Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,726,459 C→A 10.2% Q357K (CAA→AAA)  PP_3291 → metallo‑beta‑lactamase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,726,4590CA10.2% 164.0 / 4.0 56Q357K (CAA→AAA) PP_3291metallo‑beta‑lactamase family protein
Reads supporting (aligned to +/- strand):  ref base C (24/26);  new base A (4/2);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

ATGAGTCCAGGCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCTGATGAAATCATGGAATGGTTGCGC  >  NC_002947/3726319‑3726608
                                                                                                                                            |                                                                                                                                                     
aTGAGTCCAGGCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCgggg                                                                                                                                              <  2:1394283/150‑1 (MQ=255)
    gTCCAGGCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCCCCGGCTTGAAAGCCggagaac                                                                                                                                           <  1:943407/149‑6 (MQ=255)
    gTCCAGGCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCCCCGGCTTGAAAGCCggagaac                                                                                                                                           >  2:943407/1‑144 (MQ=255)
       cAGGCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCggg                                                                                                                                               >  1:45160/1‑142 (MQ=255)
       cAGGCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCggg                                                                                                                                               <  2:45160/142‑1 (MQ=255)
       cAGGCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGAcgcg                                                                                                                                       >  2:915633/1‑150 (MQ=255)
                ggACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGa                                                                                                                              <  1:634877/150‑1 (MQ=255)
                cggcacacTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGa                                                                                                                              >  2:239562/8‑150 (MQ=255)
                      gCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATAtcgt                                                                                                                        >  2:215663/1‑150 (MQ=255)
                           gcgAGCCCGCAGTGATCATCGCCGAACTGTGCAGGGCGACCGGCGGAGGGGTGCTTCACCAACTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCgg                                                                                                                   <  1:533/150‑1 (MQ=255)
                               gCCCGCAGTGCTCATCGCCGCTAGCGGCATGTCGACCGGCGGACGGGTGCTTCACCACCTGAAGGGACTTTCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGgcgc                                                                                                               <  1:449549/150‑1 (MQ=255)
                               gCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGgcgc                                                                                                               >  1:527650/1‑150 (MQ=255)
                                  cGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGGCGCGGGGTGCCGATATCGTCGCGGGCGCCca                                                                                                            >  1:1164871/1‑150 (MQ=255)
                                   gCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCgggg                                                                                                                                              >  2:454149/1‑115 (MQ=255)
                                   gCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCgggg                                                                                                                                              <  1:454149/115‑1 (MQ=255)
                                   gCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTGAAAGCCggagaa                                                                                                                                            <  2:1163994/117‑5 (MQ=255)
                                   gCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTGAAAGCCggagaa                                                                                                                                            >  1:1163994/1‑113 (MQ=255)
                                    ccgTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCcaca                                                                                                          <  1:915633/148‑1 (MQ=255)
                                         atcatcGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGtgtg                                                                                                     <  2:520470/150‑1 (MQ=255)
                                            atcGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGc                                                                                                                                 <  1:298339/119‑1 (MQ=255)
                                            atcGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGc                                                                                                                                 >  2:298339/1‑119 (MQ=255)
                                            atcGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCgg                                                                                                  <  1:239562/150‑1 (MQ=255)
                                                                     gcggACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATcc                                                                         <  1:1127951/150‑1 (MQ=255)
                                                                     gcggACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATcc                                                                         <  1:215663/150‑1 (MQ=255)
                                                                        gACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTg                                                                      <  1:129056/150‑1 (MQ=255)
                                                                             gTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGCTATCGTCGCGGGCGCCCCCAGTGTGCGGTTGCACGTCGAGGATGTGCCTATCCGTGCACAg                                                                 >  1:871/1‑150 (MQ=255)
                                                                              tGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCACCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAgg                                                                >  1:1154922/1‑150 (MQ=255)
                                                                                cTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTg                                                              >  2:524346/1‑150 (MQ=255)
                                                                                 ttCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAg                                                                 <  1:1321702/146‑1 (MQ=255)
                                                                                 ttCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAg                                                                 >  2:1321702/1‑146 (MQ=255)
                                                                                   caccacCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCAt                                                           >  2:465280/1‑150 (MQ=255)
                                                                                    accaccTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGTACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATg                                                          >  2:691283/1‑150 (MQ=255)
                                                                                    accaccTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATg                                                          >  1:6885/1‑150 (MQ=255)
                                                                                        ccTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGAt                                                      <  1:60084/150‑1 (MQ=255)
                                                                                           gAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGga                                                   <  2:527650/150‑1 (MQ=255)
                                                                                           gAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGga                                                   >  1:403657/1‑150 (MQ=255)
                                                                                                 aCTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGc                                                                               <  1:405969/116‑1 (MQ=255)
                                                                                                 aCTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGc                                                                               >  2:405969/1‑116 (MQ=255)
                                                                                                 aCTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCt                                             >  1:1144416/1‑150 (MQ=255)
                                                                                                     gCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCATCACCGTGCACAGGTGCATGCGATGGAGAATCTTTCt                                         <  1:691283/150‑1 (MQ=255)
                                                                                                       cccAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCCCAGGTGCATGCGATGGAGAATCTTTCTgc                                       >  2:1240490/1‑150 (MQ=255)
                                                                                                       cccAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGAACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTgc                                       >  2:399722/1‑150 (MQ=255)
                                                                                                        ccAAACCCGCGCAACAGCAGTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCAgcg                                      >  1:916565/1‑150 (MQ=255)
                                                                                                              ccGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGcc                                >  1:861802/1‑150 (MQ=255)
                                                                                                                   cAACAGCATTCTGTTCTCCGGCTTTCAAGCCGATGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGc                           <  2:466837/150‑1 (MQ=255)
                                                                                                                       aGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCtgat                       <  2:6885/150‑1 (MQ=255)
                                                                                                                           ttCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCTGATGaaa                   <  1:368001/150‑1 (MQ=255)
                                                                                                                              tGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCTGATGAAATCa                >  2:1030360/1‑150 (MQ=255)
                                                                                                                               gTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCTGATGAAATCAt               <  2:974395/150‑1 (MQ=255)
                                                                                                                               gTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGCATGTGCCTATCCGTGCACAGGTGCATGCCATGGAGAATCTTTCTGCGCACGCCGATGCTGATGAAATCAt               <  2:1164871/150‑1 (MQ=255)
                                                                                                                                ttCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCTGATGAAATCATg              <  2:1144416/150‑1 (MQ=255)
                                                                                                                                ttCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCTGATGAAATCATg              <  2:887273/150‑1 (MQ=255)
                                                                                                                                 cccccGGCTTGAAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCTGATGAAATCATgg             >  1:751032/4‑150 (MQ=255)
                                                                                                                                  ccccGGCTTGAAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCTGATGAAATCATGGa            >  1:718768/3‑150 (MQ=255)
                                                                                                                                         tttCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCt                                             <  2:1275946/110‑1 (MQ=255)
                                                                                                                                         tttCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCt                                             >  1:1275946/1‑110 (MQ=255)
                                                                                                                                            cAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCTGATGAAATCATGGAATGGTTgcgc  >  1:861887/1‑150 (MQ=255)
                                                                                                                                            |                                                                                                                                                     
ATGAGTCCAGGCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCTGATGAAATCATGGAATGGTTGCGC  >  NC_002947/3726319‑3726608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑