| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,584,942 | A→C | 10.0% | intergenic (+46/‑89) | benC → / → benD | benzoate 1,2‑dioxygenase electron transfer component/1,6‑dihydroxycyclohexa‑2,4‑diene‑1‑carboxylate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,584,942 | 0 | A | C | 10.0% | 126.8 / 6.9 | 50 | intergenic (+46/‑89) | benC/benD | benzoate 1,2‑dioxygenase electron transfer component/1,6‑dihydroxycyclohexa‑2,4‑diene‑1‑carboxylate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (22/23); new base C (3/2); total (25/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 | |||||||||||
GCGGCCCGCCACCCATGGTGGAAGCGGTCAGCCAGTACGTGCGTGAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGATTCCAAGGCAAGGTTGCCCTGGTCACCGGTGCCGCCCAAGG > NC_002947/3584796‑3585083 | gCGGCCCGCCACCCATGGTGGAAGCGGTCAGCCAGTACGTGCGTGAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGcc < 2:1064643/150‑1 (MQ=255) cGGCCCGCCACCCATGGTGGAAGCGGTCAGCCAGTACGTGCGTGAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGccc < 1:834047/150‑1 (MQ=255) cGCCACCCATGGTGGAAGCGGTCAGCCAGTACGTGCGTGAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCTCGCCCGCTcc < 2:1364310/150‑1 (MQ=255) gCCACCCATGGTGGAAGTGGTCAGCCAGTACGTGCGTGAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGTGa < 1:1289574/140‑1 (MQ=255) gCCACCCATGGTGGAAGTGGTCAGCCAGTACGTGCGTGAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGa > 2:1289574/1‑140 (MQ=255) aCCCATGGTGGAAGCGGTCAGCCAGTACGTGCGTGAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCcaca > 2:165812/1‑150 (MQ=255) aCCCATGGTGGAAGCGGTCAGCCAGTACGTGCGTGAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCcaca > 1:618724/1‑150 (MQ=255) ccATGGTGGAAGCGGTCAGCCAGTACGTGCGTGAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGt > 1:588358/1‑150 (MQ=255) cATGGTGGAAGCGGTCAGCCAGTACGTGCGTGAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTg > 1:178674/1‑150 (MQ=255) aTGGTGGAAGCGGTCAGCCAGTACGTGCGTGAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGa > 2:906418/1‑150 (MQ=255) tggAAGCGGTCAGCCAGTACGTGCGTGAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATcgc < 1:1131688/150‑1 (MQ=255) cgtgAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCaa < 1:349122/150‑1 (MQ=255) gtgAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAag > 1:1012840/1‑150 (MQ=255) gCATTACCCCGGCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTtgct > 1:520953/1‑150 (MQ=255) ccGGCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGGTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAgg < 1:165812/150‑1 (MQ=255) ccGGCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCGTTGAGGAAGAGAGTTGCTGCCACAgg > 1:205503/1‑150 (MQ=255) ggCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGt < 2:1012840/150‑1 (MQ=255) ggCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGt > 1:402151/1‑150 (MQ=255) ttCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTcgc < 2:520953/150‑1 (MQ=255) ttCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTcgc < 1:906418/150‑1 (MQ=255) ctacGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAg < 2:252336/135‑1 (MQ=255) ctacGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAg > 1:252336/1‑135 (MQ=255) gagaAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGgag > 1:624753/1‑150 (MQ=255) gTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTa > 2:18924/1‑150 (MQ=255) tttGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAagaga < 1:1288868/112‑1 (MQ=255) tttGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAagaga > 2:1288868/1‑112 (MQ=255) ttGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACa < 2:402151/150‑1 (MQ=255) ggctgcggcCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATg < 2:197581/150‑1 (MQ=255) cggcCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAg < 1:408086/87‑1 (MQ=255) cggcCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAg > 2:408086/1‑87 (MQ=255) gcCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAacaaca > 1:230966/1‑150 (MQ=255) gcCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGCCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAacaaca < 2:953950/150‑1 (MQ=255) gCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGATTCca > 2:645363/1‑150 (MQ=255) ccTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAagagag < 2:810086/90‑1 (MQ=255) ccTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAagagag > 1:810086/1‑90 (MQ=255) ccTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTtgc < 1:355247/94‑1 (MQ=255) ccTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTtgc > 2:355247/1‑94 (MQ=255) ccTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGATTCcaag > 1:375607/1‑148 (MQ=255) ccTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGATTCcaag < 2:375607/148‑1 (MQ=255) gATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGATTCcaaggcaag < 2:624753/150‑1 (MQ=255) aTTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGATTCcaaggcaagg < 1:18924/150‑1 (MQ=255) tCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTg > 1:137106/1‑112 (MQ=255) tCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTg < 2:137106/112‑1 (MQ=255) aaaGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGATTCCAAGGCAAGGTTGc > 1:1235115/1‑150 (MQ=255) aTACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGATTCCAAGGCAAGGTTGCCCTGGt < 2:618724/150‑1 (MQ=255) acTGTGGGAGCGGGCTCGCCCGCTCCCGCAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGATTCCAAGGCAAGGTTGCCCTGGTCACCGGTgcc > 2:1133792/2‑150 (MQ=255) tgGGAGCGGGCTCGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTgg > 2:1229668/1‑91 (MQ=255) tgGGAGCGGGCTCGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTgg < 1:1229668/91‑1 (MQ=255) gggAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGATTCCAAGGCAAGGTTGcc < 2:328635/130‑1 (MQ=255) gggAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGATTCCAAGGCAAGGTTGcc > 1:328635/1‑130 (MQ=255) gAGCGGGCTCGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTACGGAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGATTCCAAGGCAAGGTTGCCCTGGTCACCGGTGCCGCCCAAg > 1:615378/1‑150 (MQ=255) aGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGATTCCAAGGCAAGGTTGCCCTGGTCACCGGTGCCGCCCAAgg < 2:230966/150‑1 (MQ=255) | GCGGCCCGCCACCCATGGTGGAAGCGGTCAGCCAGTACGTGCGTGAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGATTCCAAGGCAAGGTTGCCCTGGTCACCGGTGCCGCCCAAGG > NC_002947/3584796‑3585083 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |