Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,645,945 A→T 6.7% L32Q (CTG→CAG)  PP_3212 ← Rieske 2Fe‑2S family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,645,9450AT 6.7% 225.6 / 4.3 73L32Q (CTG→CAG) PP_3212Rieske 2Fe‑2S family protein
Reads supporting (aligned to +/- strand):  ref base A (33/34);  new base T (3/2);  total (36/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTCGTATTCCCAGCCGTGATACCCGCAGGCGATATTGCCCTCAGCACTGACGAAGCCTTTGGACAGTTTGGCGGTGCGGTGGCAGCAGCGGTCCCGCATTGCCACCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGGCGCAATACAGGTTGTTTGGTAACCAACATAATCAGGATTCCTCACAGCGTTGAGCTTGTTATGGTGTTCAG  >  NC_002947/3645796‑3646081
                                                                                                                                                     |                                                                                                                                        
aGTCGTATTCCCAGCCGTGATACCCGCAGGCGATATTGCCCTCAGCACTGACGAAGCCTTTGGACAGTTTGGCGGTGCGGTGGCAGCAGCGGTCCCGCATTGCCACCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCca                                                                                                                                          <  2:714702/150‑1 (MQ=255)
  tCGTATTCCCAGCCGTGATACCCGCAGGCGATATTGCCCTCAGCACTGACGAAGCCTTTGGACAGTTTGGCGGTGCGGTGGCAGCAGCGGTCCCGCATTGCCACCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCcagc                                                                                                                                        >  1:1138778/1‑150 (MQ=255)
      aTTCCCAGCCGTGATACCCGCAGGCGATATTGCCCTCAGCACTGACGAAGCCTTTGGACAGTTTGGCGGTGCGGTGGCAGCAGCGGTCCCGCATTGCCACCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAggg                                                                                                                                    >  2:192341/1‑150 (MQ=255)
       ttCCCAGCCGTGATACCCGCAGGCGATATTGCCCTCAGCACTGACGAAGCCTTTGGACAGTTTGGCGGTCCGGTGGCAGCAGCGGTCCCGCATTGCCACCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGt                                                                                                                                   <  1:1157581/150‑1 (MQ=255)
           cAGCCGTGATACCCGCAGGCGATATTGCCCTCAGCACTGACGAAGCCTTTGGACAGTTTGGCGGTGCGGTGGCAGCAGCGGTCCCGCATTGCCACCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAAt                                                                                                                               >  2:672928/1‑150 (MQ=255)
                 tGATACCCGCAGGCGATATTGCCCTCAGCACTGACGAAGCCTTTGGACAGTTTGGCGGTGCGGTGGCAGCAGCGGTCCCGCATTGCCACCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTg                                                                                                                         <  2:131373/150‑1 (MQ=255)
                      cccGCAGGCGATATTGCCCTCAGCACTGACGAAGCCTTTGGACAGTTTGGCGGTGCGGTGGCAGCAGCGGTCCCGCATTGCCACCGGGGTCCCATCGGGACGCTTCCACAACACAAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGcc                                                                                                                    >  1:1344549/1‑150 (MQ=255)
                       ccGCAGGCGATATTGCCCTCAGAACTGACGAAGCCTTTGGACAGTTTGGCGGTGCGGTGGCAGCAGCGGTCCCGCATTGCCACCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCTGCTGGGTGaa                                                                                                                                >  1:1102836/1‑137 (MQ=255)
                       ccGCAGGCGATATTGCCCTCAGAACTGACGAAGCCTTTGGACAGTTTGGCGGTGCGGTGGCAGCAGCGGTCCCGCATTGCCACCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCTGCTGGGTGaa                                                                                                                                <  2:1102836/137‑1 (MQ=255)
                            ggCGATATTGCCCTCAGCACTGACGAAGCCTTTGGACAGTTTGGCGGTGCGGTGGCAGCAGCGGTCCCGCATTGCCACCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTc                                                                                                              <  2:10360/150‑1 (MQ=255)
                                 tatTGCCCTCAGCACTGACGAAGCCTTTGGACAGTTTGGCGGTGCGGTGGCAGCAGCGGTCCCGCATTGCCACCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAggg                                                                                                         <  1:368972/150‑1 (MQ=255)
                                  atTGCCCTCAGCACTGACGAAGCCTTTGGACAGTTTGGCGGTGCGGTGGCAGCAGCGGTCCCGCATTGCCACCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGc                                                                                                        >  1:830353/1‑150 (MQ=255)
                                       ccTCAGCACTGACGAAGCCTTTGGACAGTTTGGCGGTGCGGTGGCAGCAGCGGTCCCGCATTGCCACCGGGGTACCTTGAAGCGCCGAGCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCTGTCTTCGAGGGCTGTCa                                                                                                   >  1:798469/1‑150 (MQ=255)
                                                tGACGAAGCCTTTGGACAGTTTGGCGGTGCGGTGGCAGAAGAGGTGCCGCATTACCACCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCa                                                                                          >  1:604811/1‑150 (MQ=255)
                                                  aCGAAGCCTTTGGACAGTTTGGCGGTGCGGTGGCAGCAGCGGTCCCGCATTGCCACCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAgg                                                                                        <  1:505170/150‑1 (MQ=255)
                                                    gAAGCCTTTGGACAGTTTGGCGGTGCGGTGGCAGCAGCGGTCCCGCATTGCCACCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGc                                                                                      >  1:22235/1‑150 (MQ=255)
                                                          tttGGACAGTTTGGCGGTGCGGTGGCAGCAGCGGTCCCGCATTGCTACCGTGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCa                                                                                >  2:201888/1‑150 (MQ=255)
                                                          tttGGACAGTTTGGCGGTGCGGTGGCAGCAGCGGTCCCGCATTGCCACCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGAATAccc                                                                                >  1:240075/1‑149 (MQ=255)
                                                             ggACAGTTTGGCGGTGCGGTGGCAGCAGCGGTCCCGCATTGCCACCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGaa                                                                             >  2:116283/1‑150 (MQ=255)
                                                               aCAGTTTGGCGGTGCGGTGGCAGCAGCGGTCCCGCATTGCCACCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAAc                                                                           >  1:321059/1‑150 (MQ=255)
                                                               aCAGTTTGGCGATGCGGTGGCAGCAGCGGTCCCGCATTGCCACCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAgg                                                                                               >  2:256182/1‑130 (MQ=255)
                                                               aCAGTTTGGCGATGCGGTGGCAGCAGCGGTCCCGCATTGCCACCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAgg                                                                                               <  1:256182/130‑1 (MQ=255)
                                                                 aGTTTGGCGGTGCGGTGGCAGCAGCGGTCCCGCATTGCCACCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACgg                                                                         <  1:192341/150‑1 (MQ=255)
                                                                  gTTTGGCGGTGCGGTGGCAGCAGCGGTCCCGCATTGCCACCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGgc                                                                        <  1:1192142/150‑1 (MQ=255)
                                                                   tttGGCGGTGCGGTGGCAGCAGCGGTCCCGCATTGCCACCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTc                                                                                                    >  2:189798/1‑121 (MQ=255)
                                                                   tttGGCGGTGCGGTGGCAGCAGCGGTCCCGCATTGCCACCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTc                                                                                                    <  1:189798/121‑1 (MQ=255)
                                                                        cggtgcggtgGCAGCAGCGGTCCCGCATTGCCACCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGGCGCAATa                                                                  <  2:1334099/150‑1 (MQ=255)
                                                                        cggtgcggtgGCAGCAGCGGTCCCGCATTGCCACCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGGCGCAATa                                                                  <  2:321059/150‑1 (MQ=255)
                                                                                tgGCAGCAGCGGTCCCGCATTGCCACCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGGCGCAATACAGgttgt                                                          >  2:236245/1‑150 (MQ=255)
                                                                                   cagcagCGGTCCCGCATTGCCACCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGGCGCAATACAGGTTGTTTg                                                       <  2:1101826/150‑1 (MQ=255)
                                                                                        gcGGTCCCGCATTGCCACCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGGCGCAATACAGGTTGTTTGGTAAc                                                  <  2:302503/150‑1 (MQ=255)
                                                                                           gTCCCGCATTGCCACCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGGCGCAATACAGGTTGTTTGGTAACCaa                                               <  2:22235/150‑1 (MQ=255)
                                                                                            tCCCGCATTGCCACCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGGCGCAATACAGGTTGTTTGGTAACCAAc                                              >  1:1224509/1‑150 (MQ=255)
                                                                                               cGCATTGCCACCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAggg                                                                                       >  1:173261/1‑106 (MQ=255)
                                                                                               cGCATTGCCACCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAggg                                                                                       <  2:173261/106‑1 (MQ=255)
                                                                                               cGCATTGCCACCGGGGTACCATCGGGACGCTGCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGGCGCAATACAGGTTGTTTGGTAACCAACATa                                           >  2:877067/1‑150 (MQ=255)
                                                                                                  aTTGCCACCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGGCGCAATACAGGTTGTTTGGTAACCAACATAATc                                        <  1:116283/150‑1 (MQ=255)
                                                                                                   cgggcACCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGGCGCAATACAGGTTGTTTGGTaa                                                   >  2:1153956/5‑138 (MQ=255)
                                                                                                   cgggcACCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGGCTTCGAGGGCTGTCATAGGGAGCAGCGCATACCAGAAACGGCGCAATACAGGTTGTTTGGTaa                                                   <  1:1153956/134‑1 (MQ=255)
                                                                                                    tGCCACCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGGCGCAATACAGGTTGTTTGGTAACCAACATAATCAg                                      <  2:875629/150‑1 (MQ=255)
                                                                                                     gCCACCGGGGTACCATCGGGACGCTTCAACAACTACTGTGGCACGCCCAGTAGGGTGAATGGCTTGGGGCCGTCTGCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGGCGCAATACAGGTTGTTTGGTAACCAACATAATCAgg                                     <  1:672928/150‑1 (MQ=255)
                                                                                                        aCCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTggg                                                                                                                       >  1:845295/1‑65 (MQ=255)
                                                                                                        aCCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTggg                                                                                                                       <  2:845295/65‑1 (MQ=255)
                                                                                                        aCCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGGCGCAATACAGGTTGTTTGGTAACCAACATAATCAGGAtt                                  >  2:1364551/1‑150 (MQ=255)
                                                                                                        aCCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGGCGCAATACAGGTTGTTTGGTAACCAACATAATCAGGAtt                                  >  2:1381800/1‑150 (MQ=255)
                                                                                                        aCCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCATCGGCGGCTTTCCTAGGGAGCAGGGCATACTACAAAAGGGCCAATACAGGTTGTTTGGTAACCAACATAATCAGGAtt                                  >  1:673074/1‑150 (MQ=255)
                                                                                                         ccGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGGCGCAATACAGGTTGTTTGGTAACCAACATAATCAGGATTc                                 <  1:201888/150‑1 (MQ=255)
                                                                                                           ggggTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGGCGCAATACAGGTTGTTTGGTAACCAACATAATCAGGATTCCt                               <  1:236245/150‑1 (MQ=255)
                                                                                                           ccggTACCATCGGGGCGCGTCCACAACGCCAGCACTTTACCCCGCAGGGTGAATGGCTTGGGCCCGTCTCCGAGGGCTGTCATAGCGAGCAGGGCATACCAGAGACGTCGCAATCCAGCCTGTTTGGCAACCAACATAATCAGGATTCCt                               <  2:629556/148‑1 (MQ=255)
                                                                                                              gTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGGCGCAATACAGGTTGTTTGGTAACCAACATAATCAGGATTCCTcac                            >  1:1347622/1‑150 (MQ=255)
                                                                                                                aCCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGGCGCAATACAGGTTGTTTGGTAACCAACATAATCAGGATTCCTCACAg                          <  2:1293763/150‑1 (MQ=255)
                                                                                                                      gggACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGGCGCAATACAGGTTGTTTGGTAACCAACATAATCAGGATTCCTCACAGCGTTGa                    >  2:643691/1‑150 (MQ=255)
                                                                                                                       ggACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTggg                                                                                                                       <  2:913241/50‑1 (MQ=255)
                                                                                                                       ggACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTggg                                                                                                                       >  1:913241/1‑50 (MQ=255)
                                                                                                                           gCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGGCGCAATACAGGTTGTTTGGTAACCAACATAATCAGGa                                    >  2:1216844/1‑129 (MQ=255)
                                                                                                                           gCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGGCGCAATACAGGTTGTTTGGTAACCAACATAATCAGGa                                    <  1:1216844/129‑1 (MQ=255)
                                                                                                                           gCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGGCGCAATACAGGTTGTTTGGTAACCAACATAATCAGGATTCCTCACAGCGTTGAGCTTg               >  2:1290151/1‑150 (MQ=255)
                                                                                                                           ctttCCACAACACAAGCGATTAAACCAACTGCGTAAACGGCTGGCTGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGGCGCAATACAGGTTGTTTGGTAACCAACATAATCAGGATTCCTCACAGCGTTGAGCTTg               <  2:13632/148‑1 (MQ=255)
                                                                                                                            cTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGGCGCAATACAGGTTGTTTGGTAACCAACATAATCAGGATTCCTCACAGCGTTGAGCTTGt              <  1:877067/150‑1 (MQ=255)
                                                                                                                             ttCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGACGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAATCGGCGCAATACAGGTTGTTTGGTAACCACCATATTCAGGACTCATCACAGCGTTGAGCTTGtt             <  2:798469/150‑1 (MQ=255)
                                                                                                                              tCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGGCGCAATACAGGTTGTTTGGATGCCAACATAATCAGGATTCCTCACAGCGTTGAGCTTGtag            >  1:13632/1‑148 (MQ=255)
                                                                                                                                 acaacaCCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGGCGCAATACAGGTTGTTTGGTAACCAACATAATCAGGATTCCTCACAGCGTTGAGCTTGTTATgg         >  2:852529/1‑150 (MQ=255)
                                                                                                                                  caacaCCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGGCGCAATACAGGTTGTTTGGTAACCAACATAATCAGGATTCCTCACAGCGTTGAGCTTGTTATGgt        <  1:643691/150‑1 (MQ=255)
                                                                                                                                   aacaCCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGGCGCAATACAGGTTGttt                                                        <  1:620611/101‑1 (MQ=255)
                                                                                                                                   aacaCCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGGCGCAATACAGGTTGttt                                                        >  2:620611/1‑101 (MQ=255)
                                                                                                                                     cacCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGGCGCAATACAGGTTGTTTGGTAACCAACATAATCAGGATTCCTCACAGCGTTGAGCTTGTTATGGTGtt     <  2:1344549/150‑1 (MQ=255)
                                                                                                                                        cAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGGCGCAATACAGGTTGTTTg                                                       <  1:249724/97‑1 (MQ=255)
                                                                                                                                        cAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGGCGCAATACAGGTTGTTTg                                                       >  2:249724/1‑97 (MQ=255)
                                                                                                                                        aagccattCACCCTTCTGGGTGAATCGCTTGGGGCCGTCTTTGTGGTCTGGGAGAGTGAGCAGGGCATACCAGAAACGGCGCAATACAGTGTGTTTTGTAACCAAGATAATCAGTATTCCTCACAGCGTTGAGCTTGTTATGTAGTTCAg  >  2:557405/7‑150 (MQ=255)
                                                                                                                                           ccattCACCCTGCTGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGgc                                                                        >  2:1054600/4‑77 (MQ=39)
                                                                                                                                           ccattCACCCTGCTGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGgc                                                                        <  1:1054600/74‑1 (MQ=39)
                                                                                                                                                   ccAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGgc                                                                        <  1:512315/69‑1 (MQ=255)
                                                                                                                                                   ccAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGgc                                                                        >  2:512315/1‑69 (MQ=255)
                                                                                                                                                   ccAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGGCGCAATa                                                                  >  2:600337/1‑75 (MQ=255)
                                                                                                                                                   ccAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGGCGCAATa                                                                  <  1:600337/75‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                        
AGTCGTATTCCCAGCCGTGATACCCGCAGGCGATATTGCCCTCAGCACTGACGAAGCCTTTGGACAGTTTGGCGGTGCGGTGGCAGCAGCGGTCCCGCATTGCCACCGGGGTACCATCGGGACGCTTCCACAACACCAGCGCTTCACCCAGCAGGGTGAATGGCTTGGGGCCGTCTTCGAGGGCTGTCATAGGGAGCAGGGCATACCAGAAACGGCGCAATACAGGTTGTTTGGTAACCAACATAATCAGGATTCCTCACAGCGTTGAGCTTGTTATGGTGTTCAG  >  NC_002947/3645796‑3646081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑