| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,032,688:1 | +G | 14.3% | coding (1952/2763 nt) | mgtA → | ATP‑dependent magnesium transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,032,688 | 1 | . | G | 14.3% | 126.3 / 20.5 | 42 | coding (1952/2763 nt) | mgtA | ATP‑dependent magnesium transporter |
| Reads supporting (aligned to +/- strand): ref base . (16/20); new base G (4/2); total (20/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.31e-01 | |||||||||||
GTGAAGTGGGCCTGGCGCCGGGCCAGCCCTTGCTCGGCCAGGACATCGAGGGCATGGACGACACCACCCTGAAGCTTCA‑GGTGGAGGAGCGCACCGTCTTCGCCAAGCTCACCCCGTTGCAGAAATCACGGGTGCTCAAGGCCCTGCA‑GGCCAATGGTCACACTGTGGGCTTCCTTGGCGACGGCATCAACGACGCCGCAGCCCTGCGCGATGCTGACGTCGGTATCTCGGTGGACAGCGGCACCGACATCGCCAAGGAATCGGCCGACATCATTTTGCTGGAAAAGA > NC_002947/3032541‑3032828 | gTGAAGTGGGCCTGGCGCCGGGCCAGCCCTTGCTCGGCCAGGACATCGAGGGCATGGACGACACCACCCTGAAGCTTCA‑GGTGGAGGAGCGCACCGTCTTCGCCAAGCTCACCCCGTTGCAGAAATCACGTGTGCTCAAGGCCCTGCA‑gg < 1:17974/150‑1 (MQ=255) tGAAGTGGGCCTGGCGCCGGGCCAGCCCTTGCTCGGCCAGGACATCGAGGGCATGGACGACACCGCCCTGAAGCTTCA‑GGTGGAGGAGCGCACCGTCTTCGCCAAGCTCACCCCGTTGCAGAAATCACGGGTGCTCAAGGCCCTGCA‑GGc < 2:1333072/150‑1 (MQ=255) aGTGGGCCTGGCGCCGGGCCAGCCCTTGCTCGGCCAGGACATCGAGGGCATGGACGACACCACCCTGAAGCTTCA‑GGTGGAGGAGCGCACCGTCTTCGCCAAGCTCACCCCGTTGCAGAAATCACGGGTGCTCAAGGCCCTGCA‑GGCCaa < 1:736091/150‑1 (MQ=255) ccGGGCCAGCCCTTGCTCGGCCAGGACATCGAGGGCATGGACGACACCACCCTGAAGCTTCA‑GGTGGAGGAGCGCACCGTCTTCGCCAAGCTCACCCCGTTGCAGAAATCACGGGTGCTCAAGGCCCTCCA‑GGCCAATGGTcacaatctg > 2:1170224/1‑145 (MQ=255) cGGGCCAGCCCTTGCTCGGCCAGGACATCGAGGGCATGGACGACACCACCCTGAAGCTTCA‑GGTGGAGGAGCGCACCGTCTTCGCCAAGCTCACCCCGTTGCAGAAATCACGGGTGCTCAAGGCCCTGCA‑GGCCAATGGTCACACTGTgg < 2:460423/150‑1 (MQ=255) gggCCAGCCCTTGCTCGGCCAGGACATCGAGGGCATGGACGACACCACCCTGAAGCTTCA‑GGTGGAGGAGCGCACCGTCTTCGCCAAGCTCACCCCGTTGCAGAAATCACGGGTGCTCAAGGCCCTGCA‑GGCCAATGGTCACACTGTggg > 1:140423/1‑150 (MQ=255) gCCCTTGCTCGGCCAGGACATCGAGGGCATGGACGACACCACCCTGAAGCTTCA‑GGTGGAGGAGCGCACCGTCTTCGCCAAGCTCACCCCGTTGCAGAAATCACGGGTGCTCAAGGCCCTGCA‑GGCCAATGGTCACACTGTGGGCTTCCt < 2:617936/150‑1 (MQ=255) cccTTGCTCGGCCAGGACATCGAGGGCATGGACGACACCACCCTGAAGCTTCA‑GGTGGAGGAGCGCACCGTCTTCGCCAAGCTCACCCCGTTGCAGAAATCACGGGTGCTCAAGGCCCTGGG‑GCCCAATGGTCACACTGTGGGCTTCCtt < 1:1106837/150‑1 (MQ=255) cccTTGCTCGGCCAGGACATCGAGGGCATGGACGACACCACCCTGAAGCTTCA‑GGTGGAGGAGCGCACCGTCTTCGCCAAGCTCACCCCGTTGCAGAAATCACGGGTGCTCAAGGCCCTGCA‑GGCCAATGGTCACACTGTGGGCTTCCtt < 2:551684/150‑1 (MQ=255) cGGCCAGGACATCGAGGGCATGGACGACACCACCCTGAAGCTTCA‑GGTGGAGGAGCGCACCGTCTTCGCCAAGCTCACCCCGTTGCAGAAATCACGGGTGCTCAAGGCCCTGCA‑GGCCAATGGTCACACTGTGGGCTTCCTTGGCGACgg < 1:1048656/150‑1 (MQ=255) gACATCGAGGGCATGGACGACACCACCCTGAAGCTTCA‑GGTGGAGGAGCGCACCGTCTTCGCCAAGCTCACCCCGTTGCAGAAATCACGGGTGCTCAAGGCCCTGCA‑GGGCAATGGTCACACTGTGGGCTTCCTTGGCGACGGCATCAac < 1:1359835/150‑1 (MQ=255) gACATCGAGGGCATGGACGACACCACCCTGAAGCTTCA‑GATGGAGGAGCGCACCGTCTTCGCCAAGCTCACCCCGTTGCAGAAATCACGGGTGCTCAAGGCCCTGCA‑GGCCAATGGTCACACTGTGGGCTTCCTTGGCGACGGCATCAac < 2:1140058/150‑1 (MQ=255) acgacACCACCCTGAAGCTTCA‑GGTGGAGGAGCGCACCGTCTTCGCCAAGCTCACCCCGTTGCAGAAATCACGGGTGCTCAAGGCCCTGCA‑GGCCAATGGTCACACTGTGGGCTTCCTTGGCGACGGCATCAac < 1:57028/134‑1 (MQ=255) acgacACCACCCTGAAGCTTCA‑GGTGGAGGAGCGCACCGTCTTCGCCAAGCTCACCCCGTTGCAGAAATCACGGGTGCTCAAGGCCCTGCA‑GGCCAATGGTCACACTGTGGGCTTCCTTGGCGACGGCATCAac > 2:57028/1‑134 (MQ=255) gacACCACCCTGAAGCTTCA‑GGTGGAGGAGCGCACCGTCTTCGCCAAGCTCACCCCGTTGCAGAAATCACGGGTGCTCAAGGCCCTGCA‑GGCCAATGGTCACACTGTgg < 1:1083808/109‑1 (MQ=255) gacACCACCCTGAAGCTTCA‑GGTGGAGGAGCGCACCGTCTTCGCCAAGCTCACCCCGTTGCAGAAATCACGGGTGCTCAAGGCCCTGCA‑GGCCAATGGTCACACTGTgg > 2:1083808/1‑109 (MQ=255) accaccCTGAAGCTTCA‑GGTGGAGGAGCGCACCGTCTTCGCCAAGCTCACCCCGTTGCAGAAATCACGGGTGCTCAAGGCCCTGCA‑GGCCAATGGTCACACTGTGGGCTTCCTTGGCGACGGCATCAACGACGCCGCAGCCCTGCGCGAt > 2:962925/1‑150 (MQ=255) accTGAAGCTTCAGGGTGGAGGAGCGCACCGTCTTCGCCAAGCTCACCCCGTTGCAGAAATCACGGGTGCTCAAGGCCCTGCA‑GGCCAATGGTCACACTGTGGGCTTCCTTTCCGACGGCATCAACGACGCCGCAGCCCTGCGCGATGCt > 1:74741/2‑150 (MQ=255) cTGAAGCTTCAGGGTGGAGGAGCGCACCGTCTTCGCCAAGCTCACCCCGTTGCAGAAATCACGGGTGCTCAAGGCCCTGCA‑GGCCAATGGTCACACTGTGGGCTTCCTTGGCGACGGCATCAACGACGCCGCAGCCCTGCGCGATGCTGa < 2:74741/150‑1 (MQ=255) cTTCA‑GGTGGAGGAGCGCACCGTCTTCGCCAAGCTCACCCCGTTGCAGAAATCACGGGTGCTCAAGGCCCTGCA‑GGCCAATGGTCACACTGTGGGCTTCCTTGGCGACGGCATCAACGACGCCGCAGCCCTGCGCGATGCTGACGTCGGt < 2:606468/150‑1 (MQ=255) tGGAGGAGCGCACCGTCTTCGCCAAGCTCACCCCGTTGCAGAAATCACGGGTGCTCAAGGCCCTGCA‑GGCCAATGGTCACACTGTGGGCTTCCTTGGCGACGGCATCAACGACGCCGCAGCCCTGCGCGATGCTGACGTCGGTATCTCgg > 1:67222/1‑150 (MQ=255) gcgcACCGTCTTCGCCAAGCTCACCCCGTTGCAGAAATCACGGGTGCTCAAGGCCCTGCA‑GGCCAATGGTCACACtgtg > 1:628944/1‑79 (MQ=255) gcgcACCGTCTTCGCCAAGCTCACCCCGTTGCAGAAATCACGGGTGCTCAAGGCCCTGCA‑GGCCAATGGTCACACtgtg < 2:628944/79‑1 (MQ=255) cGTCTTCGCCAAGCTCACCCCGTTGCAGAAATCACGGGTGCTCAAGGCCCTGCA‑GGCCAATGGTCACACTGTGGGCTTCCTTGGCGACGGCATCAACGACGCCGCAGCCCTGCGCGATGCTGACGTCGGTATCTCGGTGGACAGCGGCAc > 1:220469/1‑150 (MQ=255) tCTTCGCCAAGCTCACCCCGTTGCAGAAATCACGGGTGCTNAAGGCCCTGCA‑GGCCAATGGTCACACTGTGGGCTTCCTTGGCGACGGCATCAACGACGCCGCAGCCCTGCGCGATGCTGACGTCGGTATCTCGGTGGACAGCGGCACCg > 1:1307338/1‑150 (MQ=255) tCTTCGCCAAGCTCACCCCGTTGCAGAAATCACGGGTGCTCAAGGCCCTGCA‑GGCCAATGGTCACACTGTGGGCTTCCTTGGCGACGGCATCAACGACGCCGCAGCCCTGCGCGATGCTGACGTCGGTATCTCGGTGGACAGCGGCACCg > 2:1000134/1‑150 (MQ=255) aCCCCGTTGCAGAAATCACGGGTGCTCAAGGCCCTGCA‑GGCCAATGGTCACACTGTGGGCTTCCTTGGCGACGGCATCAACGACGCCGCAGCCCTGCGCGATGCTGACGTCGGTATCTCGGTGGACAGCGGCACCGACATCGCCAAGGaa > 1:973839/1‑150 (MQ=255) cccGTTGCAGAAATCACGGGTGCTCAAGGCCCTGCA‑GGCCAATGGTCACACTGTGGGCTTCCTTGGCGACGGCATCAACGACGCCGCAGCCCTGCGCGATGCTGACGTCGGTATCTCGGTGGACAGCGGCACCGACATCGCCAAGGAATc > 2:1354472/1‑150 (MQ=255) ccGTTGCAGAAATCACGGGTGCTCAAGGCCCTGCA‑GGCCAATGGTCACACTGTGGGCTTCCTTGGCGACGGCATCAACGACGCCGCAGCCCTGCGCGATGCTGACGTCGGTATCTCGGTGGACAGCGGCACCGACATCGCCAAGGAATc < 2:1057562/149‑1 (MQ=255) ccGTTGCAGAAATCACGGGTGCTCAAGGCCCTGCA‑GGCCAATGGTCACACTGTGGGCTTCCTTGGCGACGGCATCAACGACGCCGCAGCCCTGCGCGATGCTGACGTCGGTATCTCGGTGGACAGCGGCACCGACATCGCCAAGGAATc > 1:1057562/1‑149 (MQ=255) gTTGCAGAAATCACGGGTGCTCAAGGCCCTGCA‑GGCCAATGGTCACACTGTGGGCTTCCTTGGCGACGGCATCAACGACGCCGCAGCCCTGCGCGATGCTGACGTCGGTATCTCGGTGGACAGCGGCACCGACATCGCCAAGGAATCGGc < 1:50314/150‑1 (MQ=255) tCACGGGTGCTCAAGGCCCTGCA‑GGCCAATGGTCACACTGTGGGCTTCCTTGGCGACGGCATCAACGACGCCGCAGCCCTGCGCGATGCTGACGTCGGTATCTCGGTGGACAGCGGCACCGACATCGCCAAGGAATCGGCCGACATCAtt > 2:415438/1‑150 (MQ=255) ggTGCTCAAGGCCCTGCA‑GGCCAATGGTCACACTGTGGGCTTCCTTGGCGACGGCATCAACGACGCCGCAGCCCTGCGCGATGCTGACGTCGGTATCTCGGTGGACAGCGGCACCGACATCGCCAAGGAATCGGCCGACATCATTTTGCt < 1:962925/150‑1 (MQ=255) cTCCAGGCCCTGCC‑GGGCAATGGTCCCACTGTGGGCTTCCTTGGAGACGGCATCAACGACGCCGCAGCCCTGCGCGATGCTGACGTCGGTATCTCGGTGGACAGCGGCACCGACACCGCCAAGGAATCGGCCGACATCATTTTGCTGGaa < 2:422129/150‑1 (MQ=255) ccattggccTGCAGGGCCAATGGTCACACTGTGGGCTTCCTTGGCGACGGCATCAACGACGCCGCAGCCCTGCGCGATGCTGACGTCGGTATCTCGGTGGACAGCGGCACCGACATCGCCAAGGAATCGGCCGACATCATTTTGc > 1:603904/8‑145 (MQ=255) ccattggccTGCAGGGCCAATGGTCACACTGTGGGCTTCCTTGGCGACGGCATCAACGACGCCGCAGCCCTGCGCGATGCTGACGTCGGTATCTCGGTGGACAGCGGCACCGACATCGCCAAGGAATCGGCCGACATCATTTTGc < 2:603904/138‑1 (MQ=255) ccattggccTGCAGGGCCAATGGTCACACTGTGGGCTTCCTTGGCGACGGCATCAACGACGCCGCAGCCCTGCGCGATGCTGACGTCGGTATCTCGGTGGACAGCGGCACCGACATCGCCAAGGAATCGGCCGACATCATTTTGCTGGaa > 1:1099345/8‑150 (MQ=255) cattggCCCTGCA‑GGCCAATGGTCACACTGTGGGCTTCCTTGGCGACGGCATCAACGACGCCGCAGCCCTGCGCGATGCTGACGTCGGTATCTCGGTGGACAGCGGCACCGACATCGCCAAGGAATc < 1:942889/123‑1 (MQ=255) cattggCCCTGCA‑GGCCAATGGTCACACTGTGGGCTTCCTTGGCGACGGCATCAACGACGCCGCAGCCCTGCGCGATGCTGACGTCGGTATCTCGGTGGACAGCGGCACCGACATCGCCAAGGAATc > 2:942889/5‑127 (MQ=255) cattggccTGCAGGGCCAATGGTCACACTGTGGGCTTCCTTGGCGACGGCATCAACGACGCCGCAGCCCTGCGCGATGCTGACGTCGGTATCTCGGTGGACAGCGGCACCGACATCGCCAAGGAATCGGCCGACATCATTTTGCTGGaaa > 1:512208/7‑150 (MQ=255) cattggccTGCAGGGCCAATGGTCACACTGTGGGCTTCCTTGGCGACGGCATCAACGACGCCGCAGCCCTGCGCGATGCTGACGTCGGTATCTCGGTGGACAGCGGCACCGACATCGCCAAGGAATCGGCCGACATCATTTTGCTGGaaa > 1:1233169/7‑150 (MQ=255) cggCCCCGCA‑GGCGGATGGGCACACTGTGGGCTTCCTTGCCGACGGCATCAAGGACGACGCAGCGTTGCGCGATGCTGACGTCGGTATCTCGGTGGACAGCGGCACCGACATCGCCAAGGAATCGGCCGACATCATTTTGCTGGAAAaga < 2:186495/149‑1 (MQ=255) tggccTGCAGGGCCAATGGTCACACTGTGGGCTTCCTTGGCGACGGCATCAACGACGCCGCAGCCCTGCGCGATGCTGACGTCGGTATCTCGGTGGACAGCGGCACCGACATCGCCAAGGAATCGGCCGACATCATTTTGCTGGAAAaga < 1:988878/147‑1 (MQ=255) | GTGAAGTGGGCCTGGCGCCGGGCCAGCCCTTGCTCGGCCAGGACATCGAGGGCATGGACGACACCACCCTGAAGCTTCA‑GGTGGAGGAGCGCACCGTCTTCGCCAAGCTCACCCCGTTGCAGAAATCACGGGTGCTCAAGGCCCTGCA‑GGCCAATGGTCACACTGTGGGCTTCCTTGGCGACGGCATCAACGACGCCGCAGCCCTGCGCGATGCTGACGTCGGTATCTCGGTGGACAGCGGCACCGACATCGCCAAGGAATCGGCCGACATCATTTTGCTGGAAAAGA > NC_002947/3032541‑3032828 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |