| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,533,413 | A→G | 6.8% | E16E (GAA→GAG) | atoA → | 3‑oxoacid CoA‑transferase subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,533,413 | 0 | A | G | 6.8% | 174.7 / 2.4 | 59 | E16E (GAA→GAG) | atoA | 3‑oxoacid CoA‑transferase subunit A |
| Reads supporting (aligned to +/- strand): ref base A (30/25); new base G (2/2); total (32/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01 | |||||||||||
TGACGAAAACAAAATTAACAAATCAGTCGCTTGCGGTGAAGATGTCTGCACACGCTCACCGACAGCCTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAGCGGCGCGGCGTCAAGGGCCTGACCGTGGTTTCCAACAACTGCGGCGTGGACGGTTTTGGTCT > NC_002947/3533265‑3533556 | tGACGAAAACAAAATTAACAAATCAGTCGCTTGCGGTGAAGATGTCTGCACACGCTCACCGACAGCCTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAg < 2:403473/150‑1 (MQ=255) gAAAACAAAATTAACAAATCAGTCGCTTGCGGTGAAGATGTCTGCACACGCTCACCGACAGCCTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCt < 1:652117/150‑1 (MQ=255) aaTTAACAAATCAGTCGCTTGCGGTGAAGATGTCTGCACACGCTCACCGACAGCCTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGANAAGNGNGATTCAACCGATGAGCAGGCCCTCGAAGGCCTGACCGaca > 2:337795/1‑150 (MQ=255) aTTAACAAATCAGTCGCTTGCGGTGAAGATGTCTGAAAACGCTAATCCTCAGCCCCGTCGGGTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGacaa < 1:1386422/150‑1 (MQ=255) aCAAATCAGTCGCTTGCGGTGAAGATGTCTGCACACGCTCACCGACAGCCTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATg > 2:1322858/1‑150 (MQ=255) aaaTCAGTCGCTTGCGGTGAAGATGTCTGCACACGCTCACCGACAGCCTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCTTCGAGGGCCTgctcat > 1:1192905/1‑136 (MQ=255) aaaTCAGTCGCTTGCGGTGAAGATGTCTGCACACGCTCACCGACAGCCTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCTTCGAGGGCCTgctcat < 2:1192905/141‑6 (MQ=255) aaaTCAGTCGCTTGCGGTGAAGATGTCTGCACACGCTCACCGACAGCCTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGAc > 2:640412/1‑150 (MQ=255) aaaTCAGTCGCTTGCGGTGAAGATGTCTGCACACGCTCACCGACAGCCTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGAc > 2:638198/1‑150 (MQ=255) aaTCAGTCGCTTGCGGTGAAGATGTCTGCACACGCTCACCGACAGCCTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACg < 1:1322858/150‑1 (MQ=255) aaTCAGTCGCTTGCGGTGAAGATGTCTGCACACGCTCACCGACAGCCTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACg < 1:227975/150‑1 (MQ=255) aTCAGTCGCTTGCGGTGAAGATGTCTGCACACGCTCACCGACAGCCTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACgg > 2:1323716/1‑150 (MQ=255) aTCAGTCGCTTGCGGTGAAGATGTCTGCACACGCTCACCGACAGCCTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACgg > 1:186382/1‑150 (MQ=255) gTCGCTTGCGGTGAAGATGTCTGCACACGCTCACCGACAGCCTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTANt < 1:337795/150‑1 (MQ=255) cGGTGAAGATGTCTGCACACGCTCACCGACAGCCTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCggtg < 2:484794/150‑1 (MQ=255) aGATGTCTGCACACGCTCACCGACAGCCTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCg < 1:1323716/150‑1 (MQ=255) aTGTCTGCACACGCTCACCGACAGCCTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGc < 2:861351/150‑1 (MQ=255) aGCCTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCgg > 1:34372/1‑127 (MQ=255) aGCCTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCgg < 2:34372/127‑1 (MQ=255) aGCCTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAAcc < 1:175677/150‑1 (MQ=255) ccTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCtt > 2:853789/1‑150 (MQ=255) ggTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCg > 1:540887/1‑150 (MQ=255) acaacTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTc < 2:202733/110‑1 (MQ=255) acaacTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTc > 1:202733/1‑110 (MQ=255) acaacTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAg > 2:1048904/1‑142 (MQ=255) acaacTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAg < 1:1048904/142‑1 (MQ=255) acaacTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCa > 2:780496/1‑150 (MQ=255) acaacTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCa > 2:1347361/1‑150 (MQ=255) acaacTACAAAAGGCGGATCAGCATGGCAATTCTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCa > 1:398029/1‑150 (MQ=255) aacTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAg < 1:780496/150‑1 (MQ=255) aacTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAg > 1:818036/1‑150 (MQ=255) acTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAgc < 1:638198/150‑1 (MQ=255) aCAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAgcggc < 2:818036/150‑1 (MQ=255) aaaaGTCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATc > 2:951610/1‑117 (MQ=255) aaaaGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATc < 1:951610/117‑1 (MQ=255) aGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAgcggcgcggc < 1:640412/150‑1 (MQ=255) tcatcaTGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCa < 1:714287/123‑1 (MQ=255) tCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCa > 2:714287/1‑123 (MQ=255) aGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAGCGGCGCGGCGTCAAGGGc < 1:853789/150‑1 (MQ=255) cATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAGCGGCGCGGCGTCCAGGGCCt > 2:494952/1‑150 (MQ=255) aTGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAGCGGCGCGGCGTCAAGGGCCTg > 1:944392/1‑150 (MQ=255) cGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAGCGGCGCGGCGTCAAGGGCCTGACCGt > 1:210278/1‑150 (MQ=255) gACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCtgt < 2:264886/82‑1 (MQ=255) gACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCtgt > 1:264886/1‑82 (MQ=255) aaGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCTGCATCCCGGAAAACCTTATCAGCGAAATCAAGCGGCGCGGCGTCAAGGGCCTGACCGTGGTTTCcaac > 1:236432/1‑150 (MQ=255) aaGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAGCGGCGCGGCGTCAAGGGCCTGACCGTGGTTTCcaac > 2:87020/1‑150 (MQ=255) gcgcGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAAcc < 1:1377138/95‑1 (MQ=255) gcgcGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAAcc > 2:1377138/1‑95 (MQ=255) ttGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAGCGGCGCGGCGTCAAGGGCCTGACCGTGGTTTCCAACAACTgcg > 1:646882/1‑150 (MQ=255) aaCCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAGCGGCGCGGCGTCAAGGGCCTGACCGTGGTTTCCAACAACTGCGGCGt > 2:527164/1‑150 (MQ=255) aaCCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAGCGGCGCGGCGTCAAGGGCCTGACCGTGGTTTCCAACAACTGCGGCGt > 2:386420/1‑150 (MQ=255) aCCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAGCGGCGCGGCGTCAAGGGCCTGACCGTGGTTTCCAACAACTGCGGCGTg < 2:944392/150‑1 (MQ=255) gAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGCAAACCTTATCAGCGAGATCAAGCGGCGCGGCGTCAAGGGCCTGACCGTGGTTTTCAACAACTGCAGCGCGGACGGt > 2:614997/1‑150 (MQ=255) aGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAGCGGCGCGGCGTCAAGGGCCTGACCGTGGTTTCCAACAACTGCGGCGTGGACGGtt < 2:1293093/150‑1 (MQ=255) tcAGGCCTTCGAGGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAGCGGCGCGGCGTCAAGGGCCTGACCGTGGTTTCcaa > 1:417558/2‑129 (MQ=255) tcAGGCCTTCGAGGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAGCGGCGCGGCGTCAAGGGCCTGACCGTGGTTTCcaa < 2:417558/128‑1 (MQ=255) gCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAgcggcgcgg > 1:1157788/1‑98 (MQ=255) gCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAgcggcgcgg < 2:1157788/98‑1 (MQ=255) ccTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAGCGGCGCGGCGTCAAGGGCCTGACCGTGGTTTCCAACAACTGCGGCGTGGACGGTTTTGGTCt > 2:1069246/1‑150 (MQ=255) | TGACGAAAACAAAATTAACAAATCAGTCGCTTGCGGTGAAGATGTCTGCACACGCTCACCGACAGCCTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAGCGGCGCGGCGTCAAGGGCCTGACCGTGGTTTCCAACAACTGCGGCGTGGACGGTTTTGGTCT > NC_002947/3533265‑3533556 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |