Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,786,381 C→T 7.5% G291S (GGC→AGC)  mhpT ← 3‑(3‑hydroxy‑phenyl)propionate transporter MhpT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,786,3810CT 7.5% 150.4 / 2.2 53G291S (GGC→AGC) mhpT3‑(3‑hydroxy‑phenyl)propionate transporter MhpT
Reads supporting (aligned to +/- strand):  ref base C (28/21);  new base T (2/2);  total (30/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

ACCTGGGTCGAATACAGCGTCGGCGCCAGCGCGTACAGCACCAACTGGCCACCAATGGCGCAATACCCTGCAGTGAACCCGGCCAGCAACATCAGCCCGAAACTGCTCGACAGCCCCAGCCCCGCCAACGACGCCAGCATGCCGGCGTAGGCGATCAATACCGCCCGCCCGGCAAACCCGCGGTCCATCATCCGACCGGTCAGGAACGATCCAGCCGCCCCACCCAGGTTGAACAGAATCTGCACGGCGCCCGCCTGCGGCCGGCTGAAGCCTTGGCCGATCAA  >  NC_002947/3786239‑3786522
                                                                                                                                              |                                                                                                                                             
aCCTGGGTCGAATACAGCGTCGGCGCCAGCGCGTACAGCACCAACTGGCCACCAATGGCGCAATACCCTGCAGTGAACCCGGCCAGCAACATCAGCCCGAAACTGCTCGACAGCCCCAGCCCCGCCAACGACGCCAGCATGCCGGCGTAg                                                                                                                                        >  1:1351844/1‑150 (MQ=255)
 ccTGGGTCGAATACAGCGTCGGCGCCAGCGCGTACAGCACCAACTGGCCACCAATGGCGCAATACCCTGCAGTGAACCCGGCCAGCAACATCAGCCCGAAACTGCTCGACAGCCCCAGCCCCGCCAACGACGCCAGCATGCCGGCGTAgg                                                                                                                                       >  1:1075284/1‑150 (MQ=255)
  cTGGGTCGAATACAGCGTCGGCGCCAGCGCGTACAGCACCAACTGGCCACCAATGGCGCAATACCCTGCAGTGAACCCGGCCAGCAACATCAGCCCGAAACTGCTCGACAGCCCCAGCCCCGCCAACGACGCCAGCATGTCg                                                                                                                                              <  1:1161741/142‑1 (MQ=255)
  cTGGGTCGAATACAGCGTCGGCGCCAGCGCGTACAGCACCAACTGGCCACCAATGGCGCAATACCCTGCAGTGAACCCGGCCAGCAACATCAGCCCGAAACTGCTCGACAGCCCCAGCCCCGCCAACGACGCCAGCATGTCg                                                                                                                                              >  2:1161741/1‑142 (MQ=255)
  cTGGGTCGAATACAGCGTCGGCGCCAGCGCGTACAGCACCAACTGGCCACCAATGGCGCAATACCCTGCAGTGAACCCGGCCAGCAACATCAGCCCGAAACTGCTCGACAGCCCCAGCCCCGCCAACGACGCCAGCATGCCGGCGTAGGc                                                                                                                                      <  1:634517/150‑1 (MQ=255)
    gggTCGAATACAGCGTCGGCGCCAGCGCGTACAGCACCAACTGGCCACCAATGGCGCAATACCCTGCAGTGAACCCGGCCAGCAACATCAGCCCGAAACTGCTCGACAGCCCCAGCCCCGCCAACGACGCCGGCATGCTGGCGtcgttgg                                                                                                                                    <  1:117364/150‑7 (MQ=255)
       tCGAATACAGCGTCGGCGCCAGCGCGTACAGCACCAACTGGCCACCAATGGCGCAATACCCTGCAGTGAACCCGGCCAGCAACATCAGCCCGAAACTGCTCGACAGCCCCAGCCCCGCCAACGACGCCAGCATGCCGGCGTAGGCGATCa                                                                                                                                 >  1:572630/1‑150 (MQ=255)
          taTACAGCGACCGCGCCAGCGCGTACAGCACCAACTTGCCCCCAATGGCGCAATACCCTGCAGAGAACCCGGCCAGCAACATCAGCCCGAAACTGCTCGACAGCCCCAGACCCCCCAACGACGCCAGCATGCCGGCGTAGGCGATCAATa                                                                                                                              <  2:148169/149‑1 (MQ=255)
           aTACAGCGTCGGCGCCAGCGCGTACAGCACCAACTGGCCACCAATGGCGCAATACCCTGCAGTGAACCCGGCCAGCAACATCAGCCCGAAACTGCTCGACAGCCCCAGCCCCGCCAACGACGCCAGCATGCCGGCGTAGGCGATCAATAc                                                                                                                             >  1:129124/1‑150 (MQ=255)
              cAGCGTCGGCGCCAGCGCGTACAGCACCAACTGGCCACCAATGGCGCAATACCCTGCAGTGAACCCGGCCAGCAACATCAGCCCGAAACTGCTCGACAGCCCCAGCCCCGCCAACGACGCCAGCATGCCGGCGTAGGCGATCAATAcaga                                                                                                                          >  1:148169/1‑147 (MQ=255)
                  gTCGGCGCCAGCGCGTACAGCACCAACTGGCCACCAATGGCGCAATACCCTGCAGTGAACCCGGCCAGCAACATCAGCCCGAAACTGCTCGACAGCCCCAGCCCCGCCAACGACGCCAGCATGCCGGCGTAGGCGATCAATAccgcccgc                                                                                                                      >  2:604999/1‑150 (MQ=255)
                       cgcCAGCGCGTACAGCACCAACTGGCCACCAATGGCGCAATACCCTGCAGTGAACCCGGCCAGCAACATCAGCCCGAAACTGCTCGACAGCCCCAGCCCCGCCAACGACGCCAGCATGCCGGCGTAGGCGATCAATACCGCCCGCCCGGc                                                                                                                 <  2:572630/150‑1 (MQ=255)
                          cAGCGCGTACAGCACCAACTGGCCACCAATGGCGCAATACCCTGCAGTGAACCCGGCCAGCAACATCAGCCCGAAACTGCTCGACAGCCCCAGCCCCGCCAACGACGCCAGCATGCCGGCGTAGGCGa                                                                                                                                    <  2:594180/128‑1 (MQ=255)
                          cAGCGCGTACAGCACCAACTGGCCACCAATGGCGCAATACCCTGCAGTGAACCCGGCCAGCAACATCAGCCCGAAACTGCTCGACAGCCCCAGCCCCGCCAACGACGCCAGCATGCCGGCGTAGGCGa                                                                                                                                    >  1:594180/1‑128 (MQ=255)
                            gcgcGTACAGCACCAACTGGCCACCAATGGCGCAATACCCTGCAGTGAACCCGGCCAGCAACATCAGCCCGAAACTGCTCGACAGCCCCAGCCCCGCCAACGACGCCAGCATGCCGGCGTAGGCGATCAATACCGCCCGCCCGGCAAAcc                                                                                                            >  2:1225813/1‑150 (MQ=255)
                              gcgTACAGCACCAACTGGCCACCAATGGCGCAATACCCTGCAGTGAACCCGGCCAGCAACATCAGCCCGAAACTGCTCGACAGCCCCAGCCCCGCCAACGACGCCAGCATGCCGGCGTAGGCGATCAATACCGCCCGCCCGGCAAACCcg                                                                                                          >  1:861556/1‑150 (MQ=255)
                                  aCAGCACCAACTGGCCACCAATGGCGCAATACCCTGCAGTGAACCCGGCCAGCAACATCAGCCCGAAACTGCTCGACAGCCCCAGCCCCGCCAACGACGCCAGCATGCCGGCGTAGGCGATCAATACCGCCCGCCCGGCAAACCCGCGGt                                                                                                      <  1:1225813/150‑1 (MQ=255)
                                      cACCAACTGGCCACCAATGGCGCAATACCCTGCAGTGAACCCGGCCAGCAACATCAGCCCGAAACTGCTCGACAGCCCCAGCCCCGCCAACGACGCCAGCATGCCGGCGTAGGCGATCAATACCGCCCGCCCGGCAAACCCGCGGTCcat                                                                                                  >  2:1278607/1‑150 (MQ=255)
                                                 caccaATGGCGCAATACCCTGCAGTGAACCCGGCCAGCAACATCAGCCCGAAACTGCTCGACAGCCCCAGCCCCGCCAACGACGCCAGCATGCCGGCGTAGGCGATCAATACCGCCCGCCCGGCAAACCCGCGGTCCATCATCCGACCgg                                                                                       >  1:1147538/1‑150 (MQ=255)
                                                  accaATGGCGCAATACCCTGCAGTGAACCCGGCCAGCAACATCAGCCCGAAACTGCTCGACAGCCCCAGCCCCGCCAACGACGCCAGCATGCCGGCGTAGGCGATCAATACCGCCCGCCCGGCAAACCCGCGGTCCATCATCCGACCGGt                                                                                      >  1:755600/1‑150 (MQ=255)
                                                   ccaATGGCGCAATACCCTGCAGTGAACCCGGCCAGCAACATCAGCCCGAAACTGCTCGACAGCCCCAGCCCCGCCAACGACGCCAGCATGCCGGCGTAGGCGATCAATACCGCCCGCCCGGCAAACCCGCGGTCCatcatc                                                                                              <  1:434176/141‑1 (MQ=255)
                                                   ccaATGGCGCAATACCCTGCAGTGAACCCGGCCAGCAACATCAGCCCGAAACTGCTCGACAGCCCCAGCCCCGCCAACGACGCCAGCATGCCGGCGTAGGCGATCAATACCGCCCGCCCGGCAAACCCGCGGTCCatcatc                                                                                              >  2:434176/1‑141 (MQ=255)
                                                    caATGGCGCAATACCCTGCAGTGAACCCGGCCAGCAACATCAGCCCGAAACTGCTCGACAGCCCCAGCCCCGCCAACGACGCCAGCATGCCGGCGTAGGCGATCAATACCGCCCGCCCGGCAAACCCGCGGTCCATCATCCGACCGGTCa                                                                                    <  2:1075284/150‑1 (MQ=255)
                                                        ggCGCAATACCCTGCAGTGAACCCGGCCAGCAACATCAGCCCGAAACTGCTCGACAGCCCCAGCCCCGCCAACGACGCCAGCATGCCGGCGTAGGCGATCAATACCGCCCGCCCGGCAAACCCGCGGTCCATCATCCGACCGGTCAGGaa                                                                                <  2:1145299/150‑1 (MQ=255)
                                                         gcgcAATACCCTGCAGTGAACCCGGCCAGCAACATCAGCCCGAAACTGCTCGACAGCCCCAGCCCCGCCAACGACGCCAGCATGCCGGCGTAGGCGATCAATACCGCCCGCCCGGCAAACCCGCGGTCCATCATCCGACCGGTCAGGAAc                                                                               >  2:956547/1‑150 (MQ=255)
                                                           gcAATACCCTGCAGTGAACCCGGCCAGCAACATCAGCCCGAAACTGCTCGACAGCCCCAGCCCCGCCAACGACGCCAGCATGCCGGCGTAGGCGATCAATACCGCCCGCCCGGCAAACCCGCGGTCCATCATCCGACCGGTCAGGAACGa                                                                             <  2:1043087/150‑1 (MQ=255)
                                                                 cccTGCAGTGAACCCGGTCAGCAACATCAGCCCGAAACTGCTCGACAGCCCCAGCCCCGCCAACGACGCCAGCATGCCGGCGTAGGCGATCAATACCGCCCGCCCGGCAAACCCGCGGTCAATCATCCGACCGGTCAGGAACGATCCAgc                                                                       >  2:529014/1‑150 (MQ=255)
                                                                  ccTGCAGTGAACCCGGCCAGCAACATCAGCCCGAAACTGCTCGACAGCCCCAGCCCCGCCAACGACGCCAGCATGCCGGCGTAGGCGATCAATACCGCCCGCCCGGCAAACCCGCGGTCCATCATCCGACCGGTCAGGAACGATCCAgcc                                                                      >  2:732278/1‑150 (MQ=255)
                                                                   cTGCAGTGAACCCGGCCAGCAACATCAGCCCGAAACTGCTCGACAGCCCCAGCCCCGCCAACGACGCCAGCATGCCGGCGTAGGCGATCAATACCGCCCGCCCGGCAAACCCGCGGTCCATCATCCGACCGGTCAGGAACGATCCAgccg                                                                     >  1:465618/1‑150 (MQ=255)
                                                                         ggAACCCGGCCAGCAACATCAGCCCGAAACTGCTCGACAGCCCCAGCCCCGCCAACGACGCCAGCATGCCGGCGTAGGCGATCAATACCGCCCGCCCGGCAAACCCGCGGTCCATCATCCGACCGGTCAGGAACGATCCAGCCGCcccac                                                               <  2:129124/149‑1 (MQ=255)
                                                                           aaCCCGGCCAGCAACATCAGCCCGAAACTGCTCGACAGCCCCAGCCCCGCCAACGACGCCAGCATGCCGGCGTAGGCGATCAATACCGCCCGCCCGGCAAACCCGCGGTCCATCATCCGACCGGTCAGGaa                                                                                <  1:816519/131‑1 (MQ=255)
                                                                           aaCCCGGCCAGCAACATCAGCCCGAAACTGCTCGACAGCCCCAGCCCCGCCAACGACGCCAGCATGCCGGCGTAGGCGATCAATACCGCCCGCCCGGCAAACCCGCGGTCCATCATCCGACCGGTCAGGaa                                                                                >  2:816519/1‑131 (MQ=255)
                                                                               cGGCCAGCAACATTATCCCGAAACTACTAGACAGCCCCAGCCGCGCCAACGACGCCATCATGCCGGCGTAGGCGATCAATACCGCCCGCCCGGCAAACCCGCGGTCCATCATCCGACCGGTCAGGAACGATCCAGCCGCCCCACCCAGGt                                                         <  2:663298/150‑1 (MQ=255)
                                                                               cGGCCAGCAACATCAGCCCGAAACTGCTCGACAGCCCCAGCCCCGCCAACGACGCCAGCATGCCGGCGTAGGCGATCAATACCGCCCGCCCGGCAAACCCGCGGTCCATCATCCGACCGGTCAGGAACGATCCAGCCGCCCCACCCAGGt                                                         <  2:1147538/150‑1 (MQ=255)
                                                                                ggCCAGCAAAATCAGCCCCAACCTGCCCGACAGCCCCAGCCCCGCCAACGCCGCCAGCATGCCGGCGTAGGCGATCAATACCGCCCGCCCGGCAAACCCGCGGTCCATCACCCGTCCGGTCAGGAACGATCCAGCCGCCCCACCCAGGtt                                                        <  2:264937/150‑1 (MQ=255)
                                                                                   cAGCAACATCAGCCCGAAACTGCTCGACAGCCCCAGCCCCGCCAACGACGCCAGCATGCCGGCGTATGCGATCAATACCGCCCGCCCGGCAAACCCGCGGTCCATCATCCGACCGGTCAGGAACGATCCAGCCGCCCCACCCAGGTTGaa                                                     <  2:755600/150‑1 (MQ=255)
                                                                                               cccGAAACTGCTCGACAGCCCCAGCCCCGCCAACGACGCCAGCATGCCGGCGTAGGCGATCAATAc                                                                                                                             <  1:1169673/66‑1 (MQ=255)
                                                                                               cccGAAACTGCTCGACAGCCCCAGCCCCGCCAACGACGCCAGCATGCCGGCGTAGGCGATCAATAc                                                                                                                             >  2:1169673/1‑66 (MQ=255)
                                                                                                      cTGCTCGACAGCCCCAGCCCCGCCAACGACGCCAGCATGCCGGCGTAGGCGATCAATACCGCCCGCCCGGCAAACCCGCGGTCCATCATCCGACCGGTCAGGAACGATCCAGCCGCCCCACCCAGGTTGAACAGAATCTGCACGGcgccc                                  <  1:624424/150‑1 (MQ=255)
                                                                                                       tGCTCGACAGCCCCAGCCCCGCCAACGACGCCAGCATGCCGGCGTAGGCGATCAATACCGCCCGCCCGGCAAACCCGCGGTCCATCATCCGACCGGTCAGGAACGATCCAGCCGCCCCACCCAGGTTGAACAGAATCTGCACGGcgcccg                                 >  2:377723/1‑150 (MQ=255)
                                                                                                            gACAGCCCCAGCCCCGCCAACGACGCCAGCATGCCGGCGTAGGCGATCAATACCGCCCGCCCGGCAAACCCGCGGTCCATCATCCGACCGGTCAGGAACGATCCAGCCGCCCCACCCAGGTTGAACAGAATCTGCACGGCGCCCGCCTGc                            >  1:923211/1‑150 (MQ=255)
                                                                                                                 ccccagccccGCCAACGACGCCAGCATGCCGGCGTAGGCGATCAATACCGCCCGCCCGGCAAACCCGCGGTCCATCATCCGACCGGTCAGGAACGATCCAGCCGCCCCACCCAGGTTGaa                                                     >  1:1393082/1‑120 (MQ=255)
                                                                                                                 ccccagccccGCCAACGACGCCAGCATGCCGGCGTAGGCGATCAATACCGCCCGCCCGGCAAACCCGCGGTCCATCATCCGACCGGTCAGGAACGATCCAGCCGCCCCACCCAGGTTGaa                                                     <  2:1393082/120‑1 (MQ=255)
                                                                                                                  cccagccccGCCAACGACGCCAGCATGCCGGCGTAGGCGATCAATACCGCCCGCCCGGCAAACCCGCGGTCCATCATCCGACCGGTCAGGAACGATCCAGCCGCCCCACCCAGGTTGAACAGAATCTGCACGGCGCCCGCCTGcggccgg                      <  1:1278607/150‑1 (MQ=255)
                                                                                                                         ccGCCAACGACGCCAGCATGCCGGCGTAGGCGATCAATACCGCCCGCCCGGCAAACCCGCGGTCCATCATCCGACCGGTCAGGAACGATCCAGCCGCCCCACCCAGGTTGAACAGAATCTGCACGGCGCCCGCCTGCGGCCGGCTGAAGc               >  2:1102274/1‑150 (MQ=255)
                                                                                                                          cGCCAACGACGCCAGCATGCCGGCGTAGGCGATCAATACCGCCCGCCCGGCAAACCCGCGGTCCATCATCCGACCGGTCAGGAACGATCCAGCCGCCCCACCCAGGTTGAACAGAATCTGCACGGCGCCCGCCTGCGGCCGGCTGAAGcc              >  1:209246/1‑150 (MQ=255)
                                                                                                                          cGCCAACGACGCCAGCATGCCGGCGTAGGCGATCAATACCGCCCGCCCGGCAAACCCGCGGTCCATCATCCGACCGGTCAGGAACGATCCAGCCGCCCCACCCAGGTTGAACAGAATCTGCACGGCGCCCGCCTGCGGCCGGCTGAAGcc              >  1:833034/1‑150 (MQ=255)
                                                                                                                              aaCGACGCCAGCATGCCGGCGTAGGCGATCAATACCGCCCGCCCGGCAAACCCGCGGTCCATCATCCGACCGGTCAGGAACGATCCAGCCGCCCCACCCAGGTTGAACAGAATCTGCACGGCGCCCGCCTGCGGCCGGCTGCAGCCTTgg          >  1:60239/1‑150 (MQ=255)
                                                                                                                               cctacgCCGGCATGCTGGCGTAGGCGATCAATACCGCCCGCCCGGCAAACCCGCGGTCCATCATCCGACCGGTCAGGAACGATCCAGCCGCCCCACCCAGGTTGAACAGAATCTGCACg                                        >  1:1211131/4‑119 (MQ=255)
                                                                                                                               cctacgCCGGCATGCTGGCGTAGGCGATCAATACCGCCCGCCCGGCAAACCCGCGGTCCATCATCCGACCGGTCAGGAACGATCCAGCCGCCCCACCCAGGTTGAACAGAATCTGCACg                                        <  2:1211131/116‑1 (MQ=255)
                                                                                                                               acgacgCCAGCATGCCGGCGTAGGCGATCAATACCGCCCGCCCGGCAAACCCGCGGTCCATCATCCGACCGGTCAGGAACGATCCAGCCGCCCCACCCAGGTTGAACAGAATCTGCACGGCGCCCGCCTGCGGCCGGCTGAAGCCTTGGc         <  2:833034/150‑1 (MQ=255)
                                                                                                                                 gacgCCAGCATGCCGGCGTAGGCGATCAATACCGCCCGCCCGGCAAACCCGCGGTCCATCATCCGACCGGTCAGGAACGATCCAGCCGCCCCACCCAGGTTGAACAGAATCTGCACGGCGCCCGCCTGCGGCCGGCTGAAGCCTTGGCCg       >  1:1388158/1‑150 (MQ=255)
                                                                                                                                      cggCATGCTGGCGTAGGCGATCAATACCGCCCGCCCGGCAAACCCGCGGTCCATCATCCGACCGGTCAGGAACGATCCAGCCGCCCCACCCAGGTTGAACAGAATCTGCACGGCGCCCGCCTGCGGCCGGCTGAAGCCTTGACCGAACaa  >  2:254797/3‑150 (MQ=255)
                                                                                                                                              cGGCGTAGGCGATCAATACCGCCCGCCCGGCAAACCCGCGGTCCATCATCCGACCGGTCAGGAACGATCCAGCCGCCCCACCCAGGTTGAACAGAATCTGCACGGCGCCCGCCTGCGGCCGGCTGAAGCCTTgg          <  1:759455/134‑1 (MQ=255)
                                                                                                                                              cGGCGTAGGCGATCAATACCGCCCGCCCGGCAAACCCGCGGTCCATCATCCGACCGGTCAGGAACGATCCAGCCGCCCCACCCAGGTTGAACAGAATCTGCACGGCGCCCGCCTGCGGCCGGCTGAAGCCTTgg          >  2:759455/1‑134 (MQ=255)
                                                                                                                                              |                                                                                                                                             
ACCTGGGTCGAATACAGCGTCGGCGCCAGCGCGTACAGCACCAACTGGCCACCAATGGCGCAATACCCTGCAGTGAACCCGGCCAGCAACATCAGCCCGAAACTGCTCGACAGCCCCAGCCCCGCCAACGACGCCAGCATGCCGGCGTAGGCGATCAATACCGCCCGCCCGGCAAACCCGCGGTCCATCATCCGACCGGTCAGGAACGATCCAGCCGCCCCACCCAGGTTGAACAGAATCTGCACGGCGCCCGCCTGCGGCCGGCTGAAGCCTTGGCCGATCAA  >  NC_002947/3786239‑3786522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑