| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 924,324:1 | +A | 9.7% | coding (872/2169 nt) | PP_0804 ← | protein secretion ABC transporter permease/ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 924,324 | 1 | . | A | 9.7% | 221.4 / 19.8 | 62 | coding (872/2169 nt) | PP_0804 | protein secretion ABC transporter permease/ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base . (33/23); new base A (3/3); total (36/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.08e-01 | |||||||||||
CCGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGGCGCGCTTGC > NC_002947/924182‑924471 | ccGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCt < 1:229531/150‑1 (MQ=255) cAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGTCCAGCACCGCGA‑CGGTGCTGGCCga < 2:18559/150‑2 (MQ=255) cAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGt > 2:136692/1‑150 (MQ=255) cAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGt > 2:743550/1‑150 (MQ=255) aGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGGACCGTCG‑CGGTGCTGGACGTg > 1:73718/1‑150 (MQ=255) gcgcCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTTAt < 1:945403/150‑1 (MQ=255) cggtctgtgCAAGCACCCCGCGCAGCAGCATGGCCAGCATCGCGACCCGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAAAAGGAACAGCAGGTAGACCGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGAGCa < 1:230855/145‑1 (MQ=255) ccTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCTGCGCGACCAGCGGGATGCACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACCGCAGGAAGAACGCCAGGTGGGCCAGCATCGTCG‑CGGTGCTGGACGTGAttcg > 2:314280/1‑147 (MQ=255) ccTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGATCAg < 1:1163955/150‑1 (MQ=255) tttGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGTCCAGCACCGCGA‑CGGTGCTggcc > 2:412476/1‑138 (MQ=255) tttGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGTCCAGCACCG‑CGACGGTGCTggcc < 1:412476/140‑3 (MQ=255) tgCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACGAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGATCAgagatcg > 2:230855/1‑144 (MQ=255) gcaagcaaCCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGATCAGGTCGCgg > 2:1112025/1‑150 (MQ=255) caagcaaCCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGATCAGGTCGCGGa < 1:143744/150‑1 (MQ=255) aagcaaCCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGATCAGGTCGCGGAt > 1:1368114/1‑150 (MQ=255) gcaaCCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGATCAGGTCGCGGATCg > 2:1083549/1‑150 (MQ=255) cccGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGATCAGGTCGCGGATCGACTc > 1:697787/1‑150 (MQ=255) agcaCCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGTTGGACGTGATCAGGTCGCGGATCGACTCCAGCTcg > 1:1226355/1‑150 (MQ=255) ccATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCG‑CGACGGTGCTgg > 1:403245/1‑114 (MQ=255) ccATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCG‑CGACGGTGCTgg < 2:403245/114‑1 (MQ=255) cAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGcc > 2:1198717/1‑150 (MQ=255) gCGGGATGAACACCAGTACGCCGCCAATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCgg > 1:975855/1‑150 (MQ=255) ggATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTgg > 2:521048/1‑150 (MQ=255) aTGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGAc < 2:697787/150‑1 (MQ=255) tGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGAc < 2:6708/89‑1 (MQ=255) tGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGAc > 1:6708/1‑89 (MQ=255) acacCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTgg < 1:1083549/150‑1 (MQ=255) acacCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTgg > 2:368209/1‑150 (MQ=255) cacCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTggg < 1:521048/150‑1 (MQ=255) cAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCg > 2:511571/1‑150 (MQ=255) cAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGATCGCACAACTCCGAGATGAACCGGCCGGTGGACGTGTGCCg > 2:1016837/1‑150 (MQ=255) gccgccGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGaa > 2:390033/1‑123 (MQ=255) gccgccGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGaa < 1:390033/123‑1 (MQ=255) cgccgATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGt > 1:754926/1‑150 (MQ=255) ccAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTgcgcaggcg > 2:338404/1‑150 (MQ=255) cAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTgcgcaggcgc > 2:35250/1‑150 (MQ=255) cAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTgcgcaggcgc > 2:1099918/1‑150 (MQ=255) aGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGa < 2:861809/66‑1 (MQ=255) aGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGa > 1:861809/1‑66 (MQ=255) aGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGATGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTAAGCCGCACCGAGTTgcgcaggcgca > 2:83718/1‑150 (MQ=255) gAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGGGGACTTGGGCCGCACCGAGTTgcgcaggcgcag > 2:327081/1‑150 (MQ=255) acGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGa < 1:243210/103‑1 (MQ=255) acGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGa > 2:243210/1‑103 (MQ=255) gaacaggaacagCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATg > 2:928514/1‑150 (MQ=255) agGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTcgc < 1:442093/73‑1 (MQ=255) agGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTcgc > 2:442093/1‑73 (MQ=255) agGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAgg < 2:652883/150‑1 (MQ=255) ggAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCCCCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAggg > 1:653868/1‑149 (MQ=255) ggAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGt < 1:1112025/150‑1 (MQ=255) gaaCGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTgg > 2:1040671/1‑101 (MQ=255) gaaCGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTgg < 1:1040671/101‑1 (MQ=255) gCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCAccc < 1:1016837/150‑1 (MQ=255) aGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCTCTCACCCGc < 1:743550/150‑1 (MQ=255) gtccAGCACCGCGA‑CGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTg < 1:1332730/80‑1 (MQ=38) gtccAGCACCG‑CGACGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTg > 2:1332730/3‑82 (MQ=38) gCCAGCACCGTCG‑CGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCg < 1:368209/150‑1 (MQ=255) ccAGCACCGTCG‑CGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGGTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCgg > 1:36413/1‑150 (MQ=255) ccAGCACCGTCG‑CGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGAGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCgg > 2:851173/1‑150 (MQ=255) ccAGCACCG‑CGACGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAg > 1:810530/1‑145 (MQ=255) ccAGCACCG‑CGACGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAg < 2:810530/145‑1 (MQ=255) gCACCGTCG‑CGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGAGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGgcgc < 1:851173/150‑1 (MQ=255) tCG‑CGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGGCGCGCTTGc < 1:35250/150‑1 (MQ=255) | CCGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGGCGCGCTTGC > NC_002947/924182‑924471 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |