| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,423,575 | C→G | 6.5% | P564A (CCA→GCA) | PP_3038 → | TraC domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,423,575 | 0 | C | G | 6.5% | 189.9 / 4.7 | 62 | P564A (CCA→GCA) | PP_3038 | TraC domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base C (28/29); new base G (2/2); total (31/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.96e-01 | |||||||||||
GCGTTCCAAGCCATCCGAGGTAAGTGGATCGTCGAGCTGGGCGAATTGGACAGCTTCAACAAGGCAGAGAGCACCAAGGCCAAGCAATTCTTCTCGGCA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCGGCGTTATTGGCCGGTCGCTTGCGTCAAGGTGGACCTTGAAGCATTGCGTCGCGCTCGTGACCAGCTGTGGGCTGAG > NC_002947/3423428‑3423718 | gcgTTCCAAGCCATCCGAGGTAAGTGGATCGTCGAGCTGGGCGAATTGGACAGCTTCAACAAGGCAGAGAGCACCAAGGCCAAGCAATTCTTCTCGGCA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCa > 1:1420396/1‑150 (MQ=255)gcgTTCCAAGCCATCCGAGGTAAGTGGATCGTCGAGCTGGGCGAATTGGACAGCTTCAACAAGGCAGAGAGCACCAAGGCCAAGCAATTCTTCTCGGCA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGACa > 1:139801/1‑150 (MQ=255) ttCCAAGCCATCCGAGGTAAGTGGATCGTCGAGCTGGGCGAATTGGACAGCTTCAACAAGGCAGAGAGCACCAAGGCCAAGCAATTCTTCTCGGCA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGt > 1:1191403/1‑150 (MQ=255) aaGCCATCCGAGGTAAGTGGATCGTCGAGCTGGGCGAATTGGACAGCTTCAACAAGGCAGAGAGCACCCAGCTCGACCAATTCTTCTCGGTA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAgt < 1:174023/150‑1 (MQ=255) aaGCCATCCGAGGTAAGTGGATCGTCGAGCTGGGCGAATTGGACAGCTTCAACAAGGCAGAGAGCACCAAGGCCAAGCAATTCTTCTCGGCA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAgt < 1:221293/150‑1 (MQ=255) cATCCGAGGTAAGTGGATCGTCGCGCTGGGCGGGTTGTACAGCTGCAACAAGGCAGCGCGCACAAAGGACAAGC‑ACAATCCTCGCCAGTGAACCCGGACGTTCCGCGAAAGCTATGGGCGAAGAACGAGAGAAATACCACGTCAgtgtgt > 1:1033938/1‑150 (MQ=255) tCCGAGGTAAGTGGATCGTCGAGCTGGGCGAATTGGACAGCTTCAACAAGGCAGAGAGCACCAAGGCCAAGCAATTCTTCTCGGCA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGttt > 2:1383698/1‑150 (MQ=255) gAGGTAAGTGGATCGTCGAGCTGGGCGAATTGGACAGCTTCAACAAGGCAGAGAGCACCAAGGCCAAGCAATTCTTCTCGGCA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTcg > 2:1145654/1‑150 (MQ=255) aGGTAAGTGGATCGTCGAGCTGGGCGAATTGGACAGCTTCAACAAGGCAGAGAGCACCAAGGCCAAGCAATTCTTCTCGGCA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTcgc < 1:368552/150‑1 (MQ=255) gtgtATCGTCGAGCGGGGCGAATTGGACAGCTTCAGCAAGGGAGAGAGCACGAAGGCCAAGCAATTCTTCTCGGGA‑TCTATCGACACTTACCGCGAAAGCTATAGGCGAAGAACGAGTGCAGTGCCACGTCAGTGTGTTTTCGCGGGTac > 1:575584/1‑150 (MQ=255) gTGGATCGTCGAGCTGGGCGAATTGGACAGCTTCAACAAGGCAGAGAGCACCAAGGCCAAGCAATTCTTCTCGGCA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTac < 2:1420396/150‑1 (MQ=255) tGGATCGTCGAGCTGGGCGAATTGGACAGCTTCAACAAGGCAGAGAGCACCAAGGCCAAGCAATTCTTCTCGGCA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTacc > 2:298302/1‑150 (MQ=255) ggATCGTCGAGCTGGGCGAATTGGACAGCTTCAACAAGGCAGAGAGCACCAAGGCCAAGCAATTCTTCTCGGCA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTacca < 2:1132101/150‑1 (MQ=255) gggCGAATTGGACAGCTTCAACAAGGCAGAGAGCACCAAGGCCAAGCAATTCTTCTCGGCA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAgtgt < 2:1152176/121‑1 (MQ=255) gggCGAATTGGACAGCTTCAACAAGGCAGAGAGCACCAAGGCCAAGCAATTCTTCTCGGCA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAgtgt > 1:1152176/1‑121 (MQ=255) ggCGAAGTGGACAGCTTCAACAAGTCAGAGAGCACCAAGGCCAAGCAATTCTTCTCGGCA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGaa < 1:649748/150‑1 (MQ=255) gACAGCTTCAACAAGGCAGAGAGCACCAAGGCCAAGCAATTCTTCTCGGCA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAg < 2:1191403/150‑1 (MQ=255) gACAGCTTCAACAAGGCAGAGAGCACCAAGGCCAAGCAATTCTTCTCGGCA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAg < 1:921353/150‑1 (MQ=255) aCAGCTTCAACAAGGCAGAGAGCACCAAGGCCAAGCAATTCTTCTCGGCA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAgg > 1:1036554/1‑150 (MQ=255) cAGCTTCAACAAGGCAGAGAGCACCAAGGCCAAGCAATTCTTCTCGGCA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGa > 1:256081/1‑150 (MQ=255) cAGCTTCAACAAGGCAGAGAGCACCAAGGCCAAGCAATTCTTCTCGGCA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGa > 1:178656/1‑150 (MQ=255) aGCTTCAACAAGGCAGAGAGCACCAAGGCCAAGCAATTCTTCTCGGCA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGAc > 2:783568/1‑150 (MQ=255) ttCAACAAGGCAGAGAGCACCAAGGCCAAGCGATTCTTCTCGGCA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACAACCAACCAGGATGAATACCTTAAGGACGcc > 2:876190/1‑150 (MQ=255) ttCAACAAGGCAGAGAGCACCAAGGCCAAGCAATTCTTCTCGGCA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTa < 1:354055/118‑1 (MQ=255) ttCAACAAGGCAGAGAGCACCAAGGCCAAGCAATTCTTCTCGGCA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTa > 2:354055/1‑118 (MQ=255) ttCAACAAGGCAGAGAGCACCAAGGCCAAGCAATTCTTCTCGGCA‑TCTATCAACATTTATCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGcc > 1:175529/1‑150 (MQ=255) acaaGGCAGAGAGCACCAAGGCCAAGCAATTCTTCTCGGCA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCg < 1:1383698/150‑1 (MQ=255) aGGCAGAGAGCACCAAGGCCAAGCAATTCTTCTCGGCA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATa > 1:60205/1‑130 (MQ=255) aGGCAGAGAGCACCAAGGCCAAGCAATTCTTCTCGGCA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATa < 2:60205/130‑1 (MQ=255) gCAGAGAGCACCAAGGCCAAGCAATTCTTCTCGGCA‑TCTATCCACACTTAAACCGGATCAAAGGGGGGGAGGACGAGAGACGGGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGAGGAATACCTTAAGGACGCCACCGGTAAc > 1:1243266/1‑150 (MQ=255) aCCAAGGCCAAGCAATTCTTCTCGGCA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCGGCGTTAt < 2:789738/150‑1 (MQ=255) cAAGGCCAAGCAATTCTTCTCGGCA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTaa > 1:1127253/1‑123 (MQ=255) cAAGGCCAAGCAATTCTTCTCGGCA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTaa < 2:1127253/123‑1 (MQ=255) cAAGGCCAAGCAATTCTTCTCGGCA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGGGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCGGCGTTATTg < 1:384716/150‑1 (MQ=255) aGGCCAAGCAATTCTTCTCGGCA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACAACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCGGCGTTATTGGc < 1:876190/150‑1 (MQ=255) gCCAAGCAATTCTTCTCGGCA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCGGCGTTATTGGCCg < 2:1243266/150‑1 (MQ=255) gCCAAGCAATTCTTCTCGGCA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCGGCGTTATTGGCCg > 2:601039/1‑150 (MQ=255) ccAAGCAATTCTTCTCGGCA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCGGCGTTATTGGCCgg > 2:1190381/1‑150 (MQ=255) aGCAATTCTTCTCGGCA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCGGCGTTATTGGCCGGTCg > 2:884499/1‑150 (MQ=255) aaTTCTTCTCGGCA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCGGCGTTATTGGCCGGTCGCtt < 1:1145654/150‑1 (MQ=255) ctcGGCA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCGGCGTTATTGGCCGGTCGCTTGCGTCaa < 2:1036554/150‑1 (MQ=255) tcGGCA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCGGCGTTATTGGCCGGTCGCTTGCGTCAAg < 1:783568/150‑1 (MQ=255) tCTAGCGACTCTGACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTGGCAGGTACCACCAACCGGGATGAATACCTTAAGGACGCCACAGGGAACCTGCGTTATTGGCCGGTCGCTTGCGTCAAGGTGGAc < 2:575584/150‑1 (MQ=255) tCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGAc < 2:744422/98‑1 (MQ=255) tCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGAc > 1:744422/1‑98 (MQ=255) gACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCGGCGTTATTGGCCGGTCGCTTGCGTCAAGGTGGACCTTGaa < 2:178656/150‑1 (MQ=255) acacTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCGGCGTTATTGGCCGGTCGCTTGCGTCAAGGTGGACCTTGAAg < 1:298302/150‑1 (MQ=255) cTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCGGCGTTATTGGCCGGTCGCTTGCGTCAAGGTGGACCTTGAAGCAt < 2:1033938/150‑1 (MQ=255) ttACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAg > 2:210068/1‑88 (MQ=255) ttACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAg < 1:210068/88‑1 (MQ=255) ttACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCGGCGTTATTGGCCGGTCGCTTGCGTCAAGGTGGACCTTGAAGCAtt > 1:792648/1‑150 (MQ=255) aaGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCGGCGTTATTGGCCGGTCGCTTGCGTCAAGGTGGACCTTGAAGCATTGCGTCgcgc > 2:572454/1‑150 (MQ=255) ggCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCGGCGTTATTGGCCGGTCGCTTGCGTCAAGGTGGACCTTGAAGCATTGCGTCGCGCTCGTGAcc > 2:695480/1‑150 (MQ=255) agaaCGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCAc > 2:1216851/1‑74 (MQ=255) agaaCGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCAc < 1:1216851/74‑1 (MQ=255) gaaCGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCGGCGTTATTGGCCGGTCGCTTGCGTCAAGGTGGACCTTGAAGCATTGCGTCGCGCTCGTGACCAGCtgt < 1:1128686/150‑1 (MQ=255) gaaCGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCGGCGTTATTGGCCGGTCGCTTGCGTCAAGGTGGACCTTGAAGCATTGCGTCGCGCTCGTGACCAGCtgt > 2:941437/1‑150 (MQ=255) actGACGTGGCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTaa < 2:76345/57‑1 (MQ=255) actGACGTGGCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTaa > 1:76345/3‑59 (MQ=255) actGACGTGGCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCGGCGTTATTGGCCGGTCGCTTGCGTCAAGGTGGACCTTGAAGCATTGCGTCGCGCTCGTGACCAGCTGTgg > 2:1354660/3‑147 (MQ=255) actGACGTGGCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCGGCGTTATTGGCCGGTCGCTTGCGTCAAGGTGGACCTTGAAGCATTGCGTCGCGCTCGTGACCAGCTGTgg < 1:1354660/145‑1 (MQ=255) gACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCGGCGTTATTGGCCGGTCGCTTGCGTCAAGGTGGACCTTGAAGCATTGCGTCGCGCTCGTGACCAGCTGTGGGCTGAg < 1:601039/150‑1 (MQ=255) | GCGTTCCAAGCCATCCGAGGTAAGTGGATCGTCGAGCTGGGCGAATTGGACAGCTTCAACAAGGCAGAGAGCACCAAGGCCAAGCAATTCTTCTCGGCA‑TCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCGGCGTTATTGGCCGGTCGCTTGCGTCAAGGTGGACCTTGAAGCATTGCGTCGCGCTCGTGACCAGCTGTGGGCTGAG > NC_002947/3423428‑3423718 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |