| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,866,973 | C→A | 21.0% | intergenic (‑52/‑155) | PP_3415 ← / → gnuK | LacI family transcriptional regulator/D‑gluconate kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,866,973 | 0 | C | A | 21.0% | 101.2 / 17.9 | 48 | intergenic (‑52/‑155) | PP_3415/gnuK | LacI family transcriptional regulator/D‑gluconate kinase |
| Reads supporting (aligned to +/- strand): ref base C (13/24); new base A (4/6); total (18/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
CGCGCGAAGCGGTAATCGGGGAAACCCCGGAAAGCCTGGCAACTTCGGCCAGGGTGGGACGACCTGTAGTTCGGGAGCCGGTGCGGGACATGAATGTTGTAATTTTACTACTTGCAAGGAGAGGGAACGGCCACTAAGGTAGCGCTGTCTCAGGATGCAGGCAATGTAATCTTTGGTAGCAGCCACCACGCGCTCGCCACCAGGCGTCCATACTGCGTAAGGACAAGACCAATAACACCGGGGAGGTGGCTGTTCGTCATCGGCGAGACAGCGCTATCTCACCCGC > NC_002947/3866831‑3867116 | cgcgcgAAGCGGTAATCGGGGAAACCCCGGAAAGCCTGGCAACTTCGGCCAGGGTGGGACGACCTGTAGTTCGGGAGCCGGTGCGGGACATGAATGTTGTAATTTTACTACTTGCAAGGAGAGGGAACGGCCACTAAGGTAGAGCTGtct > 1:856551/1‑150 (MQ=255) ggTAATCGGGGAAACCCCGGAAAGCCTGGCAACTTCGGCCAGGGTGGGACGACCTGTAGTTCGGGAGCCGGTGCGGGACATGAATGTTGTAATTTTACTACTTGCAAGGAGAGGGAACGGCCACTAAGGTAGCGCTGTCTCAGGATGCAg < 1:917198/150‑1 (MQ=255) gTAATCGGGGAAACCCCGGAAAGCCTGGCAACTTCGGCCAGGGTGGGACGACCTGTAGTTCGGGAGCCGGTGCGGGACATGAATGTTGTAATTTTACTACTTGCAAGGAGAGGGAACGGCCACTAAGGTAGAGCTGTCTCAGGATGCAgg > 1:701762/1‑150 (MQ=255) tCGGGGAAACCCCGGAAAGCCTGGCAACTTCGGCCAGGGTGGGACGACCTGTAGTTCGGGAGCCGGTGCGGGACATGAATGTTGTAATTTTACTACTTGCAAGGAGAGGGAACGGCCACTAAGGTAGCGCTGTCTCAGGATGCAGGctca > 2:721971/1‑147 (MQ=255) tCGGGGAAACCCCGGAAAGCCTGGCAACTTCGGCCAGGGTGGGACGACCTGTAGTTCGGGAGCCGGTGCGGGACATGAATGTTGTAATTTTACTACTTGCAAGGAGAGGGAACGGCCACTAAGGTAGCGCTGTCTCAGGATGCAGGCAAt < 1:436525/150‑1 (MQ=255) ggggAAACCCCGGAAAGCCTGGCAACTTCGGCCAGGGTGGGACGACTTGTAGTTCGGGAGCCGGTGCGGGACATGAATGTTGTAATTTTACTACTTGCAAGGAGAGGGAACGGCCACTAAGGTAGCGCTGTCTCAGGATGCAGGCAATGt > 2:496624/1‑150 (MQ=255) gggAAACCCCGGAAAGCCTGGCAACTTCGGCCAGGGTGGGACGACCTGTAGTTCGGGAGCCGGTGCGGGACATGAATGTTGTAATTTTACTACTTGCAAGGAGAGGGAACGGCCACTAAGGTAGCGCTGTCTCAGGATGCAGGAAATGTa > 1:501809/1‑150 (MQ=255) gAAACCCCGGAAAGCCTGGCAACTTCGGCCAGGGTGGGACGACCTGTAGTTCGGGAGCCGGTGCGGGACATGAATGTTGTAATTTTACTACTTGCAAGGAGAGGGAACGGCCACTAAGGTAGCGCTGTCTCAGGATGCAGGCAATGTAAt < 1:331332/150‑1 (MQ=255) aCCCCGGAAAGCCTGGCAACTTCGGCCAGGGTGGGACGACCTGTAGTTCGGGAGCCGGTGCGGGACATGAATGTTGTAATTTTACTACTTGCAAGGAGAGGGAACGGCCACTAAGGTAGCGCTGTCTCAGGATGCAGGCAATGTAATCtt > 1:1015260/1‑150 (MQ=255) ccccGGAAAGCCTGGCAACTTCGGCCAGGGTGGGACGACCTGTAGTTCGGGAGCCGGTGCGGGACATGAATGTTGTAATTTTACTACTTGCAAGGAGAGGGAACGGCCACTAAGGTAGCGCTGTCTCAGGATGCAGGCAATGTAATCttt < 2:496477/150‑1 (MQ=255) ccccGGAAAACCTGGCAACTTCGGCCAGGGTGGGACGACCTGTAGTTCGGGAGCCGGTGCGTGACATGAATGTTGTAATTTTACTACTTGCAAGGAGAGGTAACGGCCACTACGGTAGCGCTGTCTCAGGATGCAGGCAATGTAATCttt < 2:496447/150‑1 (MQ=255) cccGGAAAGCCTTGCAACTTCGGCCAGGGTGGGACGACCTGTAGTTCGCGAGCCGGTGCGGGACATGAATGTTGTAATTTTACTACTTGCAAGGAGAGGGAACGGCCACTAAGGTAGAGCTGTCTCAGGATGCAGGCAATGTAATCTTTg < 1:131616/150‑1 (MQ=255) cGGAAAGCCTGGCAACTTCGGCCAGGGTGGGACGACCTGTAGTTCGGGAGCCGGTGCGGGACATGAATGTTGTAATTTTACTACATGCAAGGAGAGGGAACGGCCACTAAGGTAGCGCTGTCTCAGGATGCAGGCAATGTAATCTTTGGt > 1:1393598/1‑150 (MQ=255) aaGCCTGGCAACTTCGGCTAGGGTGGGACGACCTGTAGTTCGGGAGCCGGTGCGGGACATGAATGTTGTAATTTTACTACTTGCAAGGAGAGGGAACGGAAACTAAGGTCGCGATGTCTGACGAGGCGGCCAAGGTAAGCGTGGGGagca < 2:15568/150‑1 (MQ=255) aaGCCTGGCAACTTCGGCCAGGGTGGGACGACCTGTAGTTCGGGAGCCGGTGCGGGACATGAATGTTGTAATTTTACTACTTGCAAGGAGAGGGAACGGCCACTAAGGTAGCGCTGTCTCAGGATGCAGGCAATGTAATCTTTGGTagca < 1:178753/150‑1 (MQ=255) aaGCCTGGCAACTTCGGCCAGGGTGGGACGACCTGTAGTTCGGGAGCCGGTGCGGGACATGAATGTTGTAATTTTACTACTTGCAAGGAGAGGGAACGGCCACTAAGGTAGAGCTGTCTCAGGATGCAGGCAATGTAATCTTTGGTagca < 2:701762/150‑1 (MQ=255) aaGCCGGGCAACGGCGGACAGGGTGGGACGACCTGTAGTTCGGGAGCCGGTGCGGGACATGAATGTTGTAATTTTACTACTTGCAAGGAGAGGGAACGGCCACTAAGGTAGAGCTGTCTCAGGATGCAGGCAATGTAATCTTTGGTagca < 2:112274/150‑1 (MQ=255) ttCGGCCAGGGTGGGACGACCTGTAGTTCGGGAGCCGGTGCGGGACATGAATGTTGTAATTTTACTACTTGCAAGGAGAGGGAACGGCCACTAAGGTAGCGCTGTCTCAGGATGCAGGCAATGTAATCTTTGGTAGCAGCCACCACgcgc > 1:79563/1‑150 (MQ=255) ttgCCAGGGTGGGACGACCTGTAGTTCGGGAGCCGGTGCGGGACATGAATGTTGTAATTTTACTACTTGCAAGGAGAGGGAACGGCCACTAAGGTAGCGCTGTCTCAGGATGCAGGCAATGTAATCTTTGGTAGCAGCCACCACGCGCTc < 1:719080/148‑1 (MQ=255) gCCAGGGTGGGACGACCTGGAGTTCGGGAGCCGGTGCGGGACATGAATGTTGTAATTTTACTACTTGCAAGGAGAGGGAACGGCCACTAAGGTAGCGCTGTCTCAGGATGCAGGCAATGTAATCTTTGGTAGCAGCCACCACGCGCTCGc < 1:81573/150‑1 (MQ=255) gTGGGACGACCTGTAGTTCGGGAGCCGGTGCGGGACATGAATGTTGTAATTTTACTACTTGCAAGGAGAGGGAACGGCCACTAAGGTAgcg > 2:1144311/1‑91 (MQ=255) gTGGGACGACCTGTAGTTCGGGAGCCGGTGCGGGACATGAATGTTGTAATTTTACTACTTGCAAGGAGAGGGAACGGCCACTAAGGTAgcg < 1:1144311/91‑1 (MQ=255) gTGGGACGACCTGTAGTTCGGGAGCCGGTGCGGGACATGAATGTTGTAATTTTACTACTTGCAAGGAGAGGGAACGGCCACTAAGGTAGCGCTGTCTCAGGATGCAGGCAATGTAATCTTTGGTAGCAGCCACCACGCGCTCGCCACCAg < 1:662806/150‑1 (MQ=255) gacgacCTGTAGTTCGGGAGCCGGTGCGGGACATGAATGTTGTAATTTTACTACTTGCAAGGAGAGGGAACGGCCACTAAGGTAGCGCTGTCTCAGGATGCAGGCAATGTAATCTTTGGTAGCAGCCACCACGCGCTCGCCACCAGGCGt < 1:1158371/150‑1 (MQ=255) acgtcCTGTAGGTCGGGAGCCGGTGCGGGACATGAATGTTGGAATTTTACTACTTGCAAGGAGAGGGAACGGCCACTAAGGTAGCGCTGTCTCAGGATGCAGGCAATGTAATCTTTGGTAGCAGCCACCACGCGCTCGCCACCAGGCGTc < 2:79563/150‑1 (MQ=255) gacCTGTAGTTCGGGCGCCGGTGCGGAATATCAAAAAGTTGATTTTACGACTTTCAAGGAGAGGGAACGGCCACTAAGGTAGAGCTGTCTCAGGATGCAGGCAATGTAATCTTTGGTAGCAGCCACCACGCGCTCGCCACCAGGCGTCCa < 2:1224538/150‑1 (MQ=255) ccTGTAGTTCGGGAGCCGGTGCGGGACATGAATGTTGTAATTTTACTACTTGCAAGGAGAGGGAACGGCCACTAAGGTAGCGCTGTCTCAGGATGCAGGCAATGTAATCTTTGGTAGCAGCCACCACGCGCTCGCCACCAGGCGTCCATa < 2:246552/150‑1 (MQ=255) ccTGTAGTTCGGGAGCCGGTGCGGGACATGAATGTTGTAATTTTACTACATGCAAGGAGAGGGAACGGCCACTAAGGTAGCGCTGTCTCAGGATGCAGGCAATGTAATCTTTGGTAGCAGCCACCACGCGCTCGCCACCAGGCGTCCATa < 2:1393598/150‑1 (MQ=255) cTGTAGTTCGGGAGCCGGTGCGGGACATGAATGTTGTAATTTTACTACTTGCAAGGAGAGGGAACGGCCACTAAGGTAGCGCTGTCTCAGGATGCAGGCAATGTAATCTTTGGTAGCAGccac > 1:404457/1‑123 (MQ=255) cTGTAGTTCGGGAGCCGGTGCGGGACATGAATGTTGTAATTTTACTACTTGCAAGGAGAGGGAACGGCCACTAAGGTAGCGCTGTCTCAGGATGCAGGCAATGTAATCTTTGGTAGCAGccac < 2:404457/123‑1 (MQ=255) cGGGAGCCGGTGCTGGAAATGAAGGTTGTAATTTTAGTTGTTGCAAGGAGAGTGAACGGCCACTAAGGTAGCGCTGTCTCAGGATGCAGGCCATGTAATCTTTGGTAGCAGCCACCACGCGCTCGCCACCACGCGTCCATACTGCGTAAg < 2:473016/150‑1 (MQ=255) gCCGGTGCGGGACATGAATGTTGTAATTTTACTACTTGCAAGGAGAGGGAACGGCCACTAAGGTAGCGCTGTCTCAGGATGCAGGCAATGTAATCTTTg > 2:1376523/1‑99 (MQ=255) gCCGGTGCGGGACATGAATGTTGTAATTTTACTACTTGCAAGGAGAGGGAACGGCCACTAAGGTAGCGCTGTCTCAGGATGCAGGCAATGTAATCTTTg < 1:1376523/99‑1 (MQ=255) gCCGGTGCGGGACATGAATGTTGTAATTTTACTACTTGCAAGGAGAGGGAACGGCCACTAAGGTAGAGCTGTCTCAGGATGCAGGCAATGTAATCTTTGGTAGCAGCCACCACGCGCTCGCCACCAGGCGTCCATACTGCGTAAGGACaa < 1:280434/150‑1 (MQ=255) cGGGACATTAATGTTGTAATTTTACCACTTCCAAAGAAAGCGAACGGCCACTAAGGTAGCCCCGACTCAGGATGCAGGCAATGTAATCTTTGGTAGCAGCCACCACGCGCTCGCCACCAGGCGTCCATACTGCGTAAGGACAAGACCAAt < 1:595211/150‑1 (MQ=255) aTGAATGTTGTAATTTTACTACTTGCAAGGAGAGGGAACGGCCACTAAGGTAGCGCTGTCTCAGGa < 1:874300/66‑1 (MQ=255) aTGAATGTTGTAATTTTACTACTTGCAAGGAGAGGGAACGGCCACTAAGGTAGCGCTGTCTCAGGa > 2:874300/1‑66 (MQ=255) aaTGTTGTAATTTTACTACTTGCAAGGAGAGGGAACGGCCACTAAGGTAGCGCTGTCTCAGGATGCAGGCAATGTAATCTTTGGTAGCAGCCACCACGCGCTCGCCACCAGGCGTCCATACTGCGTAAGGACAAGACCAATAACACCggg < 1:254081/150‑1 (MQ=255) tgtAATTTTACTACTTGCAAGGAGAGGGAACGGCCACTAAGGTAGAGCTGTCTCAGGATGCAGGCAATGTAATCTTTGGTAGCAGCCACCACGCGCTCGCCACCAGGCGTCCATACTGCGTAAGGACAAGACCAATAACACCGGGGAGGt > 1:732125/1‑150 (MQ=255) gtAATTTTACTACTTGCAAGGAGAGGGAACGGCCACTAAGGTAGAGCTGTCTCAGGATGCAGGCAATGTAATCTTTGGTAGCAGCCACCACGCGCTCGCCACCAGGCGTCCATACTGCGTAAGGACAAGACCAATAACACCGGGGAGGTg < 2:856551/150‑1 (MQ=255) tAATTTTACTACTTGCAACGAGCGGAAACGTCAACTAAGGTAGCGCTGTCTCAGGATGCAGGCAATGTAATCTTTGGTACCAGCCACCACGCGCTCGCCACCAGGCGTCCATACTGCGTAAGGACAAGACCAATAACACCGGGGAGGTgg < 2:1398713/150‑1 (MQ=255) ttttACTACTTGCAAGGAGAGGGAACGGCCACTAAGGTAGCGCTGTCTCAGGATGCAGGCAATGTAATCTTTGGTAGCAGCCACCACGCGCTCGCCACCAGGCGTCCATACTGCGTAAGGACAAGACCAATAACACCGGGGAGGTGGCTg > 1:648024/1‑150 (MQ=255) tttACTACTTGCAAGGAGAGGGAACGGCCACTAAGGTAGCGCTGTCTCAGGATGCAGGCAATGTAATCTTTGGTAGCAGCCACCACGCGCTCGCCACCAGGCGTCCATACTGCGTAAGGACAAGACCAATAACACCGGGGAGGTGGCTGt < 1:496624/150‑1 (MQ=255) gCAAGGAGAGGGAACGGCCACTAAGGTAGCGCTGTCTCAGGATGCAGGCAATGTAATCTTTGGTAGCAGCCACCACGCGCTCGCCACCAGGCGTCCATACTGCGTAAGGACAAGa < 1:1138889/115‑1 (MQ=255) gCAAGGAGAGGGAACGGCCACTAAGGTAGCGCTGTCTCAGGATGCAGGCAATGTAATCTTTGGTAGCAGCCACCACGCGCTCGCCACCAGGCGTCCATACTGCGTAAGGACAAGa > 2:1138889/1‑115 (MQ=255) ggAGAGGGAACGGCCACTAAGGTAGCGCTGTCTCAGGATGCAGGCAATGTAATCTTTgg < 1:964880/59‑1 (MQ=255) ggAGAGGGAACGGCCACTAAGGTAGCGCTGTCTCAGGATGCAGGCAATGTAATCTTTgg > 2:964880/1‑59 (MQ=255) agGGAACGGCCACTAAGGTAGCGCTGTCTCAGGATGCAGGCAATGTAATCTTTGGTAGCAGCCACCACGCGCTCGCCACCAGGCGTCCATACTGCGTAAGGACAAGACCAATAACACCGGGGAGGTGGCTGTTCGTCATCGGCGAGACAg > 1:1325524/1‑150 (MQ=255) tAAGGTAGTGCTGTCTCAGGATGCAGGCAATGTAATCTTTGGTAGCAGCCACCACGCGCTCGCCACCAGGCGTCCATACTGCGTAAGGACAAGACCAATAACACCGGGGAGGTGGCTGTTCGTCATCGGCGAGACAGCGCTATCTCAccc > 2:883610/1‑150 (MQ=255) aGGTAGAGCTGTCTCAGGATGCAGGCAATGTAATCTTTGGTAGCAGCCACCACGCGCTCGCCACCAGGCGTCCATACTGCGTAAGGACAAGACCAATAACACCGGGGAGGTGGCTGTTCGTCATCGGCGAGACAGCGCTATCTCACCCGc > 1:818992/1‑150 (MQ=255) | CGCGCGAAGCGGTAATCGGGGAAACCCCGGAAAGCCTGGCAACTTCGGCCAGGGTGGGACGACCTGTAGTTCGGGAGCCGGTGCGGGACATGAATGTTGTAATTTTACTACTTGCAAGGAGAGGGAACGGCCACTAAGGTAGCGCTGTCTCAGGATGCAGGCAATGTAATCTTTGGTAGCAGCCACCACGCGCTCGCCACCAGGCGTCCATACTGCGTAAGGACAAGACCAATAACACCGGGGAGGTGGCTGTTCGTCATCGGCGAGACAGCGCTATCTCACCCGC > NC_002947/3866831‑3867116 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |