Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,812,195 C→G 12.4% P159R (CCG→CGG)  PP_3368 → MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,812,1950CG12.4% 149.5 / 11.1 55P159R (CCG→CGG) PP_3368MFS transporter
Reads supporting (aligned to +/- strand):  ref base C (25/23);  new base G (4/3);  total (29/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

GCATTTCGTTTCATCAGCGGCCTGCCCCACGGCGCCTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGGGGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTGCCCCAGCGCCACGACGAAACACGCAGCGACCCGCGCAAGGAGCTCCA  >  NC_002947/3812047‑3812342
                                                                                                                                                    |                                                                                                                                                   
gCATTTCGTTTCATCAGCGGCCTGCCCCACGGCGCCTAC‑TCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGGGGGCCGTTGCCCGGGTGGTGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCgg                                                                                                                                                   >  1:416553/1‑150 (MQ=255)
  aTTTCGTTTCATCAGCGGCCTGCCCCACGGCGCCTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGGGGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGt                                                                                                                                                  >  1:739815/1‑150 (MQ=255)
   tttCGTTTCATCAGCGGCCTGCCCCACGGCGCCTACTTCGGCTTTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCCGCGCGCGGGGGCCGTTGCCCGGGTGATGAGGGGCCTGACCTTGGCCATGTTGCTCGGCACCCCGGtt                                                                                                                                                 <  2:663985/150‑1 (MQ=255)
   tttCGTTTCATCAGCGGCCTGCCCCACGGCGCCTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGGGGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGtt                                                                                                                                                 <  2:739815/150‑1 (MQ=255)
      cGTTTCATCAGCGGCCTGCCCCACGGCGCCTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGGGGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGcc                                                                                                                                              <  2:297347/150‑1 (MQ=255)
        tttCATCAGCGGCCTGCCCCACGGCGCCTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGGGGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCAc                                                                                                                                            >  2:861392/1‑150 (MQ=255)
          tcatcaGCGGCCTGCCCCACGGCGCCTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGGGGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCGGGTTGccgagc                                                                                                                                          <  1:573994/150‑5 (MQ=255)
            atcaGCGGCCTGCCCCACGGCGCCTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCGGAGACCGGGGGACGTTGCCCGGGTTGTGGTGGGCCGGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTc                                                                                                                                        >  2:1229550/1‑150 (MQ=255)
                     cTGCCCCACGGCGCCTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGGGGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAg                                                                                                                               >  1:868907/1‑150 (MQ=255)
                     cTGCCCCACGGCGCCTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGGGGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAg                                                                                                                               <  2:137135/150‑1 (MQ=255)
                      tGCCCCACGGCGCCTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGGGGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGt                                                                                                                              <  1:1222997/150‑1 (MQ=255)
                          ccACGGCGCCTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGGGGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACtt                                                                                                                          <  2:868907/150‑1 (MQ=255)
                             cGGCGCCTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGGGGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCgg                                                                                                                       >  1:62651/1‑150 (MQ=255)
                                cgcCTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGGGGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTg                                                                                                                    <  1:1308836/150‑1 (MQ=255)
                                  ccTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGGGGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGc                                                                                                                  >  1:242156/1‑150 (MQ=255)
                                  ccTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGGGGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGc                                                                                                                  <  2:242156/150‑1 (MQ=255)
                                  accactgcGGCAATGCCGAAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGGGGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGc                                                                                                                  >  2:564282/8‑150 (MQ=255)
                                   cTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGGGGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCg                                                                                                                 >  1:417682/1‑150 (MQ=255)
                                                 ccGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGGGGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGc                                                                                                   <  1:927166/150‑1 (MQ=255)
                                                       tggtggCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGGGGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCg                                                                                             >  1:660551/1‑150 (MQ=255)
                                                                 gAGCATGGTCGCCAAGGATCAGCGCGCGGGGGCCGTTGCCCGGGTGATGATGGGCCCGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCg                                                                                                             >  1:1412572/1‑124 (MQ=255)
                                                                 gAGCATGGTCGCCAAGGATCAGCGCGCGGGGGCCGTTGCCCGGGTGATGATGGGCCCGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCg                                                                                                             <  2:1412572/124‑1 (MQ=255)
                                                                  aGCATGGTCGCCAAGGATCAGAGCGCGGGGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGcc                                                                                  <  2:594146/150‑1 (MQ=255)
                                                                   gCATGGTCGCCAAGGATCAGCGCGCGGGGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGccc                                                                                 <  1:417957/150‑1 (MQ=255)
                                                                    cATGGTCGCCAAGGATCAGCGCGCGGGGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCt                                                                                <  1:687057/150‑1 (MQ=255)
                                                                      tGGTCGCCAAGGATCAGCGCGCGGGGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTGCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCtgt                                                                              <  1:564282/150‑1 (MQ=255)
                                                                       ggTCGCCAAGGATCAGCGCGCGGGGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTg                                                                                                    >  2:916257/1‑127 (MQ=255)
                                                                       ggTCGCCAAGGATCAGCGCGCGGGGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTg                                                                                                    <  1:916257/127‑1 (MQ=255)
                                                                        gTCGCCAAGGATCAGCGCGCGGGGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGc                                                                            >  1:1037734/1‑150 (MQ=255)
                                                                         tCGCCAAGGATCAGCGCGCGGGGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTgg                                                                                                <  2:1332132/129‑1 (MQ=255)
                                                                         tCGCCAAGGATCAGCGCGCGGGGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTgg                                                                                                >  1:1332132/1‑129 (MQ=255)
                                                                         tCGCCAAGGATCAGCGCGCGGGGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGgc                                                                                           <  1:122773/134‑1 (MQ=255)
                                                                         tCGCCAAGGATCAGCGCGCGGGGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGgc                                                                                           >  2:122773/1‑134 (MQ=255)
                                                                                   tCAGCGCGCGGGGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGc                                                                                                   >  1:1051899/1‑116 (MQ=255)
                                                                                   tCAGCGCGCGGGGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGc                                                                                                   <  2:1051899/116‑1 (MQ=255)
                                                                                      gcgcgcGGGGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTg                                                                                                    >  2:1341606/1‑112 (MQ=255)
                                                                                      gcgcgcGGGGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTg                                                                                                    <  1:1341606/112‑1 (MQ=255)
                                                                                        gcgcgGGGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCt                                                            <  1:972576/150‑1 (MQ=255)
                                                                                          gcgGGGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTgg                                                          <  1:1228812/150‑1 (MQ=255)
                                                                                                gCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTc                                                    >  1:253623/1‑150 (MQ=255)
                                                                                                  cGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGCTCGCCCTGTGCACCATCGCCCTGGTCTttcgtttcgt                                                  >  2:793532/1‑150 (MQ=255)
                                                                                                   ggggCCGGGGGGATGATGGGCCTGGCCTTGGCGATGTTGCTCGGCAGCCGGGTTGCCACCTTCCTGGGCCAGTACTTGGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTg                                                 <  2:253623/147‑1 (MQ=255)
                                                                                                   gTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTg                                                 >  1:662751/1‑150 (MQ=255)
                                                                                                                        cTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCAGCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGGGCCCCA‑CGGCACGGCGCAacac                           >  1:626343/1‑150 (MQ=255)
                                                                                                                          gACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGCGAGGGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTGCCCCAGCGCGACGACGAAACACg                          >  1:385987/1‑150 (MQ=255)
                                                                                                                             cTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTGCCCCAGCGCCACGACGAAACACGCAg                       >  2:294287/1‑150 (MQ=255)
                                                                                                                                     tGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTGCCCCAGCGCCACGACGAAACACGCAGCGACCcgc               <  1:321238/150‑1 (MQ=255)
                                                                                                                                     tGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTGCCCCAGCGCCACGACGAAACACGCAGCGACCcgc               <  1:321175/150‑1 (MQ=255)
                                                                                                                                       ttGCTCGGCAACCCGGTTGCCACCTTCCTGGGCTACGACTGCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTGCCCCAGCGCCACGACGAAACACGCAGCGACCCgcgc             >  1:1403994/1‑150 (MQ=255)
                                                                                                                                        tGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTGCCCCAGCGCCACGACGAAACACGCAGCGACCCGCGCa            >  1:478070/1‑150 (MQ=255)
                                                                                                                                          ggtggCAACCGGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTGCCCCAGCGCCACGACGAAACACGCAGCGACCCGCGCAAg          >  2:200538/4‑150 (MQ=255)
                                                                                                                                           gtggCAACCGGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATc                                                                      <  2:383480/87‑1 (MQ=255)
                                                                                                                                           gtggCAACCGGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATc                                                                      >  1:383480/3‑89 (MQ=255)
                                                                                                                                           gtggCAACCGGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTGCCCCAGCGCCACGACGAAACACGCAGCGACCCGCGCAAgg         >  2:35238/3‑150 (MQ=255)
                                                                                                                                             ggCAACCGGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTGCCCCAGCGCCACGACGAAACACGCAGCGACCCGCGCAAGGAg       >  2:1383246/1‑150 (MQ=255)
                                                                                                                                                  cccGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTGCCCCAGCGCCACGACGAAACACGCAGCGACCCGCGCAAGGAGCTCCa  <  1:861392/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCATTTCGTTTCATCAGCGGCCTGCCCCACGGCGCCTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGGGGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTGCCCCAGCGCCACGACGAAACACGCAGCGACCCGCGCAAGGAGCTCCA  >  NC_002947/3812047‑3812342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑