| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 325,161 | C→G | 7.8% | P149A (CCA→GCA) | oprQ → | outer‑membrane porin D |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 325,161 | 0 | C | G | 7.8% | 159.4 / 5.0 | 52 | P149A (CCA→GCA) | oprQ | outer‑membrane porin D |
| Reads supporting (aligned to +/- strand): ref base C (21/26); new base G (2/2); total (23/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01 | |||||||||||
GCGTCGACTTCTTCAAGCCGCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGTTCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATCCAGGGCCTGGAACTGAACGCCGGCCGCTTCACCCAGGAAGCGCGCAAGAGCGCCGAGCGCCGTGATGGTGGT > NC_002947/325018‑325307 | gcgTCGACTTCTTCAAGCCGCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGTTCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGtgc > 1:255065/1‑150 (MQ=255) gTCGACTTCTTCAAGCCGCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGTTCCGCATTTCAAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGtgctg > 1:95054/1‑150 (MQ=255) ttcttcAAGCCGCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGTTCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGc > 2:175795/1‑136 (MQ=255) ttcttcAAGCCGCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGTTCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGc < 1:175795/136‑1 (MQ=255) ttcttcAAGCCGCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGTTCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTac < 1:287879/150‑1 (MQ=255) ttcAAGCCGCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGTTCCGCATTTCCAACACCGTGCTGAAGTACGGTGGCCATATGCGAGCACTTCCAGTGCTGCAGTacgac > 2:74306/1‑150 (MQ=255) cGCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGTTCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCAc < 2:255065/150‑1 (MQ=255) aaaCCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGTTCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTctgc < 1:47998/150‑1 (MQ=255) caccaATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGTTCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGcc > 2:38532/1‑150 (MQ=255) aTGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGGGGTGGTGCTGCTGTGAAGTTCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCGGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGaaa > 1:1357945/1‑150 (MQ=255) tGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGTTCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAg < 1:767302/150‑1 (MQ=255) cGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGTTCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGcc > 2:770081/1‑141 (MQ=255) cGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGTTCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGcc < 1:770081/141‑1 (MQ=255) ggCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGTTCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCAcc < 2:960602/150‑1 (MQ=255) aaCGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGTTCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGc > 2:644213/1‑150 (MQ=255) ccAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGTTCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATg < 2:923272/117‑1 (MQ=255) ccAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGTTCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATg > 1:923272/1‑117 (MQ=255) aaCCTGGCGCGTGGTGGTGCTGCTGTGAAGTTCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGc > 1:1225319/1‑123 (MQ=255) aaCCTGGCGCGTGGTGGTGCTGCTGTGAAGTTCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGc < 2:1225319/123‑1 (MQ=255) aaCCTGGCGCGTGGTGGTGCTGCTGTGAAGTTCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCaaa < 1:660202/150‑1 (MQ=255) cTGGCGCGTGGTGGTGCTGCTGTGAAGTTCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAagag > 2:1189095/1‑150 (MQ=255) cTGGCGCGTGGTGGTGCTGCTGTGAAGTTCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAagag > 1:959718/1‑150 (MQ=255) tGGCGCGTGGTGGTGCTGCTGTGAAGTTCCGCATTTCCAACACCGTGCTTAAGTACGGTGACCAGATGCCAGAACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAgaga < 1:94530/150‑1 (MQ=255) tGGCGCGTGGTGGTGCTGCTGTGAAGTTCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCAc > 1:345193/1‑102 (MQ=255) tGGCGCGTGGTGGTGCTGCTGTGAAGTTCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCAc < 2:345193/102‑1 (MQ=255) tGGCGCGTGGTGGTGCTGCTGTGAAGTTCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAgaga > 1:1272060/1‑150 (MQ=255) tggtgCTGCTGTGAAGTTCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCa < 2:706537/103‑1 (MQ=255) tggtgCTGCTGTGAAGTTCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCa > 1:706537/1‑103 (MQ=255) gctgctGTGAAGTTCCGCATTTCCAACACCGTGATCCTGCACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATCCAGGGCCTGGaa < 1:74306/150‑1 (MQ=255) tgtgAAGTTCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATCCAGGGCCTGGAACTGaa > 1:659725/1‑150 (MQ=255) gtgAAGTTCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATCCAGGGCCTGGAACTGAAc < 1:38532/150‑1 (MQ=255) aaGTTCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATCCAGGGCCTGGAGCTGAACgcc > 2:441373/1‑150 (MQ=255) gTTCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATCCAGGGCCTGGAACTGAACgccgg < 1:1189095/150‑1 (MQ=255) ttCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCtt < 1:24207/95‑1 (MQ=255) ttCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCtt > 2:24207/1‑95 (MQ=255) aaCACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATCCAGGGCCTGGAACTGAACGCCGGCCGCTTCACCCAg < 1:975277/150‑1 (MQ=255) aaCACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGACAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCCTCACCGGCACCCTGATCACCTCCAAAGAGATCCAGGGCCTGGAACTGAACGCCGGCCGCTTCACCCAg < 1:143409/150‑1 (MQ=255) cGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATCCAg > 1:655266/1‑112 (MQ=255) cGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATCCAg < 2:655266/112‑1 (MQ=255) tGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATCCAGGGCCTGGAAc < 1:821951/117‑1 (MQ=255) tGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATCCAGGGCCTGGAAc > 2:821951/1‑117 (MQ=255) gAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCAc > 1:1273718/1‑75 (MQ=255) gAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCAc < 2:1273718/75‑1 (MQ=255) aGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATCCAGGGCCTGGAACTGAACGCCGGCCGCTTCACCCAGGAAGCGCGCAaga < 2:1357945/150‑1 (MQ=255) gTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATCCAGGGCCTGGAACTGAACGCCGGCCGCTTCACCCAGGAAGCGCGCAagag > 2:572991/1‑150 (MQ=255) ggTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATCCAGGGCCTGGAACTGAACGCCGGCCGCTTCACCCAGGAAGCGCGCAAgagcgcc < 2:270010/150‑1 (MQ=255) tgcAGCACTGGCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATCCAGGGCCTGGAACTGaa > 1:398832/3‑102 (MQ=255) tgcAGCACTGGCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATCCAGGGCCTGGAACTGaa < 2:398832/100‑1 (MQ=255) cAGCACTGGCAGTGCTGCAGTACGACGATGCACGTc < 2:695101/36‑1 (MQ=37) cAGCACTGGCAGTGCTGCAGTACGACGATGCACGTc > 1:695101/1‑36 (MQ=37) cAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATCCAGGGCCTGGAACTGAACGCCGGCCGCTTCACCCAGGAAGCGCGCAAGAGCGCCGAGCGCCGTGAtg < 2:568283/150‑1 (MQ=255) gCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATCCAGGGCCTGGAACTGAACGCCGGCCGCTTCACCCAGGAAGCGCGCAAGAGCGCCGAGCGCCGTGAtggt < 2:959718/150‑1 (MQ=255) cTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATCCAGGGCCTGGAACTGAACGCCGGCCGCTTCACCCAGGAAGCGCGCAAGAGCGCCGAGCGCCGTGAtggtggt < 1:441373/150‑1 (MQ=255) cTGCCAGTGCTGCAGTAAAACTATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATCCAGGGCCTGGAACTGAACGCCGGCCGCTTCACCCAGGAAGCGCGCAAGAGCGCCGAGCGCCGTGAtggtggt < 1:1141149/150‑1 (MQ=255) | GCGTCGACTTCTTCAAGCCGCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGTTCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATCCAGGGCCTGGAACTGAACGCCGGCCGCTTCACCCAGGAAGCGCGCAAGAGCGCCGAGCGCCGTGATGGTGGT > NC_002947/325018‑325307 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |