| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,590,228 | T→C | 11.1% | F236L (TTT→CTT) | nicP‑I → | porin‑like protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,590,228 | 0 | T | C | 11.1% | 93.3 / 3.5 | 36 | F236L (TTT→CTT) | nicP‑I | porin‑like protein |
| Reads supporting (aligned to +/- strand): ref base T (18/14); new base C (2/2); total (20/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.70e-01 | |||||||||||
CAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGTTCAATGCCAACCAAAGCAGCGTCAGCCTTTGGTACGGTCAGCTGGAGGACATCTACCAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGG > NC_002947/3590080‑3590376 | cAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGTTCAATGCCAACCAAAGCAGCGTCAGCCTTTGGTACGGTCAGCTGGAGGACATCTACCAACAAGGCtt > 2:1383985/1‑150 (MQ=255) aaGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGTTCAATGCCAACCAAAGCAGCGTCAGCCTTTGGTACGGTCAGCTGGAGGACATCTACCAACAAGGCttt < 2:1389020/150‑1 (MQ=255) aaCGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGTTCAATGCCAACCAAAGCAGCGTCAGCCTTTGGTACGGTCAGCTGGAGGACATCTACCAACAAGGCTTTGTTGGc < 2:1042844/150‑1 (MQ=255) gCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACACGTTCAACTATGTGGGTGGGGACTATGCGTTCAATGCCAACCAAAGCAGCGTCAGCCTTGGGTACGGTCAGCTGGAGGACATCTACCAACAAGGCTTTGTTGGCCTGAAGca > 1:1152569/1‑150 (MQ=255) cTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGTTCAATGCCAACCAAAGCAGCGTCAGCCTTTGGTACGGTCAGCTGGAGGACATCTACCAACAAGGCTTTGTTGGCCTGAAGcac > 1:582687/1‑150 (MQ=255) tCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGTTCAATGCCAACCAAAGCAGCGTCAGCCTTTGGTACGGTCAGCTGGAGGACATCTACCAACAAGGCTTTGTTGGCCTGAAGcaca < 2:90653/150‑1 (MQ=255) cGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGTTCAATGCCAACCAAAGCAGCGTCAGCCTTTGGTACGGTCAGCTGGAGGACATCTACCAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCg > 2:1107671/1‑150 (MQ=255) gACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTAGGCGTTCAATGCCAACCAAAGCAGCGTCAGCCGTTGGTACGGTCAGCTGGAGGACATCTACCAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACt < 1:1383985/150‑1 (MQ=255) aaGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGTTCAATGCCAACCAAAGCAGCGTCAGCCTTTGGTACGGTCAGCTGGAGGACATCTACCAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAg < 1:233020/150‑1 (MQ=255) ttCAACTATGTGGGTGGGGACTCTGCGCTCAATGCCAACCAAGGCATCTTCAGCGTTTGGGGCGGTCAGCTGGGGGACATCTACCAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTg > 2:1231864/1‑150 (MQ=255) tCAACTATGTGGGTGGGGACTATGCGTTCAATGCCAACCAAAGCAGCGTCAGCCTTTGGTACGGTCAGCTGGAGGACATCTACCAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGTCGACTGGGTACTCAGTGCCAACCTgg < 1:1107671/150‑1 (MQ=255) tgggtgggGACTATGCGTTCAATGCCAACCAAAGCAGCGTCAGCCTTTGGTACGGTCAGCTGGAGGACATCTACCAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCa > 2:525839/1‑150 (MQ=255) tATGCGTTCAATGCCAACCAAAGCAGCGTCAGCCTTTGGTACGGTCAGCTGGAGGACATCTACCAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCg > 2:82909/1‑103 (MQ=255) tATGCGTTCAATGCCAACCAAAGCAGCGTCAGCCTTTGGTACGGTCAGCTGGAGGACATCTACCAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCg < 1:82909/103‑1 (MQ=255) tGCCAACCAAAGCAGCGTCAGCCTTTGGTACGGTCAGCTGGAGGACATCTACCAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCAcc > 2:931462/1‑130 (MQ=255) tGCCAACCAAAGCAGCGTCAGCCTTTGGTACGGTCAGCTGGAGGACATCTACCAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCAcc < 1:931462/130‑1 (MQ=255) ccaaAGCAGCGTCAGCCTTTGGTACGGTCAGCTGGAGGACATCTACCAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCgctgct > 2:1113358/1‑150 (MQ=255) cTTTGGTACGGTCAGCTGGAGGACATCTACCAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAAc < 1:1113358/150‑1 (MQ=255) tttGGTACGGTCAGCTGGAGGACATCTACCAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAAcc > 2:1201278/1‑150 (MQ=255) tGGTACGGTCAGCTGGAGGACATCTACCAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAg < 1:525839/150‑1 (MQ=255) gTACGGTCAGCTGGAGGACATCTACCAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGc < 2:1386225/150‑1 (MQ=255) gTACGGTCAGCTGGAGGACATCTACCAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGAAAACATGGGTTCTTTAACGGCAAGAGCTCAAGGCTATGCGCGGCTTTGCAACATCGACAAGCAggg > 2:1085120/1‑149 (MQ=255) aCGGTCAGCTGGAGGACATCTACCAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGt < 2:430421/150‑1 (MQ=255) ggTCAGCTGAAGGACATCTACCAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGGTATTTCACCGCACGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGttc > 2:663797/1‑150 (MQ=255) tGGAGGACATCTACCAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGc < 2:1152569/150‑1 (MQ=255) tGGAGGACATCTACCAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGc > 2:466043/1‑150 (MQ=255) ggaggaCATCTACCAACAAGGCTTTGTTGGCCTGAAGCATTGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCt < 1:1085120/150‑1 (MQ=255) ggaCATCTACCAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGtt > 2:980488/1‑150 (MQ=255) ggaCATCTACCAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGtt > 1:717969/1‑150 (MQ=255) ttcaggccAACAAAGCCTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCa > 2:1180046/7‑149 (MQ=255) ttcaggccAACAAAGCCTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCa < 1:1180046/143‑1 (MQ=255) cATCTACCAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCAc > 1:350263/1‑150 (MQ=255) tACCAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGcc > 1:1194166/1‑150 (MQ=255) gccAACAAAGCCTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTgg > 1:645304/2‑108 (MQ=255) gccAACAAAGCCTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTgg < 2:645304/107‑1 (MQ=255) aCCAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGccc > 2:362509/1‑150 (MQ=255) acaaGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCa > 1:898092/1‑150 (MQ=255) cTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCgg < 2:582687/150‑1 (MQ=255) | CAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGTTCAATGCCAACCAAAGCAGCGTCAGCCTTTGGTACGGTCAGCTGGAGGACATCTACCAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGG > NC_002947/3590080‑3590376 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |