Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,899,207:1 +C 9.1% coding (1389/1413 nt) PP_2552 → DOPA decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,899,2071.C 9.1% 203.0 / 15.5 55coding (1389/1413 nt)PP_2552DOPA decarboxylase
Reads supporting (aligned to +/- strand):  ref base . (27/23);  new base C (2/3);  total (29/26)
Fisher's exact test for biased strand distribution p-value = 6.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.08e-01

GCATACCAAGGGCTGGGCCGAGCGGCTGAATGCATCCGGCGCTGCTTATGTGACGCCGGCTACACTGGACGGGCGGTGGATGGTGCGGGTTTCGATTGGTGCGCTGCCGACCGAGCGGGGGGATGTGCAGCGGCTGTGGGCACGT‑CTGCAGGACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCGGGCTTGCCCGCGAAGGGGCTGCAAAGCAGCCCCTGCAATCTATCAGTGGCTCACCGCCTGAAAGCTACCTTTACGGCTGGCTTCATAGGCTGCCTT  >  NC_002947/2899063‑2899352
                                                                                                                                                 |                                                                                                                                                 
gcATACCAAGGGCTGGGCCGAGCGGCTGAATGCATCCGGCGCTGCTTATGTGACGCCGGCTACACTGGACGGGCGGTGGATGGTGCGGGTTTCGATTGGTGCGCTGCCGACCGAGCGGGGGGATGTGCAGCGGCTGTGGGCACGT‑CTGCa                                                                                                                                              <  2:228336/150‑1 (MQ=255)
  aTACCAAGGGCTGGGCCGAGCGGCTGAATGCATCCGGCGCTGCTTATGTGACGCCGGCTACACTGGACGGGCGGTGGATGGTGCGGGTTTCGATTGGTGCGCTGCCGACCGAGCGGGGGGATGTGCAGCGGCTGTGGGCACGT‑CTGCAgg                                                                                                                                            >  2:664518/1‑150 (MQ=255)
   tACCAAGGGCTGGGCCGAGCGGCTGAATGCATCCGGCGCTGCTTATGTGACGCCGGCTACACTGGACGGGCGGTGGATGGTGCGGGTTTCGATTGGTGCGCTGCCGACCGAGCGGGGGGATGTGCAGCGGCTGTGGGCACGT‑CTGCAGGa                                                                                                                                           >  2:963837/1‑150 (MQ=255)
      cAAGGGCTGGGCCGAGCGGCTGAATGCATCCGGCGCTGCTTATGTGACGCCGGCTACACTGGACGGGCGGTGGATGGTGCGGGTTTCGATTGGTGCGCTGCCGACCGAGCGGGGGGATGTGCAGCGGCTGTGGGCACGT‑CTGCAGGACGt                                                                                                                                        >  2:236208/1‑150 (MQ=255)
       aaGGGCTGGGCCGAGCGGCTGAATGCATCCGGCGCTGCTTATGTGACGCCGGCTACACTGGACGGGCGGTGGATGGTGCGGGTTTCGATTGGTGCGCTGCCGACCGAGCGGGGGGATGTGCAGCGGCTGTGGGCACGTCCTGCA‑GACGTg                                                                                                                                       <  2:1270309/150‑1 (MQ=255)
        aGGGCTGGGCCGAGCGGCTGAATGCATCCGGCGCTGCTTATGTGACGCCGGCTACACTGGACGGGCGGTGGATGGTGCGGGTTTCGATTGGTGCGCTGCCGACCGAGCGGGGGGATGTGCAGCGGCTGTGGGCACGTCCTGCA‑GACGTgc                                                                                                                                      <  1:899887/150‑2 (MQ=255)
         gggCTGGGCCGAGCGGCTGAATGCATCCGGCGCTGCTTATGTGACGCCGGCTACACTGGACGGGCGGCGGATGGTGCGGGTTTCGATTGGTGCGCTGCCGACCGAGCGGGGGGATGTGCAGCGGCCGTGGGCACGT‑CTGCAGGACGTGAt                                                                                                                                     >  2:698874/1‑150 (MQ=255)
              gggCCGAGCGGCTGAATGCATCCGGCGCTGCTTATGTGACGCCGGCTACACTGGACGGGCGGTGGATGGTGCGGGTTTCGATTGGTGCGCTGCCGACCGAGCGGGGGGATGTGCAGCGGCTGTGGGCACGT‑CTGCAGGACGTGATCAAgg                                                                                                                                >  1:1420775/1‑150 (MQ=255)
                  cGAGCGGCTGAATGCATCCGGCGCTGCTTATGTGACGCCGGCTACACTGGACGGGCGGTGGATGGTGCGGGTTTCGATTGGTGCGCTGCCGACCGAGCGGGGGGATGTGCAGCGGCTGTGGGCACGT‑CTGCAGGACGTGATCAAGGGCTg                                                                                                                            >  2:498077/1‑150 (MQ=255)
                      cGGCTGAATGCATCCGGCGCTGCTTATGTGACGCCGGCTACACTGGACGGGCGGTGGATGGTGCGGGTTTCGATTGGTGCGCTGCCGACCGAGCGGGGGGATGTGCAGCGGCTGTGGGCACGT‑CTGCAGGACGTGATCAAGGGCTGAAGc                                                                                                                        <  2:1261814/150‑1 (MQ=255)
                                 aTCCGGCGCTGCTTATGTGACGCCGGCTACACTGGACGGGCGGTGGATGGTGCGGGTTTCGATTGGTGCGCTGCCGACCGAGCGGGGGGATGTGCAGCGGCTGTGGGCACGT‑CTGCAGGACGTGATCAAggg                                                                                                                               >  2:129539/1‑132 (MQ=255)
                                 aTCCGGCGCTGCTTATGTGACGCCGGCTACACTGGACGGGCGGTGGATGGTGCGGGTTTCGATTGGTGCGCTGCCGACCGAGCGGGGGGATGTGCAGCGGCTGTGGGCACGT‑CTGCAGGACGTGATCAAggg                                                                                                                               <  1:129539/132‑1 (MQ=255)
                                       cgcTGCTGATGTGACCGCGGCTACACTGGACGGGCGGTTGATGGTGCGGGTTTCGATTGGTGCGCTGCCGCCCGAGCGGAGGGATGTGCAGCGGCTGTGGGCACGT‑CTGCAGGACGTGATCAAGCGCTGAAGCCTGTACGGTACCTGttt                                                                                                       <  1:620446/150‑3 (MQ=255)
                                        gctgctTATGTGACGCCGGCTACACTGGACGGGCGGTGGATGGTGCGGGTTTCGATTGGTGCGCTGCCGACCGAGCGGGGGGATGTGCAGCGGCTGTGGGCACGT‑CTGCAGGACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATg                                                                                                      >  2:753505/1‑150 (MQ=255)
                                             ttATGTGACGCCGGCTACACTGGACGGGCGGTGGATGGTGCGGGTTTCGATTGGTGCGCTGCCGACCGAGCGGGGGGATGTGCAGCGGCTGTGGGCACGT‑CTGCAGGACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCgg                                                                                                 <  2:1420775/150‑1 (MQ=255)
                                                           cTACACTGGACGGGCGGTGGATGGTGCGGGTTTCGATTGTTTCGCTGCCGACCGAGCGGGGGGATGTGCAGCGGCTGTGGGCACGT‑CTGCAGGACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCGGGCTTGCCCGCGAAg                                                                                   <  2:1045428/150‑1 (MQ=255)
                                                            tACACTGGACGGGCGGTGGATGGTGCGGGTTTCGATTGGTGCGCTGCCGACCGAGCGGGGGGATGTGCAGCGGCTGTGGGCACGT‑CTGCAGGACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCGGGCTTGCCCGCGAAgg                                                                                  <  1:236208/150‑1 (MQ=255)
                                                            tACACTGGACGGGCGGTGGATGGTGCGGGTTTCGATTGGTGCGCTGCCGACCGAGCGGGGGGATGTGCAGCGGCTGTGGGCACGT‑CTGCAGGACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCGGGCTTGCCCGCGAAgg                                                                                  <  1:963837/150‑1 (MQ=255)
                                                               acTGGACGGGCGGTGGATGGTGCGGGTTTCGATTGGTGCGCTGCCGACCGAGCGGGGGGATGTGCAGCGGCTGTGGGCACGT‑CTGCAGGACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCGGGCTTGCCCGCGAAGGGGc                                                                               >  2:158034/1‑150 (MQ=255)
                                                                   gACGGGCGGTGGATGGTGCGGGTTTCGATTGGTGCGCTGCCGACCGAGCGGGGGGATGTGCAGCGGCTGTGGGCACGT‑CTGCAGGACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCGGGCTTGCCCGCGAAGGGGCTGCa                                                                           >  1:1299823/1‑150 (MQ=255)
                                                                      gggCGGTGGATGGTGCGGGTTTCGATTGGTGCGCTGCCGACCGAGCGGGGGGATGTGCAGCGGCTGTGGGCACGT‑CTGCAGGACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCGGGCTTGCCCGCGAAGGGGCTGctttg                                                                        <  1:158034/150‑5 (MQ=255)
                                                                       ggCGGTGGATGGTGCGGGTTTCGATTGGTGCGCTGCCGACCGAGCGGGGGGATGTGCAGCGGCTGTGGGCACGT‑CTGCAGGACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCGGGCTTGCCCGCGAAggg                                                                                 >  2:18878/1‑140 (MQ=255)
                                                                       ggCGGTGGATGGTGCGGGTTTCGATTGGTGCGCTGCCGACCGAGCGGGGGGATGTGCAGCGGCTGTGGGCACGT‑CTGCAGGACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCGGGCTTGCCCGCGAAggg                                                                                 <  1:18878/140‑1 (MQ=255)
                                                                                tGGTGCGGGTTTCGATTGGTGCGCTGCCGACCGAGCGGGGGGATGTGCAGCGGCTGTGGGCACGT‑CTGCAGGACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCGGGCTTGCCCGCGAAGGGGCTGCTTTGCAGCCCCttc                                                              <  1:498077/150‑3 (MQ=255)
                                                                                   tGCGGGTTTCGATTGGTGCGCTGCCGACCGAGCGGGGGGATGTGCAGCGGCGGTGGGCACGT‑CTGCAGGACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCGGGCTTGCCCGCGAAGGGGCTGCAAAGCAGCCCCTGCAAt                                                           >  1:1265880/1‑150 (MQ=255)
                                                                                     cGGGTTTCGATTGGTGCGCTGCCGACCGAGCGGGGGGATGTGCAGCGGCTGTGGGCACGT‑CTGCAGGACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCGGGCTTGCCCGCGAAGGGGCTGCAAAGCAGCCCCTGCAATCt                                                         >  2:1345161/1‑150 (MQ=255)
                                                                                           tCGATTGGTGCGCTGCCGACCGAGCGGGGGGATGTGCAGCGGCTGTGGGCACGT‑CTGCAGGACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCGGGCTTGCCCGCGAAGGGGCTGCAAAGCAGCCCCTGCAATCTATCAGt                                                   <  2:1299823/150‑1 (MQ=255)
                                                                                              aTTGGTGCGCTGCCGACCGAGCGGGGGGATGTGCAGCGGCTGTGGGCACGT‑CTGCAGGACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCGGGCTTGCCCGCGAAGGGGCTGCAAAGCAGCCCCTGCaa                                                            <  2:758339/138‑1 (MQ=255)
                                                                                              aTTGGTGCGCTGCCGACCGAGCGGGGGGATGTGCAGCGGCTGTGGGCACGT‑CTGCAGGACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCGGGCTTGCCCGCGAAGGGGCTGCAAAGCAGCCCCTGCaa                                                            >  1:758339/1‑138 (MQ=255)
                                                                                                 ggTGCGCTGCCGACCGAGCGGGGGGATGTGCAGCGGCTGTGGGCACGT‑CTGCAGGACGTGATc                                                                                                                                    <  2:39046/63‑1 (MQ=255)
                                                                                                 ggTGCGCTGCCGACCGAGCGGGGGGATGTGCAGCGGCTGTGGGCACGT‑CTGCAGGACGTGATc                                                                                                                                    >  1:39046/1‑63 (MQ=255)
                                                                                                   tGCGCTGCCGACCGAGCGGGGGGATGTGCAGCGGCTGTGGGCACGT‑CTGCAGGACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCGGGCTTGCCCGCGAAGGGGCTGCAAagcag                                                                     >  2:86803/1‑124 (MQ=255)
                                                                                                   tGCGCTGCCGACCGAGCGGGGGGATGGGCAGCGGCTGTGGGCACGT‑CTGCAGGACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCGGGCTTGCCCGCGAAGGGGCTGCAAagcag                                                                     <  1:86803/124‑1 (MQ=255)
                                                                                                           cgaccgaGCGGGGGGATGTGCAGCGGCTGTGGGCACGT‑CTGCAGGACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCGGGCTTGCCCGCGACGGGGCTGGGAAGCAGCCCCTGCAATCTATCAGTGGCTCACCGCCTGaaa                                   <  2:15391/150‑1 (MQ=255)
                                                                                                            gaccgaGCGGGGGGATGTGCAGCGGCTGTGGGCACGT‑CTGCAGGACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCGGGCTTGCCCGCGAAGGGGCTGCTTTGCAGccc                                                                  >  1:646534/1‑118 (MQ=255)
                                                                                                            gaccgaGCGGGGGGATGTGCAGCGGCTGTGGGCACGT‑CTGCAGGACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCGGGCTTGCCCGCGAAGGGGCTGCAAAGCAGCCCCTGCAATCTATCAGTGGCTCACCGCCTGAAAg                                  >  2:511724/1‑150 (MQ=255)
                                                                                                            gaccgaGCGGGGGGATGTGCAGCGGCTGTGCGCAGGC‑CTGCAGGGCGCGGGTTATGTCTAAAGCCTGTAGGGGTGCGGTATGAGCGGGCTTGCCCGCGAAGGGGCGGCAAAGCAGCCCCTGCAATCTATCAGTGGCTCACCGCCTGAAAg                                  >  1:26594/1‑150 (MQ=255)
                                                                                                            gaccgaGCGGGGGGATGTGAAGCGGCTGTGGGCACGT‑CTGCAGGACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCGGGCTTGCCCGCGAAGGGGCTGCAAAGCAGCCAGTGCAATCTATCAGTAGCTCACCACCTGAAAg                                  >  1:164551/1‑150 (MQ=255)
                                                                                                               cgaGCGGGGGGATGTGCAGCGGCTGTGGGCACGT‑CTGCAGGACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCGGGCTTGCCCGCGAAGGGGCTGCAAAGCAGCCCCTGCAATCTATCAGTGGCTCACCGCCTGAAAGCTa                               >  1:283845/1‑150 (MQ=255)
                                                                                                                   cGGGGGGATGTGCAGCGGCTGTGGGCACGT‑CTGCAGGACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCGGGCTTGCCCGCGAAGGGGCTGCAAAGCAGCCCCTGCAATCTATCAGTGGCTCACCGCCTGAAAGCTACCtt                           >  2:570653/1‑150 (MQ=255)
                                                                                                                     gggggATGTGCAGCGGCTGTGGGCACGT‑CTGCAGGACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCGGGCTTGCCCGCGAAGGGGCTGCAAAGCAGCCCCTGCAATCTATCAGTGGCTCACCGCCTGAAAGCTACCTTTa                         >  2:1240350/1‑150 (MQ=255)
                                                                                                                               cAGCGGCTGTGGGCACGT‑CTGCAGGACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCGGGCTTGCCCGCGAAGGGGCTGCAAAGCAGCCCCTGCAATCTATCAGTGGCTCACCGCCTGAAAGCTAc                              <  2:1407441/135‑1 (MQ=255)
                                                                                                                               cAGCGGCTGTGGGCACGT‑CTGCAGGACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCGGGCTTGCCCGCGAAGGGGCTGCAAAGCAGCCCCTGCAATCTATCAGTGGCTCACCGCCTGAAAGCTAc                              >  1:1407441/1‑135 (MQ=255)
                                                                                                                               cAGCGGCTGTGGGCACGT‑CTGCAGGACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCGGGCTTGCCCGCGAAGGGGCTGCAAAGCAGCCCCTGCAATCTATCAGTGGCTCACCGCCTGAAAGCTACCTTTACGGCTGGCtt               >  1:735881/1‑150 (MQ=255)
                                                                                                                                aGCGGCTGTGGGCACGT‑CTGCAGGACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCGGGCTTGCCCGCGAAGGGGCTGCAAAGCAGCCCCTGCAATCTATCAGTGGCTCACCGCCTGAAAGCTACCTTTACGGCTGGCTTc              <  1:664518/150‑1 (MQ=255)
                                                                                                                                   ggCTGTGGGCACGT‑CTGCAGGACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCGGGCTTGCCCGCGAAGGGGCTGCAAAGCAGCCCCTGCAATCTATCAGTGGCTCACCGCCTGAAAGCTACCTTTACGGCTGGCTTCATa           <  1:698874/150‑1 (MQ=255)
                                                                                                                                   ggCTGTGGGCACGT‑CTGCAGGACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCGGGCTTGCCCGCGAAGGGGCTGCAAAGCAGCCCCTGCAATCTATCAGTGGCTCACCGCCTGAAAGCTACCTTTACGGCTGGCTTCATa           <  1:753505/150‑1 (MQ=255)
                                                                                                                                       ttgatcACGTCCTGCA‑GACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCGGGCTTGCCCGCGAAGGGGCTGCAAAGCAGCCCCTGCAATCTATCAGCGGCTCACCGCCTGAAAGCTACCTTTACGGCTGGCTTCATAGGCt       >  2:1320155/6‑150 (MQ=255)
                                                                                                                                         gggCACGT‑CTGCAGGACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCGGGCTTGCCCGCGAAGGGGCTGCAAAGCAGCCCCTGCAATCTATCAGTGGCTCACCGCCTGAAAGCTACCTTTACGGCTGGCTTCATAGGCTGc     <  2:283845/150‑1 (MQ=255)
                                                                                                                                           tcACGTCCTGCA‑GACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCGGGCTTGCCCGCGAAGGGGCTGCAAAGCAGCCCCTGCAATCTATCAGTGGCTCACCGCCTGAAAGCTACCTTTACGGCTGGCTTCATAGGc        >  1:1219624/2‑145 (MQ=255)
                                                                                                                                           tcACGTCCTGCA‑GACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCGGGCTTGCCCGCGAAGGGGCTGCAAAGCAGCCCCTGCAATCTATCAGTGGCTCACCGCCTGAAAGCTACCTTTACGGCTGGCTTCATAGGc        <  2:1219624/144‑1 (MQ=255)
                                                                                                                                           gCACGT‑CTGCAGGACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCGGGCTTGCCCGCGAAGGGGCTGCAAAGCAGCCCCTGCAATCTATCAGTGGCTCACCGCCt                                       <  1:873543/114‑1 (MQ=255)
                                                                                                                                           gCACGT‑CTGCAGGACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCGGGCTTGCCCGCGAAGGGGCTGCAAAGCAGCCCCTGCAATCTATCAGTGGCTCACCGCCt                                       >  2:873543/1‑114 (MQ=255)
                                                                                                                                           gCACGT‑CTGCAGGACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCGGGCTTGCCCGCGAAGGGGCTGCAAAGCAGCCCCTGCAATCTATCAGTGGCTCACCGCCTGAAAGCTACCTTTACGGCTGGCTTCATAGGCTGCCt   >  1:910832/1‑150 (MQ=255)
                                                                                                                                            cACGT‑CTGCAGGACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCGGGCTTGCCCGCGAAGGGGCTGCAAAGCAGCCCCTGCAATCTATCAGTGGCTCACCGCCTGAAAGCTACCTTTACGGCTGGCTTCATAGGCTGCCtt  <  2:735881/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GCATACCAAGGGCTGGGCCGAGCGGCTGAATGCATCCGGCGCTGCTTATGTGACGCCGGCTACACTGGACGGGCGGTGGATGGTGCGGGTTTCGATTGGTGCGCTGCCGACCGAGCGGGGGGATGTGCAGCGGCTGTGGGCACGT‑CTGCAGGACGTGATCAAGGGCTGAAGCCTGTACGGTACCTGTATGAGCGGGCTTGCCCGCGAAGGGGCTGCAAAGCAGCCCCTGCAATCTATCAGTGGCTCACCGCCTGAAAGCTACCTTTACGGCTGGCTTCATAGGCTGCCTT  >  NC_002947/2899063‑2899352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑