Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,934,031 A→T 16.4% C476S (TGC→AGC)  PP_3467 ← sigma‑54 dependent transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,934,0310AT16.4% 147.6 / 22.8 61C476S (TGC→AGC) PP_3467sigma‑54 dependent transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (23/28);  new base T (5/5);  total (28/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.25e-01

CTTGTCCAGCAGGCCCTGGATGATCGCCGCCAGGTCGTCGCGTTCACGCAGGGCCGGCAGGCGCAGCGACACACCGTTGACCCGGTAGAACAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCAAGGGTGCTACGCGGCGCTCCTGCAGTACCCGCAACAGGCGTGCTTGCAGGGCGAGCGGCATGTCGCCGATTTCGTCGAGGAACAGCGTGCCACCATGGGCCTGCATCA  >  NC_002947/3933882‑3934180
                                                                                                                                                     |                                                                                                                                                     
cTTGTCCAGCAGGCCCTGGATGATCGCCGCCAGGTCGTCGCGTTCACGCAGGGCCGGCAGGCGCAGCGACACACCGTTGACCCGGTAGAACAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCa                                                                                                                                                       <  1:1309408/150‑1 (MQ=255)
   gTCCAGCAGGCCCTGGATGAGCGCCGCCAGGTCGTCGCGTTCACGCAGGGCCGGCAGGCGCAGCGACACACCGTTGACCCGGTAGAACAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGAt                                                                                                                                                    <  2:983323/150‑1 (MQ=255)
      cagcagGCCCTGGATGATCGCCGCCAGGTCGTCGCGTTCACGCAGGGCCGGCAGGCGCAGCGACACACCGTTGACCCGGTAGAACAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCTGATctg                                                                                                                                                 >  2:650776/1‑148 (MQ=255)
        gcagGCCCTGGATGATCGCCGCCAGGTCGTCGCGTTCACGCAGGGCCGGCAGGCGCAGCGACACACCGTTGACCCGGTAGAACAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAgcg                                                                                                                                               <  2:1409562/150‑1 (MQ=255)
        gcagGCCCTGGATGATCGCCGCCAGGTCGTCGCGTTCACGCAGGGCCGGCAGGCGCAGCGACACACCGTTGACCCGGTAGAACAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAgcg                                                                                                                                               >  2:956891/1‑150 (MQ=255)
           ggCCCTGGATGATCGCCGCCAGGTCGTCGCGTTCACGCAGGGCCGGCAGGCGCAGCGACACACCGTTGACCCGGTAGAACAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCTGATCTGCGCCa                                                                                                                                            <  1:650776/150‑1 (MQ=255)
                tGGATGATCGCCGCCAGGTCGTCGCGTTCACGCAGGGCCGGCAGGCGCAGCGACACACCGTTGACCCGGTAGAACAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGATGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTCg                                                                                                                                       <  1:1402882/150‑1 (MQ=255)
                 ggATGATCGCCGCCAGGTCGTCGCGTTCACGCAGGGCCGGCAGGCGCAGCGACACACCGTTGACCCGGTAGAACAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTCGa                                                                                                                                      >  2:972239/1‑150 (MQ=255)
                  gatgatCGCCGCCAGGTCGTCGCGTTCACGCAGGGCCGGCAGGCGCAGCGACACACCGTTGACCCGGTAGAACAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTCGAt                                                                                                                                     <  1:618827/150‑1 (MQ=255)
                       tCGCCGCCAGGTCGTCGCGTTCACGCAGGGCCGGCAGGCGCAGCGACACACCGTTGACCCGGTAGAACAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTc                                                                                                                                        >  2:1132728/1‑142 (MQ=255)
                       tCGCCGCCAGGTCGTCGCGTTCACGCAGGGCCGGCAGGCGCAGCGACACACCGTTGACCCGGTAGAACAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTc                                                                                                                                        <  1:1132728/142‑1 (MQ=255)
                       tCGCCGCCAGGTCGTCGCGTTCACGCAGGGCCGGCAGGCGCAGCGACACACCGTTGACCCGGTAGAACAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTCGATGTCCt                                                                                                                                >  1:496462/1‑150 (MQ=255)
                             ccAGGTCGTCGCGTTCACGCAGGGCCGGCAGGCGCAGCGACACACCGTTGACCCGGTAGAACAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTCGATGTCCTGCTCGt                                                                                                                          >  2:568305/1‑150 (MQ=255)
                              cAGGTCGTCGCGTTCACGCAGGGCCGGCCGGCGCAGCGACACACCGTTGACCCGGTAGAACAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTCGATGTCCTGCTCGTc                                                                                                                         >  1:1256465/1‑150 (MQ=255)
                                  gcgtcgCGTTCACGCAGGGCCGGCAGGCGCAGCGAGACACCGTGGACCCGGTAGAACAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTAGATGTCCTGCTCGTCAccc                                                                                                                     <  2:231432/149‑1 (MQ=255)
                                     tcgCGTTCACGCAGGGCCGGCAGGCGCAGCGACACACCGTTGACCCGGTAGAACAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTCGATGTCCTGCTCGTCACCCGcc                                                                                                                  >  2:1194458/1‑150 (MQ=255)
                                       gcgTTCACGCAGGGCCGGCAGGCGCAGCGACACACCGTTGACCCGGTAGAACAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTCGATGTCCTGCTCGTCACCCGcccc                                                                                                                >  1:1366855/1‑150 (MQ=255)
                                            cACGCAGGGCCGGCAGGCGCAGCGACACACCGTTGACCCGGTAGAACAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCAAgg                                                                                                           <  1:637677/150‑1 (MQ=255)
                                               gCAGGGCCGGCAGGCGCAGCGACACACCGTTGACCCGGTAGAACAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCTGATCTGCGCCa                                                                                                                                            >  2:63281/1‑114 (MQ=255)
                                               gCAGGGCCGGCAGGCGCAGCGACACACCGTTGACCCGGTAGAACAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCTGATCTGCGCCa                                                                                                                                            <  1:63281/114‑1 (MQ=255)
                                                      cGGCAGGCGCAGCGACACACCGTTGACCCGGTAGAACAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCAAGGGTGCTAcgcg                                                                                                 <  1:940432/150‑1 (MQ=255)
                                                      cGGCAGGCGCAGCGACACACCGTTGACCCGGTAGAACAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCAAGGGTGCTAcgcg                                                                                                 <  2:1366855/150‑1 (MQ=255)
                                                             cgcAGCGACACACCGTTACCTCGGCAGAACAGGTCTTCACGGAAGTGCTGCTCCTGCACGAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTAGATGTCCTGCTCGGCACCCGCCCCCAAGGGTGCTACGCGGCGCTcc                                                                                          <  2:566763/150‑1 (MQ=255)
                                                              gcAGCGACACACCGTTGACCCGGTAGAACAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCAAGGGTGCTACGCGGCGCTCCt                                                                                         >  2:1317555/1‑150 (MQ=255)
                                                                 gCGACACACCGTTGACCCGGTAGAACAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCAAGGGTGCTACGCGGCGCTCCTGCa                                                                                      <  1:1208070/150‑1 (MQ=255)
                                                                 gCGACACACCGTTGACCCGGGAGAACAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCAAGGGTGCTACGCGGCGCTCCTGCa                                                                                      <  1:718938/150‑1 (MQ=255)
                                                                   gACACACCGTTGACCCGGTAGAACAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCAAGGGTGCTACGCGGCGCTCCTGCAGt                                                                                    >  1:512918/1‑150 (MQ=255)
                                                                              gACCCGGTAGAACAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAACGCCACGTCCATGTCCTGCTCGTCCCCCGCCCCCAAGGGTGCTACGCGGCGCTCCTGCAGTACCCGCAACAg                                                                         <  1:357986/150‑1 (MQ=255)
                                                                                ccgatctgAACAGGTCTTCACGGAAGTGCTCCTCATGGACCAGTCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCAAGGGTGCTACGCGGCGCTCCTGCAGTACCCGCAACAGGc                                                                       <  2:1045720/143‑1 (MQ=255)
                                                                                cccGGTAGAACAGGTCTTCACGGAAGTGCTGCCCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCAAGGGTGCTACGCGGCGCTCCTGCAGTAGCCGTAACAGGc                                                                       <  2:1304007/150‑1 (MQ=255)
                                                                                 ccGGTAGAACAGGTCTTCACGGAAGTGCTGCTCCTGCACGAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCAAGGGTGCTACGCGGCGCTCCTGCAGTACCCGCAACAGGCg                                                                      <  2:1256465/150‑1 (MQ=255)
                                                                                 ccGGTAGAACAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCAAGGGTGc                                                                                                       <  1:95406/117‑1 (MQ=255)
                                                                                 ccGGTAGAACAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCAAGGGTGc                                                                                                       >  2:95406/1‑117 (MQ=255)
                                                                                 ccGGTAGAACAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCAAGGGTGCTACGCGGCGCTCCTGc                                                                                       <  2:460890/133‑1 (MQ=255)
                                                                                 ccGGTAGAACAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCAAGGGTGCTACGCGGCGCTCCTGc                                                                                       >  1:460890/1‑133 (MQ=255)
                                                                                  cGGTAGAACAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCAAGGGTGCTACGCGGCGCTCCTGCAGTACCCGCAACAGGCGt                                                                     >  1:107540/1‑150 (MQ=255)
                                                                                       gAACAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCTGATCTGCGCCa                                                                                                                                            >  2:254942/1‑74 (MQ=39)
                                                                                       gAACAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCTGATCTGCGCCa                                                                                                                                            <  1:254942/74‑1 (MQ=39)
                                                                                        aaCAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCaa                                                                                                             >  2:853334/1‑104 (MQ=255)
                                                                                        aaCAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCaa                                                                                                             <  1:853334/104‑1 (MQ=255)
                                                                                        aaCAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCAAGGGTGCTACGCGGCGCTCCTGCAGTACCCGCAACAGGCgagatcgg                                                               >  1:1045720/1‑143 (MQ=255)
                                                                                         aCAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTCGAt                                                                                                                                     <  2:1223707/79‑1 (MQ=255)
                                                                                         aCAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTCGAt                                                                                                                                     >  1:1223707/1‑79 (MQ=255)
                                                                                         aCAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCAAGGGTGCTAcgcg                                                                                                 <  2:279488/115‑1 (MQ=255)
                                                                                         aCAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCAAGGGTGCTAcgcg                                                                                                 >  1:279488/1‑115 (MQ=255)
                                                                                         aCAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCAAGGGTGCTACGCGGCGCTCCTGCAGTACCCGCAACAGGCGTGCTTGCa                                                              <  1:1194458/150‑1 (MQ=255)
                                                                                             gTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCAAGGGTGCTACGCGGCGCTCCTGCAGTACCCGCAACAGGCGTGCTTGCAGGGc                                                          <  1:1317555/150‑1 (MQ=255)
                                                                                               cTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTCTATGTCCTGCTCGTCACCCGCCCCCAAGGGTGCTACGCGGCGCTCCTGCAGTACCCGCAACAGGCGTGCTTGCAGGGCGa                                                        >  1:1389841/1‑150 (MQ=255)
                                                                                                            ctgctCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCAAGGGTGCTACGCGGCGCTCCTGCAGTACCCGCAACAGGCGTGCTTGCAGGGCGAGCGGCATGTCGcc                                           <  2:1169364/150‑1 (MQ=255)
                                                                                                                tCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCAAGGGTGCTACGCGGCGCTCCTGCAGTACCCGCAACAGGCGTGCTTGCAGGGCGAGCGGCATGTCGCCGAtt                                       >  2:582552/1‑150 (MQ=255)
                                                                                                                      aCCAGGCGCTTGAGGTCGCGGTGGATGGCGCAGATCAGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCAAGGGTGCTACGCGGCGCTCCTGCAGTACCCGCAACAGGCGTGCTTGCAGGGCGAGCGGCATGTCGCCGATTtcgtcg                                 >  2:80499/1‑150 (MQ=255)
                                                                                                                        cAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCAAGGGTGCTACGCGGCGCTCCTGCAGTACCCGCAACAGGCGTGCTTGCAGGGCGAGCGGCATGTCGCCGATTTCGTCGAg                               <  2:496462/150‑1 (MQ=255)
                                                                                                                        cAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCAAGGGTGCTACGCGGCGCTCCTGCAGTACCCGCAACAGGCGTGCTTGCAGGGCGAGCGGCATGTCGCCGATTTCGTCGAg                               <  1:1210151/150‑1 (MQ=255)
                                                                                                                                 gAGGTCGCGGTGGGTTGCGCAGATCAGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCAAGGGTGCTACGCGGCGCTCCTGCAGTACCCGCAACAGGCGTGCTTGCAGGGCGAGCGGCATGTCGCCGATTTCGTCGAGGGACAGCGt                      <  1:838937/150‑1 (MQ=255)
                                                                                                                                        cgGTGGGTGGCGCAGATCAGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCAAGGGTGCTACGCGGCGCTCCTg                                                                                        <  1:81097/77‑1 (MQ=255)
                                                                                                                                        cgGTGGGTGGCGCAGATCAGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCAAGGGTGCTACGCGGCGCTCCTg                                                                                        >  2:81097/1‑77 (MQ=255)
                                                                                                                                               tggCGCTGATCTGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCAAGGGTGCTACGCGGCGCTCCTGCAGTACCCGCAACAGGCGTGCTTGCAGGGCGAGc                                                      <  1:616529/104‑1 (MQ=255)
                                                                                                                                               tggCGCTGATCTGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCAAGGGTGCTACGCGGCGCTCCTGCAGTACCCGCAACAGGCGTGCTTGCAGGGCGAGc                                                      >  2:616529/1‑104 (MQ=255)
                                                                                                                                               tggCGCTGATCTGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCAAGGGTGCTACGCGGCGCTCCTGCAGTACCCGCAACAGGCGTGCTTGCAGGGCGAGCGGCATGTCGCCGATTtcgtc                                  >  2:118178/1‑124 (MQ=255)
                                                                                                                                               tggCGCTGATCTGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCAAGGGTGCTACGCGGCGCTCCTGCAGTACCCGCAACAGGCGTGCTTGCAGGGCGAGCGGCATGTCGCCGATTtcgtc                                  <  1:118178/124‑1 (MQ=255)
                                                                                                                                                 gcgcAGATCAGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCAAGGGTGCTACGCGGCGCTCCTGCAGTACCCGCAACAGGCGTGCTTGCAGGGCGAGCGGCATGTCGCCGATTTCGTCGAGGAACAGCGTGCCACCATGGGCCTGc      >  2:707769/1‑150 (MQ=255)
                                                                                                                                                   gcAGATCAGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCAAGGGTGCTACGCGGCGCTCCTGCAGTACCCGCAACAGGCGTGCTTGCAGGGCGAGCGGCATGTCGCCGATTTCGTCGAGGAACAGCGTGCCACCATGGGCCTGCAt    >  1:1279926/1‑150 (MQ=255)
                                                                                                                                                     aGATCAGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCAAGGGTGCTACGCGGCGCTCCTGCAGTACCCGCAACAGGCGTGCTTGCAGGGCGAGCGGCATGTCGCCGATTTCGTCGAGGAACAGCGTGCCACCATGGGCCTGCATCa  <  2:107540/150‑1 (MQ=255)
                                                                                                                                                     aGATCAGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCAAGGGTGCTACGCGGCGCTCCTGCAGTACCCGCAACAGGCGTGCTTGCAGGGCGAGCGGCATGTCGCCGATTTCGTCGAGGAACAGCGTGCCACCATGGGCCTGCATCa  <  2:1279926/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTTGTCCAGCAGGCCCTGGATGATCGCCGCCAGGTCGTCGCGTTCACGCAGGGCCGGCAGGCGCAGCGACACACCGTTGACCCGGTAGAACAGGTCTTCACGGAAGTGCTGCTCCTGCACCAGGCGCTTGAGGTCGCGGTGGGTGGCGCAGATCAGCGCCACGTCGATGTCCTGCTCGTCACCCGCCCCCAAGGGTGCTACGCGGCGCTCCTGCAGTACCCGCAACAGGCGTGCTTGCAGGGCGAGCGGCATGTCGCCGATTTCGTCGAGGAACAGCGTGCCACCATGGGCCTGCATCA  >  NC_002947/3933882‑3934180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: