Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,638,706 T→G 9.5% L220R (CTG→CGG)  PP_3205 → fumarylacetoacetate hydrolase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,638,7060TG 9.5% 136.1 / 7.3 53L220R (CTG→CGG) PP_3205fumarylacetoacetate hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base T (24/24);  new base G (3/2);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01

ATGTCAGCCTGCGGGCCCTGGCACCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGC  >  NC_002947/3638559‑3638855
                                                                                                                                                   |                                                                                                                                                     
aTGTCAGCCTGCGGGCCCTGGCACCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGc                                                                                                                                                     <  1:939790/150‑1 (MQ=255)
            gggCCCTGGCACCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGa                                                                                                                                         >  2:1422728/1‑150 (MQ=255)
              gCCCTGGCACCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAAc                                                                                                                                       >  1:599731/1‑150 (MQ=255)
                 cTGGCACCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACGTGCAGCGGCAAGTGCAc                                                                                                                                             <  2:882765/141‑1 (MQ=255)
                 cTGGCACCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGAGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACGTGCAGCGGCAAGTGCAc                                                                                                                                             >  1:882765/1‑141 (MQ=255)
                  tGGCACCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCg                                                                                                                                   >  2:325032/1‑150 (MQ=255)
                   ggCACCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGa                                                                                                                                  <  1:974232/150‑1 (MQ=255)
                    gCACCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCAAGGCCTGGCGCGACGGCCAGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAg                                                                                                                                 <  2:241572/150‑1 (MQ=255)
                      aCCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCt                                                                                                                                                       <  2:869273/126‑1 (MQ=255)
                      aCCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCt                                                                                                                                                       >  1:869273/1‑126 (MQ=255)
                       ccGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACACCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTgg                                                                                                                              >  2:1213642/1‑150 (MQ=255)
                           gggAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCg                                                                                                                          <  2:457017/150‑1 (MQ=255)
                                      aCCGGCTTCGGCTTTCTCCAGACAAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCAt                                                                                                               <  2:330457/150‑1 (MQ=255)
                                       ccGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATcccatca                                                                                                              <  1:1119505/150‑5 (MQ=255)
                                          gcttcggcttTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGGGTGGCGCGACGGCTGGGGGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATggcg                                                                                                           <  1:531335/150‑1 (MQ=255)
                                                gcttTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGaa                                                                                                                                        >  2:1418496/1‑115 (MQ=255)
                                                gcttTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGaa                                                                                                                                        <  1:1418496/115‑1 (MQ=255)
                                                  tttCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATg                                                                                                   >  1:66766/1‑150 (MQ=255)
                                                  tttCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGCCGCCATg                                                                                                   <  1:826790/150‑1 (MQ=255)
                                                   ttCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAAAGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGc                                                                                                  <  2:575801/150‑1 (MQ=255)
                                                      tcCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGACCATCCCCATGGCGGCGCCGTGCACt                                                                                               >  1:778119/1‑150 (MQ=255)
                                                        cAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTc                                                                                             <  1:96879/150‑1 (MQ=255)
                                                         aGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCg                                                                                            >  2:920986/1‑150 (MQ=255)
                                                         aGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCg                                                                                            <  2:357076/150‑1 (MQ=255)
                                                                      cagcagTTTCGCGCTCGTTGCGGGCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGtt                                                                               <  2:442299/150‑1 (MQ=255)
                                                                            tttCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATCGc                                                                         <  1:1422728/150‑1 (MQ=255)
                                                                                   cccGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATgcg                                                                  >  2:195216/1‑150 (MQ=255)
                                                                                                 cccGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTgacgcgacg                                                    >  2:1385521/1‑150 (MQ=255)
                                                                                                   cGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCt                                                  >  1:919583/1‑150 (MQ=255)
                                                                                                   cGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCt                                                  >  2:835580/1‑150 (MQ=255)
                                                                                                    ggACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCtt                                                 <  2:599731/150‑1 (MQ=255)
                                                                                                            tCGGCGCGGCTTGGTGCGACGGCCAGGTGCACTTGCCGCTGCGCGTGCACTGGAAAGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGGCGCTTTAGTGCgg                                         >  2:418592/1‑150 (MQ=255)
                                                                                                            tCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCgg                                         >  2:403147/1‑150 (MQ=255)
                                                                                                            tCGGCGAGGCCTGGCGAGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTccacc                                                                                   >  1:335580/1‑108 (MQ=255)
                                                                                                            tCGGCGAGGCCTGGCGAGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTccacc                                                                                   <  2:335580/108‑1 (MQ=255)
                                                                                                               gCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCa                                      <  1:195216/150‑1 (MQ=255)
                                                                                                                    gCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTgacgcgac                                                     >  2:1266201/1‑130 (MQ=255)
                                                                                                                    gCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTgacgcgac                                                     <  1:1266201/130‑1 (MQ=255)
                                                                                                                    gCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTa                                 >  2:406484/1‑150 (MQ=255)
                                                                                                                                  ccGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCAc                   >  2:994739/1‑150 (MQ=255)
                                                                                                                                   cGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAg                                                                                 >  1:1343457/1‑87 (MQ=255)
                                                                                                                                   cGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAg                                                                                 <  2:1343457/87‑1 (MQ=255)
                                                                                                                                    gggTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGcc                                                                                                      <  2:768583/65‑1 (MQ=255)
                                                                                                                                    gggTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGcc                                                                                                      >  1:768583/1‑65 (MQ=255)
                                                                                                                                     ggTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCgt                <  1:835580/150‑1 (MQ=255)
                                                                                                                                     agTGCACGTGCAGCGGCAAGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCgt                >  2:1306168/2‑150 (MQ=255)
                                                                                                                                      gTGCACGTGCAGCGGCAAGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCgt                <  2:338134/149‑1 (MQ=255)
                                                                                                                                      gTGCACGTGCAGCGGCAAGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCgt                >  1:338134/1‑149 (MQ=255)
                                                                                                                                            ttGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAAc         <  1:1213642/150‑1 (MQ=255)
                                                                                                                                              gCCGCTGCACGTGCACTTGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTgacgcgac                                                     <  1:379930/104‑1 (MQ=255)
                                                                                                                                              gCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTgacgcgac                                                     >  2:379930/1‑104 (MQ=255)
                                                                                                                                                cGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGa                                               <  1:284192/108‑1 (MQ=255)
                                                                                                                                                cGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGa                                               >  2:284192/1‑108 (MQ=255)
                                                                                                                                                cGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACAAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCg     >  1:96494/1‑150 (MQ=255)
                                                                                                                                                  cTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAg   >  1:1253051/1‑150 (MQ=255)
                                                                                                                                                   tGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAgc  <  1:420327/150‑1 (MQ=255)
                                                                                                                                                   tGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAgc  <  1:994739/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
ATGTCAGCCTGCGGGCCCTGGCACCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGC  >  NC_002947/3638559‑3638855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑