Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,900,163	T→G	9.1%	L176R (CTG→CGG)	PP_3443 →	glyceraldehyde‑3‑phosphate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,900,163	0	T	G	9.1%	179.6 / 10.4	66	L176R (CTG→CGG)	PP_3443	glyceraldehyde‑3‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (29/31);  new base G (3/3);  total (32/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.52e-01

CAAGAACCTGAAGGATTCGGAAAAGGAATTCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCAAGCCGGCCAAGTTCGGCGTACTGCTGATCCGACCCTTGCTCGAAGCGTT  >  NC_002947/3900017‑3900310                                                                                                                                                   |                                                                                                                                                    cAAGAACCTGAAGGATTCGGAAAAGGAATTCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTggg                                                                                                                                                  <  1:859778/150‑1 (MQ=255)     aaCCTGAAGGATTCGGAAAAGGAATTCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTg                                                                                                                                              <  1:939232/150‑1 (MQ=255)     aaCCTGAAGGATTCGGAAAAGGAATTCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTg                                                                                                                                              <  1:663252/150‑1 (MQ=255)        cTGAAGGATTCGGAAAAGGAATTCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCAGCGGGGCGCGGcg                                                                                                                                           <  1:97479/150‑2 (MQ=255)              gATTCGGAAAAGGAATTCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCACGCCCAGCGGGGCGCGGcggatctg                                                                                                                                     <  2:927484/150‑8 (MQ=255)                     aaaaGGAATTCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGc                                                                                                                              >  2:1055329/1‑150 (MQ=255)                         ggAATTCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTa                                                                                                                          <  1:895533/150‑1 (MQ=255)                          gAATTCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTAc                                                                                                                         >  1:809359/1‑150 (MQ=255)                            aTTCGACCGCACCCCCGACTAGATCGTCAACACCATCAACTCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGTCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACaa                                                                                                                       <  2:630743/150‑1 (MQ=255)                               cGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTa                                                                                                                    <  2:248523/150‑1 (MQ=255)                                gACCGCAGCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTAc                                                                                                                   <  2:102075/150‑1 (MQ=255)                                   cGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCg                                                                                                                <  2:997636/150‑1 (MQ=255)                                            gACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACgag                                                                                                       >  1:1129477/1‑150 (MQ=255)                                                    cgtcgACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTcac                                                                                               <  2:891148/150‑1 (MQ=255)                                                     gtcgACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTg                                                                                                             >  1:339925/1‑135 (MQ=255)                                                     gtcgACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTg                                                                                                             <  2:339925/135‑1 (MQ=255)                                                         acacCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGc                                                                                          >  1:52043/1‑150 (MQ=255)                                                           acCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTc                                                                                        >  2:1258478/1‑150 (MQ=255)                                                                 aaCGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCg                                                                                  >  1:223501/1‑150 (MQ=255)                                                                  aCGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCgg                                                                                 >  1:1068253/1‑150 (MQ=255)                                                                   cGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGgc                                                                                <  1:1055329/150‑1 (MQ=255)                                                                     cccTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGgcgc                                                                              >  1:467040/1‑150 (MQ=255)                                                                      ccTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCt                                                                             >  2:645441/1‑150 (MQ=255)                                                                        tCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGa                                                                           <  1:1200158/150‑1 (MQ=255)                                                                            ggACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCAt                                                                       <  2:1129477/150‑1 (MQ=255)                                                                            ggACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCAt                                                                       >  2:925634/1‑150 (MQ=255)                                                                             gACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATg                                                                      <  1:925634/150‑1 (MQ=255)                                                                               ccTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATggg                                                                    <  2:938888/150‑1 (MQ=255)                                                                                cTGGACCGCCGCTCCAGCCGCTTCGAGCTGGCGCAAGCCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGc                                                                   >  1:1316841/1‑150 (MQ=255)                                                                                cTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGc                                                                   >  1:1331824/1‑150 (MQ=255)                                                                                      cgccgcTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACg                                                             <  2:1174125/150‑1 (MQ=255)                                                                                          gcTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGATGATAATGGGCAACAgggggg                                                         >  2:1371058/1‑150 (MQ=255)                                                                                            tCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGgtggtg                                                       >  1:267543/1‑150 (MQ=255)                                                                                             ccAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGgtgt                                                      >  1:463590/1‑150 (MQ=255)                                                                                              cAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGgtgt                                                      >  1:1225984/1‑149 (MQ=255)                                                                                              cAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGgtgt                                                      <  2:1225984/149‑1 (MQ=255)                                                                                              cAGCCGCTGCGAGCGGGAGCAAGGCACCGTGGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGtt                                                     >  2:415554/1‑150 (MQ=255)                                                                                                gCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCa                                                   >  1:803007/1‑150 (MQ=255)                                                                                                 ccGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCaa                                                  <  2:1331824/150‑1 (MQ=255)                                                                                                    cTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCAAGcc                                               >  2:376918/1‑150 (MQ=255)                                                                                                     ttCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACTAATTCCCGCTTAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCAAGCCg                                              >  2:115046/1‑150 (MQ=255)                                                                                                      agcggCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATTATATGTAACACGGTGGTGTTCAAGCCgg                                             <  1:903307/146‑1 (MQ=255)                                                                                                           cTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCAAGCCGGc                                            >  1:422734/1‑146 (MQ=255)                                                                                                           cTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCAAGCCGGc                                            <  2:422734/146‑1 (MQ=255)                                                                                                            tGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACaa                                                                                                                       <  2:620348/70‑1 (MQ=255)                                                                                                            tGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACaa                                                                                                                       >  1:620348/1‑70 (MQ=255)                                                                                                             ggAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCAAGCCGGCCAAGtt                                      >  2:607703/1‑150 (MQ=255)                                                                                                                    ggCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCaa                                                  >  1:1073275/1‑131 (MQ=255)                                                                                                                    ggCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCaa                                                  <  2:1073275/131‑1 (MQ=255)                                                                                                                    ggCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCAAGCCGGCCAAGTTCGGCGTa                               <  1:1258478/150‑1 (MQ=255)                                                                                                                           tCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCAAGCCGGCCAAGTTCGGCGTACTGCTGa                        >  1:435635/1‑150 (MQ=255)                                                                                                                             ggCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCAAGCCGGCCAAGTTCGGCGTACTGCTGATc                      <  1:1371058/150‑1 (MQ=255)                                                                                                                              gCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCAAGCCGGCCAAGTTCGGCGTACTGCTGATcc                     <  1:645441/150‑1 (MQ=255)                                                                                                                                 aGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCAAGCCGGCCAAGTTCGGCGTACTGCTGATCCGAc                  >  2:643121/1‑150 (MQ=255)                                                                                                                                 aGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCAAGCCGGCCAAGTTCGGCGTACTGCTGATCCGAc                  <  2:809359/150‑1 (MQ=255)                                                                                                                                  acagggccacGCCCAGCGGGGCGCGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCAAGCCgg                                             >  2:1270035/6‑122 (MQ=255)                                                                                                                                  acagggccacGCCCAGCGGGGCGCGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCAAGCCgg                                             <  1:1270035/117‑1 (MQ=255)                                                                                                                                   aTCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGc                                                                                          <  1:922540/76‑1 (MQ=255)                                                                                                                                   aTCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTCAACGAGACCTTCACCACGc                                                                                          >  2:922540/1‑76 (MQ=255)                                                                                                                                    agggccacgcCCAGCGGGGCGCGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCAAGCCGGCCAAGTTCGGCGTACTGCTGATCCGACCCt               >  2:153098/8‑150 (MQ=255)                                                                                                                                         cgcgCCTCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACACCTTCCCGCTGAACGAGATCTTCACCACGCTCATCCCGGGGCTGATCATGGGCAACACGGTAGTGTTCAAGCCGGCCAAGTTCGGCGTACTGCTGACCCGACCCTTGCTc          <  2:492246/150‑1 (MQ=255)                                                                                                                                         cgcgCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCAAGCCGGCCAAGTTCGGCGTACTGCTGATCCGACCCTTGCTc          <  2:463590/150‑1 (MQ=255)                                                                                                                                         caggcCCAGCGGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCAAGCCGGCCAAGTTCGGCGTACTGCTGATCCGACCCTTGCTc          >  1:1423797/4‑150 (MQ=255)                                                                                                                                              cccGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATg                                                                      <  2:1151690/85‑1 (MQ=255)                                                                                                                                              cccGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATg                                                                      >  1:1151690/1‑85 (MQ=255)                                                                                                                                                 gCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCAAGCCGGCCAAGTTCGGCGTACTGCTGATCCGACCCTTGCTCGAAGCGtt  <  2:467040/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    CAAGAACCTGAAGGATTCGGAAAAGGAATTCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCAAGCCGGCCAAGTTCGGCGTACTGCTGATCCGACCCTTGCTCGAAGCGTT  >  NC_002947/3900017‑3900310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑