Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 322,142 T→C 22.0% intergenic (+427/+31) aguA → / ← PP_0267 agmatine deiminase/ferric siderophore receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947322,1420TC22.0% 82.6 / 20.4 50intergenic (+427/+31)aguA/PP_0267agmatine deiminase/ferric siderophore receptor
Reads supporting (aligned to +/- strand):  ref base T (23/16);  new base C (7/4);  total (30/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.72e-01

GAAGCATCAGGGTCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAATGTTGCTCAATGCCGCCCGCA  >  NC_002947/321996‑322291
                                                                                                                                                  |                                                                                                                                                     
gAAGCATCAGGGTCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTcgc                                                                                                                                                    <  2:1219833/150‑1 (MQ=255)
gAAGCATCAGGGTCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTcgc                                                                                                                                                    >  1:514717/1‑150 (MQ=255)
     aTCAGGGTCAGGACGACCGACCCACGACGACTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAaga                                                                                                                                               <  2:514717/150‑1 (MQ=255)
       cAGGGTCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAgg                                                                                                                                             <  2:985828/150‑1 (MQ=255)
           gTCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAgg                                                                                                                                         >  1:534048/1‑150 (MQ=255)
                                            gggCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTcgc                                                                                                                                                    <  1:890072/106‑1 (MQ=255)
                                            gggCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTcgc                                                                                                                                                    >  2:890072/1‑106 (MQ=255)
                                                  tACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATTAGAAACTGTAGCTACCCGTca                                                                                                  >  2:895947/1‑150 (MQ=255)
                                                         aaGGGGTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGAGGGGGGGGGGGCCTGCCTCTTCGCGGGCATGCTCGCGAAGTGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAg                                                                                           <  2:674640/150‑1 (MQ=255)
                                                                gATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCATCTTCCCTGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTAcgc                                                                                    <  2:115380/150‑1 (MQ=255)
                                                                 aTCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTAcgcg                                                                                   >  1:818766/1‑150 (MQ=255)
                                                                  tCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGAGCATGCCCGCGAaga                                                                                                                                               >  2:457901/1‑89 (MQ=255)
                                                                  tCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGAGCATGCCCGCGAaga                                                                                                                                               <  1:457901/89‑1 (MQ=255)
                                                                  tCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTAACTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCCAACGGCTAcgccg                                                                                  >  2:813359/1‑148 (MQ=255)
                                                                    aGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGAt                                                                                >  2:932875/1‑150 (MQ=255)
                                                                      cGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACACAGCTACGCGGATCg                                                                              >  2:758558/1‑150 (MQ=255)
                                                                          gCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCa                                                                          >  2:986941/1‑150 (MQ=255)
                                                                           cAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACt                                                                                                                <  1:1199215/111‑1 (MQ=255)
                                                                           cAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACt                                                                                                                >  2:1199215/1‑111 (MQ=255)
                                                                           cAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATc                                                                               <  2:1038257/144‑1 (MQ=255)
                                                                           cAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATc                                                                               >  1:1038257/1‑144 (MQ=255)
                                                                                        gcTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGcgcc                                                            >  1:517207/1‑150 (MQ=255)
                                                                                              ggCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTg                                                      <  2:818766/150‑1 (MQ=255)
                                                                                                       gCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAg                                             >  2:880207/1‑150 (MQ=255)
                                                                                                         aCTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCa                                                                                                                                           >  2:930003/1‑54 (MQ=255)
                                                                                                         aCTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCa                                                                                                                                           <  1:930003/54‑1 (MQ=255)
                                                                                                         aCTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCt                                           <  1:932875/150‑1 (MQ=255)
                                                                                                           tGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACCGGCCAATTACTATCAACAATTGTAGCTGCTTGTTACCTCTATGCTATGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTgt                                         >  2:871691/1‑150 (MQ=25)
                                                                                                                gATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGtagtag                                    <  1:880207/150‑1 (MQ=255)
                                                                                                                 aTTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAgg                                   >  1:824758/1‑150 (MQ=255)
                                                                                                                          ttACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAg                          <  2:175335/149‑1 (MQ=255)
                                                                                                                              cTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAAt                      >  1:655719/1‑150 (MQ=255)
                                                                                                                                gCCTCTTCGCGAGCATGCCCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAATGt                    >  1:1071826/1‑150 (MQ=255)
                                                                                                                                  ctctTCGCGAGCATGCCCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGt                                                    >  2:990403/1‑116 (MQ=255)
                                                                                                                                  ctctTCGCGAGCATGCCCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGt                                                    <  1:990403/116‑1 (MQ=255)
                                                                                                                                   tctTCGCGAGCATGCCCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTAcgcg                                                                                   <  2:497798/84‑1 (MQ=255)
                                                                                                                                   tctTCGCGAGCATGCCCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTAcgcg                                                                                   >  1:497798/1‑84 (MQ=255)
                                                                                                                                   tctTCGCGAGCATGCCAGCGCAGATGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAATGTTGc                 >  2:486875/1‑150 (MQ=255)
                                                                                                                                       cgcgAGCATGCCCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGa                                                                                                                    <  1:1124610/47‑1 (MQ=17)
                                                                                                                                       cgcgAGCATGCCCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGa                                                                                                                    >  2:1124610/1‑47 (MQ=17)
                                                                                                                                          gggCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACaa                                                                                            >  2:1336928/1‑68 (MQ=255)
                                                                                                                                          gggCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACaa                                                                                            <  1:1336928/68‑1 (MQ=255)
                                                                                                                                          gggCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAATGTTGCTCAATgc          >  1:965054/1‑150 (MQ=255)
                                                                                                                                           ggCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAATGTTGCTCAATgcc         >  1:276462/1‑150 (MQ=255)
                                                                                                                                            gCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTAccc                                                                                                      <  1:437048/56‑1 (MQ=255)
                                                                                                                                            gCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTAccc                                                                                                      >  2:437048/1‑56 (MQ=255)
                                                                                                                                              aTGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTgt                                         >  2:274000/1‑115 (MQ=255)
                                                                                                                                              aTGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTgt                                         <  1:274000/115‑1 (MQ=255)
                                                                                                                                              aTGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAATGTTGCTCAATgccgcc      >  1:351801/1‑150 (MQ=255)
                                                                                                                                              aTGCCCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAATGTTGCTCAATgccgcc      >  1:561274/1‑150 (MQ=255)
                                                                                                                                                  tCGCGAAGAGGCAGGTACAGGCAACTTAAACCCACAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAATGTTGCTCAATGCCGCCCGca  <  2:824758/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GAAGCATCAGGGTCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAATGTTGCTCAATGCCGCCCGCA  >  NC_002947/321996‑322291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑