Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,716,176 G→C 9.2% T52T (ACC→ACG) ‡ paaI ← hydroxyphenylacetyl‑CoA thioesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,716,1760GC 9.2% 129.4 / 5.3 44T52T (ACC→ACG) ‡paaIhydroxyphenylacetyl‑CoA thioesterase
Reads supporting (aligned to +/- strand):  ref base G (17/22);  new base C (2/2);  total (19/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.37e-01

TCGCTGGCGTGGGCGGTGAGCACGTCATCGCGCAGCGCCGGGGCCAGGTAGTCGATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTCGCGGGCGTACATGGCGTCGGCACAGGCTTGTGCCAGT  >  NC_002947/3716027‑3716317
                                                                                                                                                     |                                                                                                                                             
tCGCTGGCGTGGGCGGTGAGCACGTCATCGCGCAGCGCCGGGGCCTGGTAGTCGATTGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAg                                                                                                                                               <  1:1163956/150‑1 (MQ=255)
tCGCTGGCGTGGGCGGTGAGCACGTCATCGCGCAGCGCCGGGGCCAGGTAGTCGATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAg                                                                                                                                               <  1:927210/150‑1 (MQ=255)
  gCTGGCGTGGGCGGTGAGCACGTCATCGCGCAGCGCCGGGGCCAGGTAGTCGATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGt                                                                                                                                             <  2:1186786/150‑1 (MQ=255)
          gggCGGTGAGCACGTCATCGCGCAGCGCCGGGGCCAGGTAGTCGATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGcc                                                                                                                                     <  1:136618/150‑1 (MQ=255)
          gggCGGTGAGCACGTCATCGCGCAGCGCCGGGGCCAGGTAGTCGATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCACCTGCCATggc                                                                                                                                     <  1:847837/150‑3 (MQ=255)
              ggTGAGCACGTCATCGCGCAGCGCCGGGGCCAGGTAGTCGATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCATGTGCCATGCCCCTg                                                                                                                                 <  2:877327/150‑1 (MQ=255)
                  aGCACGTCATCGCGCAGCGCCGGGGCCGGGTAGTCGATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATc                                                                                                                             <  2:1227504/150‑1 (MQ=255)
                       gtaaTCGCGCAGCGCCGGGGCCAGGTAGTCGATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTc                                                                                                                        >  1:416309/4‑150 (MQ=255)
                       gTCATCGCGCAGCGCCGGGGCCAGGTAGTCGATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTc                                                                                                                        <  2:500630/150‑1 (MQ=255)
                                             aGGTAGTCGATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGTAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAgg                                                                                                  >  2:300177/1‑150 (MQ=255)
                                              ggTAGTCGATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGc                                                                                                 <  1:1328774/150‑1 (MQ=255)
                                               gTAGTCGATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTACCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCt                                                                                                >  2:407997/1‑150 (MQ=255)
                                                     gATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTggcccg                                                                                          >  1:494691/1‑150 (MQ=255)
                                                     gATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTggcccg                                                                                          >  2:869905/1‑150 (MQ=255)
                                                               cccAGCGCCACCGTGGCCTGGTCGTAGCTATTGTAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCgggccgggcca                                                                                <  1:300177/150‑2 (MQ=255)
                                                               cccAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCgggccgggcca                                                                                <  2:416309/150‑2 (MQ=255)
                                                                 cAGCGCCACCGTGGCCTGGTCGTAGCTATTGTAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGc                                                                              >  2:68998/1‑150 (MQ=255)
                                                                         ccGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCcagca                                                                      <  1:559473/150‑1 (MQ=255)
                                                                            tGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGc                                                                   >  1:1402145/1‑150 (MQ=255)
                                                                                 tGGTCGTAGCTATTGCAGGCGAAGGCGGACGCCTAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATa                                                              <  2:1177522/150‑1 (MQ=255)
                                                                                       tAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGt                                                                                                                                             <  1:231999/65‑1 (MQ=255)
                                                                                       tAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGt                                                                                                                                             >  2:231999/1‑65 (MQ=255)
                                                                                           tATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCt                                                    >  2:1265151/1‑150 (MQ=255)
                                                                                                   gcgaaggcgaaCGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCca                                                                         <  2:541737/121‑1 (MQ=255)
                                                                                                   gcgaaggcgaaCGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCca                                                                         >  1:541737/1‑121 (MQ=255)
                                                                                                     gaaggcgaaCGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCgg                                          <  1:869905/150‑1 (MQ=255)
                                                                                                           gaaCGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGt                                                                                                                         <  2:390776/65‑1 (MQ=255)
                                                                                                           gaaCGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGt                                                                                                                         >  1:390776/1‑65 (MQ=255)
                                                                                                           gaaCGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTcgcg                                    <  2:494691/150‑1 (MQ=255)
                                                                                                            aaCGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCACGGCCCGGGCCGGCATCCAGCAGGCTGATCCCCAGGCACTGGGTGGCCGGCTcgctg                                   <  2:764847/150‑3 (MQ=255)
                                                                                                             aCGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTACCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTCGCggg                                  <  1:407997/150‑1 (MQ=255)
                                                                                                             aCGCCGAAGCGGCCAACGCGAACAGGAAACCGCCATGGTAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTCGCggg                                  <  1:1068933/150‑1 (MQ=255)
                                                                                                               gCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTCGCGGGCg                                >  1:145051/1‑150 (MQ=255)
                                                                                                                ccGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAg                                                                                                   >  2:382902/1‑82 (MQ=255)
                                                                                                                ccGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAg                                                                                                   <  1:382902/82‑1 (MQ=255)
                                                                                                                           aaCGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTCGCGGGCGTACATGGCGTCg                    >  1:729951/1‑150 (MQ=255)
                                                                                                                                  aCAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTCGCGGGCGTACATGGCGTCGGCACAgg             <  1:1317653/150‑1 (MQ=255)
                                                                                                                                      gAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTCGCGGGCGTACATGGCGTCGGCACAGGCTtg         >  2:851529/1‑150 (MQ=255)
                                                                                                                                      gAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTCGCGGGCGTACATGGCGTCGGCACAGGCTtg         >  1:1050296/1‑150 (MQ=255)
                                                                                                                                       aaaCCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTCGCGGGCGTACATGGCGTCGGCACAGGCTtgt        <  1:851529/150‑1 (MQ=255)
                                                                                                                                       aaaCCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTCGCGGGCGTACATGGCGTCGGCACAGGCTtgt        <  1:68998/150‑1 (MQ=255)
                                                                                                                                          ccGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTCGCGGGCGTACATGGCGTCGGCACAGGCTTGTGcc     >  2:869987/1‑150 (MQ=255)
                                                                                                                                           gggcATGGCACCTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCcagca                                                                      >  1:610798/4‑84 (MQ=255)
                                                                                                                                           gggcATGGCACCTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCcagca                                                                      <  2:610798/81‑1 (MQ=255)
                                                                                                                                           cGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGTCGGCCGGGTCTCGGGCGTACATGGCGTCGGCACAGGCTTGTGCCa    <  1:328021/150‑1 (MQ=255)
                                                                                                                                             gcATGGCACCTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTCGCGGGCGTACATGGCGTCGGCACAGGCTTGTGCCAGt  >  1:1338726/2‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                             
TCGCTGGCGTGGGCGGTGAGCACGTCATCGCGCAGCGCCGGGGCCAGGTAGTCGATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTCGCGGGCGTACATGGCGTCGGCACAGGCTTGTGCCAGT  >  NC_002947/3716027‑3716317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑