Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,726,458 T→G 10.0% F356L (TTT→TTG)  PP_3291 → metallo‑beta‑lactamase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,726,4580TG10.0% 135.7 / 4.8 54F356L (TTT→TTG) PP_3291metallo‑beta‑lactamase family protein
Reads supporting (aligned to +/- strand):  ref base T (23/25);  new base G (4/2);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGACGGTCGATGAGTCCAGGCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCTGATGAAATCATGGA  >  NC_002947/3726309‑3726598
                                                                                                                                                     |                                                                                                                                            
cGGACGGTCGATGAGTCCAGGCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCttt                                                                                                                                              >  1:1394283/1‑150 (MQ=255)
          aTGAGTCCAGGCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCgggg                                                                                                                                    <  2:1394283/150‑1 (MQ=255)
              gTCCAGGCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCCCCGGCTTGAAAGCCggagaac                                                                                                                                 <  1:943407/149‑6 (MQ=255)
              gTCCAGGCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCCCCGGCTTGAAAGCCggagaac                                                                                                                                 >  2:943407/1‑144 (MQ=255)
                 cAGGCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCggg                                                                                                                                     >  1:45160/1‑142 (MQ=255)
                 cAGGCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCggg                                                                                                                                     <  2:45160/142‑1 (MQ=255)
                 cAGGCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGAcgcg                                                                                                                             >  2:915633/1‑150 (MQ=255)
                          ggACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGa                                                                                                                    <  1:634877/150‑1 (MQ=255)
                          cggcacacTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGa                                                                                                                    >  2:239562/8‑150 (MQ=255)
                                gCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATAtcgt                                                                                                              >  2:215663/1‑150 (MQ=255)
                                     gcgAGCCCGCAGTGATCATCGCCGAACTGTGCAGGGCGACCGGCGGAGGGGTGCTTCACCAACTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCgg                                                                                                         <  1:533/150‑1 (MQ=255)
                                         gCCCGCAGTGCTCATCGCCGCTAGCGGCATGTCGACCGGCGGACGGGTGCTTCACCACCTGAAGGGACTTTCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGgcgc                                                                                                     <  1:449549/150‑1 (MQ=255)
                                         gCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGgcgc                                                                                                     >  1:527650/1‑150 (MQ=255)
                                            cGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGGCGCGGGGTGCCGATATCGTCGCGGGCGCCca                                                                                                  >  1:1164871/1‑150 (MQ=255)
                                             gCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCgggg                                                                                                                                    >  2:454149/1‑115 (MQ=255)
                                             gCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCgggg                                                                                                                                    <  1:454149/115‑1 (MQ=255)
                                             gCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTGAAAGCCggagaa                                                                                                                                  <  2:1163994/117‑5 (MQ=255)
                                             gCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTGAAAGCCggagaa                                                                                                                                  >  1:1163994/1‑113 (MQ=255)
                                              ccgTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCcaca                                                                                                <  1:915633/148‑1 (MQ=255)
                                                   atcatcGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGtgtg                                                                                           <  2:520470/150‑1 (MQ=255)
                                                      atcGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGc                                                                                                                       <  1:298339/119‑1 (MQ=255)
                                                      atcGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGc                                                                                                                       >  2:298339/1‑119 (MQ=255)
                                                      atcGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCgg                                                                                        <  1:239562/150‑1 (MQ=255)
                                                                               gcggACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATcc                                                               <  1:1127951/150‑1 (MQ=255)
                                                                               gcggACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATcc                                                               <  1:215663/150‑1 (MQ=255)
                                                                                  gACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTg                                                            <  1:129056/150‑1 (MQ=255)
                                                                                       gTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGCTATCGTCGCGGGCGCCCCCAGTGTGCGGTTGCACGTCGAGGATGTGCCTATCCGTGCACAg                                                       >  1:871/1‑150 (MQ=255)
                                                                                        tGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCACCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAgg                                                      >  1:1154922/1‑150 (MQ=255)
                                                                                          cTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTg                                                    >  2:524346/1‑150 (MQ=255)
                                                                                           ttCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAg                                                       <  1:1321702/146‑1 (MQ=255)
                                                                                           ttCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAg                                                       >  2:1321702/1‑146 (MQ=255)
                                                                                             caccacCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCAt                                                 >  2:465280/1‑150 (MQ=255)
                                                                                              accaccTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGTACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATg                                                >  2:691283/1‑150 (MQ=255)
                                                                                              accaccTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATg                                                >  1:6885/1‑150 (MQ=255)
                                                                                                  ccTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGAt                                            <  1:60084/150‑1 (MQ=255)
                                                                                                     gAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGga                                         <  2:527650/150‑1 (MQ=255)
                                                                                                     gAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGga                                         >  1:403657/1‑150 (MQ=255)
                                                                                                           aCTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGc                                                                     <  1:405969/116‑1 (MQ=255)
                                                                                                           aCTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGc                                                                     >  2:405969/1‑116 (MQ=255)
                                                                                                           aCTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCt                                   >  1:1144416/1‑150 (MQ=255)
                                                                                                               gCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCATCACCGTGCACAGGTGCATGCGATGGAGAATCTTTCt                               <  1:691283/150‑1 (MQ=255)
                                                                                                                 cccAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCCCAGGTGCATGCGATGGAGAATCTTTCTgc                             >  2:1240490/1‑150 (MQ=255)
                                                                                                                 cccAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGAACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTgc                             >  2:399722/1‑150 (MQ=255)
                                                                                                                  ccAAACCCGCGCAACAGCAGTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCAgcg                            >  1:916565/1‑150 (MQ=255)
                                                                                                                        ccGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGcc                      >  1:861802/1‑150 (MQ=255)
                                                                                                                             cAACAGCATTCTGTTCTCCGGCTTTCAAGCCGATGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGc                 <  2:466837/150‑1 (MQ=255)
                                                                                                                                 aGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCtgat             <  2:6885/150‑1 (MQ=255)
                                                                                                                                     ttCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCTGATGaaa         <  1:368001/150‑1 (MQ=255)
                                                                                                                                        tGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCTGATGAAATCa      >  2:1030360/1‑150 (MQ=255)
                                                                                                                                         gTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCTGATGAAATCAt     <  2:974395/150‑1 (MQ=255)
                                                                                                                                         gTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGCATGTGCCTATCCGTGCACAGGTGCATGCCATGGAGAATCTTTCTGCGCACGCCGATGCTGATGAAATCAt     <  2:1164871/150‑1 (MQ=255)
                                                                                                                                          ttCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCTGATGAAATCATg    <  2:1144416/150‑1 (MQ=255)
                                                                                                                                          ttCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCTGATGAAATCATg    <  2:887273/150‑1 (MQ=255)
                                                                                                                                           cccccGGCTTGAAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCTGATGAAATCATgg   >  1:751032/4‑150 (MQ=255)
                                                                                                                                            ccccGGCTTGAAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCTGATGAAATCATGGa  >  1:718768/3‑150 (MQ=255)
                                                                                                                                                   tttCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCt                                   <  2:1275946/110‑1 (MQ=255)
                                                                                                                                                   tttCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCt                                   >  1:1275946/1‑110 (MQ=255)
                                                                                                                                                     |                                                                                                                                            
CGGACGGTCGATGAGTCCAGGCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCTGATGAAATCATGGA  >  NC_002947/3726309‑3726598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑