Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,542,364 C→T 9.0% P134P (CCG→CCA)  galE ← UDP‑glucose 4‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,542,3640CT 9.0% 217.1 / 10.1 78P134P (CCG→CCA) galEUDP‑glucose 4‑epimerase
Reads supporting (aligned to +/- strand):  ref base C (40/31);  new base T (4/3);  total (44/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01

AGATGGGTTTCCGGCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGCGACGTTGTTCTG  >  NC_002947/3542215‑3542513
                                                                                                                                                     |                                                                                                                                                     
aGATGGGTTTCCGGCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATAc                                                                                                                                                       >  2:44891/1‑150 (MQ=255)
    gggTTTCCGGCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCGTATGGGt                                                                                                                                                   <  2:880845/150‑1 (MQ=255)
           cGGCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCgg                                                                                                                                            >  1:1209535/1‑150 (MQ=255)
                                    ccTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCatcgatc                                                                                                                   <  2:1209535/150‑1 (MQ=255)
                                     ctaccGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCatcgatcg                                                                                                                  >  1:1420777/4‑150 (MQ=255)
                                      ttCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGgtgtgt                                                                                                                               <  2:632321/136‑1 (MQ=255)
                                      ttCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGgtgtgt                                                                                                                               >  1:632321/1‑136 (MQ=255)
                                           ggTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTAAAACAATAGCAATAAACGAAGTCCTCAAGAAGCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGt                                                                                                            >  1:1243745/1‑150 (MQ=255)
                                             tCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTCCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTAc                                                                                                          >  2:1248791/1‑150 (MQ=255)
                                                     cGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGtt                                                                                                  <  2:1420777/150‑1 (MQ=255)
                                                              gAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGcc                                                                                               <  2:814492/144‑1 (MQ=255)
                                                              gAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGcc                                                                                               >  1:814492/1‑144 (MQ=255)
                                                               ataTAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTAAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATAcg                                                                                        >  2:356265/3‑150 (MQ=255)
                                                               aaaTAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATAcg                                                                                        >  2:1295560/1‑150 (MQ=255)
                                                               aaaTAGCGCAGGCTTACCGACTTCAAACCATAGGCACGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGATCAACCCGTATGGGt                                                                                                                                                   <  1:458996/91‑1 (MQ=255)
                                                               aaaTAGCGCAGGCTTACCGACTTCAAACCATAGGCACGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGATCAACCCGTATGGGt                                                                                                                                                   >  2:458996/1‑91 (MQ=255)
                                                                 aTAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATAcggc                                                                                      <  2:1216092/150‑1 (MQ=255)
                                                                      gcAGGCTTACCGCCTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGc                                                                                                       <  2:233125/128‑1 (MQ=255)
                                                                      gcAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGc                                                                                                       >  1:233125/1‑128 (MQ=255)
                                                                             tACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCgaaga                                                                          >  2:566848/1‑150 (MQ=255)
                                                                                cGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTGATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAaaaaaa                                                                       >  2:715178/1‑150 (MQ=255)
                                                                                 gACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCgaa                                                                            >  1:520394/1‑144 (MQ=255)
                                                                                 gACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGATCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCgaa                                                                            <  2:520394/144‑1 (MQ=255)
                                                                                  aCTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAaca                                                                     >  1:555163/1‑150 (MQ=255)
                                                                                   cTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCgaag                                                                           >  1:636018/1‑143 (MQ=255)
                                                                                   cTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCgaag                                                                           <  2:636018/143‑1 (MQ=255)
                                                                                    ttCAGACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACAcc                                                                   >  2:595294/1‑150 (MQ=255)
                                                                                    ttCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTgg                                                                                                                                    >  1:118191/1‑85 (MQ=255)
                                                                                    ttCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTgg                                                                                                                                    <  2:118191/85‑1 (MQ=255)
                                                                                    atCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACAcc                                                                   >  2:979528/2‑150 (MQ=255)
                                                                                     tCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCa                                                                  <  2:555163/150‑1 (MQ=255)
                                                                                      cAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAg                                                                 <  2:1243745/150‑1 (MQ=255)
                                                                                          ccATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGc                                                             >  1:779941/1‑150 (MQ=255)
                                                                                           cATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGcc                                                            >  2:947485/1‑150 (MQ=255)
                                                                                            aTAGGCCCGGTCTAAGTCCTCAAGAATCTGCTAGACCATACATTTGCTCAACCCATACGGGTTGATCGGCCCCTCAGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCt                                                           <  1:1386487/150‑1 (MQ=255)
                                                                                             tAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTg                                                          >  2:1250436/1‑150 (MQ=255)
                                                                                             tAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTg                                                          >  1:965770/1‑150 (MQ=255)
                                                                                               ggCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGAt                                                        <  1:809172/150‑1 (MQ=255)
                                                                                               ggCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGAt                                                        <  1:44891/150‑1 (MQ=255)
                                                                                                 cccGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACATCAGGTGCCTGATGc                                                      >  2:1333348/1‑150 (MQ=255)
                                                                                                   cGGTCGAAGTCCTCAAGAATCTGCTCGAGCTTCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGACGTATACGGCGGCACTCGAAGCAAACACCAGGTGCCTGATGCCg                                                    <  1:715178/150‑1 (MQ=255)
                                                                                                      tCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCa                                                 >  1:1094355/1‑150 (MQ=255)
                                                                                                         aaGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTg                                              >  2:914523/1‑150 (MQ=255)
                                                                                                            tCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCAtt                                               <  1:950882/146‑1 (MQ=255)
                                                                                                            tCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCAtt                                               >  2:950882/1‑146 (MQ=255)
                                                                                                               tCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATg                                        <  1:979528/150‑1 (MQ=255)
                                                                                                                 aaGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGc                                      >  1:1329850/1‑150 (MQ=255)
                                                                                                                   gAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCt                                    <  1:753727/150‑1 (MQ=255)
                                                                                                                          cTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGcagcag                             >  2:1008269/1‑150 (MQ=255)
                                                                                                                           tCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAgg                            <  1:1008269/150‑1 (MQ=255)
                                                                                                                           tCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAgg                            <  1:566848/150‑1 (MQ=255)
                                                                                                                             gACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGa                          >  2:601121/1‑150 (MQ=255)
                                                                                                                                 atccatTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAgg                      >  2:616124/1‑150 (MQ=255)
                                                                                                                                  tccatTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCAc                                                                                 >  2:313797/1‑90 (MQ=255)
                                                                                                                                  tccatTTGCGGAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCAc                                                                                 <  1:313797/90‑1 (MQ=255)
                                                                                                                                   ccatTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGt                    <  1:356265/150‑1 (MQ=255)
                                                                                                                                    catTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTg                   <  1:1250436/150‑1 (MQ=255)
                                                                                                                                     atTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTgg                  >  2:924822/1‑150 (MQ=255)
                                                                                                                                     atTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTgg                  >  2:924809/1‑150 (MQ=255)
                                                                                                                                     atTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTg                   >  2:1395119/1‑149 (MQ=255)
                                                                                                                                     atTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTg                   <  1:1395119/149‑1 (MQ=255)
                                                                                                                                      gccgatCAACCCGTATGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGc                 >  1:899520/6‑150 (MQ=255)
                                                                                                                                         gatCAACCCGTATGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCt                <  2:898499/146‑1 (MQ=255)
                                                                                                                                         gatCAACCCGTATGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCt                >  1:898499/3‑148 (MQ=255)
                                                                                                                                         gatCAACCCGTATGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGc              >  2:773302/3‑150 (MQ=255)
                                                                                                                                         gCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGAACATGGCCTGCAGCAGGGAAAGGGTGGCTGc              <  1:616124/150‑1 (MQ=255)
                                                                                                                                           tCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGCGa            >  1:1298410/1‑150 (MQ=255)
                                                                                                                                             aaCCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATAcggcg                                                                                     >  1:1336950/1‑75 (MQ=255)
                                                                                                                                             aaCCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATAcggcg                                                                                     <  2:1336950/75‑1 (MQ=255)
                                                                                                                                             aaCCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGCGACg          >  2:1324441/1‑150 (MQ=255)
                                                                                                                                             aaCCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGCGACg          <  1:947485/150‑1 (MQ=255)
                                                                                                                                             aaCCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGCGACg          >  1:50768/1‑150 (MQ=255)
                                                                                                                                              aCCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAgg                                                                <  1:1265907/95‑1 (MQ=255)
                                                                                                                                              aCCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAgg                                                                >  2:1265907/1‑95 (MQ=255)
                                                                                                                                              aCCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGCGACgt         >  2:601476/1‑150 (MQ=255)
                                                                                                                                                ccATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACATCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGCGACgttg       <  1:1333348/150‑1 (MQ=255)
                                                                                                                                                  aTACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGCGACgttgtt     >  2:1311686/1‑150 (MQ=255)
                                                                                                                                                   tACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACGAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGCGACGTTATTc    <  1:1248791/150‑1 (MQ=255)
                                                                                                                                                    aCGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGCGACGTTGTTCt   <  1:1295560/150‑1 (MQ=255)
                                                                                                                                                     cGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGCGACGTTGTTCTg  <  1:1311686/150‑1 (MQ=255)
                                                                                                                                                     cGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGCGACGTTGTTCTg  <  1:1324441/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGATGGGTTTCCGGCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGCGACGTTGTTCTG  >  NC_002947/3542215‑3542513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑