Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,659,949 C→G 6.4% intergenic (‑41/+34) PP_3223 ← / ← PP_3224 ABC transporter substrate‑binding protein/class II aldolase/adducin domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,659,9490CG 6.4% 190.7 / 4.6 63intergenic (‑41/+34)PP_3223/PP_3224ABC transporter substrate‑binding protein/class II aldolase/adducin domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (27/32);  new base G (2/2);  total (29/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.89e-01

AGAAGGCGATGTTGAGGGTCTTGCTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGACTGCTCATGCACCTTGA  >  NC_002947/3659800‑3660098
                                                                                                                                                     |                                                                                                                                                     
aGAAGGCGATGTTGAGGGTCTTGCTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGcc                                                                                                                                                       >  2:781703/1‑150 (MQ=255)
    ggCGATGTTGAGGGTCTTGCTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGtt                                                                                                                                                   >  2:494139/1‑150 (MQ=255)
    ggCGATGTTGAGGGTCTTGCTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGtt                                                                                                                                                   <  1:552363/150‑1 (MQ=255)
       gATGTTGAGGGTCTTGCTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGGGGTAAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTTTGTTGTTTGTGCCAGtttcc                                                                                                                                                >  2:1292169/1‑147 (MQ=255)
        aTGTTGAGGGTCGTGCTGGTGGAGGTGTGGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGcc                                                                                                                                               <  2:228833/150‑1 (MQ=255)
         tGTTGAGGGTCTTGCTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCg                                                                                                                                              <  1:494139/150‑1 (MQ=255)
          gTTGAGGGTCTTGCTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCgg                                                                                                                                             >  2:987175/1‑150 (MQ=255)
              aGGGTCTTGCTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATCAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACa                                                                                                                                         <  1:1150404/150‑1 (MQ=255)
                       cTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAgg                                                                                                                                >  1:396884/1‑150 (MQ=255)
                        tGGATGAGGTGCTTGCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGt                                                                                                                               <  2:325340/150‑1 (MQ=255)
                             gAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCtt                                                                                                                          <  1:1292169/150‑1 (MQ=255)
                             gAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCtt                                                                                                                          >  1:1418563/1‑150 (MQ=255)
                              aGGCGTTGTTGGCCGGCGGGCAGGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGAGACTGATTCTTCAATGAACGGGGCTTTCCTGTTCCGGCCAGTTTGCCGGAACAGGCAAACGGTGCCTTg                                                                                                                         <  2:10979/150‑1 (MQ=255)
                                gtgtTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGa                                                                                                                       <  2:1418563/150‑1 (MQ=255)
                                gtgtTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGAGTGCATTGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGa                                                                                                                       <  2:691806/150‑1 (MQ=255)
                                 tgttgtCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCGAACTGGCCGGAACAg                                                                                                                                        <  1:620571/132‑1 (MQ=255)
                                 tgttgtCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCGAACTGGCCGGAACAg                                                                                                                                        >  2:620571/1‑132 (MQ=255)
                                    tgtCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGagtca                                                                                                                   <  2:396884/150‑1 (MQ=255)
                                         gccggcGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCt                                                                                                              <  1:584709/150‑1 (MQ=255)
                                                  cAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGa                                                                                                     >  1:62778/1‑150 (MQ=255)
                                                  cAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGa                                                                                                     <  1:781703/150‑1 (MQ=255)
                                                   aaGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCCGTGATTGGGGCTCTCCTGTTCAGAGCAGTTGGGACGAGCATGGTAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAg                                                                                                    >  1:1385771/1‑150 (MQ=255)
                                                   aaGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGa                                                                                                                       <  1:860487/131‑1 (MQ=255)
                                                   aaGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGa                                                                                                                       >  2:860487/1‑131 (MQ=255)
                                                   aaGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAg                                                                                                    <  1:987175/150‑1 (MQ=255)
                                                   aaGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAg                                                                                                    >  2:935552/1‑150 (MQ=255)
                                                             gCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGa                                                                                          >  1:523663/1‑150 (MQ=255)
                                                              cccAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGAt                                                                                         <  2:62778/150‑1 (MQ=255)
                                                               ccagggccagggTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCGAACTGGCCGGAACAg                                                                                                                                        >  1:1274112/1‑102 (MQ=38)
                                                               ccagggccagggTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCGAACTGGCCGGAACAg                                                                                                                                        <  2:1274112/102‑1 (MQ=38)
                                                               ccagggccagggTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTACGGCCAGTTCGCCGGAACAGGCAACACGTGACTTAGCATCAGTCCTGGTAATCGAGGCCCTCGCGATc                                                                                        <  2:591235/150‑1 (MQ=255)
                                                                cagggccagggTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGGCAGTCCTGGTAAGCTATGCCCTCGCGATcc                                                                                       >  1:1002284/1‑150 (MQ=255)
                                                                 agggccagggTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTcc                                                                                                               <  1:298288/125‑1 (MQ=255)
                                                                 agggccagggTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTcc                                                                                                               >  2:298288/1‑125 (MQ=255)
                                                                              cGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATcg                                                                                       >  1:116370/1‑135 (MQ=255)
                                                                              cGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATcg                                                                                       <  2:116370/136‑2 (MQ=255)
                                                                                    cgAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATc                                                                   >  1:914385/1‑150 (MQ=255)
                                                                                       gCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCa                                                                >  2:1322539/1‑150 (MQ=255)
                                                                                         acttGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGTGGCTCTCCTTTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTTGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAAt                                                              <  2:1385771/148‑1 (MQ=255)
                                                                                         aGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAAt                                                              <  2:523663/150‑1 (MQ=255)
                                                                                           ttGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCAAATGc                                                            >  2:1071195/1‑150 (MQ=255)
                                                                                                gCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTg                                                       <  2:914385/150‑1 (MQ=255)
                                                                                                  aGTGGGGTCATGGGATTGATCCTTCCATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGCGACACTAAGAGCTCGATTGTCTCGCGATCCAACTGCCGAATCAGCGCATTCCAATGCCGTTGGa                                                     <  2:703202/150‑1 (MQ=255)
                                                                                                    tGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTgg                                                                                                            >  1:942110/1‑93 (MQ=255)
                                                                                                    tGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTgg                                                                                                            <  2:942110/93‑1 (MQ=255)
                                                                                                             gggATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTgg                                                                                                            <  2:422367/84‑1 (MQ=255)
                                                                                                             gggATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTgg                                                                                                            >  1:422367/1‑84 (MQ=255)
                                                                                                              ggATTGATCCTTCATTGAATGGGTCTCTCCTGTTCCGGCCAGTTCCCCGTAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTAGTAATCGCGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCt                                         <  2:129628/150‑1 (MQ=255)
                                                                                                                     cccTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATc                                  <  1:333798/149‑1 (MQ=255)
                                                                                                                       cTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCgg                                >  1:118719/1‑150 (MQ=255)
                                                                                                                        ttCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGcc                                                           <  2:568748/122‑1 (MQ=255)
                                                                                                                        ttCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGcc                                                           >  1:568748/1‑122 (MQ=255)
                                                                                                                        ttCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGa                               >  1:729680/1‑150 (MQ=255)
                                                                                                                         tCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGaa                              >  2:196984/1‑150 (MQ=255)
                                                                                                                                  ggggCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTg                     <  1:935552/150‑1 (MQ=255)
                                                                                                                                    ggCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCt                                                                                                              <  2:253400/59‑1 (MQ=255)
                                                                                                                                    ggCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCt                                                                                                              >  1:253400/1‑59 (MQ=255)
                                                                                                                                    ggCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGa                   <  1:376506/150‑1 (MQ=255)
                                                                                                                                         tcCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAgc                                                                        <  1:230439/92‑1 (MQ=255)
                                                                                                                                         tcCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAgc                                                                        >  2:230439/1‑92 (MQ=255)
                                                                                                                                         tcCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGACTGCt              >  1:852956/1‑150 (MQ=255)
                                                                                                                                              ttCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCTATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGACTGCTCATGc         >  1:729059/1‑150 (MQ=255)
                                                                                                                                                   gCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGACTGCTCATGCACCtt    <  2:852956/150‑1 (MQ=255)
                                                                                                                                                   gCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGACTGCTCATGCACCtt    >  2:1089428/1‑150 (MQ=255)
                                                                                                                                                     cAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGACTGCTCATGCACCTTGa  >  2:348149/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGAAGGCGATGTTGAGGGTCTTGCTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGACTGCTCATGCACCTTGA  >  NC_002947/3659800‑3660098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑