Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,716,177 G→C 9.1% T52S (ACC→AGC) ‡ paaI ← hydroxyphenylacetyl‑CoA thioesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,716,1770GC 9.1% 130.2 / 5.2 44T52S (ACC→AGC) ‡paaIhydroxyphenylacetyl‑CoA thioesterase
Reads supporting (aligned to +/- strand):  ref base G (17/23);  new base C (2/2);  total (19/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.77e-01

GCTGGCGTGGGCGGTGAGCACGTCATCGCGCAGCGCCGGGGCCAGGTAGTCGATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTCGCGGGCGTACATGGCGTCGGCACAGGCTTGTGCCAGTTGCTCTTCA  >  NC_002947/3716029‑3716326
                                                                                                                                                    |                                                                                                                                                     
gCTGGCGTGGGCGGTGAGCACGTCATCGCGCAGCGCCGGGGCCAGGTAGTCGATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGt                                                                                                                                                      <  2:1186786/150‑1 (MQ=255)
        gggCGGTGAGCACGTCATCGCGCAGCGCCGGGGCCAGGTAGTCGATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGcc                                                                                                                                              <  1:136618/150‑1 (MQ=255)
        gggCGGTGAGCACGTCATCGCGCAGCGCCGGGGCCAGGTAGTCGATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCACCTGCCATggc                                                                                                                                              <  1:847837/150‑3 (MQ=255)
            ggTGAGCACGTCATCGCGCAGCGCCGGGGCCAGGTAGTCGATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCATGTGCCATGCCCCTg                                                                                                                                          <  2:877327/150‑1 (MQ=255)
                aGCACGTCATCGCGCAGCGCCGGGGCCGGGTAGTCGATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATc                                                                                                                                      <  2:1227504/150‑1 (MQ=255)
                     gtaaTCGCGCAGCGCCGGGGCCAGGTAGTCGATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTc                                                                                                                                 >  1:416309/4‑150 (MQ=255)
                     gTCATCGCGCAGCGCCGGGGCCAGGTAGTCGATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTc                                                                                                                                 <  2:500630/150‑1 (MQ=255)
                                           aGGTAGTCGATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGTAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAgg                                                                                                           >  2:300177/1‑150 (MQ=255)
                                            ggTAGTCGATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGc                                                                                                          <  1:1328774/150‑1 (MQ=255)
                                             gTAGTCGATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTACCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCt                                                                                                         >  2:407997/1‑150 (MQ=255)
                                                   gATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTggcccg                                                                                                   >  1:494691/1‑150 (MQ=255)
                                                   gATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTggcccg                                                                                                   >  2:869905/1‑150 (MQ=255)
                                                             cccAGCGCCACCGTGGCCTGGTCGTAGCTATTGTAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCgggccgggcca                                                                                         <  1:300177/150‑2 (MQ=255)
                                                             cccAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCgggccgggcca                                                                                         <  2:416309/150‑2 (MQ=255)
                                                               cAGCGCCACCGTGGCCTGGTCGTAGCTATTGTAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGc                                                                                       >  2:68998/1‑150 (MQ=255)
                                                                       ccGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCcagca                                                                               <  1:559473/150‑1 (MQ=255)
                                                                          tGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGc                                                                            >  1:1402145/1‑150 (MQ=255)
                                                                               tGGTCGTAGCTATTGCAGGCGAAGGCGGACGCCTAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATa                                                                       <  2:1177522/150‑1 (MQ=255)
                                                                                     tAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGt                                                                                                                                                      <  1:231999/65‑1 (MQ=255)
                                                                                     tAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGt                                                                                                                                                      >  2:231999/1‑65 (MQ=255)
                                                                                         tATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCt                                                             >  2:1265151/1‑150 (MQ=255)
                                                                                                 gcgaaggcgaaCGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCca                                                                                  <  2:541737/121‑1 (MQ=255)
                                                                                                 gcgaaggcgaaCGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCca                                                                                  >  1:541737/1‑121 (MQ=255)
                                                                                                   gaaggcgaaCGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCgg                                                   <  1:869905/150‑1 (MQ=255)
                                                                                                         gaaCGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGt                                                                                                                                  <  2:390776/65‑1 (MQ=255)
                                                                                                         gaaCGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGt                                                                                                                                  >  1:390776/1‑65 (MQ=255)
                                                                                                         gaaCGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTcgcg                                             <  2:494691/150‑1 (MQ=255)
                                                                                                          aaCGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCACGGCCCGGGCCGGCATCCAGCAGGCTGATCCCCAGGCACTGGGTGGCCGGCTcgctg                                            <  2:764847/150‑3 (MQ=255)
                                                                                                           aCGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTACCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTCGCggg                                           <  1:407997/150‑1 (MQ=255)
                                                                                                           aCGCCGAAGCGGCCAACGCGAACAGGAAACCGCCATGGTAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTCGCggg                                           <  1:1068933/150‑1 (MQ=255)
                                                                                                             gCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTCGCGGGCg                                         >  1:145051/1‑150 (MQ=255)
                                                                                                              ccGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAg                                                                                                            >  2:382902/1‑82 (MQ=255)
                                                                                                              ccGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAg                                                                                                            <  1:382902/82‑1 (MQ=255)
                                                                                                                         aaCGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTCGCGGGCGTACATGGCGTCg                             >  1:729951/1‑150 (MQ=255)
                                                                                                                                aCAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTCGCGGGCGTACATGGCGTCGGCACAgg                      <  1:1317653/150‑1 (MQ=255)
                                                                                                                                    gAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTCGCGGGCGTACATGGCGTCGGCACAGGCTtg                  >  2:851529/1‑150 (MQ=255)
                                                                                                                                    gAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTCGCGGGCGTACATGGCGTCGGCACAGGCTtg                  >  1:1050296/1‑150 (MQ=255)
                                                                                                                                     aaaCCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTCGCGGGCGTACATGGCGTCGGCACAGGCTtgt                 <  1:851529/150‑1 (MQ=255)
                                                                                                                                     aaaCCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTCGCGGGCGTACATGGCGTCGGCACAGGCTtgt                 <  1:68998/150‑1 (MQ=255)
                                                                                                                                        ccGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTCGCGGGCGTACATGGCGTCGGCACAGGCTTGTGcc              >  2:869987/1‑150 (MQ=255)
                                                                                                                                         gggcATGGCACCTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCcagca                                                                               >  1:610798/4‑84 (MQ=255)
                                                                                                                                         gggcATGGCACCTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCcagca                                                                               <  2:610798/81‑1 (MQ=255)
                                                                                                                                         cGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGTCGGCCGGGTCTCGGGCGTACATGGCGTCGGCACAGGCTTGTGCCa             <  1:328021/150‑1 (MQ=255)
                                                                                                                                           gcATGGCACCTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTCGCGGGCGTACATGGCGTCGGCACAGGCTTGTGCCAGt           >  1:1338726/2‑150 (MQ=255)
                                                                                                                                                    gTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTCGCGGGCGTACATGGCGTCGGCACAGGCTTGTGCCAGTTGCTCTTCa  <  2:1402145/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GCTGGCGTGGGCGGTGAGCACGTCATCGCGCAGCGCCGGGGCCAGGTAGTCGATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTCGCGGGCGTACATGGCGTCGGCACAGGCTTGTGCCAGTTGCTCTTCA  >  NC_002947/3716029‑3716326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑