| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,050,084:1 | +C | 7.8% | intergenic (+13/‑128) | PP_2662 → / → PP_2663 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,050,084 | 1 | . | C | 7.8% | 199.4 / 10.7 | 51 | intergenic (+13/‑128) | PP_2662/PP_2663 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (23/24); new base C (2/2); total (25/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01 | |||||||||||
TGGCGCTGTTCCATGACATCAACGACAACCTGAAACTGGTGGCCGAGTACAACCAGTTTTCCATCGACGGGCATGACACCAGTGCGCAGAACGAAGACACCGATACCTTTGCGGTGGGCGCGGTGTTGACCTGGTAAGCGGCCGGGGCCG‑CTAGGCGGCCCCGAATGACACGGTCTTTTACGCCCATGGAACCGCCCACCCGCTACCCAAGTAGCATTCGTCACCCACCACCACCTCCTTAAACTGATCACTCAGCCACCCCGTGCCGGAGACCACGATGCAGCAGGCTCATAACGACG > NC_002947/3049935‑3050233 | tgGCGCTGTTCCATGACATCAACGACAACCTGAAACTGGTGGCCGAGTACAACCAGTTTTCCATCGACGGGCATGACACCAGTGCGCAGAACGAAGACACCGATACCTTTGCGGTGGGCGCGGTGTTGACCTGGTAAGCGGCCGGGGCCg > 2:731007/1‑150 (MQ=255) tGTTCCATGACATCAACGACAACCTGAAACTGGTGGCCGAGTACAACCAGTTTTCCATCGACGGGCATGACACCAGTGCGCAGAACGAAGACACCGATACCTTTGCGGTGGGCGCGGTGTTGACCTGGTAAGCGGCCGGGGCCG‑CTAggc < 1:451519/150‑1 (MQ=255) tGTTCCATGACATCAACGACAACCTGAAACTGGTGGCCGAGTACAACCAGTTTTCCATCGACGGGCATGACACCAGTGCGCAGAACGAAGACACCGATACCTTTGCGGTGGGCGCGGTGTTGACCTGGTAAGCGGCCCCGGCCG‑Cttacc < 2:1162221/150‑5 (MQ=255) gTTCAATGACATCAACGACAACCTGAAACTGGTGGCCGAGTACAACCAGTTTTCCATCGACGGGCATGACACCAGTGCGCAGAACGAAGACACCGATACCTTTGCGGTGGGCGCGGTGTTGACCTGGTAAGCGGCCCCGGCCG‑Cttacca < 2:190488/150‑6 (MQ=255) aTGACATCAACGACAACCTGAAACTGGTGGCCGAGTACAACCAGTTTTCCATCGACGGGCATGACACCAGTGCGCAGAACGAAGACACCGATACCTTTGCGGTGGGCGCGGTGTTGACCTGGTAAGCGGCCGGGGCCGCCTA‑GCGGcccc < 1:232161/150‑1 (MQ=255) gACATCAACGACAACCTGAAACTGGTGGCCGAGTACAACCAGTTTTCCATCGACGGGCATGACACCAGTGCGCAGAACGAAGACACCGATACCTTTGCGGTGGGCGCGGTGTTGACCTGGTAAGCGGCCGGGGCCG‑CTAGGCGGCCCCGa > 1:1047917/1‑150 (MQ=255) ccTGAAACTGGTGGCCGAGTACAACCAGTTTTCCATCGACGGGCATGACACCAGTGCGCAGAACGAAGACACCGATACCTTTGCGGTGGGCGCGGTGTTGACCTGGTAAGCGGCCGGGGCCG‑CTAGGCGGCCCCGAATGACACGGTCttt < 1:938996/150‑1 (MQ=255) cTGAAACTGGTGGCCGAGTACAACCAGTTTTCCATCGACGGGCATGACACCAGTGCGCAGAACGAAGACACCGATACCTTTGCGGTGGGCGCGGTGTTGACCTGGTAAGCGGCCGGGGCCG‑CTAGGCGGCCCCGAATGACACGGTCtt > 2:358092/1‑148 (MQ=255) cTGAAACTGGTGGCCGAGTACAACCAGTTTTCCATCGACGGGCATGACACCAGTGCGCAGAACGAAGACACCGATACCTTTGCGGTGGGCGCGGTGTTGACCTGGTAAGCGGCCGGGGCCG‑CTAGGCGGCCCCGAATGACACGGTCtt < 1:358092/148‑1 (MQ=255) cTGAAACTGGTGGCCGAGTACAACCAGTTTTCCATCGACGGGCATGACACCAGTGCGCAGAACGAAGACACCGATAACTTTGCGGTGGGCGCGGTGTTGAAGTGGTAAGCGGACGGGGCCG‑GCATGCGGTGCCGAATAAAACGGGCtttt > 2:938996/1‑150 (MQ=255) aaCTGGTGGCCGAGTACAACCAGTTTTCCATCGACGGGCATGACACCAGTGCGCAGAACGAAGACACCGATACCTTTGCGGTGGGCGCGGTGTTGACCTGGTAAGCGGCCGGGGCCG‑CGAGGCGGCCCCGAATGACACGGTCTTTTACGc < 1:1011407/150‑1 (MQ=255) ggtggCCGAGTACAACCAGTTTTCCATCGACGGGCATGACACCAGTGCGCAGAACGAAGACACCGATACCTTTGCGGTGGGCGCGGTGTTGACCTGGTAAGCGGCCGGGGCCG‑CTAGGCGGCCCCGAATGACACGGTCTTTTACGCCCAt < 2:1333950/150‑1 (MQ=255) cGAGTACAACCAGTTTTCCATCGACGGGCATGACACCAGTGCGCAGAACGAAGACACCGATACCTTTGCGGTGGGCGCGGTGTTGACCTGGTAAGCGGCCGGGGCCG‑CTAGGCGGCCCCGAATGACACGGTCTTTTACGCCCATGGAAcc < 2:1047917/150‑1 (MQ=255) tACAACCAGTTTTCCATCGACGGGCATGACACCAGCGCGCAGAACGAAGACACCGATACCTTTGCGGTGGGCGCGGTGTTGACCTGGTAAGCGGCCGGGGCCG‑CTAGGCGGCCCCGAATGACACGGTCTTTTACGCCCATGGAACCGccc > 1:366503/1‑150 (MQ=255) ccAGTTTTCCATCGACGGGCATGACACCAGTGCGCAGAACGAAGACACCGATACCTTTGCGGTGGGCGCGGTGTTGACCTGGTAAGCGGCCGGGGCCG‑CTAGGCGGCCCCGAATGACACGGTCTTTTACGCCCATGGAACCGCCCACCCg > 1:829195/1‑150 (MQ=255) tCGACGGGCATGACACCAGTGCGCAGAACGAAGACACCGATACCTTTGCGGTGGGCGCGGTGTTGACCTGGTAAGCGGCCGGGGCCG‑CTAGGCGGCCCCGAATGACACGGTCTTTTACGCCCATGGAACCGCCCACCCGCTACCCAAGTa < 1:1133708/150‑1 (MQ=255) gCATGACACCAGTGCGCAGAACGAAGACACCGATACCTTTGCGGTGGGCGCGGTGTTGACCTGGTAAGCGGCCGGGGCCG‑CTAGGCGGCCCCGAATGACACGGTCTTTTACGCCCATGGaa < 2:18066/121‑1 (MQ=255) gCATGACACCAGTGCGCAGAACGAAGACACCGATACCTTTGCGGTGGGCGCGGTGTTGACCTGGTAAGCGGCCGGGGCCG‑CTAGGCGGCCCCGAATGACACGGTCTTTTACGCCCATGGaa > 1:18066/1‑121 (MQ=255) gCATGACACCAGTGCGCAGAACGAAGACACCGATACCTTTGCGGTGGGCGCGGTGTTGACCTGGTAAGCGGCCGGGGCCG‑CTAGGCGGCCCCGAATGACACGGTCTTTTACGCCCATGGAACCGCCCACCCGCTACCCAAGTAGCATTCg > 1:458634/1‑150 (MQ=255) tggcacCAGTGCGCAGAACGAAGACACCGATACCTTTGCGGTGGGCGCGGTGTTGACCTGGTAAGCGGCCGGGGCCG‑CTAGGCGGCCCCTAATGACACGGTCTTTTACGCCCATGGAACCGCCCACCCGCTACCCAAGTAGCATTCGTca < 2:442913/147‑1 (MQ=255) gACACCAGTGCGCAGAACGAAGACACCGATACCTTTGCGGTGGGCGCGGTGTTGACCTGGTAAGCGGCCGGGGCCG‑CTAGGCGGCCCCGAATGACACGGTCTTTTACGCCCATGGAACCGCCCACCCGCTACCCAAGTAGCATTCGt > 1:1078406/1‑147 (MQ=255) gACACCAGTGCGCAGAACGAAGACACCGATACCTTTGCGGTGGGCGCGGTGTTGACCTGGTAAGCGGCCGGGGCCG‑CTAGGCGGCCCCGAATGACACGGTCTTTTACGCCCATGGAACCGCCCACCCGCTACCCAAGTAGCATTCGt < 2:1078406/147‑1 (MQ=255) acacCAGTGCGCAGAACGAAGACACCGATACCTTTGCGGTGGGCGCGGTGTTGACCTGGTAAGCGGCCGGGGCCG‑CTAGGCGGCCCCGa > 1:1275740/1‑89 (MQ=255) acacCAGTGCGCAGAACGAAGACACCGATACCTTTGCGGTGGGCGCGGTGTTGACCTGGTAAGCGGCCGGGGCCG‑CTAGGCGGCCCCGa < 2:1275740/89‑1 (MQ=255) acCAGTGCGCAGAACGAAGACACCGATACCTTTGCGGTGGGCGCGGTGTTGACCTGGTAAGCGGCCGGGGCCG‑CTAGGCGGCCCCGAATGACACGGTCTTTTACGCCCATGGAACCGCCCAccc > 2:197035/1‑124 (MQ=255) acCAGTGCGCAGAACGAAGACACCGATACCTTTGCGGTGGGCGCGGTGTTGACCTGGTAAGCGGCCGGGGCCG‑CTAGGCGGCCCCGAATGACACGGTCTTTTACGCCCATGGAACCGCCCAccc < 1:197035/124‑1 (MQ=255) gAACGAAGACACCGATACCTTTGCGGTGGGCGCGGTGTTGACCTGGTAAGCGGCCGGGGCCG‑CTAGGCGTCCCCGAATGACACGGTCTTTTACGCCCATGGAACCGCCCACCCGCTACCCAAGTAGCATTCGTCACCCACCACCAcctcc < 2:976662/150‑1 (MQ=255) aGACACCGATACCTTTGCGGTGGGCGCGGTGTTGACCTGGTAAGCGGCCGGGGCCG‑CTAGGCGGCCCCGAATGACACGGTCTTTTACGCCCATGGAACCGCCCACCCGCTACCCAAGTAGCATTCGTCACCCACCACCACCTCCTTAAAc > 2:1313786/1‑150 (MQ=255) cacCGATACCTTTGCGGTGGGCGCGGTGTTGACCTGGTAAGCGGCCGGGGCCG‑CTAGGCGGCCCCGAATGACACGGTCTTTTACGCCCATGGAACCGCCCACCCGCTACCCAAGTAGCATTCGTCACCCACCACCACCTCCTTAAACTGa > 2:280914/1‑150 (MQ=255) acCGATACCTTTGCGGTGGGCGCGGTGTTGACCTGGTAAGCGGCCGGGGCCG‑CTAGGCGGCACCGAATGACACGGCCACATCCCATAAGTTGATCTCCGACCCGCTACCCAAGTAGCATTCGTCACCCACCACCACATCCTTAAACTGAt > 1:324311/1‑150 (MQ=255) cTTTGCGGTGGGCGCGGTGTTGACCTGGTAAGCGGCCGGGGCCG‑CTAGGCGGCCCCGAATGACACGGTCTTTTACGCCCATGGAACCGCCCACCCGCTACCCAAGTAGCATTCGTcac > 2:558730/1‑118 (MQ=255) cTTTGCGGTGGGCGCGGTGTTGACCTGGTAAGCGGCCGGGGCCG‑CTAGGCGGCCCCGAATGACACGGTCTTTTACGCCCATGGAACCGCCCACCCGCTACCCAAGTAGCATTCGTcac < 1:558730/118‑1 (MQ=255) ttGCGGTGGGCGCGGTGTTGACCTGGTAAGCGGCCGGGGCCG‑CTAGGCGGCCCCGAATGACACGGTCTTTTACGCCCATGGAACCGCCCACCCGCTACCCAAGTAGCATTCGTCACCCACCACCACCTCCTTAAACTGATCACTCAGCCa < 1:1313786/150‑1 (MQ=255) gcgcGGTGTTGACCTGGTAAGCGGCCGGGGCCG‑CTAGGCGGCCCCGAATGACACGGTCtttt > 2:1307115/1‑62 (MQ=255) gcgcGGTGTTGACCTGGTAAGCGGCCGGGGCCG‑CTAGGCGGCCCCGAATGACACGGTCtttt < 1:1307115/62‑1 (MQ=255) cTGGTAAGCGGCCGGGGCCG‑CTAGGCGGCCCCGAATGACACGGTCTTTTACGCCCATGGAACCGCCCACCCGCTACCCAAGTAGCATTCGTCACCCACCACCACCTCCTTAAACTGATCACTCAGCCACCCCGTGCCGGAGACCACGATg > 2:557916/1‑150 (MQ=255) cTGGTAAGCGGCCGGGGCCG‑CTAGGCGGCCCCGAATGACACGGTCTTTTACGCCCATGGAACCGCCCACCCGCTACCCAAGTAGCATTCGTCACCCACCACCACCTCCTTAAACTGATCACTCAGCCACCCCGTGCCGGAGACCACGATg > 1:427375/1‑150 (MQ=255) tGGTAAGCGGCCGGGGCCG‑CTAGGCGGCCCCGAATGACACGGTCTTTTACGCCCATGGAACCGCCCACCCGCTACCCAAGTAGCATTCGTCACCCACCACCACCTCCTTAAACTGATCACTCAGCCACCCCGTGCCGGAGACCACGATgc > 2:962358/1‑150 (MQ=255) ggTAAGCGGCCGGGGCCG‑CTAGGCGGCCCCGAATGACACGGTCTTTTACGCCCATGGAACCGCCCACCCGCTACCCAAGTAGCATTCGTCACCCACCACCACCTCCTTAAACTGATCACTCAGCCACCCCGTGCCGGAGACCACGATgca < 2:427375/150‑1 (MQ=255) gTAAGCGGCCGGGGCCG‑CTAGGCGGCCCCGAATGACACGGTCTTTTACGCCCATGGAACCGCCCACCCGCTACCCAAGTAGCATTCGTCACCCACCACCACCTCCTTAAACTGATCACTCAGCCACCCCGTGCCGGAGACCACGATgcag > 1:272017/1‑150 (MQ=255) aaGCGGCCGGGGCCG‑CTAGGCGGCCCCGAATGACACGGTCTTTTACGCCCATGGAACCGCCCACCCGCTACCCAAGTAGCATTCGTCACCCACCACCACCTCCTTAAACTGATCACTCAGCCACCCCGTGCCGGAGACCACGATgcagca < 2:829195/150‑1 (MQ=255) gCGGCCGGGGCCG‑CTAGGCGGCCCCGAATGACACGGTCTTTTACGCCCATGGAACCGCCCACCCGCTACCCAAGTAGCATTCGTCACCCACCACCACCTCCTTAAACTGATCACTCAGCCACCCCGTGCCGGAGACCACGATGCAGCAgg < 2:324311/150‑1 (MQ=255) ggCCGGGGCCG‑CTAGGCGGCCCCGAATGACACGGTCTTTTACGCCCATGGAACCGCCCACCCGCTACCCAAGTAGCATTCGTCACCCACCACCACCTCCTTAAACTGATCACTCAGCCACCCCGTGCCGga < 1:787818/131‑1 (MQ=255) ggCCGGGGCCG‑CTAGGCGGCCCCGAATGACACGGTCTTTTACGCCCATGGAACCGCCCACCCGCTACCCAAGTAGCATTCGTCACCCACCACCACCTCCTTAAACTGATCACTCAGCCACCCCGTGCCGga > 2:787818/1‑131 (MQ=255) attcGGGGCCGCCTA‑GCGGCCCCGAATGACACGGTCTTTTACGCCCATGGAACCGCCCACCCGCTACCCAAGTAGCATTCGTCACCCACCACCACCTCCTTAAACTGATCACTCAGCCACCCCGTGCCGGAGACCACGATGCAGCAGGCt > 1:64442/4‑150 (MQ=255) gCCGGGGCCG‑CTAGGCGGCCCCGAATGACACGGTCTTTTACGCCCATGGAACCGCCCACCCGCTACCCAAGTAGCATTCGTCACCCACCACCACCTCCTTAAACTGATCACTCAGCCACCCCGTGCCGGAGACCACGATGCAGCAGGCTc < 1:280914/150‑1 (MQ=255) tcGGGGCCGCCTA‑GCGGCCCCGAATGACACGGTCTTTTACGCCCATGGAACCGCCCACCCGCTACCCAAGTa < 2:95136/71‑1 (MQ=38) tcGGGGCCGCCTA‑GCGGCCCCGAATGACACGGTCTTTTACGCCCATGGAACCGCCCACCCGCTACCCAAGTa > 1:95136/2‑72 (MQ=38) ggCCG‑CTAGGCGGCCCCGAATGACACGGTCTTTTACGCCCATGGAACCGCCCACCCGCTACCCAAGTAGCATTCGTCACCCACCACCACCTCCTTAAACTGATCACTc > 1:1057421/1‑108 (MQ=255) ggCCG‑CTAGGCGGCCCCGAATGACACGGTCTTTTACGCCCATGGAACCGCCCACCCGCTACCCAAGTAGCATTCGTCACCCACCACCACCTCCTTAAACTGATCACTc < 2:1057421/108‑1 (MQ=255) gCCG‑CTAGGCGGCCCCGAATGACACGGTCTTTTACGCCCATGGAACCGCCCACCCGCTACCCAAGTAGCATTCGTCACCCACCACCACCTCCTTAAACTGATCACTCAGCCACCCCGTGCCGGAGACCACGATGCAGCAGGCTCATAacg > 1:735839/1‑150 (MQ=255) ccG‑CTAGGCGGCCCCGAATGACACGGTCTTTTACGCCCATGGAACCGCCCACCCGCTACCCAAGTAGCATTCGTCACCCACCACCACCTCCTTAAACTGATCACTCAGCCACCCCGTGCCGGAGACCACGATGCAGCAGGCTCATAacga > 1:156933/1‑150 (MQ=255) g‑CTAGGCGGCCCCGAATGACACGGTCTTTTACGCCCATGGAACCGCCCACCCGCTACCCAAGTAGCATTCGTCACCCACCACCACCTCCTTAAACTGAt > 2:377113/1‑99 (MQ=255) g‑CTAGGCGGCCCCGAATGACACGGTCTTTTACGCCCATGGAACCGCCCACCCGCTACCCAAGTAGCATTCGTCACCCACCACCACCTCCTTAAACTGAt < 1:377113/99‑1 (MQ=255) g‑CTAGGCGGCCCCGAATGACACGGTCTTTTACGCCCATGGAACCGCCCACCCGCTACCCAAGTAGCATTCGTCACCCACCACCACCTCCTTAAACTGATCACTCAGCCACCCCGTGCCGGAGACCACGATGCAGCAGGCTCATAacgacg < 1:731007/150‑1 (MQ=255) | TGGCGCTGTTCCATGACATCAACGACAACCTGAAACTGGTGGCCGAGTACAACCAGTTTTCCATCGACGGGCATGACACCAGTGCGCAGAACGAAGACACCGATACCTTTGCGGTGGGCGCGGTGTTGACCTGGTAAGCGGCCGGGGCCG‑CTAGGCGGCCCCGAATGACACGGTCTTTTACGCCCATGGAACCGCCCACCCGCTACCCAAGTAGCATTCGTCACCCACCACCACCTCCTTAAACTGATCACTCAGCCACCCCGTGCCGGAGACCACGATGCAGCAGGCTCATAACGACG > NC_002947/3049935‑3050233 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |