ=255)                                                                                                                                              cTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTATCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGCCCCGGCATacac                          <  1:385796/133‑1 (MQ=255)                                                                                                                                              cTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGCCCCGGCATacac                          >  2:385796/1‑133 (MQ=255)                                                                                                                                              cTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGCCCCGGCATACACCTTCAGCTCGGCGCCAc         <  1:1293173/150‑1 (MQ=255)                                                                                                                                               tCCTACAGACGT‑TGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGCCCCGGCATACACCTTCAGCTCGCCGCCACg        >  2:973751/1‑150 (MQ=255)                                                                                                                                                    cA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGCCCCGGCATACACCTTCAGCTCGGCGCCAc         >  2:635877/1‑144 (MQ=255)                                                                                                                                                    cA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGCCCCGGCATACACCTTCAGCTCGGCGCCAc         <  1:635877/144‑1 (MQ=255)                                                                                                                                                     a‑aCGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGCCCCGGCATACACCTTCAGCTCGGCGCCACGAATCAg  >  2:860977/1‑150 (MQ=255)                                                                                                                                                      |                                                                                                                                                      CGACTAGGGGCCGGCAGGGGGACTGCGAAGCATGCCGGTTGATGCAAGGCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGCCCCGGCATACACCTTCAGCTCGGCGCCACGAATCAG  >  NC_002947/5997097‑5997394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑