Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,920,317 C→T 15.0% R498Q (CGG→CAG)  acs ← acyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,920,3170CT15.0% 91.9 / 9.1 40R498Q (CGG→CAG) acsacyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base C (16/18);  new base T (4/2);  total (20/20)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

GCATCCGGAATTTCTGCACCTTGCCCGTCACCGTCATCGGGAACTCGTCGACAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAGCTCGCGCGGGTAAATGTTCTCGCCGCCGCGAATGATCATGTCC  >  NC_002947/3920171‑3920457
                                                                                                                                                  |                                                                                                                                            
gcATCCGGAATTTCTGCACCTTGCCCGTCACCGTCATCGGGAACTCGTCGACAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCa                                                                                                                                           >  1:403362/1‑150 (MQ=255)
     cGGAATTTCTGCACCTTGCCCGTCACCGTCATCGGGAACTCGTCGACAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCGGCTGCAcccgg                                                                                                                                      <  2:208901/150‑3 (MQ=255)
     cGGAATTTCTGCACCTTGCCCGTCACCGTCATCGGGAACTCGTCGACAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAg                                                                                                                                      <  2:1370184/150‑1 (MQ=255)
       gAATTTCTGCACCTTGCCCGTCACCGTCATCGGGAACTCGTCGACAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGc                                                                                                                                    <  1:1324538/150‑1 (MQ=255)
                cACCTTGCCCGTCACCGTCATCGGGAACTCGTCGACAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGAtctc                                                                                                                           <  2:1035173/150‑1 (MQ=255)
                                          agtcgtctACAAAACGGAAATACCGAGGCACCTTGAAGTGCGCTATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGc                                                                                                 <  2:234241/148‑1 (MQ=255)
                                          aCTCGTCGACAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGc                                                                                                 <  1:164386/150‑1 (MQ=255)
                                               tcgACAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATc                                                                                            <  1:697138/150‑1 (MQ=255)
                                                 gACAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGTTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCAc                                                                                          <  2:687190/150‑1 (MQ=255)
                                                  aCAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCAcc                                                                                         <  2:1100235/150‑1 (MQ=255)
                                                       aCGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGcac                                                                                    >  2:721015/1‑150 (MQ=255)
                                                           aaaTACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCGGGAGGGATAGAGCGTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCg                                                                                >  2:37394/1‑150 (MQ=255)
                                                             aTACCGGGGCAACTTGCAGTGCGAAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGc                                                                              <  2:1083787/150‑1 (MQ=255)
                                                                     gCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCAccgccg                                                                      >  2:11620/1‑150 (MQ=255)
                                                                         cTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCgggtg                                                                  >  2:908875/1‑150 (MQ=255)
                                                                          ttGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCgggtgg                                                                 <  1:1206437/150‑1 (MQ=255)
                                                                            gAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCATGCCAATCACCTGCACATCGGCCACCGCCgggtgggt                                                               <  2:403362/150‑1 (MQ=255)
                                                                                  cgcAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTgaagaa                                                         <  2:132481/150‑1 (MQ=255)
                                                                                      aTCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAAc                                                     <  2:756929/150‑1 (MQ=255)
                                                                                         cTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTgaagaagaa                                                      >  1:758876/1‑146 (MQ=255)
                                                                                         cTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTgaagaagaa                                                      <  2:758876/146‑1 (MQ=255)
                                                                                          ttGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAActcct                                                 >  1:1219404/1‑150 (MQ=255)
                                                                                               cTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAGc                                            >  1:947521/1‑150 (MQ=255)
                                                                                                  gCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAGCTcg                                         >  1:338769/1‑150 (MQ=255)
                                                                                                                ctctACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGCCCCCCCCCACCCCCCCCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAGCTCGCGCGGGTAAATGtt                           >  1:212037/1‑150 (MQ=37)
                                                                                                                 tctACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAGCTCGCGCGGGTAAATGTtc                          <  2:338769/150‑1 (MQ=255)
                                                                                                                 tctACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAGCTCGCGCGGGTAAATGTtc                          >  2:336637/1‑150 (MQ=255)
                                                                                                                   tACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAGCTCGCGCGGGTAAATGTtctc                        >  1:974475/1‑150 (MQ=255)
                                                                                                                      cTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAg                                                                                                        <  2:951991/67‑1 (MQ=255)
                                                                                                                      cTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAg                                                                                                        >  1:951991/1‑67 (MQ=255)
                                                                                                                         aCTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAActcct                                                 <  2:1211713/119‑1 (MQ=255)
                                                                                                                         aCTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAActcct                                                 >  1:1211713/1‑119 (MQ=255)
                                                                                                                             aCAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCCCAGCAGCCACCGCCGGGTGGGTGAAGAAGAACTCCTACCGCTGGGGCGGGTAAATGTTCTCGCCGCCGCGa              >  1:1380719/1‑150 (MQ=37)
                                                                                                                                       ctgggTGCGGCTGCACCCGGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCCGCTCGCGCGGGTAAATGTTCTCGCCGCCGCGAATGATCATGt    >  1:177512/3‑150 (MQ=21)
                                                                                                                                       ctgggTGCGGCTGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAActcc                                                  >  2:325510/3‑104 (MQ=255)
                                                                                                                                       ctgggTGCGGCTGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAActcc                                                  <  1:325510/102‑1 (MQ=255)
                                                                                                                                       ctgggTGCGGCTGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAGCTCGCGCGGGTAAATGTTCTCGCCGCCGCGAATGATCATGt    >  2:40433/3‑150 (MQ=255)
                                                                                                                                         gggTGCGGCTGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAGCTCGCGCGGGTAAATGTTCTCGCCGCCGCGAATGATCATGTcc  >  1:1403234/1‑150 (MQ=255)
                                                                                                                                               aGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCgggtgggt                                                               <  2:124462/83‑1 (MQ=255)
                                                                                                                                               aGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCgggtgggt                                                               >  1:124462/1‑83 (MQ=255)
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GCATCCGGAATTTCTGCACCTTGCCCGTCACCGTCATCGGGAACTCGTCGACAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAGCTCGCGCGGGTAAATGTTCTCGCCGCCGCGAATGATCATGTCC  >  NC_002947/3920171‑3920457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑