Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,686,484 A→G 13.5% Q283Q (CAA→CAG)  PP_3251 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,686,4840AG13.5% 123.6 / 10.8 52Q283Q (CAA→CAG) PP_3251hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (18/27);  new base G (2/5);  total (20/32)
Fisher's exact test for biased strand distribution p-value = 6.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.44e-01

GCGGCCACGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCATTACAGTCTCAT  >  NC_002947/3686336‑3686631
                                                                                                                                                    |                                                                                                                                                   
gCGGCCACGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAg                                                                                                                                                    <  2:516731/150‑1 (MQ=255)
gCGGCCACGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAg                                                                                                                                                    <  2:461694/150‑1 (MQ=255)
 cGGCCACGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAgg                                                                                                                                                   <  2:100763/150‑1 (MQ=255)
      aCGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGCCTTGCAGGTCGAAc                                                                                                                                              <  2:1062443/150‑1 (MQ=255)
        gcccgccTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTg                                                                                                                                            >  1:1277883/1‑150 (MQ=255)
         cccgccTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGCCTTGCAGGTCGAACTgg                                                                                                                                           <  2:1289460/150‑2 (MQ=255)
            cccTGGCCATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGAAGGCGATCTACGCCCGGGCCCGCTTAGGATACCCCCTGAATAACCGGTGTATCGCCACGCCAGACCTCATCCGCCAGTTCGCCTTGCAGGTCGAACTggcgg                                                                                                                                        <  2:519569/149‑5 (MQ=255)
              cTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCa                                                                                                                                      <  2:930450/150‑1 (MQ=255)
              cTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGACAGTTCGCCTTGCAGGTCGAACTggc                                                                                                                                          <  1:349353/146‑3 (MQ=255)
              cTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTATACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGACAGTTCGCCTTGCAGGTCGAACTggc                                                                                                                                          >  2:349353/1‑144 (MQ=255)
                    aTTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCtt                                                                                                                                <  2:1277883/150‑1 (MQ=255)
                       cAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGaa                                                                                                                             <  2:547291/150‑1 (MQ=255)
                        aGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTATGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAAc                                                                                                                            <  2:173847/150‑1 (MQ=255)
                        aGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCCCCGCAGTCCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAAc                                                                                                                            <  1:1243784/150‑1 (MQ=255)
                            gcTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCaa                                                                                                                        >  1:705466/1‑150 (MQ=255)
                            gcTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCaa                                                                                                                        >  1:774180/1‑150 (MQ=255)
                                aaCCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCgg                                                                                                                    >  1:695420/1‑150 (MQ=255)
                                  ccAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGt                                                                                                                  <  2:26006/150‑1 (MQ=255)
                                   cAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTc                                                                                                                 <  1:531384/150‑1 (MQ=255)
                                      cTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTAt                                                                                                              <  2:705466/150‑1 (MQ=255)
                                       tAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATg                                                                                                             <  2:339439/150‑1 (MQ=255)
                                        aaGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACAGGGGTATCGCAACGCCAGACCTCATCCGCCAGTTCGCCCTGCAAGGCGAACTGTTTGCAGCCCGTGACCGCAAGCGGGTCTATgg                                                                                                            <  2:318063/150‑1 (MQ=255)
                                          gCCCCTCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTCCCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGCACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGcc                                                                                                          <  2:448203/150‑1 (MQ=255)
                                                   aTGCTCCGCAAGCGTTCTGCGCCTGGGCGCGCTTAGGCTACAGCCTTAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGTTCTGCAAGGCGAACTGTTTGCAGCCCTGGAACGCAAGCGGGTCTATGGCCAATTGatgc                                                                                                 <  1:419963/150‑1 (MQ=255)
                                                   aTGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGccc                                                                                                                                  <  2:1160700/117‑1 (MQ=255)
                                                   aTGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGccc                                                                                                                                  >  1:1160700/1‑117 (MQ=255)
                                                      aTCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAAc                                                                                                                            >  2:648546/1‑120 (MQ=255)
                                                      aTCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAAc                                                                                                                            <  1:648546/120‑1 (MQ=255)
                                                         cGCAGGCGTTCTACGCCTGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCg                                                                                           >  2:730353/1‑150 (MQ=255)
                                                              gCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCAt                                                                                      <  2:367939/150‑1 (MQ=255)
                                                                gTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGCCTTGCAGGTCGAACTgg                                                                                                                                           <  2:504674/95‑2 (MQ=38)
                                                                gTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGCCTTGCAGGTCGAACTgg                                                                                                                                           >  1:504674/1‑94 (MQ=38)
                                                                    tACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTgccgc                                                                                <  1:289746/150‑1 (MQ=255)
                                                                    tACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGAAGGACGGTCATTGAACTCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTgccgc                                                                                >  2:1118913/1‑150 (MQ=255)
                                                                         cAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTgca                                                                           <  2:695420/150‑1 (MQ=255)
                                                                            ggCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTgcagca                                                                        >  2:324956/1‑150 (MQ=255)
                                                                                         tACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGatcaat                                                           >  2:394357/1‑150 (MQ=255)
                                                                                         tACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGatcaat                                                           <  2:577802/150‑1 (MQ=255)
                                                                                             gCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATcaatcaat                                                       <  1:394357/150‑1 (MQ=255)
                                                                                              ccTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAAtg                                                      >  2:643812/1‑150 (MQ=255)
                                                                                               cTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGaa                                                                                                                                               <  1:517216/60‑1 (MQ=255)
                                                                                               cTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGaa                                                                                                                                               >  2:517216/1‑60 (MQ=255)
                                                                                               cTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAAtgg                                                     <  2:774180/150‑1 (MQ=255)
                                                                                                                 gccacgccaGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTCTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGcc                                   >  2:200315/1‑150 (MQ=255)
                                                                                                                      gccaGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGcc                              >  2:1150969/1‑150 (MQ=255)
                                                                                                                       tcataCCTCATCCGCCAGTTCGACCTCCAAGCCGAACTGTTTGCAGCTCTTGAACGCAAGCGGGTCTATGGCAAAATGATGCATGCCGGCCATGGCCGCCTGCAGCAGCGCACGATCAATCATTGCCTGGCCGAACCCGGCGCCATGacc                             <  2:619052/146‑1 (MQ=255)
                                                                                                                               cATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGt                     >  2:704236/1‑150 (MQ=255)
                                                                                                                                aTCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGtt                    >  2:613697/1‑150 (MQ=255)
                                                                                                                                 tCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTg                   >  1:1108413/1‑150 (MQ=255)
                                                                                                                                             aCCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCATTACAGt       >  2:1322602/1‑150 (MQ=255)
                                                                                                                                                  cAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCATTACAGTCTCAt  <  2:1108413/150‑1 (MQ=255)
                                                                                                                                                  cAAGGCGAACTGTTTGCAGCCCTTGAACCCCAGTAACTCTGTGGCCAATTGATGCATGCCGACCATTGCCGCCTACAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGTCGCCATGCCCGCATTCGTTGCCGCATTACAGTCTCAt  <  2:474339/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCGGCCACGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCATTACAGTCTCAT  >  NC_002947/3686336‑3686631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑