Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,811,902 T→C 7.8% Y61Y (TAT→TAC)  PP_3368 → MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,811,9020TC 7.8% 183.3 / 4.9 64Y61Y (TAT→TAC) PP_3368MFS transporter
Reads supporting (aligned to +/- strand):  ref base T (26/33);  new base C (2/3);  total (28/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.08e-01

CGTCGCCAAGATGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGATGCTGCTGTATGCCGTGGGCAACCTGGCCACTGCCTTTGCCCCCTCGTTCGGTGGC  >  NC_002947/3811761‑3812040
                                                                                                                                             |                                                                                                                                          
cGTCGCCAAGATGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTggg                                                                                                                                    >  1:195172/1‑150 (MQ=255)
cGTCGCCAAGATGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTggg                                                                                                                                    <  1:711842/150‑1 (MQ=255)
cGTCGCCAAGATGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCATACGCGCTgat                                                                                                                                    <  1:953561/150‑3 (MQ=255)
cGTCGCCAAGATGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCATACGCGCTgat                                                                                                                                    <  1:953465/150‑3 (MQ=255)
 gTCGCCAAGATGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCATACGCGCTgatg                                                                                                                                   <  2:1030550/150‑4 (MQ=255)
     ccAAGATGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTaa                                                                                                                               >  2:997766/1‑150 (MQ=255)
       aaGATGGCGGCCGCCAGGGCACTGGGCAGCTTTGGCATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGTGCTGGGGGTAATg                                                                                                                             <  2:747151/150‑1 (MQ=255)
       aaGATGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATg                                                                                                                             <  1:1419611/150‑1 (MQ=255)
        aGATGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATgg                                                                                                                            >  1:395013/1‑150 (MQ=255)
         gATGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAATTCGCCATCATGTGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGt                                                                                                                           <  1:708809/150‑1 (MQ=255)
          aTGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTgggg                                                                                                                                   >  1:986934/1‑141 (MQ=255)
          aTGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTgggg                                                                                                                                   <  2:986934/141‑1 (MQ=255)
                  cgccATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGcccc                                                                                                                  >  1:61514/1‑150 (MQ=255)
                             tGGGCAGCTTTGCCATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCa                                                                                                       <  2:1421878/150‑1 (MQ=255)
                             tGGGCAGCTTTGCCATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCa                                                                                                       >  1:592786/1‑150 (MQ=255)
                                    cTTTGCCATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGCTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCgg                                                                                                >  1:1394622/1‑150 (MQ=255)
                                     tttGCCATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATAAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCg                                                                                                 >  2:1023855/1‑148 (MQ=255)
                                     tttGCCATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATAAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCg                                                                                                 <  1:1023855/148‑1 (MQ=255)
                                          cATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTgg                                                                                                                                     >  1:504121/1‑107 (MQ=255)
                                          cATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTgg                                                                                                                                     <  2:504121/107‑1 (MQ=255)
                                           aTCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGGCATTCTCGGTGCCaaa                                                                                         >  2:925399/1‑150 (MQ=255)
                                                cACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGCTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAActgct                                                                                    <  2:1394622/150‑1 (MQ=255)
                                                  ccGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACtgctgc                                                                                  <  1:513923/150‑1 (MQ=255)
                                                   cGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGc                                                                                             >  2:353645/1‑138 (MQ=255)
                                                   cGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGc                                                                                             <  1:353645/138‑1 (MQ=255)
                                                     gCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCa                                                                               >  2:1191096/1‑150 (MQ=255)
                                                      cGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCaa                                                                              <  1:1172857/150‑1 (MQ=255)
                                                       gAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAg                                                                             <  1:618712/150‑1 (MQ=255)
                                                       gAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAg                                                                             <  1:618755/150‑1 (MQ=255)
                                                             gCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACAtg                                                                       >  1:290559/1‑150 (MQ=255)
                                                             gCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACAtg                                                                       >  2:795430/1‑150 (MQ=255)
                                                               catcatGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACAtgct                                                                     >  2:786368/1‑150 (MQ=255)
                                                                 tcatGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACAtgctgc                                                                   <  1:997766/150‑1 (MQ=255)
                                                                     gggCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATgctgctgct                                                               >  2:422617/1‑150 (MQ=255)
                                                                       gCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGc                                                                                             >  1:219697/1‑118 (MQ=255)
                                                                       gCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGc                                                                                             <  2:219697/118‑1 (MQ=255)
                                                                       gCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGt                                                             <  1:1191096/150‑1 (MQ=255)
                                                                       gCCTGATGAAAGACATTGTCCGCCATTTCGCGTTGAGCGTTCCGCAGGGGGGCTATGCCATCAGCGTGGATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGt                                                             >  2:728384/1‑150 (MQ=255)
                                                                         cTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGt                                                                                               >  1:579955/1‑114 (MQ=255)
                                                                         cTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGt                                                                                               <  2:579955/114‑1 (MQ=255)
                                                                          tGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGa                                                          <  2:776852/150‑1 (MQ=255)
                                                                            aTGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGAtg                                                        <  1:562807/150‑1 (MQ=255)
                                                                            aTGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGAtg                                                        <  2:195172/150‑1 (MQ=255)
                                                                                ccGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGAtgctgc                                                    >  2:240105/1‑150 (MQ=255)
                                                                                ccGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGAtgctgc                                                    >  1:571688/1‑150 (MQ=255)
                                                                                ccGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGAtgctgc                                                    >  2:811163/1‑150 (MQ=255)
                                                                                ccGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGAtgctgc                                                    <  2:571688/150‑1 (MQ=255)
                                                                                 cGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATTTTCTCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGATgctgct                                                   >  1:812339/1‑150 (MQ=255)
                                                                                          cAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCGAAGGTGGTGCCATTCTCGGTGCCAAACTGCTGAGCAAGCACACGCGGCTGCTGTTGATGCTGCTGTATGCCGt                                          >  1:1314942/1‑150 (MQ=255)
                                                                                           aGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCGGCGCAAGCACATGCTGCTGCTGTTGATGCTGCTGTATGCCGTg                                         <  2:395013/150‑1 (MQ=255)
                                                                                            gCACTTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGATGCTGCTGTATGCCGTgg                                        <  2:812339/150‑1 (MQ=255)
                                                                                            gCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGATGCTGCTGTATGCCGTgg                                        <  1:786368/150‑1 (MQ=255)
                                                                                                    cAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGATGCTGCTGTATGCCGTGGGCAACCTg                                <  2:61514/150‑1 (MQ=255)
                                                                                                      gTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGATGCTGCTGTATGCCGTGGGCAACCTGGc                              >  2:284514/1‑150 (MQ=255)
                                                                                                                ccGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGATGCTGCTGTATGCCGTGGGCAACCTGGCCACTGCCt                      <  1:204730/148‑1 (MQ=255)
                                                                                                                ccGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGATGCTGCTGTATGCCGTGGGCAACCTGGCCACTGCCt                      >  2:204730/1‑148 (MQ=255)
                                                                                                                  gCAGGTGGGCCTTGCCTTCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCACCCCGCTGCGCAAGCCCATGCTACTGCTGTTGATGCTGCTGTATGCCGTGGGCAACCTGGCCACTGCCTTTGc                  <  1:811163/150‑1 (MQ=255)
                                                                                                                  gCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGATGCTGCTGTATGCCGTGGGCAACCTGGCCACTGCCTTTGc                  <  1:942989/150‑1 (MQ=255)
                                                                                                                      gTGGGCCATGCCATTAGCGGGTATGCGCTGGGGGTAATGGTTGGCGCTCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGATGCTGCTGTATGCCGTGGGCAACCTGGCCACTGCCTTTGccccc              <  2:8544/150‑1 (MQ=255)
                                                                                                                      gTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGATGCTGCTGTATGCCGTGGGCAACCTGGCCACTGCCTTTGccccc              <  2:261752/150‑1 (MQ=255)
                                                                                                                           ccatgccatCAGCGCGTATGCGCTGGGGGTAATGGTTGTCGCCCCGACACTGGAACTGCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGATGCTGCTGTATGCCGTGGGCAACCTGGCCACTGCCTTTGCCCCCTCGtt         <  1:728384/150‑1 (MQ=255)
                                                                                                                               gccatCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGATGCTGCTGTATGCCGTGGGCAACCTGGCCACTGCCTTTGCCCCCTCGTTCGGt     <  1:240105/150‑1 (MQ=255)
                                                                                                                                 ccccAGCGCATACGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGATGCTGCTGTATGCCGTGGGCAACCTGGCCACTGCCTTTGCCCCCTCGTTCGGTgg   >  1:694201/4‑150 (MQ=255)
                                                                                                                                  cccAGCGCATACGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGATGCTGCTGTATGCCGTGGGCAACCTGGCCACTGCCTTTGCCCCCTCGTTCGGTGGc  >  2:1352089/3‑150 (MQ=255)
                                                                                                                                             |                                                                                                                                          
CGTCGCCAAGATGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGATGCTGCTGTATGCCGTGGGCAACCTGGCCACTGCCTTTGCCCCCTCGTTCGGTGGC  >  NC_002947/3811761‑3812040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑