| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,100,605:1 | +C | 8.2% | coding (1341/1470 nt) | PP_2709 → | long‑chain fatty acid‑‑CoA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,100,605 | 1 | . | C | 8.2% | 186.4 / 11.0 | 49 | coding (1341/1470 nt) | PP_2709 | long‑chain fatty acid‑‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base . (24/21); new base C (2/2); total (26/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.45e-01 | |||||||||||
CAAAGTGGTGTGATCGCCCAGGCTTTCGTGCATGGCGAAGCCTTGCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGTTGTGAGGTTCACAATGTCCTTTTT > NC_002947/3100456‑3100753 | cAAAGTGGTGTGATCGCCCAGGCTTTCGTGCATGGCGAAGCCTTGCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATTCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAgg > 2:274886/1‑150 (MQ=255) aaaGTGGTGTGATCGCCCAGGCTTTCGTGCATGGCGAAGCCTTGCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAggc > 2:340822/1‑149 (MQ=255) tggtgTGATCGCCCAGGCTTTCGTGCATGGCGAAGCCTTGCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAggcgtgc < 1:340822/150‑6 (MQ=255) cccAGGCTTTCGTGCATGGCGAAGCCTTGCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGGCGTGCAccctg < 1:558191/145‑5 (MQ=255) cccAGGCTTTCGTGCATGGCGAAGCCTTGCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGGCGTGCAccctg > 2:558191/1‑141 (MQ=255) cTTTCGTGCATGGCGAAGCCTTGCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCACGCCTGGCGCCGCTTGc < 2:117408/150‑1 (MQ=255) tGCATGGCGAAGCCTTGCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCACGc < 1:1283489/130‑1 (MQ=255) tGCATGGCGAAGCCTTGCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCACGc > 2:1283489/1‑130 (MQ=255) cTTGCCACGCAACCTTGCCTTGCTCTGTCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCt > 2:451903/1‑150 (MQ=255) ttGCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCtt > 1:822841/1‑150 (MQ=255) gCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGa < 1:779408/150‑1 (MQ=255) ccACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTa < 1:1151304/139‑1 (MQ=255) ccACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTa > 2:1151304/1‑139 (MQ=255) aCGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGGACGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGa > 1:545076/1‑150 (MQ=255) aaCCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAAcc > 2:321271/1‑150 (MQ=255) aCCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAAccc > 2:232955/1‑150 (MQ=255) ttgccttgcTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGa < 2:533734/150‑1 (MQ=255) ttgccttgcTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGa < 1:232955/150‑1 (MQ=255) cttgcTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGc < 2:833/150‑1 (MQ=255) ggCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCACGCCTGGCGCCGCTTGCCCGTGCCGCTTAGCACGCTTGACGAAACCCTGACTGCCAATGgcc < 2:1033678/150‑1 (MQ=255) ccGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTa < 1:61374/115‑1 (MQ=255) ccGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTa > 2:61374/1‑115 (MQ=255) cGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTg > 1:705863/1‑107 (MQ=255) cGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTg < 2:705863/107‑1 (MQ=255) tGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGccgcccgc < 1:102605/150‑1 (MQ=255) cAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGAt > 1:294049/1‑150 (MQ=255) cATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGc < 2:42344/92‑1 (MQ=255) cATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGc > 1:42344/1‑92 (MQ=255) tCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGc > 2:1324635/1‑144 (MQ=255) tCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGc < 1:1324635/144‑1 (MQ=255) gCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCGAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCTGTTAGCACGCTTGACGAAACGCTGAGTACGAATGGCCGCCCGCGACGTGAGGCGATGTTGCAGCGGGACCACACCCtg > 1:67421/1‑150 (MQ=255) aGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCg > 2:1153299/1‑150 (MQ=255) aGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCg > 1:845495/1‑150 (MQ=255) cAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGTGGCGATCTTGCAGCGTTACCATACCCTGTTGTCCGAc < 1:274886/150‑1 (MQ=255) cAACGCGCGATTGCCCGATTACGCCAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACAtctc > 2:153450/1‑150 (MQ=255) gcgcgATTGCCCGATTACGCCAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGTtg > 1:190174/1‑150 (MQ=255) cgATTGCCCGATTACGCCAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGTTGTGa < 1:451903/150‑1 (MQ=255) gATTGCCCGATTACGCCAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCCGCGTTACCACACCCTGTTGTCCGACATCTCGTTGTGAg < 2:822841/150‑1 (MQ=255) aTTGCCCGATTACGCCAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGTTGTGAgg > 1:786922/1‑150 (MQ=255) ttGCCCGATTACGCCAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGTTGTGAGGt < 2:190174/150‑1 (MQ=255) cccGATTACGCCAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGTTGTGAGGTTca > 2:38441/1‑150 (MQ=255) ccGATTACGCCAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGTTGTGAGGTTcac > 2:1411955/1‑150 (MQ=255) ccGATTACGCCAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGTTGTGAGGTTcac > 2:1092671/1‑150 (MQ=255) ccGATTACGCCAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGTTGTGAGGTTcac > 1:1054119/1‑150 (MQ=255) ccGATTACGCCAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTAGTCCGACATCTCGTTGTGAGGTTcac > 1:361143/1‑150 (MQ=255) gATTCCGCCAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCCCTTAGCACGCTTTACGAACCCCTGACTGCCAATGGCCGCCCGCGACGTCAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGTTGTGAGGTTCACaa < 2:545076/150‑1 (MQ=255) ggcGCCAGGCGTGCAC‑CCTGGCGTCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCa < 1:1082774/99‑1 (MQ=255) ggcGCCAGGCGTGCAC‑CCTGGCGTCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCa > 2:1082774/3‑101 (MQ=255) aCGCCAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGTTGTGAGGTTCACAATGTc < 1:38441/150‑1 (MQ=255) ccAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGTTGTGAGGTTCACAATGTCCtt < 1:1092671/150‑1 (MQ=255) cAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGTTGTGAGGTTcac > 2:432737/1‑140 (MQ=255) cAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGTTGTGAGGTTcac < 1:432737/140‑1 (MQ=255) gg‑gTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGTTGTGAGGTTCACAATGTCCttttt < 1:153450/150‑1 (MQ=255) gg‑gTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGTTGTGAGGTTCACAATGTCCttttt < 2:361143/150‑1 (MQ=255) | CAAAGTGGTGTGATCGCCCAGGCTTTCGTGCATGGCGAAGCCTTGCCACGCAACCTTGCCTTGCTCTGGCCGCTGGATCCCGGCATCAGCGACGAGGCCATCGAACAGGCCGTACAGCAATGCAACGCGCGATTGCCCGATTACGCCAGG‑GTGCACGCCTGGCGCCGCTTGCCAGTGCCTCTTAGCACGCTTGACGAAACCCTGACTGCCAATGGCCGCCCGCGACGTGAGGCGATCTTGCAGCGTTACCACACCCTGTTGTCCGACATCTCGTTGTGAGGTTCACAATGTCCTTTTT > NC_002947/3100456‑3100753 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |