Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,774,290 C→G 9.4% A317G (GCC→GGC)  PP_5743 → TonB‑dependent receptor protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,774,2900CG 9.4% 152.9 / 6.6 53A317G (GCC→GGC) PP_5743TonB‑dependent receptor protein
Reads supporting (aligned to +/- strand):  ref base C (24/24);  new base G (2/3);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-01

CCAGATAACGCTTGCCGGCTTCGGTCAGCGCGATGCGTCGGGTGGTGCGGTTGAGCAGGCGAATGTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTGGGCAAAGGGTGCAAGGACGGCGGCCGGCCACCGTCAACTGATCATGGTTTAGCCGGGCACCGGCAGCCAGC  >  NC_002947/3774141‑3774436
                                                                                                                                                     |                                                                                                                                                  
ccAGATAACGCTTGCCGGCTTCGGTCAGCGCGATGCGTCGGGTGGTGCGGTTGAGCAGGCGAATGTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGc                                                                                                                                                    <  2:85890/150‑1 (MQ=255)
ccAGATAACGCTTGCCGGCTTCGGTCAGCGCGATGCGTCGGGTGGTGCGGTTGAGCAGGCGAATGTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGc                                                                                                                                                    <  2:653581/150‑1 (MQ=255)
ccAGATAACGCTTGCCGGCTTCGGTCAGCGCGATGCGTCGGGTGGTGCGGTTGAGCAGGCGAATGTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGc                                                                                                                                                    <  2:315833/150‑1 (MQ=255)
    aTAACGCTTGCCGGCTTCGGTCAGCGCGATGCGTCGGGTGGTGCGGTTGAGCAGGCGAATGTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGc                                                                                                                                                <  2:1117733/150‑1 (MQ=255)
     tAACGCTTGCCGGCTTCGGTCAGCGCGATGCGTCGGGTGGTGCGGTTGAGCAGGCGAATGTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGcc                                                                                                                                               <  1:305503/150‑1 (MQ=255)
         gCTTGCCGGCTTCGGTCAGCGCGATGCGTCGGGTGGTGCGGTTGAGCAGGCGAATGTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGGCCGCCTGac                                                                                                                                           <  2:709134/150‑2 (MQ=255)
                 gCTTCGGTCAGCGCGATGCGTCGGGTGGTGCGGTTGAGCAGGCGAATGTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAg                                                                                                                                   <  1:981415/150‑1 (MQ=255)
                  cTTCGGTCAGCGCGATGCGTCGGGTGGTGCGGTTGAGCAGGCGAATGTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGt                                                                                                                                  <  1:1116918/150‑1 (MQ=255)
                   ttCGGTCAGCGCGATGCGTCGGGTGGTGCGGTTGAGCAGGCGAATGTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGtt                                                                                                                                 <  2:835984/150‑1 (MQ=255)
                       gTCAGCGCGATGCGTCGGGTGGTGCGGTTGAGCAGGCGAATGTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAAc                                                                                                                                          >  1:1112935/1‑137 (MQ=255)
                       gTCAGCGCGATGCGTCGGGTGGTGCGGTTGAGCAGGCGAATGTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAAc                                                                                                                                          <  2:1112935/137‑1 (MQ=255)
                               gATGCGTCGGGTGGTGCGGTTGAGCAGGCGAATGTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCAt                                                                                                                     >  2:645377/1‑150 (MQ=255)
                                       gggTGGTGCGGTTGAGCAGGCGAATGTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGaa                                                                                                             <  2:480900/150‑1 (MQ=255)
                                              gCGGTTGAGCAGGCGAATGTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTAcatc                                                                                                      >  1:315550/1‑150 (MQ=255)
                                                ggTTGAGCAGGCGAATGTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTAcatcca                                                                                                    >  1:993274/1‑150 (MQ=255)
                                                        aGGCGAATGTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGt                                                                                            >  1:566952/1‑150 (MQ=255)
                                                        aGGCGAATGTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGt                                                                                            >  1:292738/1‑150 (MQ=255)
                                                          gCGAATGTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGaa                                                                                                             >  2:675610/1‑131 (MQ=255)
                                                          gCGAATGTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGaa                                                                                                             <  1:675610/131‑1 (MQ=255)
                                                            gAATGTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAAcc                                                                                        >  1:1224388/1‑150 (MQ=255)
                                                                    aGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGtt                                                                                                                                 >  1:156893/1‑101 (MQ=255)
                                                                    aGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGtt                                                                                                                                 <  2:156893/101‑1 (MQ=255)
                                                                    aGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTg                                                                                <  2:933502/150‑1 (MQ=255)
                                                                           ccTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGACTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTg                                                                         >  1:956372/1‑150 (MQ=255)
                                                                            cTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGaa                                                                                                             >  1:1326428/1‑113 (MQ=255)
                                                                            cTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGaa                                                                                                             <  2:1326428/113‑1 (MQ=255)
                                                                            cTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTgg                                                                        >  1:393602/1‑150 (MQ=255)
                                                                                 ggTTCGACAGCAGCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTGGGCaaa                                                                   <  1:645377/150‑1 (MQ=255)
                                                                                   ttCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTGGGCAAAgg                                                                 >  1:750981/1‑150 (MQ=255)
                                                                                      gACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTGGGCAAAGGGTg                                                              >  2:1140779/1‑150 (MQ=255)
                                                                                            aTCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAAcgc                                                                                                                           <  2:70741/83‑1 (MQ=255)
                                                                                            aTCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAAcgc                                                                                                                           >  1:70741/1‑83 (MQ=255)
                                                                                             tCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTGGGCAAAGGGTGCAAGGAc                                                       >  1:954647/1‑150 (MQ=255)
                                                                                             tCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTGGGCAAAGGGTGCAAGGAc                                                       >  2:863771/1‑150 (MQ=255)
                                                                                                             cGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTGGGCAAAGGGTGCAAGGACGGCGGCCGGCCACCGt                                       >  2:955150/1‑150 (MQ=255)
                                                                                                              gtggtggtgTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTGGGCAAAGGGTGCAAGGACGGTGGCCGGCCGCCGTc                                      <  2:292738/150‑1 (MQ=255)
                                                                                                                 gtggtgTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTGGGCAAAGGGTGCAAGGACGGCGGCCGGCCACCGTCAAc                                   >  2:953495/1‑150 (MQ=255)
                                                                                                                  tggtgTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTGGGCAAAGGGTGCAAGGACGGCGGCCGGCCACCGTCAACt                                  >  2:1271578/1‑150 (MQ=255)
                                                                                                                    gtgtCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTGGGCAAAGGGTGCAAGGACGGCGGCCGGCCACCGTCAACTGa                                <  2:393602/150‑1 (MQ=255)
                                                                                                                        ccAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTGGGCAAAGGGTGCAAGGACGGCGGCCGGCCACCGTCAACTGATCAt                            <  2:1317670/150‑1 (MQ=255)
                                                                                                                            gTATTCGGCCGCACCCGTCACGCAGCGAATCAGAACCACTTAGTTGAACTCGCTCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTGGGCAAAGGGTGCAAGGACGGCGGCCGGCCACCGTCAACTGATCATGGtt                        <  2:1224388/150‑1 (MQ=255)
                                                                                                                             tATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTGGGCAAAGGGTGCAAGGACGGCGGCCGGCCACCGTCAACTGATCATGGttt                       <  1:978014/150‑1 (MQ=255)
                                                                                                                                  ggCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGGTTGAAGTACATCCATCGTAGGTAACCTCATGTTGGAGCTTGGGCAAAGGGTGCAAGGACGGCGGCCGGCCACCGTCAACTGATCATGGTTTAGCCg                  >  1:31484/1‑150 (MQ=255)
                                                                                                                                   gCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTGGGCAAAGGGTGCAAGGACGGCGGCCGGCCACCGTCAACTGATCATGGTTTAGCCgg                 <  2:315550/150‑1 (MQ=255)
                                                                                                                                        aCCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTGGGCaaa                                                                   <  1:535512/95‑1 (MQ=255)
                                                                                                                                        aCCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTGGGCaaa                                                                   >  2:535512/1‑95 (MQ=255)
                                                                                                                                          ccGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTGGGCAAAgg                                                                 >  2:1363942/1‑95 (MQ=255)
                                                                                                                                          ccGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTGGGCAAAgg                                                                 <  1:1363942/95‑1 (MQ=255)
                                                                                                                                            ttCAGGCTGGCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTGGGCAAAgg                                                                 >  2:1273103/2‑93 (MQ=255)
                                                                                                                                            ttCAGGCTGGCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTGGGCAAAgg                                                                 <  1:1273103/92‑1 (MQ=255)
                                                                                                                                            gTCAGGCTGCCAGCCTGACCCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTCGGGCAAAGGGTGCAAGGACGGCGGCCGGCCACCGTCAACTGATCATGGTTTAGCCGGGCACCGGca        <  1:1140779/150‑1 (MQ=255)
                                                                                                                                            gTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTGGGCAAAGGGTGCAAGGACGGCGGCCGGCCACCGTCAACTGATCATGGTTTAGCCGGGCACCGGca        <  2:956372/150‑1 (MQ=255)
                                                                                                                                              cAGGCTGGCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTGGGCAAAGGGTGCAAGGACGGcggccg                                               >  2:382985/1‑109 (MQ=255)
                                                                                                                                              cAGGCTGGCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTGGGCAAAGGGTGCAAGGACGGcggccg                                               <  1:382985/109‑1 (MQ=255)
                                                                                                                                                 gCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTGGGCAAAGGGTGCAAGGACGGCGGCCGGCCACCGTAAACTGATCATGGTTTAGCCGGGCACCGGcagccag   >  1:991106/1‑150 (MQ=255)
                                                                                                                                                  cTGCCAGCCTGACCCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTGGGCAAAGGGTGCAAGGACGGCGGCCGGCCACCGTCAACTGATCATGGTTTAGCCGGGCACCGGcagccagc  <  1:801813/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CCAGATAACGCTTGCCGGCTTCGGTCAGCGCGATGCGTCGGGTGGTGCGGTTGAGCAGGCGAATGTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTGGGCAAAGGGTGCAAGGACGGCGGCCGGCCACCGTCAACTGATCATGGTTTAGCCGGGCACCGGCAGCCAGC  >  NC_002947/3774141‑3774436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑