| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 321,743 | G→A | 13.6% | intergenic (+28/+430) | aguA → / ← PP_0267 | agmatine deiminase/ferric siderophore receptor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 321,743 | 0 | G | A | 13.6% | 100.3 / 7.2 | 44 | intergenic (+28/+430) | aguA/PP_0267 | agmatine deiminase/ferric siderophore receptor |
| Reads supporting (aligned to +/- strand): ref base G (24/14); new base A (2/4); total (26/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01 | |||||||||||
TCCTGGCAAGGATCTTCCCCGACCATGAGGTGGTGATGATCCCGGGCCGCGAGCTGCTCTTGGGTGGCGGCAACATTCACTGCCTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGATGGCACGGTCAGGCCGCGCCTCTGATCTGTAACAATACCTACGTAAATTAGCCGCTCACGGGCCAG > NC_002947/321597‑321892 | tCCTGGCAAGGATCTTCCCCGACCATGAGGTGGTGATGATCCCGGGCCGCGAGCTGCTCTTGGGTGGCGGCAACATTCACTGCCTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGa > 1:949269/1‑150 (MQ=255) aTCTTCCCCGACCATGAGGTGGTGATGATCCCGGGCCGCGAGCTGCTCTTGGGTGGCGGCAACATTCACTGCCTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCACATGACGGGCctctca < 1:143218/150‑3 (MQ=255) ccGACCATGAGGTGGTGATGATCCCGGGCCGCGAGCTGCTCTTGGGTGGCGGCAACATTCACTGCCTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTgcgc > 1:834356/1‑150 (MQ=255) ggtggtGATGATCCCGGGCCGCGAGCTGCTCTTGGGTGGCGGCAACATTCACTGCCTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGcc > 2:673679/1‑150 (MQ=255) tgatgaTCCCGGGCCGCGAGCTGCTCTTGGGTGGCGGCAACATTCACTGCCTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGCCGGTCTTTTTTGCGCGGGCGACGCCCGGaa > 1:1070857/1‑150 (MQ=255) tCCCGGGCCGCGAGCTGCTCTTGGGTGGCGGCAACATTCACTGCCTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAg > 1:1166925/1‑150 (MQ=255) cccGGGCCGCGAGCTGCTCTTGGGTGGCGGCAACATTCACTGACTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGc > 1:1316803/1‑150 (MQ=255) gctgctCTTGGGTGACGGCAACATTCACTGCCTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGCGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCGTACGatt > 2:1807/1‑150 (MQ=255) ctgctCTTGGGTGGCGGCAACATTCACTGCCTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGtttt < 2:1094194/150‑1 (MQ=255) tctTGGGTGGCGGCAACATTCACTGCCTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTAt > 1:371081/1‑150 (MQ=255) tctTGGGTGGCGGCAACATTCACTGCCTGACACAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTAt > 2:128452/1‑150 (MQ=255) ctTGGGTGGCGGCAACATTCACTGCCTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTAtt > 1:971709/1‑150 (MQ=255) gggTGGCGGCAACATTCACTGCCTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGtt > 2:1017626/1‑150 (MQ=255) ggTGGCGGCAACATTCACTGCCTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGttt > 1:381368/1‑150 (MQ=255) ggTGGCGGCAACATTCACTGCCTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGttt < 2:1268858/150‑1 (MQ=255) aaCATTCACTGCCTGACGCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTC‑TTTTGGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGa < 1:1055935/150‑1 (MQ=255) cATTCACTGCCTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGCCGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGAt < 1:460155/150‑1 (MQ=255) ccTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGATg < 2:381368/150‑1 (MQ=255) tGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGGGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGATGGc < 2:157749/150‑1 (MQ=255) gACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCg > 2:506296/1‑102 (MQ=255) gACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCg < 1:506296/102‑1 (MQ=255) ccAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGTCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGATGGCACgg > 2:828754/1‑150 (MQ=255) ccAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTg < 1:335191/116‑1 (MQ=255) ccAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTg > 2:335191/1‑116 (MQ=255) aaCAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGATGGCACGGTc > 1:1249835/1‑150 (MQ=255) aCAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGACGGCACGGTCa < 2:246912/150‑1 (MQ=255) ccgatctgTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGATGGCACGGTCAGGCCGCGcc > 2:351035/8‑150 (MQ=255) gcCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGATGGCACGGTCAGGCCGCGCctct > 2:263956/1‑150 (MQ=255) gTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGATGGCACGGTCAGGCCGCGCCTCTGATc < 2:834356/150‑1 (MQ=255) aGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGATGGCACGGTCAGGCCGCGCCTCTGATCTGTa > 1:97453/1‑150 (MQ=255) cccGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGATGGCACGGTCAGGCCGCGCCTCTGATCTGTAACAATACCt > 2:411701/1‑150 (MQ=255) cGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGATGGCACGGTCAGGCCGCGCCTCTGATCTGTAACAATACCTAc > 2:137140/1‑150 (MQ=255) gTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGATGGCACGGTCAGGCCGCGCCTCTGATCTGTAACAATACCTACg > 2:1287555/1‑150 (MQ=255) gaatgaGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGATGGCACGGTCAGGCCGCGCCTCTGATCTGTAACAATACCTACGTAAAt < 2:1316803/150‑1 (MQ=255) agagGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGATGGCACGGTCAGGCCGCGCCTCTGATCTGTAACAATACCTACGTAAATTAGcc < 1:1017626/150‑1 (MQ=255) agGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGATGGCACGGTCAGGCCGCGCCTCTGATCTGTAACAATACCTACGTAAATTAGCCGc > 1:663751/1‑150 (MQ=255) agGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGATGGCACGGTCAGGCCGCGCCTCTGATCTGTAACAATACCTACGTAAATTAGCCGc < 1:1287555/150‑1 (MQ=255) agGCCCGTCACATGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGa > 2:989946/1‑87 (MQ=255) agGCCCGTCACATGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGa < 1:989946/87‑1 (MQ=255) gCCCGTCACATGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGATGGCACGGTCAGGCCgcgc > 2:1043834/1‑112 (MQ=255) gCCCGTCACATGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGATGGCACGGTCAGGCCgcgc < 1:1043834/112‑1 (MQ=255) ccGTCACATGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGATGGCACGGTCAGGCCGCGCCTCTGATCTGTaa < 2:1014340/123‑1 (MQ=255) gTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGATGGCACGGTCAGGCCGCGCCTCTGATCTGTAACAATACCTACGTAAATTAGCCGCTCACgg > 2:1218357/1‑150 (MQ=255) cATGTGACGGGTCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGATGGCACGGTCAGGCCGCGCCTCTGATCTGTAACAATACCTACGTAAATTAGCCGCTCACGGGc < 2:1070857/150‑1 (MQ=255) gtgACGGGCCTCTTTTTTGCGCGGGCGATGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGATGGCACGGTCAGGCCGCGCCTCTGATCTGTAACAATACCTACGTAAATTAGCCGCTCACGGGCCAg > 1:971680/1‑150 (MQ=255) | TCCTGGCAAGGATCTTCCCCGACCATGAGGTGGTGATGATCCCGGGCCGCGAGCTGCTCTTGGGTGGCGGCAACATTCACTGCCTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGATGGCACGGTCAGGCCGCGCCTCTGATCTGTAACAATACCTACGTAAATTAGCCGCTCACGGGCCAG > NC_002947/321597‑321892 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |